2007 Fiscal Year Final Research Report Summary
Development of artificial arrayed nose system with drug metabolizing enzymes
| Project/Area Number |
17300058
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Perception information processing/Intelligent robotics
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MITSUBAYASHI Kohji Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Professor (40307236)
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| Co-Investigator(Kenkyū-buntansha) |
OTSUKA Kimio Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Associate Prof. (00211798)
SAITO Hirokazu Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Assistant Prof. (00205668)
KUDO Hiroyuki Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Assistant Prof. (70329118)
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| Project Period (FY) |
2005 – 2007
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| Keywords | drug metabolizing enzyme / optical fiber / UV-LED / NADH / ethanol vapor / olfactory system / bio-sniffer / nitrogen compound |
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| Research Abstract |
A novel artificial arrayed nose system with drug metabolizing system was investigated with following steps,(a) optical fluorescent fiber with alcohol dehydrogenase for ethanol vapor,(b) biochemical sniffer with flavin containing monooxygenase as one of the drug metabolizing enzyme,(c) bio-sniffer chip with screen printed electrodes. The detailed results are described as follows.
(a) The fiber optic type biochemical gas-sensor(bio-sniffer) was constructed by using NAD+ dependent dehydrogenase enzyme and exciting light source of UV-LED and portable spectrometer: In this sensor system, the concentration of ethanol vapor could be successfully measured by detecting the fluorescent change in NADH as ADH (alcohol dehydrogenase) enzymatic product by the portable fiber optic fluorometric system. The fluorescent sniffer system could be applied to other sniffers for other smell chemicals (acetaldehyde, formaldehyde, lactate, pyruvate, succinate, etc) using their dehydrogenase enzymes. The thinner
… More
fiber would be suitable for constructing the arrayed nose system for multi analyte in the smell.
(b) Triethylamine (TEA) is a colorless volatile organic compounds with a strong fishy malodor, like ammonia, in the gas phase. A bioelectronic sniffer for triethylamine (TEA) was developed with a flavin-containing monooxygenase type 3 (FMO-3). The TEA biosniffer consisted of a Clarktype dissolved-oxygen electrode and an FMO-3 immobilized membrane. In order to amplify the biosensor output was applied. The FMO-3 biosniffer was used to measure TEA vapor.
(c) acetaldehyde is one of the specific malodor substances, and its maximum permitted concentration is defined as 25 ppm by ACGIH. The bio-sniffer device was constructed by coating a carbon and Ag/AgCl electrodes on a filter paper, and immobilizing enzyme (ALDH) to the carbon electrode. The ALDH sniffer was used in the gas phase to measure acetaldehyde vapor with the calibration range from 0.2 to 4.0 ppm with good gas selectivity which was attributed to the substrate specificity of ALDH. Less
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[Journal Article] BIOELECTRONIC SNIFFERS FOR FORMALDEHYDE IN THE GAS PHASE2005
Author(s)
Kohji MITSUBAYASHI, Genki NISHIO, Yoshihiro NAKAYAMA, Hirokazu AMAGAI, Hidenori WATANABE, Nicole JAFFREZIC-RENAULT, Thierry NOGUER and Jean-Louis MARTY
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Journal Title
International Journal of Environmental Analytical Chemistry, Intern. J. Environ. Anal. Chem 85
Pages: 917-925
Description
「研究成果報告書概要(欧文)」より
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