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2007 Fiscal Year Final Research Report Summary

Systematic study on the osteocyte network formation and their mechanical responsibility

Research Project

Project/Area Number 17209064
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTohoku University

Principal Investigator

YAMAMOTO Teruko  Tohoku University, Tohoku University Orthodontics, Professor (00127250)

Co-Investigator(Kenkyū-buntansha) KAMIOKA Hiroshi  Okayama University, 大学院・医歯薬学総合研究科, Orthodontics Associate Professor (80253219)
DEGUCHI Toru  Hospital, Orthodontics Assistant Professor (30346457)
SUGAWARA Yasuyo  Okayama University, 大学院・医歯薬学総合研究科, Orthodontics Assistant Professor (70379775)
Project Period (FY) 2005 – 2007
KeywordsOsteocyte / tooth movement / mechanical stress / Orthodontic force / three-dimensional construction / 3D-cultured primary osteocvte / Osteoblast / OB 7.3
Research Abstract

Aim: We examined mechanical property to analyze the change from osteoblasts to osteocytes. Furthermore, gap junctional intercellular communication among osteocytes in chick calvaria was examined using FRAP analysis. We established 3D cultures of osteoblast and osteocytes.
Materials & Methods: Bone cells in embryonic chick calvariae and in isolated culture were identified using fluorescently labeled phalloidin and OB7.3, a chick osteocyte-specific monoclonal antibody. The elastic modulus of living cells was analyzed with Atomic Force Microscopy. Using Fluorescence Replacement After Photobleaching (FRAP) analysis we examined the effect of changes in pH_0, [Ca^<2+>]_e, and addition of PTH on GJIC in osteocytes in chick calvaria. Anti-Connexin43 (Cx43) immunolabelling was used to localize gap junctions.
Results: The elastic modulus of peripheral regions of cells in all three populations was significantly higher than in their nuclear regions. The elastic modulus of the peripheral region of os … More teoblasts was 12053±934 Pa, that of osteoid osteocytes was 7971±422 Pa and that of mature osteocytes was 4471±198 Pa. Cx43 immunoreactivity was detected in most of the osteocyte processes. FRAP analysis showed dye-coupling among osteocytes. In untreated osteocytes, fluorescence intensity recovered 43.7±2.2% within 5 min after photobleaching. Pretreatment of osteocytes with 18 α-GA, significantly decreased. When pH_0 was decreased, fluorescence recovery significantly decreased. Conversely, when pH_0 was increased, fluorescence recovery was significantly increased. When [Ca^<2+>]_e was increased from 1 to 25 mM, fluorescence recovery was significantly decreased. In bone fragments exposed to 1.0 to 10 nM rPTH for 3 h, replacement of fluorescence was significantly increased. Chelating intracellular calcium ions affected GJIC regulation by [Ca^<2+>]_e and PTH. There were significant differences between actin and microtubule cytoskeletons in the process of MC3T3-E1 cells and primary osteocytes.
Conclusions: There were dynamic changes in the mechanical property of elastic modulus and in focal adhesions of bone cells. Furthermore, our study showed for the first time that GJIC among osteocytes is regulated by the extracellular environment and by hormonal stimulation during bone remodeling. This method may be more biologically relevant to living bone than current methods. Osteoblasts processes may have a different functional role than the osteocyte dendritic network. Less

  • Research Products

    (126 results)

All 2008 2007 2006 2005

All Journal Article (38 results) (of which Peer Reviewed: 19 results) Presentation (88 results)

  • [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes.2008

    • Author(s)
      Sugawara Y , et. al.,
    • Journal Title

      Bone (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Hormonal, pH, and calcium regulation of connexin 43-mediated dye transfer in osteocytes in chick calvaria.2008

    • Author(s)
      Ishihara Y , et. al.,
    • Journal Title

      J Bone Miner Res. 23

      Pages: 350-360

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Comparison of molecular and cellular events during lower jaw regeneration of newt (Cynops pyrrhogaster) and West African clawed frog (Xenopus tropicalis).2008

    • Author(s)
      Kurosaka H et aL,
    • Journal Title

      Dev Dyn. 237

      Pages: 354-365

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The alteration of a mechanical property of bone cells during the process of changing from osteoblasts to osteocytes2008

    • Author(s)
      Sugawara Y et. al.
    • Journal Title

      Bone (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hormonal, pH, and calcium regulation of connexin 43-mediated dye transfer in osteocytes in chick calvaria2008

    • Author(s)
      Ishihara Y et. al.
    • Journal Title

      J Bone Miner Res. 23

      Pages: 350-360

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Comparison of molecular and cellular events during lower jaw regeneration of newt (Cynops pyrrhogaster) and West African clawed frog (Xenopus tropicalis)2008

    • Author(s)
      Kurosaka H et. al.
    • Journal Title

      Dev Dyn. 237

      Pages: 354-365

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes.2007

    • Author(s)
      Murshid SA , et. al.,
    • Journal Title

      J Bone Miner Metab. 25

      Pages: 151-158

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts.2007

    • Author(s)
      Kamioka H , et. al.,
    • Journal Title

      Microsc Microanal. 13

      Pages: 108-117

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Development of a cell culture system loading cyclic mechanical strainto chondrogenic cells.2007

    • Author(s)
      Masuda T , et. al.,
    • Journal Title

      J Biotechnol. 133

      Pages: 231-238

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression and physiological role of CCN4/Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes.2007

    • Author(s)
      Yanagita T , et. al.,
    • Journal Title

      FEBS J. 274

      Pages: 151-158

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Runx3 negatively regulates 0sterix expression in dental pulp cells.2007

    • Author(s)
      Zheng L , et. al.,
    • Journal Title

      Biochem J. 405

      Pages: 69-75

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis.2007

    • Author(s)
      Yamashiro T , et. al.,
    • Journal Title

      Differentiation. 75

      Pages: 452-462

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes2007

    • Author(s)
      Murshid SA et. al.
    • Journal Title

      J Bone Miner Metab 25

      Pages: 151-158

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Primary cultures of chick osteocytes retain functional gap junctions between osteocytes and between osteocytes and osteoblasts2007

    • Author(s)
      Kamioka H et. al.
    • Journal Title

      Microsc Microanal 13

      Pages: 108-117

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of a cell culture system loading cyclic mechanical strain to chondrogenic cells2007

    • Author(s)
      Masuda T et. al.
    • Journal Title

      J Biotechnol 133

      Pages: 231-238

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression and physiological role of CCN4/Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes2007

    • Author(s)
      Yanagita T et. al.
    • Journal Title

      FEBS J. 274

      Pages: 151-158

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Runx3 negatively regulates Osterix expression in dental pulp cells2007

    • Author(s)
      Zheng L et. al.
    • Journal Title

      Biochem J 405

      Pages: 69-75

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis2007

    • Author(s)
      Yamashiro T et. al.
    • Journal Title

      Differentiation 75

      Pages: 452-462

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Roles of PKC, PI3K and JNK in multiple transduction of CCN2/CTGF signals in chondrocytes.2006

    • Author(s)
      Yosimichi G , et. al.,
    • Journal Title

      Bone 38

      Pages: 853-863

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Increase of Galanin in Trigeminal Ganglion during Tooth Movement.2006

    • Author(s)
      Deguchi T , et. al.,
    • Journal Title

      J Dent Res 85

      Pages: 658-663

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The presence of ABCG2-dependent side population cells in human periodontal ligaments2006

    • Author(s)
      Kawanabe N , et. al.,
    • Journal Title

      Biochemical and Biophysical Research Communications 344

      Pages: 1278-1283

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei.2006

    • Author(s)
      Ichikawa H , et. al.,
    • Journal Title

      Brain Research 1081

      Pages: 113-118

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: implication of different focal adhesion formation.2006

    • Author(s)
      Kamioka H , et. al.,
    • Journal Title

      Journal of Bone and Mineral Research 21

      Pages: 1012-1021

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] AlphaVbeta3 integrin ligands enhance volume-sensitive calcium influx in mechanically stretched osteocytes.2006

    • Author(s)
      Miyauchi A , et. al.,
    • Journal Title

      J Bone Miner Metab 24

      Pages: 498-504

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Roles of PKC, PI3K and JNK in multiple transduction of CCN2/CTGF signals in chondrocvtes2006

    • Author(s)
      Yosimichi G et. al.
    • Journal Title

      Bone 38

      Pages: 853-863

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increase of Galanin in Trigeminal Ganglion during Tooth Movement2006

    • Author(s)
      Deguchi T et. al.
    • Journal Title

      J Dent Res 85

      Pages: 658-663

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The presence of ABCG2-dependent side population cells in human periodontal ligaments2006

    • Author(s)
      Kawanabe N et. al.
    • Journal Title

      BBRC 344

      Pages: 1278-1283

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Brain-derived neurotrophic factor-immunoreactive primary sensory neurons in the rat trigeminal ganglion and trigeminal sensory nuclei2006

    • Author(s)
      Ichikawa H et. al.
    • Journal Title

      Brain Research 1081

      Pages: 113-118

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: implication of different focal adhesion formation2006

    • Author(s)
      Kamioka H et. al.
    • Journal Title

      J Bone Miner Res 21

      Pages: 1012-1021

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] AlphaVbeta3 integrin ligands enhance volume-sensitive calcium influx in mechanically stretched osteocytes2006

    • Author(s)
      Miyauchi A et. al.
    • Journal Title

      J Bone Miner Metab 24

      Pages: 498-504

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bone morphogenetic protein 3 expression pattern in rat condylar cartilage, femoral cartilage and mandibular fracture callus.2005

    • Author(s)
      Zheng L , et. al.,
    • Journal Title

      Eur J Oral Sci. 113

      Pages: 318-325

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Three-dimensional reconstruction of chick calvarial osteocytes and the ir cell processes using confocal microscopy.2005

    • Author(s)
      Sugawara Y.,
    • Journal Title

      Bone 36

      Pages: 877-883

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression of osteopontin mRNA in odontoclasts revealed by in situ hybridization during experimental tooth movement in mice.2005

    • Author(s)
      Kuroda S , et. al.,
    • Journal Title

      J Bone Miner Metab. 23

      Pages: 110-113

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Experimental tooth movement upregulates preproenkephalin mRNA in the rat trigeminal nucleus caudalis and oralis.2005

    • Author(s)
      Balam TA , et. al.,
    • Journal Title

      Brain Res 1036

      Pages: 196-201

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Bone morphogenetic protein 3 expression pattern in rat condylar cartilage, femoral cartilage and mandibular fracture callus2005

    • Author(s)
      Zheng L et. al.
    • Journal Title

      Eur J Oral Sci. 113

      Pages: 318-325

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Three-dimensional reconstruction of chic kcalvarial osteocytes and their cell processes using confocal microscopy2005

    • Author(s)
      Sugawara Y.
    • Journal Title

      Bone 36

      Pages: 877-883

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of osteopontin mRNA in odontoclasts revealed by in situ hybridization during experimental tooth movement in mice2005

    • Author(s)
      Kuroda S et. al.
    • Journal Title

      J Bone Miner Metab 23

      Pages: 110-113

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Experimental tooth movement upregulates preproenkephalin mRNA in the rat trigem inal nucleus caudalis and oralis2005

    • Author(s)
      Balam TA et. al.
    • Journal Title

      Brain Res. 1036

      Pages: 196-201

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 軟骨特異的にCCN2/CTGFを過剰発現したトランスジェニックマウスの作製と解析2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      日本分子生物学会 日本生化学会
    • Place of Presentation
      横浜市
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cartilage specific over expression of CCN2/CTGF transgenic mouse2007

    • Author(s)
      Tomita N ed. al.
    • Organizer
      Annual Meeting of Biochemistry and Molecular Biology and Japanese Biochemical Society
    • Place of Presentation
      Yokohama
    • Year and Date
      20071211-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 生骨組織中での細胞間コミュニケーションに影響する外的調節因子について2007

    • Author(s)
      石原嘉人, 他
    • Organizer
      日本矯正歯科学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 骨芽細胞から骨細胞への弾性の変化について2007

    • Author(s)
      菅原康代, 他
    • Organizer
      日本矯正歯科学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Runx2/Cbfal heterozygous knockout miceにおける歯の移動の遅延における分子生物学的研究2007

    • Author(s)
      玉村長都, 他
    • Organizer
      日本矯正歯科学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 下顎器官培養系におけるエレクトロポレーション法による遺伝子導入の応用2007

    • Author(s)
      寺尾文恵, 他
    • Organizer
      日本矯正歯科学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 骨芽細胞株MC3T3-E1細胞において流体剪断応力はMAPKを介してCTGFの発現を増大させる2007

    • Author(s)
      本城正, 他
    • Organizer
      日本矯正歯科学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cell-Cell Communication in Living Bone and Its Regulation Factor2007

    • Author(s)
      Ishihara Y ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Osaka
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The Elastic Modulus of Osteoblasts and Osteocytes2007

    • Author(s)
      Sugawara Y ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Osaka
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Molecular biological research of delayed tooth movement in Runx2/Cbfal heterozygous knock out mice2007

    • Author(s)
      Tamamura N ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Osaka
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Application of electroporation to mandibular explant culture system for gene transfection2007

    • Author(s)
      Terao F ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Osaka
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Fluid flow induces expression of connective tissue growth factor in MC3T3-E1 osteoblasts via MAPK pathway2007

    • Author(s)
      Honjo T ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Osaka
    • Year and Date
      20070919-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Cell-Cell Communication in Living Bone and Its Regulation Factor.2007

    • Author(s)
      Ishihara Y , et. al.,
    • Organizer
      American Society for Bone and Mineral Research
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      20070916-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The Elastic Modulus of Osteoblasts and Osteocytes.2007

    • Author(s)
      Sugawara Y , et. al.,
    • Organizer
      American Society for Bone and Mineral Research
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      20070916-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cell-Cell Communication in Living Bone and Its Regulation Factor2007

    • Author(s)
      Ishihara Y ed. al.
    • Organizer
      American Society for Bone and Mineral Research
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      20070916-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The Elastic Modulus of Osteoblasts and Osteocytes2007

    • Author(s)
      Sugawara Y ed. al.
    • Organizer
      American Society for Bone and Mineral Research
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      20070916-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 軟骨特異的CCN2/CTGF過剰発現トランスジェニックマウスの作製とCCN2/CTGFの内軟骨骨性骨化に及ぼす影響について2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      歯科基礎医学会学術大会
    • Place of Presentation
      札幌市
    • Year and Date
      20070830-31
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Influence and effects of CCN2/CTGF to cartilage formation in CCN2/CTGF over expression transgenic mouse2007

    • Author(s)
      Tomita N ed. al.
    • Organizer
      Japanese ASSociation of Oral Biology
    • Place of Presentation
      Sapporo
    • Year and Date
      20070830-31
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 骨細胞における機械的刺激への応答能-focal adhesionと細胞弾性率の関与2007

    • Author(s)
      山本照子, 他
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 超高電圧TEMトモグラフィーを用いた骨細胞周囲の高精細構造・応力解析2007

    • Author(s)
      上岡寛, 他
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 骨芽細胞から骨細胞への弾性の変化について2007

    • Author(s)
      菅原康代, 他
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 成長板軟骨におけるCCN4/WISP1 mRNA発現およびそのスプライシングバリアントの発現とその機能2007

    • Author(s)
      柳田剛志, 他
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 単離骨細胞の細胞突起/細胞体の力学的刺激に対するカルシウム応答の違い2007

    • Author(s)
      青沼有紀, 他
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] CCN2/CTGF過剰発現トランスジェニックマウスの作製によるCCN2/CTGFの内軟骨性骨化における役割解明2007

    • Author(s)
      冨田奈緒, 他
    • Organizer
      日本骨代謝学会
    • Place of Presentation
      大阪市
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Responce of osteoblast to mechanical stress- Focal adhesion formation and elastic modulus2007

    • Author(s)
      Takano-Yamamoto ed. al.
    • Organizer
      Japanese Society of Bone Metabolism
    • Place of Presentation
      Osaka
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Microscopic structure and stress analysis surrounding osteoblasts by TEM tomography2007

    • Author(s)
      Kamioka ed. al.
    • Organizer
      Japanese Society of Bone Metabolism
    • Place of Presentation
      Osaka
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The Elastic Modulus from Osteoblasts to Osteocytes2007

    • Author(s)
      Sugawara Y ed. al.
    • Organizer
      Japanese Society of Bone Metabolism
    • Place of Presentation
      Osaka
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Wnt-induced secreted protein 1 (WISP1/CCN4) mRNA splicing variants in normal and transformed chondrocytes2007

    • Author(s)
      Yanagita T ed. al.
    • Organizer
      Japanese Society of Bone Metabolism
    • Place of Presentation
      Osaka
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The processes of 3D-cultured MC3T3-E1 cells: Different in calcium response to mechanical stress2007

    • Author(s)
      Aonuma A ed. al.
    • Organizer
      Japanese Society of Bone Metabolism
    • Place of Presentation
      Osaka
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analysis of effects of CCN2/CTGF to cartilage formation in CCN2/CTGF transgenic mouse2007

    • Author(s)
      Tomita N ed. al.
    • Organizer
      Japanese Society of Bone Metabolism
    • Place of Presentation
      Osaka
    • Year and Date
      20070719-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Role of mechanical-stress inducibleprotein HCS24/CTGF/CCN2 in cartilagegrowth and regeneration.2007

    • Author(s)
      Takigawa M , et. al.,
    • Organizer
      International Symposium on Mechanobiology of Cartilage and Chondrocyte
    • Place of Presentation
      Greece, Athens
    • Year and Date
      20070510-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Role of mechanical-stress inducible protein HCS24/CTGF/CCN2 in cartilage growth and regeneration2007

    • Author(s)
      Takigawa M ed. al.
    • Organizer
      International Symposium on Mechanobiology of Cartilage and Chondrocyte
    • Place of Presentation
      Greece, Athens
    • Year and Date
      20070510-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] CTGF mRNA expression and apoptosis in osteocytes by mechanical stimulation.2007

    • Author(s)
      Sakai Y , et. al.,
    • Organizer
      International Association for Dental Research
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      20070321-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] CTGF mRNA expression and apoptosis in osteocytes by mechanical stimulation2007

    • Author(s)
      Sakai Y ed. al.
    • Organizer
      International Association for Dental Research
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      20070321-24
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 成長板軟骨細胞と軟骨肉腫細胞株におけるCCN4/WISP1 mRNAおよびそのスプライシングバリアントの発現とその機能2007

    • Author(s)
      柳田剛志, 他
    • Organizer
      第20回日本軟骨代謝学会
    • Place of Presentation
      岡山市
    • Year and Date
      20070302-03
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Wnt-induced secreted Protein 1 (WISP1/CCN4) mRNA splicing variants in normal and transformed chondrocytes2007

    • Author(s)
      Yanagita T ed. al.
    • Organizer
      Japanese Society of Cartilage Metabolism
    • Place of Presentation
      Okayama
    • Year and Date
      20070302-03
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Development of mechanical strain cell culture system for mechanobiological analysis.2007

    • Author(s)
      Masuda T , et. al.,
    • Organizer
      International Symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Less response of osteocytes than osteoblasts to mechanical force: Implication of different focal adhesion formation.2007

    • Author(s)
      Takano-Yamamoto T , et. al.,
    • Organizer
      International Symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Mechanical stretch inhibits chondrogenesis through ERK1/2 phosphorylation in micromass culture.2007

    • Author(s)
      Takahashi I , et. al.,
    • Organizer
      International Symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Application of electroporation to mandibular explant culture system for gene transfection.2007

    • Author(s)
      Terao F , et. al.,
    • Organizer
      International Symposium for Interface Oral Health Science in Sendai
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Development of mechanical strain cell culture system for mechanobiological analysis2007

    • Author(s)
      Masuda T ed. al.
    • Organizer
      International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Lessresponse of osteocytes than osteoblasts to mechanical force: Implication of different focal adhesion formation2007

    • Author(s)
      Takano-Yamamoto T ed. al.
    • Organizer
      Internation Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Mechanical stretch inhibits chodrogenesis through ERK1/2 phosphorylation in micromas culture2007

    • Author(s)
      Takahashi I ed. al.
    • Organizer
      International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Application of electroporation of mandibular explant culture system for gene transfection2007

    • Author(s)
      Terao F ed. al.
    • Organizer
      Internation Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Year and Date
      20070218-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes.2007

    • Author(s)
      Murshid SA , et. al.,
    • Organizer
      東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台市
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 軟骨細胞の機械的刺激応答機構におけるMAPKが果たす役割2007

    • Author(s)
      高橋一郎, 他
    • Organizer
      東北大学バイオサイエンスシンポジウム
    • Place of Presentation
      仙台市
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Actin and microtubule cytoskeletons of the processes of 3D-cultured MC3T3-E1 cells and osteocytes2007

    • Author(s)
      Murshid SA ed. al.
    • Organizer
      Tohoku University Bioscience Symposium
    • Place of Presentation
      Sendai
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Role of MAPK in mechanical stress response in chondrocytes2007

    • Author(s)
      Takahashi I ed. al.
    • Organizer
      Tohoku University Bioscience Symposium
    • Place of Presentation
      Sendai
    • Year and Date
      2007-06-04
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 歯根膜細胞による骨芽細胞転写因子の発現制御2007

    • Author(s)
      佐藤綾, 他
    • Organizer
      東北骨代謝研究会
    • Place of Presentation
      仙台市
    • Year and Date
      2007-02-03
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Inhibition of osteoblast transcription factor in periodontal ligament cells2007

    • Author(s)
      Sato A ed. al.
    • Organizer
      Tohoku Bone Metabolism
    • Place of Presentation
      Sendai
    • Year and Date
      2007-02-03
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Wnt-induced secreted protein 1 (WISP1/CCN4) mRNA splicing variants in normal and transformed chondrocytes2006

    • Author(s)
      Yanagita T , et. al.,
    • Organizer
      INTERNATIONAL WORKSHOP ON THE CCN FAMILY OF GENES
    • Place of Presentation
      Okayama, Japan
    • Year and Date
      20061118-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Wnt-induced secreted protein 1 (WISP1/CCN4) mRNA splicing variants in normal and transformed chondrocytes2006

    • Author(s)
      Yanagita T ed. al.
    • Organizer
      International Workshop on the CCN Family of Genes
    • Place of Presentation
      Okayama
    • Year and Date
      20061118-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Superior salivatory neurons receive the descending inhibitory synapticinputs2006

    • Author(s)
      Fujii A , et. al.,
    • Organizer
      日本矯正歯科学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 生理的歯の移動時のカルシトニン遺伝子関連ペプチドと破骨細胞および骨芽細胞との三次元組織学的形態計測2006

    • Author(s)
      藪内利憲, 他
    • Organizer
      日本矯正歯科学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] マウス実験的歯の移動における結合組織成長因子CTGFの発現とアポトーシスの誘導2006

    • Author(s)
      酒井雄一, 他
    • Organizer
      日本矯正歯科学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Increase of Galanin immunoreactive trigeminal ganglion cells during experimental tooth movement2006

    • Author(s)
      Deguchi T , et. al.,
    • Organizer
      日本矯正歯科学会大会
    • Place of Presentation
      札幌市
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Superior salivatory neurons receive the descending inhibitory synaptic inputs2006

    • Author(s)
      Fujii A ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Sapporo
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Three dimensional histomorphometric analysis of CGRP and Osteogenic cells during physiological tooth movement2006

    • Author(s)
      Yabuuchi ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Sapporo
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] CTGF mRNA expression and apoptosis in osteocytes by mechanical stimulation2006

    • Author(s)
      Sakai ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Sapporo
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Increase of Galanin immunoreactive trigeminal ganglion cells during experimental tooth movement2006

    • Author(s)
      Deguchi T ed. al.
    • Organizer
      Japanese Orthodontic Society
    • Place of Presentation
      Sapporo
    • Year and Date
      20060913-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Calcium responses of primary chick bone cells to fluid flow.2006

    • Author(s)
      Kamioka H , et. al.,
    • Organizer
      International. Association of Dental Research
    • Place of Presentation
      Brisben
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Three-dimensional quantitative analysis of CGRP during physiological tooth movement2006

    • Author(s)
      Yabuuchi T , et. al.,
    • Organizer
      International. Association of Dental Research
    • Place of Presentation
      Brisben
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Micro-elasticity of osteoblast and osteocyte-the implication of different focal adhesion formation2006

    • Author(s)
      Sugawara Y , et. al.,
    • Organizer
      International Association of Dental Research
    • Place of Presentation
      Brisben
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of gradually increasing force on Orthodontic Tooth Movement2006

    • Author(s)
      Urayama S , et. al.,
    • Organizer
      International Association of Dental Research
    • Place of Presentation
      Brisben
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Calcium responses of primary chick bone cells to fluid flow2006

    • Author(s)
      Kamioka H ed. al.
    • Organizer
      International Association of Dental Research
    • Place of Presentation
      Brisbane
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Three-dimensional quantitative analysis of CGRP during physiological tooth movement2006

    • Author(s)
      Yabuuchi T ed. al.
    • Organizer
      International Association of Dental Research
    • Place of Presentation
      Brisbane
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Micro-elasticity of osteoblast and osteocyte-the implication of different focal adhesion formation2006

    • Author(s)
      Sugawara Y ed. al.
    • Organizer
      International Association of Dental Research
    • Place of Presentation
      Brisbane
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of gradually increasing force on Orthodontic Tooth Movement2006

    • Author(s)
      Urayama S ed. al.
    • Organizer
      International Association of Dental Research
    • Place of Presentation
      Brisbane
    • Year and Date
      20060628-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Wnt-induced secreted protein 1 (WISP1/CCN4) mRNA splicing variants in normal and transformed chondrocytes2006

    • Author(s)
      Yanagita T , et. al.,
    • Organizer
      International Congress of Biochemistry and Molecular Biology and FAOBMB Congress
    • Place of Presentation
      Kyoto Japan
    • Year and Date
      20060618-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Descending inhibitory synaptic inputs to rat superior superior salivatory nucleus2006

    • Author(s)
      Fujii A , et. al.,
    • Organizer
      生理学会
    • Place of Presentation
      群馬県
    • Year and Date
      20060618-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Wnt-induced secreted protein 1 (WISP1/CCN4) mRNA splicing variants in normal and transformed chondrocytes2006

    • Author(s)
      Yanagita T ed. al.
    • Organizer
      International Congress of Biochemistry and Molecular Biology and FAOBMB Congress
    • Place of Presentation
      Kyoto
    • Year and Date
      20060618-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Descending inhibitory synaptic inputs to rat superior salivatory nucleus2006

    • Author(s)
      Fujii A ed. al.
    • Organizer
      Physiological Society of Japan
    • Place of Presentation
      Gunma
    • Year and Date
      20060618-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 成長板軟軟骨細胞と軟骨肉腫由来の軟骨細胞株におけるCCN4/WISP1 mRNAおよびそのスプライシングバリアントの発現2006

    • Author(s)
      柳田剛志, 他
    • Organizer
      日本軟骨代謝学会
    • Place of Presentation
      横浜市
    • Year and Date
      20060303-04
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Wnt-induced secreted protein 1 (WISP1/CCN4) mRNA splicing variants in normal and transformed chondrocytes2006

    • Author(s)
      Yanagita T ed. al.
    • Organizer
      Japanese Cartilage Metabolism Society
    • Place of Presentation
      Yokohama
    • Year and Date
      20060303-04
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ラット上唾液核細胞への抑制性シナプス入力に関する検討2006

    • Author(s)
      藤井昭仁, 他
    • Organizer
      唾液腺研究会
    • Place of Presentation
      愛知県
    • Year and Date
      20060224-25
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Superior salivatory neurons receive the descending inhibitory synaptic inputs2006

    • Author(s)
      Fujii A ed. al.
    • Organizer
      Salivary Gland Society
    • Place of Presentation
      Aichi
    • Year and Date
      20060224-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 生骨組織への力学刺激に対する骨細胞カルシウム応答のリアルタイム解析2006

    • Author(s)
      伊藤慎一, 他
    • Organizer
      富士裾野フォーラム(帝人)
    • Place of Presentation
      裾野市
    • Year and Date
      20060127-28
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Realtime amalysis of calcium response in osteoblast to mechanical stress2006

    • Author(s)
      Itoh S ed. al.
    • Organizer
      Fuji Susono Forum
    • Place of Presentation
      Susono
    • Year and Date
      20060127-28
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ニワトリ胚頭蓋冠培養組織内における骨綱胞の力学応答観察2006

    • Author(s)
      菅原康代, 他
    • Organizer
      日本機械学会・バイオエンジニアリング講演会
    • Place of Presentation
      新潟市
    • Year and Date
      20060113-14
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Response of mechanical stress to Osteocytes in cultured chick calvaria2006

    • Author(s)
      Sugawara Y ed. al.
    • Organizer
      Japanese Mechanical and Engineering Symposium
    • Place of Presentation
      Niigata
    • Year and Date
      20060113-14
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 培養ヒト歯根膜細胞におけるSide Population細胞の存在について2006

    • Author(s)
      川邉紀章, 他
    • Organizer
      岡山歯学会総会
    • Place of Presentation
      岡山市
    • Year and Date
      2006-11-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Side population of cultured human periodontal ligament cells2006

    • Author(s)
      Kawanabe H ed. al.
    • Organizer
      Okayama Dental Association
    • Place of Presentation
      Okayama
    • Year and Date
      2006-11-12
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Observation of Calcium Response of isolated osteocyte to Localized Mechanical Stimulus2005

    • Author(s)
      Tanaka M , et. al.,
    • Organizer
      International Conference on Biomedical Engineering
    • Place of Presentation
      シンガポール
    • Year and Date
      20051207-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Observation of Calcium Response of isolated osteocyte to Localized Mechanical Stimulus2005

    • Author(s)
      Tanaka M ed. al.
    • Organizer
      International Conference on Biomedical Engineering
    • Place of Presentation
      Singapore
    • Year and Date
      20051207-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bone Morphogenetic Protein 3 (BMP3) Expression Pattern in Rat Condylar Cartilage, Femoral Cartilage and Mandibular Fracture Callus2005

    • Author(s)
      Zheng L , et. al.,
    • Organizer
      日中医学大会
    • Place of Presentation
      上海
    • Year and Date
      20051111-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Bone Morphogenetic Protein 3 (BMP3) expression pattern in rat condylar cartilage, femoral cartilage and mandibular fracture callus2005

    • Author(s)
      Zheng L ed. al.
    • Organizer
      Sino-Japanese Conference on Stomatology
    • Place of Presentation
      Shanghai
    • Year and Date
      20051111-13
    • Description
      「研究成果報告書概要(欧文)」より
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    • Organizer
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    • Description
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Published: 2010-02-04  

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