2007 Fiscal Year Final Research Report Summary
Roles of a Wnt activator, Ccd1, in neural development
| Project/Area Number |
17300098
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | University of Tsukuba |
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Principal Investigator |
MASU Masayuki University of Tsukuba, Graduate School of Comprehensive Hueman Sciences, Professor (20243032)
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| Co-Investigator(Kenkyū-buntansha) |
MASU-KEINO Kazuko University of Tsukuba, Graduate School of Comprehensive Hueman Sciences, Lecturer (50344883)
SHIOMI Kensuke University of Tsukuba, Graduate School of Comprehensive Hueman Sciences, Lecturer (00311598)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Wnt signaling / Beta-cateniin / DIX domain / CH domain / actin |
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| Research Abstract |
Wnt signaling plays important roles in axis formation, differentiation, growth, axon guidance, and synaptogenesis. Wnt signal is transduced via three pathways, beta-catenin pathway, PCP pathway, and Calcium pathway. Although many molecules involved in the Wnt pathways have been identified, many questions regarding the regulation of Wnt signaling remain unresolved. Recently we identified a novel protein Ccd1 (Coiled-coil-DIX1) as third DIX-domain-containing protein from zebrafish. We showed that Ccd1 activates Wnt/beta-catenin pathway both in vitro and in vivo, and that it regulates neural patterning in zebrafish embryos. We also found that many Ccd1 isoforms are generated via differential promoter usage and alternative splicing in mouse. Among them, a novel isoform, named Ccd1A, contains one calponin homology (CH) domain in the N-terminus in addition to the coiled-coil and DIX domains found in the original isoform (named Ccd1B). From zebrafish, we isolated second gene, Ccd2, which encode both A and B subtypes. When heterogouly expressed in cultured cell lines, Ccd1B protein is present as small vesicles throughout cells, Ccd2A protein is co-localized with actin fibers. In addition, the cytoplasmic localization of Dishevelled (Dvl) protein is altered by forced expression of Ccd1A and Ccd1B, suggesting that Ccd1 protein interacts with Dvl in the cell. Although we generated knockout mice, they did not show any obvious phenotypes. When we overexpressed Ccd2 in zebrafish embryos, they showed curly tail-down phenotype, suggesting that Ccd1 plays a role in midline signaling.
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Research Products
(81 results)
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[Journal Article] Mouse liaison for integrative brain research2007
Author(s)
Aiba, A., Inokuchi, K., Ishida, Y., Itohara, S., Kobayashi, K., Masu, M., Mishina, M., Miyakawa, T., Mori, H., Nakao, K., Obata, Y., Sakimura, K., Shiroishi, T., Wada, K., Yagi, T.
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Journal Title
Neurosci. Res. 58
Pages: 103-104
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic marking of hematopoietic stem and endothelial cells : Identification of the Tmtsp genes encoding a novel cell-surface protein with the thrombospondin-1 domain2006
Author(s)
Takayanagi, S., Hiroyama, T., Yamazaki, S., Nakajima, T., Morita, Y., Usui, J., Eto, K., Motohashi, T., Shiomi, K., Keino-Masu, K., Masu, M., Oike, Y., Mori, S., Yoshida, N., Iwama, A., and Nakauchi, H.
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Journal Title
Blood 107
Pages: 4317-4325
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling2006
Author(s)
Kamimura, K., Koyama, T., Habuchi, H., Ueda, R., Masu, M., Kimata, K., and Nakato, H.
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Journal Title
J. Cell Biol. 174
Pages: 773-778
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and differential expression of multiple isoforms of mouse Ccd1(Coiled-coil-DIX1), a positive regulator of Wnt signaling2005
Author(s)
Shiomi, K., Kanemoto, M., Keino-Masu, K., Yoshida, S., Soma, K., and Masu, M.
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Journal Title
Brain Res. Mol. Brain Res. 135
Pages: 169-180
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Heparan sulfate profile in the mice deficient for heparan sulfate 6-O-endosulfatases2007
Author(s)
Keino-Masu, K., Nagamine, S., Tanba, M., Ishimine, H., Ohto, T., KuniTa, S., Takahashi, S., Masu, M.
Organizer
The 30th Annual Meeting of the Molecular Biology Society of Japan and The 80th Annual Meeting of the Japanese Biochemical Society
Place of Presentation
Yokohama
Year and Date
20071211-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Association study of the sulfatase 1(SULF1) gene and bipolar disorder2007
Author(s)
Horiuchi, Y., Ishiguro, H., Koga, M., Inada, T., Muratake, T., Someya, T, Keino-Masu, K., Masu, M., Arinami, T.
Organizer
The 29th Annual Meeting for the Japanese Society of Biological Psychiatry and The 37th Annual Meeting for the Japanese Society of Neuropsychopharmacology
Place of Presentation
Sapporo
Year and Date
20070711-13
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Regulation of morphogenesis by heparan sulfate modifying enzymes, sulfFPs2007
Author(s)
Shiomi, K., Nagamine, S., Okada, T., Keino-Masu, K., Masu, M.
Organizer
The 40th Annual Meeting for the Japanese Society of Developmental Biologists and The 59th Annual Meeting of the Japan Society for Cell Biology, Mini-Symposium "Regulation of cell fate and tissue development by the extracellular matrix"
Place of Presentation
Fukuoka
Year and Date
20070528-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Autotaxin regulates endocytosis and vesicular trafficking in the mouse visceral endoderm cells2007
Author(s)
Koike. S., Keino-Masu, K., Ohto, T., Sugiyama, F., Takahashi, S., Masu, M.
Organizer
The 40th Annual Meeting for the Japanese Society of Developmental Biologists and The 59th Annual Meeting of the Japan Sociery for Cell Biology
Place of Presentation
Fukuoka
Year and Date
20070528-30
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Functional characterization of an exoenzyme autotaxin in the mouse early development2006
Author(s)
Koike, S., Keino-Masu, K., Ohto, T., Sugiyama, F., Takahashi, S., Masu, M.
Organizer
The 8th AEARU Joint Workshop on Life Science
Place of Presentation
Tsukuba
Year and Date
20061104-06
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Motor learning in the mice lacking a heparan sulfate 6-O-endosulfatase SufFP12006
Author(s)
Keino-Masu, K., Okamoto, T., Shutou, F, Yamazaki, N, Miyagawa, T, Ohto, T., Kunita, S., Takahashi, S., Nagao, S., Masu, M.
Organizer
The 29th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Kyoto
Year and Date
20060719-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Heparan sulfate 6-O-endosulfatase are required for normal brain development2006
Author(s)
Okada, T., Nagamine, S., Keino-Masu, K., Ohto, T., Kunita, S., Takahashi, S., Masu, M.
Organizer
The 29th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Kyoto
Year and Date
20060719-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cerebellar development in the mice lacking a heparan sulfate 6-O-endosulfatase2006
Author(s)
Ishibashi, N., Keino-Masu, K., Ohto, T., Kunita, S., Takahashi, S., Masu, M.
Organizer
The 29th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Kyoto
Year and Date
20060719-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cloning and characterization of the zebrafish Sufatase FP3gene2006
Author(s)
Suzuki, Y, Shiomi, K., Tamura, K., Uchida, H., Keino-Masu, K., Masu, M.
Organizer
The 29th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Kyoto
Year and Date
20060719-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Autotaxin is essential for blood vessel formation2006
Author(s)
Koike, S. Keino-Masu, K., Ohto, T., Sugiyama, F., Takahashi, S., and Masu, M.
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
Place of Presentation
Kyoto
Year and Date
20060618-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Motor learning in the mice lacking a heparan sulfate 6-O-endosulfatase SufFP12006
Author(s)
Keino-Masu, K., Okamoto, T, Shutou, F, Yamazaki, N, Miyagawa, T, Ohto, T., Kunita, S., Takahashi, S., Nagao, S., Masu, M.
Organizer
The 29th Japan Neuroscience Meeting Satellite Symposium "Mouse genetical manipulations as tools for understanding brain function"
Place of Presentation
Kyoto
Year and Date
2006-07-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Isthmin1 regulates Nodal signaling in zebrafish blastula and gastrula2005
Author(s)
Araki, I., Rabile, F., Lohrs, C., Pera, E., De Robertis, E., Masu, M., Brand, M.
Organizer
The l5th International Society of Developmental Biologist Congress
Place of Presentation
Sydney
Year and Date
20050903-07
Description
「研究成果報告書概要(欧文)」より
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