2007 Fiscal Year Final Research Report Summary
Basic studies on remodeling of fibrous tissues and their application to reconstruction of ligaments
| Project/Area Number |
17300145
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TAKAKUDA Kazuo Tokyo Medical and Dental University, Institute of Biomateiials and Bioengineering, Professor (70108223)
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| Co-Investigator(Kenkyū-buntansha) |
KOYAMA Yoshihisa Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Assistant Professor (70361714)
NAGAI Masahiro Tokyo Medical and Dental University, Institute of Biomaterials and Bioengineering, Assistant Professor (10013971)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Biomechanics / Biomaterials / Mechanical Stimuli / Tissue Engineering / Ligaments / Remodeling |
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| Research Abstract |
1. Design of devices for ligament reconstruction considering the tissue remodeling Polyester fabrics composed of sparsely aligned fibers were utilized as the model grafts for the artificial ligaments. the biological fixation was expected to be realized through the spontaneous bone ingrowth into the space between fibers. experimental anterior cruciate ligament reconstruction was performed with using the rabbit model. the mechanical test and histometric measurement demonstrated that the attachment strength and amount of bone area were significantly large in the group of chitin-coated grafts. thus biological fixation was successfully realized and the possibility of ACL reconstruction was demonstrated.
2. Biological fixation of percutaneous devices and dental implants with use of the guided remodeling of collagen fibers. Model devices were developed to which spontaneous anchoring of surrounding soft fibrous tissue was realized. implantation test with using rat's hind skin demonstrated the a
… More
ll specimens without anchoring structure dropped out of skin, whereas the specimens equipped with the anchoring structure did not Further, histological observation revealed the spontaneous anchoring of collagen fibers. such spontaneous anchoring is expected to be beneficial for percutaneous devices and dental implants.
3. In vitro remodeling of soft fibrous tissues under mechanical stimuli Femoral fascia of rat, a thin fibrous soft tissue surrounding muscle, in which orientation of collagen fibers can be monitored with a light microscope was cultured under the mechanical stimuli of static stretching. In case of the culture free from load, the orientation of fibers became random, and the toe region in the load-displacement curve disappeared. Contrarily, the specimens subjected to incremental small stretching demonstrated highly aligned collagen fibers and extended toe region. On the other hand, the tissue subjected to a large stretch demonstrated aligned collagen fibers and small toe region. Thus suitable magnitude of mechanical stimuli induced the realignment of collagen fibers and adoptive change of mechanical properties, whereas large stimuli do not trigger remodeling. Less
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