アジアにおけるエキノコックス、テニア条虫の種分化、病原性分化、分子進化調査研究

2008 Fiscal Year Annual Research Report

• Project/Area Number: 17256002
• Research Institution: Asahikawa Medical College
• Principal Investigator: 伊藤 亮 Asahikawa Medical College, 医学部, 教授 (70054020)
• Co-Investigator(Kenkyū-buntansha): 中尾 稔 旭川医科大学, 医学部, 准教授 (70155670) ; 中谷 和宏 旭川医科大学, 医学部, 准教授 (70109388) ; 迫 康仁 旭川医科大学, 医学部, 助教 (40312459) ; 柳田 哲矢 旭川医科大学, 医学部, 助教 (40431837)
• Keywords: エキノコックス条虫 / 多包条虫 / 単包条虫 / テニア条虫 / 有鉤条虫 / 無鉤条虫 / 遺伝子多型 / 分子分類学

Research Abstract

代表的な成果について概説する。1) エキノコックス条虫関係の成果 : 地球規模でのエキノコックス条虫の遺伝子多型についてミトコンドリア伝子解析を行い、a) 北半球に分布している多包条虫(E. multilocularis)は3系統の遺伝子系に大別できることを報告した(Nakao et.al. submitted)、b) 中国大陸における3種類(E. granulosus, E. Multilocularis, E. shiquicus)のうち遺伝子多型が認められるのはE. shiquicusだけであること、他の2種は小さな集団として中国国内で分布域を拡大したと考えられた(Li et.al. Trans R Soc Trop Med Hyg 2008, 102 ; Nakao et.al. in prep.)、さらにc) ペルー、ヨーロッパにおける単包条虫のミトコンドリアならびに核遺伝子を解析し、これまでG1と呼ばれている単包条虫集団内での遺伝子多型は非常に限定されていることが判明した(Snabel et.al. Parasitol Res 2009, in press ; Pedro et.al. 2009, Parasltol Int in press)。またd) これまで症例の確認ができていなかったモンゴル、イランの多包虫症症例を確認できた(Ito et.al. in prep他)。2) テニア条虫関係の成果 : e) 新しい簡便な遺伝子解析法を開発し(Nkouawa et.al. J Clin Microbiol 2009, 47)、アジア各国の患者糞便を用いる解析を実施している。f) タイで入手したテニア条虫2個体のミトコンドリアならびに核遺伝子の解析から、雑種個体と結論付けられた(Okamoto et.al. in prep.)。g) インドネシアにおける嚢虫症患者の病巣を用いる遺伝子解析結果からイリアンジャヤを含む他のアジア地域と遺伝子型が異なることが示唆された(Sudewi et.al. Trans R Soc Trop Med Hyg 2008, 102)。3) 裂頭条虫に関する成果 : 日本海裂頭条虫(Dipphylobothrium nihonkaiense)とヒグマ裂頭条虫(D. ursi)との分類学的考察を加えた(Yanagida et.al.in prep.)。すべての寄生虫種において地球規模での解析が必要かつ可能であることが判明してきた。

Research Products

• [Journal Article] Molecular identification of Echinococcus granulosus fromPer (2009)
  • Author(s): Moro P, Nakao M, Ito A, Schantz PM, Cavero C, Cabrera L
  • Journal Title: Parasitology International 58(In press)
  • Peer Reviewed
• [Journal Article] Cystic echinococcosis in Turkey : genetic variability and first record of the pig strain (G7) in the country (2009)
  • Author(s): Snavel V, Altintas N, D'Amelio S, Nakao M, Romig T, Yolasigmaz A, Gunes K, Turk M, Busi M, Yuttner M, Sevcova D, Ito A, Altintas N, Dubinsky P
  • Journal Title: Parasitology Research (In press)
  • Peer Reviewed
• [Journal Article] Close relationship between clinical regression and specific serology in the follow-up of patients with alveolar echino-coccosis in different clinical stages (2009)
  • Author(s): Tappe D, Frosch M, Sako Y, Itoh S, Gruner B, Reuter S, Nakao M, Ito A, Kern P
  • Journal Title: American Journal of Tropical Medicine and Hygiene 80(In press)
  • Peer Reviewed
• [Journal Article] Paragdnimiasis in Cameroon : molecular identification, seroaiagnosis and clinical manifestations (2009)
  • Author(s): Nkouawa A, Okamoto M, Mabou AK, Edinga E, Yamasaki H, Sako Y, Nakao M, Nakaya K, Blair D, Agatsuma T, Enyong P, Shibahara T, Moyou-Somo R, Ito A
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 103 Pages: 255-261
  • Peer Reviewed
• [Journal Article] Development of an immunochromatographic test to detectantibodies against_recombinant Em18 for diagnosis of alveolar echinococcosis (2009)
  • Author(s): Sako Y, Fukuda K, Kobayashi Y, Ito A
  • Journal Title: Journal of Clinical Microbiology 47 Pages: 252-254
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species (2009)
  • Author(s): Nkouawa A, Sako Y, Nakao M, Nakaya K, Ito A
  • Journal Title: Journal of Clinical Microbiology 47 Pages: 168-174
  • Peer Reviewed
• [Journal Article] Small mammal assemblages and habitat distribution in thenorthern Junggar basin, Xinjiang, China : a pilot survey (2008)
  • Author(s): Giraudoux P, Zhou H, Quere JP, Raoul F, Delattre P, Voloboouev V, Deforet T, Ito. A, Mamuti W, Scheifler R, Craig PS
  • Journal Title: Mammalia 72 Pages: 309-319
  • Peer Reviewed
• [Journal Article] Molecular and immunological diagnosis of taeniasis and cysticercosis in Asia and the Pacific (2008)
  • Author(s): Ito A, Sako Y, Nakao M, Nakaya K, Okamoto M, Wandra T, Kandun IN, Anantaphruti MT, Waikagul J, Li TY, Qiu DC
  • Journal Title: Southeast Asian Journal of Tropical Medicine and Public Health 39Suppl 1 Pages: 37-47
  • Peer Reviewed
• [Journal Article] Echinococcoses and Tibetan communities (2008)
  • Author(s): Craig PS, Li TY, Qiu JM, Zhen R, Wang Q, Giraudoux P, Ito A, Heath D, Warnock B, Schantz P, Yang W
  • Journal Title: Emerging Infectious Diseases 14 Pages: 1674-1675
  • Peer Reviewed
• [Journal Article] Genetic analysis of Echinococcus multilocularis originating from a patient with alveolar echinococcosis occurring in Minnesota in 1977 (2008)
  • Author(s): Yamasaki. H, Nakao M, Nakaya K, Schantz PM, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 79 Pages: 245-247
  • Peer Reviewed
• [Journal Article] Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda : Taeniidae) from the African lion (2008)
  • Author(s): Huttner M, Nakao M, Wassermann T, Siefert L, Boomker. JDF, Dinkel A, Sako Y, Mackenstedt U, Romig T, Ito A
  • Journal Title: International Journal for Parasitology 38 Pages: 861-868
  • Peer Reviewed
• [Journal Article] Species identification of human echinococcosis using histo pathology and genotyping in northwestern China (2008)
  • Author(s): Li TY, Ito A, Nakaya K, Qiu JM, Nakao M, Zhen R, Xiao N, Chen XW, Giraudoux P, Craig PS
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 102 Pages: 585-590
  • Peer Reviewed
• [Journal Article] Evaluation of three PCR assays for the identification ofthe sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues (2008)
  • Author(s): Boufana BS, Campos-Ponce M, Naidich A, Buishi I, Lahmar S, Zeyhle E, Jenkins DJ, Combes B, Wen H, Xiao N, Nakao M, Ito A, Qiu JM, Craig PS
  • Journal Title: American Journal of Tropical Medicine and Hygiene 78 Pages: 777-783
  • Peer Reviewed
• [Journal Article] Taenia solium cysticercosis in Bali, Indonesia : serology and mtDNA analysis (2008)
  • Author(s): Sudewi AAR, Wandra T, Artha A, Nkouawa A, Ito A
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 102 Pages: 96-98
  • Peer Reviewed
• [Journal Article] 医学と医療の最前線エキノコックス症に関する診断法の進展 (2008)
  • Author(s): 伊藤亮、中尾稔、迫康仁、中谷和宏、石川裕司、柳田哲矢
  • Journal Title: 日本内科学会雑誌 97 Pages: 1900-1909
• [Presentation] Topics on cestode zoonosis : Echinococcus and echinococcosis (2009)
  • Author(s): Ito A
  • Organizer: 43^<rd> US-Japan Joint Conference on Parasitic Diseases
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-01-07
• [Presentation] Asia-Africa scientific platform project sponsored by the Japan Society forthe Promotion of Science (JSPS) (2008)
  • Author(s): Ito A
  • Organizer: Joint International Tropical Medicine Meeting 2008
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2008-10-13
• [Presentation] Key note speech on Echinococcosis : Serological detection of patients and animals (2008)
  • Author(s): Ito A
  • Organizer: 17th International Congress for Tropical Medicine and Malaria
  • Place of Presentation: Cheju, Korea
  • Year and Date: 2008-09-30
• [Presentation] Echinococcosis : Serodiagnosis for detection of patients and molecularidentification of parasites (2008)
  • Author(s): Ito A
  • Organizer: 10^<th> European Multi-colloquium of Parasitology
  • Place of Presentation: Paris, France
  • Year and Date: 2008-08-25
• [Book] Handbook of Nucleic Acid Purification (ed. by Liu D) Chapter 13. Purification of nucleic acids from helminthes. 271-291 (2009)
  • Author(s): Okamoto M, Ito A
  • Publisher: CRC Press, Taylor & Francis Group(In press)
• [Book] Food-born Pathogens (ed. by Liu D) Chapter 62. Taeniasis. 827-838 (2009)
  • Author(s): Ito A, Nakao M, Sako Y, Nakaya K, Yanagida T, Okamoto M
  • Publisher: CRC Press, Taylor & Francis Group(In press)
• [Book] Clinical Neurocysticercosis Immunodiagnosis and molecular diagnosis for cysticercosis: usefulness of cerebrospiral fluid (CSF) and serum samples (2009)
  • Author(s): Ito A, Takayanagui OM
  • Publisher: The World Federation of Neurocvsticercosis(In press)
• [Book] Zoonoses Taenia solium, Taenia saginata and Taenia asiatica (2009)
  • Author(s): Flisser A, Craig PS, Ito A
  • Publisher: Oxford University Press(In press)