Speciation, pathogenicity and evolution of Echinococcus and Taenia tapeworms in Asia

2008 Fiscal Year Final Research Report

• Project/Area Number: 17256002
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Asahikawa Medical College
• Principal Investigator: ITO Akira Asahikawa Medical College, 医学部, 教授 (70054020)
• Co-Investigator(Kenkyū-buntansha): NAKAO Minoru 旭川医科大学, 医学部, 准教授 (70155670) ; NAKAYA Kazuhiro 旭川医科大学, 医学部, 准教授 (70109388) ; SAKO Yasuhito 旭川医科大学, 医学部, 助教 (40312459) ; YANAGIDA Tetsuya 旭川医科大学, 医学部, 助教 (40431837) ; OKAMOTO Munenori 鳥取大学, 農学部, 准教授 (70177096)
• Project Period (FY): 2005 – 2008
• Keywords: 地球規模寄生虫感染症 / 人獣共通寄生虫疾患 / エキノコックス症 / 脳嚢虫症

Research Abstract

エキノコックス症、脳嚢虫症は新興・再興寄生虫感染症として国際的な問題になっている。本研究ではアジアにおけるこれらの寄生虫の多様性(種、病原性)ならびに地理拡散について、進化学的な観点から調査研究を展開する。条虫の分類学的再検討を含め、種間変異、種内変異の解析を中心に、それぞれの種の多様性の解析を試み、(1)遺伝子ならびに形態学的解析に基づく分類学的再検討、(2)病原性の多様性、(3)条虫症の地球上での分布拡散、(4)進化学的考察についての解析・考察を試みる。

Research Products

• [Journal Article] Kern P. Close relationship between clinical regression and specific serology in the follow-up of patients with alveolar echinococcosis in different clinical stages (2009)
  • Author(s): Tappe D, Frosch M, Sako Y, Itoh S, Gruner B, Reuter S, Nakao M, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 80 Pages: 792-797
  • Peer Reviewed
• [Journal Article] Schantz PM, Cavero C, Cabrera L. Molecular identification of Echinococcus isolates from Peru (2009)
  • Author(s): Moro PL, Nakao M, Ito A
  • Journal Title: Parasitology International 58 Pages: 184-186
  • Peer Reviewed
• [Journal Article] Genetic analysis of Echinococcus multilocularisoriginating from a patient with alveolar echinococcosis occurring in Minnesota in 1977 (2008)
  • Author(s): Yamasaki H, Nakao M, Nakaya K, Schantz PM, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 79 Pages: 245-247
  • Peer Reviewed
• [Journal Article] Scheifler R, Craig PS. Small mammal assemblages and habitat distribution in the northern Junggar Basin, Xinjiang China : a pilot survey. (2008)
  • Author(s): Giraudoux P, Zhou H, Quere JP, Raoul F, Delattre P, Volobouev V, Deforet T, Ito A, Mamuti W
  • Journal Title: Mammalia 72 Pages: 309-319
  • Peer Reviewed
• [Journal Article] Species identification of human echinococcosis using histopathology and genotyping in northwestern China (2008)
  • Author(s): Li T, Ito A, Nakaya K, Qiu JM, Nakao M, Zhen R, Xiao N, Chen XW, Giraudoux P, Craig PS
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 102 Pages: 585-590
  • Peer Reviewed
• [Journal Article] Qiu J, Craig PS. Evaluation of three PCR assays for the identification of the sheep strain (genotype 1) of Echinococcus granulosus in canid feces and parasite tissues (2008)
  • Author(s): Boufana BS, Campos-Ponce M, Naidich A, Buishi I, Lahmar S, Zeyhle E, Jenkins DJ, Combes B, Wen H, Xiao N, Nakao M, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 78 Pages: 777-783
  • Peer Reviewed
• [Journal Article] Echinococcoses and Tibetan communities (2008)
  • Author(s): Craig PS, Li T, Qiu J, Zhen R, Wang Q, Giraudoux P, Ito A, Heath D, Warnock B, Schantz P, Yang W
  • Journal Title: Emerging Infectious Diseases 14 Pages: 1674-1675
  • Peer Reviewed
• [Journal Article] Molecular and immunological diagnosis of taeniasis and cysticercosis in Asia and the Pacific (2008)
  • Author(s): Ito A, Sako Y, Nakao M, Nakaya K, Okamoto M, Wandra T, Kandun IN, Anantaphruti MT, Waikagul J, Li T, Qiu D
  • Journal Title: Southeast Asian Journal of Tropical Medicine and Public Health 39(Suppl 1) Pages: 37-46
  • Peer Reviewed
• [Journal Article] Genetic characterization and phylogenetic position of Echinococcus felidis Ortlepp, 1937 (Cestoda : Taeniidae) from the African lion (2008)
  • Author(s): Huttner M, Nakao M, Wassermann T, Siefert L, Boomker DF, Dinkel A, Sako Y, Mackenstedt U, Romig T, Ito A
  • Journal Title: International Journal for Parasitology 38 Pages: 861-868
  • Peer Reviewed
• [Journal Article] 医学と医療の最前線 エキノコックス症に関する診断法の進展 (2008)
  • Author(s): 伊藤亮, 中尾稔, 迫康仁, 中谷和宏, 石川裕司, 柳田哲矢
  • Journal Title: 日本内科学会雑誌 97 Pages: 1900-1909
  • Peer Reviewed
• [Journal Article] Molecular characterization of a novel gene encoding an 8-kDa-subunit of antigen B from Echinococcus granulosus genotypes 1 and 6 (2007)
  • Author(s): Mamuti W, Sako Y, Bart JM, Nakao M, Ma X, Wen H, Ito A
  • Journal Title: Parasitology International 56 Pages: 313-316
  • Peer Reviewed
• [Journal Article] Cloning and characterization of cathepsin L-like peptidases of Echinococcus multilocularis metacestodes (2007)
  • Author(s): Sako Y, Yamasaki H, Nakaya K, Nakao M, Ito A
  • Journal Title: Molecular and Biochemical Parasitology 154 Pages: 81-189
  • Peer Reviewed
• [Journal Article] A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes (2007)
  • Author(s): Nakao M, McManus DP, Schantz PM, Craig PS, Ito A
  • Journal Title: Parasitolog 134 Pages: 13-722
  • Peer Reviewed
• [Journal Article] Echinococcosis : serological detection of patients and molecular identification of parasites (2007)
  • Author(s): Ito A, Nakao M, Sako Y
  • Journal Title: Future Microbiology 2 Pages: 439-449
  • Peer Reviewed
• [Journal Article] Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand (2007)
  • Author(s): Anantaphruti MT, Yamasaki H, Nakao M, Waikagul J, Wattanakulpanich D, Nuamtanong S, Maipanich W, Pubampen S, Sanguankiat S, Muennoo C, Nakaya K, Sato MO, Sako Y, Okamoto M, Ito A
  • Journal Title: Emerging Infectious Diseases 13 Pages: 1413-1416
  • Peer Reviewed
• [Journal Article] Comparison of several commercial serologic kits and Em18 serology for detection of human alveolar echinococcosis (2007)
  • Author(s): Bart JM, Piarroux M, Sako Y, Grenouillet F, Bresson-Hadni S, Piarroux R, Ito A
  • Journal Title: Diagnostic Microbiology and Infectious Diseases 59 Pages: 93-95
  • Peer Reviewed
• [Journal Article] Community surveys and risk factor analysis of human alveolar and cystic echinococcosis in Ningxia Hui autonomous region China. (2007)
  • Author(s): Yang YR, Sun T, Li Z, Zhang J, Teng J, Liu X, Liu R, Zhao R, Jones MK, Wang Y, Wen H, Feng X, Zhao Q, Zhao Y, Shi D, Bartholomot B, Vuitton DA, Pleydell D, Giraudoux P, Ito A, Danson MF, Boufana B, Craig PS, Williams GM, McManus DP
  • Journal Title: Bulletin of the World Health Organization 84 Pages: 714-721
  • Peer Reviewed
• [Journal Article] A correlative study of ultrasound with serology in an area in China co-endemic for human alveolar and cystic echinococcosis (2007)
  • Author(s): Yang YR, Craig PS, Ito A, Vuitton DA, Giraudoux P, Sun T, Williams GM, Huang Z, Li Z, Wang Y, Teng J, Li Y, Huang L, Wen H, Jones MK, McManus DP
  • Journal Title: Tropical Medicine and International Health 12 Pages: 637-646
  • Peer Reviewed
• [Journal Article] Taeniasis in Mongolia, 2002-2006 (2007)
  • Author(s): Myadagsuren N, Davaajav A, Wandra T, Sandar T, Ichikhorloo P, Yamasaki H, Sako Y, Nakao M, Sato MO, Nakaya K, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 77 Pages: 342-346
  • Peer Reviewed
• [Journal Article] Assessment of use of microsatellite polymorphism analysis for improving spatial distribution tracking of Echinococcus multilocularis (2007)
  • Author(s): Knapp J, Bart JM, Glowatzki ML, Ito A, Gerard S, Maillard S, Piarroux R, Gottstein B.
  • Journal Title: Journal of Clinical Microbiology 45 Pages: 2943-2950
  • Peer Reviewed
• [Journal Article] Mitochondrial genomes of the human broad tapeworms Diphyllobothrium latum and Diphyllobothrium nihonkaiense (Cestoda : Diphyllobothriidae) (2007)
  • Author(s): Nakao M, Abmed D, Yamasaki H, Ito A
  • Journal Title: Parasitology Research 101 Pages: 233-236
  • Peer Reviewed
• [Journal Article] Taeniasis/cysticercosis in a Tibetan population in Sichuan province (2006)
  • Author(s): Li T, Craig PS, Ito A, Chen X, Qiu D, Qiu J, Sato MO, Wandra T, Bradshaw H, Li L, Yang Y, Wang Q
  • Journal Title: China. Acta Tropica 100 Pages: 223-231
  • Peer Reviewed
• [Journal Article] Dual infection of animal hosts with different Echinococcus species in the eastern Qinghai-Tibet plateau region of China (2006)
  • Author(s): Xiao N, Nakao M, Qiu J, Budke CM, Giraudoux P, Craig PS, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 75 Pages: 292-294
  • Peer Reviewed
• [Journal Article] Evaluation of purified Taenia solium glycoproteins and recombinant antigens in the serologic detection of human and swine cysticercosis (2006)
  • Author(s): Sato MO, Sako Y, Nakao M, Yamasaki H, Nakaya K, Ito A
  • Journal Title: Journal of Infectious Diseases 194 Pages: 1783-1790
  • Peer Reviewed
• [Journal Article] Geno- typin g of human cystic echinococcosis in Xinjiang (2006)
  • Author(s): Bart JM, Abdukader M, Zhang YL, Lin RY,Wang YH, Nakao M, Ito A, Craig PS, Piarroux R, Vuitton DA, Wen H
  • Journal Title: PR China. Parasitology 133 Pages: 571-579
  • Peer Reviewed
• [Journal Article] Unique family clustering of human echinococcosis case in a Chinese community (2006)
  • Author(s): Yang YR, Elis M, Sun T, Li Z, Liu X, Vuitton DA, Bartholomot B, Giraudoux P, Craig PS, Boufana B, Wang Y, Feng Z, Wen H, Ito A, McManus DP
  • Journal Title: American Journal of Tropical Medicine and Hygiene 74 Pages: 487-494
  • Peer Reviewed
• [Journal Article] Towards the international collaboration for detection, surveillance and control of taeniasis/ cysticercosis and echinococcosis in Asia and the Pacific (2006)
  • Author(s): Ito A, Wandra T, Sato MO, Mamuti W, Xiao N, Sako Y, Nakao M, Yamasaki H, Nakaya K, Okamoto M, Craig PS.
  • Journal Title: Southeast Asian Journal of Tropical Medicine and Public Health 37 (Suppl 3) Pages: 82-90
  • Peer Reviewed
• [Journal Article] Neurocysticercosis : clinical manifestation, neuroimaging, serology and molecular confirmation of histopathologic specimens (2006)
  • Author(s): Ito A, Takayanagui OM, SakoY, Sato MO, Odashima NS, Yamasaki H, Nakaya K, Nakao M
  • Journal Title: Southeast Asian Journal of Tropical Medicine and Public Health 37 (Suppl 3) Pages: 74-81
  • Peer Reviewed
• [Journal Article] Simultaneous alveolar and cystic echinococcosis of the liver (2006)
  • Author(s): Yang YR, Liu XZ, Vuitton DA, Bartholomot B, Wang YH, Ito A, Craig PS, McManus DP
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 100 Pages: 597-600
  • Peer Reviewed
• [Journal Article] Evidence and potential for transmission of human and swine Taenia solium cysticercosis in the Piracuruca region, Piaui, Brazil (2006)
  • Author(s): Sato MO, Cavalcante V, Sako Y, Nakao M, Yamasaki H, Yatsuda P, Nakaya K, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 75 Pages: 933-935
  • Peer Reviewed
• [Journal Article] A case of intramuscular cysticercosis diagnosed definitively by mitochondrial DNA analysis of extremely calcified cysts (2006)
  • Author(s): Yamasaki H, Nagase T, Kiyoshige Y, Suzuki M, Nakaya K, Itoh Y, Sako Y, Nakao M, Ito A
  • Journal Title: Parasitology International 55 Pages: 127-130
  • Peer Reviewed
• [Journal Article] Echinococcus multilocularis: developmental stage-specific expression of antigen B 8-kDa-subunits (2006)
  • Author(s): Mamuti W, Sako Y, Xiao N, Nakaya K, Nakao M, Yamasaki H, Lightowlers MW,Craig PS, Ito A
  • Journal Title: Experimental Parasitology 113 Pages: 75-82
  • Peer Reviewed
• [Journal Article] Recent advances in characterization of Echinococcus antigen B (2006)
  • Author(s): Mamuti W, Sako Y, Nakao M, Xiao N, Nakaya K, Ishikawa Y, Yamasaki H, Lightowlers MS, Ito A
  • Journal Title: Parasitology International 55 Pages: S57-S62
  • Peer Reviewed
• [Journal Article] Mitochondrial DNA diagnosis for taeniasis and cysticercosis (2006)
  • Author(s): Yamasaki H, Nakao M, Sako Y, Nakaya K, Sato MO, Ito A
  • Journal Title: Parasitology International 55 Pages: S81-S85
  • Peer Reviewed
• [Journal Article] Usefulness of severe combined immunodeficiency (scid) and inbred mice for studies of cysticercosis and echinococcosis (2006)
  • Author(s): Nakaya K, Mamuti W, Xiao N, Sato MO, Wandra T, Nakao M, Sako Y, Yamasaki H, Ishikawa Y, Craig PS, Schantz PM, Ito A
  • Journal Title: Parasitology International 55 Pages: S91-S97
  • Peer Reviewed
• [Journal Article] Taeniasis/cysticercosis in Papua (Irian Jaya), Indonesia (2006)
  • Author(s): Margono SS, Wandra T, Swasono MF, Murni S, Craig PS, Ito A
  • Journal Title: Parasitology International 55 Pages: S143-S148
  • Peer Reviewed
• [Journal Article] Genetic variation in Taenia solium (2006)
  • Author(s): Campbell G, Garcia HH, Nakao M, Ito A, Craig PS.
  • Journal Title: Parasitology International 55 Pages: S121-S126
  • Peer Reviewed
• [Journal Article] Recombinant antigens for serodiagnosis of cysticercosis and echinococcosis (2006)
  • Author(s): Sako Y, Nakao M, Nakaya K, Yamasaki H, Ito A
  • Journal Title: Parasitology International 55 Pages: S69-S73
  • Peer Reviewed
• [Journal Article] The Echinococcosis working group in China (2006)
  • Author(s): Craig PS
  • Journal Title: Parasitology International 55 Pages: S221-S225
  • Peer Reviewed
• [Journal Article] Echinococcus shiquicus, a new species from the Qinghai-Tibet plateau region of China: discovery and epidemiological implications (2006)
  • Author(s): Xiao N, Qiu J, Nakao M, Li T, Yang W, Chen X, Schantz PM, Craig PS, Ito A
  • Journal Title: Parasitology International 55 Pages: S233-S236
  • Peer Reviewed
• [Journal Article] High prevalence of Taenia saginata taeniasis and status of Taenia solium cysticercosis in Bali, Indonesia, 2002-2004 (2006)
  • Author(s): Wandra T, Susisna P, Dharmawan NS, Margono SS, Sudewi R, Suroso T, CraigPS, Ito A
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 100 Pages: 346-353
  • Peer Reviewed
• [Journal Article] Conservation of the vaccine antigen gene, TSOL18, among genetically variant isolates of Taenia solium (2006)
  • Author(s): Gauci CG, Ito A, Lightowlers MW
  • Journal Title: Molecular and Biochemical Parasitology 146 Pages: 101-104
  • Peer Reviewed
• [Journal Article] Magnetic resonance imaging of alveolar echinococcosis experimentally induced in the rat lung (2006)
  • Author(s): Asanuma T, Kawahara T, Inanami O, Nakao M, Nakaya K, Ito ATakiguchi M, Hashimoto A, Kuwabara M
  • Journal Title: Journal of Veterinary Medical Science 68 Pages: 15-20
  • Peer Reviewed
• [Journal Article] Echinococcosis in Tibetan populations, western Sichuan province, China (2006)
  • Author(s): Li T, Qiu J, Yang W, Craig PS, Chen X, Xiao N, Ito A, Giraudoux P, Mamuti W, Yu W, Schantz PM.
  • Journal Title: Emerging Infectious Diseaess 11 Pages: 1866-1873
  • Peer Reviewed
• [Journal Article] evaluation of clinical and serological data from Taenia solium cysticercosis patients in eastern Inner Mongolia autonomous region, China (2006)
  • Author(s): Ikejima T, Piao ZX, Sako Y, Sato MO, Bao S, Si R, Yu F, Zhang CL, Nakao M, Yamasaki H, Nakaya K, Kanazawa T, Ito A
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 99 Pages: 625-630
  • Peer Reviewed
• [Journal Article] Echinococcus muiltilocularis confirmed on Kunashiri island, 15 kilometers from the eastern part of Hokkaido, Japan (2006)
  • Author(s): Sato M, Nakaya K, Nakao M, Xiao N, Yamasaki H, Sako Y, Naitoh Y, Kondo S, Kobayashi M, Ohtaishi N, Ito A
  • Journal Title: American Journal of Tropical Medicine and Hygiene 72 Pages: 284-288
  • Peer Reviewed
• [Journal Article] Echinococcus shiquicus n. sp., a taeniid cestode from Tibetan fox and plateau pika in China (2006)
  • Author(s): Xiao N, Qiu J, Nakao M, Li T, Yang W, Chen X, Schantz PM, Craig PS, Ito A
  • Journal Title: International Journal for Parasitology 35 Pages: 693-701
  • Peer Reviewed
• [Journal Article] Usefulness of recombinant Em18-ELISA to evaluate efficacy of treatment inpatients with alveolar echinococcosis (2006)
  • Author(s): Fujimoto Y, Ito A, Ishikawa Y, Inoue M, Suzuki Y, Ohhira M, Ohtake T, Kohgo Y
  • Journal Title: Journal of Gastroenterology 40 Pages: 426-431
  • Peer Reviewed
• [Presentation] Asia-Africa science platform project sponsored by the Japan Society for the Promotion of Science (JSPS) (2008)
  • Author(s): Ito A
  • Organizer: Joint International Tropical Medicine Meeting 2008
  • Place of Presentation: Bangkok, Thailand.
  • Year and Date: 2008-10-13
• [Presentation] Key note speech on Echinococcosis : Serological detection of patients and animals (2008)
  • Author(s): Ito A
  • Organizer: 17th International Congress for Tropical Medicine and Malaria
  • Place of Presentation: Cheju, Korea.
  • Year and Date: 2008-10-10
• [Presentation] Human echinococcosis on Eastan Tibetan plateau, China (2008)
  • Author(s): Ito A.
  • Organizer: 17th International Congress for Tropical Medicine and Malaria
  • Place of Presentation: Cheju, Korea.
  • Year and Date: 2008-10-10
• [Presentation] Human echinococcosis in Qinghai, China (2008)
  • Author(s): Ito A
  • Organizer: 17th International Congress for Tropical Medicine and Malaria
  • Place of Presentation: Cheju, Korea.
  • Year and Date: 2008-10-01
• [Presentation] Echinococcosis : Serodiagnosis for detection of patients and molecular identification of parasites (2008)
  • Author(s): Ito A
  • Organizer: 10th European Multi-colloquium of Parasitology
  • Place of Presentation: Paris, France
  • Year and Date: 2008-08-25
• [Presentation] Molecular and serological diagnosis of taeniasis and cysticercosis in Asia and the Pacific (2007)
  • Author(s): Ito A
  • Organizer: Joint International Tropical Medicine Meeting 2007
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2007-11-30
• [Presentation] Key note speech on Developmental stage- specific gene expression in Echinococcus multilocularis and E. granulosus (2007)
  • Author(s): Ito A
  • Organizer: 22nd International Congress of Hydatidology
  • Place of Presentation: Athens, Greece
  • Year and Date: 2007-05-16
• [Presentation] Keynote speech on CestodeBiology, ICOPA XI (2006)
  • Author(s): Ito A
  • Organizer: ICOPA XI
  • Place of Presentation: Glasgow, UK
  • Year and Date: 20060806-11
• [Presentation] The present situation of taeniasis and cysticercosis in Asia and the Pacific (2006)
  • Author(s): Ito A
  • Organizer: Joint International Tropical Medicine Meeting 2006
  • Place of Presentation: Bangkok, Thailand.
  • Year and Date: 2006-12-01
• [Presentation] Serological and molecular tools for diagnosis. Symposium on Clinical management of Alveolar Echinococcosis (2006)
  • Author(s): Ito A
  • Organizer: 55th Annual Meeting of American Society of Tropical Medicine and Hygiene
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2006-11-15
• [Book] The World Federation of Neurocysticercosis. Clinical Neurocysticercosis: Immunodiagnosis and molecular diagnosis for cysticercosis (2009)
  • Author(s): Ito A, Takayanagui OM
  • Publisher: usefulness of cerebrospiral fluid (CSF) and serum samples(in press)
• [Book] Oxford University Press. Zoonoses : Taenia solium (2009)
  • Author(s): Flisser A, Craig PS, Ito A
  • Publisher: Taenia saginata and Taenia asiatica (in press)
• [Book] CRC Press, Taylor & Francis Group. Handbook of Nucleic Acid Purification (ed. Liu D) Chapter13 (2009)
  • Author(s): Okamoto M, Ito A
  • Total Pages: 271-291
  • Publisher: Purification of Nucleic Acids from Helminthes
• [Book] CRC Press, Taylor & Francis Group. Food-born Pathogens (ed. Liu D) (2009)
  • Author(s): Ito A, Nakao M, Sako Y, Nakaya K, Yanagida T, Okamoto M
  • Total Pages: 827-838
  • Publisher: Chapter 62. Taeniasis
• [Book] Blackwell Publishing (2007)
  • Author(s): Ito A, Baker DG
  • Total Pages: 37-42
  • Publisher: Parasites of Laboratory Animals