• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

Application of a new cell-adhesive and cell-migatory molecule, laminin-5B, to regenerative medicine

Research Project

Project/Area Number 17300157
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionYokohama City University

Principal Investigator

MIYAZAKI Kaoru  Yokohama City University, Kihara Institute for Biological Research, Professor, 木原生物学研究所, 教授 (70112068)

Co-Investigator(Kenkyū-buntansha) HIHASHI Shouichi  Yokohama City University, Kihara Institute for Biological Research, Assistant, 木原生物学研究所, 助手 (10275076)
NAGASHIMA Yoji  Yokohama City University, Medical School, Associate Professor, 医学部, 準教授 (10217995)
Project Period (FY) 2005 – 2006
Keywordslaminin / stem cell / mesenchymal stem cell / proliferation / differentiation / vascular endothelial cell / basement membrane / cell adhesion
Research Abstract

Laminins are a family of ECM proteins that are localized mainly in basement membranes and regulate various cellular functions such as adhesion, motility, proliferation, apoptosis and differentiation. More than 15 laminin isoforms consisting of different combinations of the three chains have been identified thus far. Among them, laminin-5 (α3A/B, β3, γ2 ; laminin-332), strongly promotes cellular adhesion and migration. Recently we established efficient expression systems of human recombinant laminin-5A (αA, β3, γ2) and laminin-5B (α3B, β3, γ2). In this study, we investigated the possibility to use these laminins for regenerative medicine. We also attempted to establish a system to produce a novel laminin isoform, laminin-6B (α3B, β1, γ1). Main results are as follows. 1) Mesenchymal stem cell (MSC) is thought to be an important cell type in the regenerative medicine, because it can differentiate into osteoblasts, chondrocytes, neural cells, muscle cells and some others. We found that laminin-5A and laminin-5B efficiently stimulated the growth of human MSCs in culture, while suppressing their chondrogenic differentiation. This suggest the possible use of laminin-5A/B as a growth-stimulatory supplement for the efficient in vitro growth of human MSCs. 2) The presence of laminin-6B is predicted, but it has never been identified. We found that this laminin existed in the basement membranes of micro-blood vessels in various human tissues. We also established an efficient expression system of this novel laminin by introducing cDNAs for human laminin α3B, β1, γ1 chains into HEK293 cells. (α3A, β3, γ2). This laminin may play an important role in angiogenesis and maintenance of vascular structures and functions.

  • Research Products

    (43 results)

All 2007 2006 2005 Other

All Journal Article (38 results) Book (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] The beta3 chain short arm of laminin-332 (laminin-5) induces matrix assembly and cell adhesion activity of laminin-511 (laminin-10).2007

    • Author(s)
      Nakashima, Y., Miyazaki, K.et al.
    • Journal Title

      J. Cell. Biochem. 100

      Pages: 545-556

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha 3beta 1 integrin-mediated cell migration.2007

    • Author(s)
      Zhao, Y., Miyazaki, K.et al.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 32122-32130

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Deletion of core fucosylation on alpha3beta1 integrin down-regualtes its function.2007

    • Author(s)
      Zhao, Y., Miyazaki, K.et al.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 38343-38350

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The short arm of laminin gamma2 chain of laminin-5 (laminin-332) binds syndecan-1 and regulates cellular adhesion and migration by suppressing 4 chain.bphosphorylation of integrin2007

    • Author(s)
      Ogawa, T., Miyazaki, K.et al.
    • Journal Title

      Mol. Biol. Cell 18

      Pages: 1621-1633

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Strong suppression of tumor growth by insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1)/TAF/mac25.2007

    • Author(s)
      Sato, Y., Miyazaki, K.et al.
    • Journal Title

      Cancer Sci. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Laminins in keratoconus, bullous keratopathy, normal and scarred human corneas.2007

    • Author(s)
      S Bystrom B., Miyazaki, K.et al.
    • Journal Title

      Histochem Cell Biol. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The b3 chain short arm of laminin-332 (laminin-5) induces matrix assembly and cell adhesion activity of laminin-511 (laminin-10).2007

    • Author(s)
      Nakashima, Y., Kariya, Y., Miyazaki, K.
    • Journal Title

      J.Cell.Biochem. 100

      Pages: 545-556

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The short arm of laminin g2 chain of laminin-5 (laminin-332) binds syndecan-1 and regulates cellular adhesion and migration by suppressing phosphorylation of integrin b4 chain.2007

    • Author(s)
      Ogawa, T., Tsubota, Y., Hashimoto, J., Miyazaki, K.
    • Journal Title

      Mol.Biol.Cell 18

      Pages: 1621-1633

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Plasma membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin-and fibronectin-derived signaling.2006

    • Author(s)
      Kato, K., Miyazaki, K.et al.
    • Journal Title

      Biochem. J. 394

      Pages: 647-656

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Unique biological activity and role in tumor growth and invasion.2006

    • Author(s)
      Miyazaki, K.
    • Journal Title

      Cancer Sci. 394

      Pages: 91-98

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reduced expression of laminin-5 in corneal epithelial cells under high glucose condition.2006

    • Author(s)
      Lu.W., Miyazaki, K
    • Journal Title

      Cornea 25

      Pages: 61-67

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Insulin-like growth factor-1 induces migration and expression of laminin-5 in cultured human corneal epithelial cells.2006

    • Author(s)
      Lee, H.K., Miyazaki, K.
    • Journal Title

      Invest. Ophthalmol Vis. Sci. 47

      Pages: 873-882

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332).2006

    • Author(s)
      Hashimoto, J., Miyazaki, K.et al.
    • Journal Title

      Stem Cells 24

      Pages: 2346-2354

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Plasma membrane-associated sialidase (NEU3) differentially regulates integrin-mediated cell proliferation through laminin-and fibronectin-derived signaling.2006

    • Author(s)
      Kato, K., Shiga, K., Yamaguchi, K., Hata, K., Kobayashi, T., Miyazaki, K., Saijo, S., Miyagi, T.
    • Journal Title

      Biochem.J. 394

      Pages: 647-656

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Laminin-5 : Unique biological activity and role in tumor growth and invasion.2006

    • Author(s)
      Miyazaki, K.
    • Journal Title

      Cancer Sci. 97

      Pages: 91-98

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reduced expression of laminin-5 in corneal epithelial cells under high glucose condition.2006

    • Author(s)
      Lu W, Ebihara N, Miyazaki K, Murakami A.
    • Journal Title

      Cornea. 25

      Pages: 61-67

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Insulin-like growth factor-1 induces migration and expression of laminin-5 in cultured human corneal epithelial cells.2006

    • Author(s)
      Lee, H.K., Lee, J.H., Kim, M., Kariya, Y., Miyazaki, K., Kim, E.K.
    • Journal Title

      Invest Ophthalmol Vis Sci. 47

      Pages: 873-882

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of proliferation and chondrogenic differentiation of human mesenchymal stem cells by laminin-5 (laminin-332).2006

    • Author(s)
      Hashimoto, J., Kariya, Y., Miyazaki, K.
    • Journal Title

      Stem Cells 24

      Pages: 2346-2354

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] N-acetylglucosaminyltransferase III antagonizes the effect of N-acetylglucosaminyltransferase V on alpha 3beta 1 integrin-mediated cell migration.2006

    • Author(s)
      Zhao, Y., Nakagawa, T., Itoh, S., Inamori, K., Isaji T, Kariya, Y., Kondo, A., Miyoshi, E., Miyazaki, K., Kawasaki, N., Taniguchi, N., Gu, J.
    • Journal Title

      J Biol Chem. 281(43)

      Pages: 32122-32130

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Deletion of core fucosylation on alpha3betal integrin down-regualtes its function.2006

    • Author(s)
      Zhao, Y., Itoh, S., Wang, X., Isaji, T., Miyoshi, E., Kariya Y, Miyazaki, K., Kawasaki, N., Taniguchi, N., Gu J.
    • Journal Title

      J Biol Chem. 281

      Pages: 38343-38350

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of biological activity and matrix assembly of laminin-5 by COOH-terminal, LG4-5 domain of alfa3 chain.2005

    • Author(s)
      Tsubota, Y., Miyazaki, K.et al.
    • Journal Title

      J. Biol. Chem. 280

      Pages: 14370-14377

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ly6 family member C4.4A binds laminins 1 and 5, associates with galectin-3 and supports cell migration.2005

    • Author(s)
      Paret, C., Miyazaki, K.et al.
    • Journal Title

      Int. J. Cancer 115

      Pages: 724-733

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nephrotic syndrome and aberrant expression of laminin isoforms in glomerular basement membranes in an infant with Herlitz junctional epidermolysis bullosa2005

    • Author(s)
      Hata, D., Miyazaki, K.et al.
    • Journal Title

      Pediatrics 116

      Pages: 601-607

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5.2005

    • Author(s)
      Hashimoto, J., Miyazaki, K.et al.
    • Journal Title

      Exp. Cell. Res. 310

      Pages: 256-269

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of cell adhesion and type VII collagen binding by beta3 chain short arm of laminin-5 : effect of its proteolytic cleavage.2005

    • Author(s)
      Nakashima, Y., Miyazaki, K.et al.
    • Journal Title

      J. Biochem. 138

      Pages: 539-552

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics.2005

    • Author(s)
      Amin, K., Miyazaki, K.et al.
    • Journal Title

      Respiratory Research 6

      Pages: 1-10

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A simplified laminin nomenclature.2005

    • Author(s)
      Aumailley, M., Miyazaki, K.et al.
    • Journal Title

      Matrix Biol. 24

      Pages: 326-332

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential expression of laminin isoforms in ovarian epithelial carcinomas suggesting different origin and providing tools for differential diagnosis.2005

    • Author(s)
      Maatta, M., Miyazaki, K.et al.
    • Journal Title

      J Histochem. Cytochem. 53

      Pages: 1293-1300

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Regulation of biological activity and matrix assembly of laminin-5 by COOH-terminal, LG4-5 domain of alfa3 chain.2005

    • Author(s)
      Tsubota, Y., Yasuda, C., Kariya, Y., Ogawa, T., Hirosaki, T., Mizushima, H., Miyazaki, K.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 14370-14377

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ly6 family member C4. 4A binds laminins 1 and 5, associates with galectin-3 and supports cell migration.2005

    • Author(s)
      Paret, C., Bourouba, M., Beer, A., Miyazaki, K., Schnolzer, M., Fiedler, S., Zoller, M.
    • Journal Title

      Int.J.Cancer 115

      Pages: 724-733

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Nephrotic syndrome and aberrant expression of laminin isoforms in glomerular basement membranes in an infant with Herlitz junctional epidermolysis bullosa2005

    • Author(s)
      Hata, D., Miyazaki, M., Seto, S., Kadota, E., Muso, E., Thkasu, K., Nakano, A., Tamai, K., Uitto, J., Nagata, M., Moriyama, K., Miyazaki, K.
    • Journal Title

      Pediatrics 116

      Pages: 601-607

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Laminin-5 suppresses chondrogenic differentiation of murine teratocarcinoma cell line ATDC5.2005

    • Author(s)
      Hashimoto, J., Ogawa, T., Tsubota, Y., Miyazaki, K.
    • Journal Title

      Exp.Cell.Res. 310

      Pages: 256-269

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of cell adhesion and type VII collagen binding by beta3 chain short arm of laminin-5 : effect of its proteolytic cleavage.2005

    • Author(s)
      Nakashima, Y., Kariya, Y., Yasuda, C., Miyazaki, K
    • Journal Title

      J.Biochem. 138

      Pages: 539-552

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Uncoordinated production of Laminin-5 chains in airways epithelium of allergic asthmatics.2005

    • Author(s)
      Amin, K., Janson, C., Seveus, L., Miyazaki, K., Virtanen, I., Venge, P.
    • Journal Title

      Respir.Res. 6

      Pages: 110

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] D. A simplified laminin nomenclature.2005

    • Author(s)
      Aumailley, M., Bruckner-Tuderman, L., Carter, W.G., Deutzmann, R., Edgar, D., Ekblom, P., Engel, J., Engvall, E., Hohenester, E., Jones, J.C., Kleinman, H.K., Marinkovich, M.P., Martin, G.R., Mayer, U., Meneguzzi, G., Miner, J.H., Miyazaki, K., Patarroyo, M., Paulsson, M., Quaranta, V., Sanes, J.R., Sasaki, T., Sekiguchi, K., Sorokin, L.M., Talts, J.F., Tryggvason, K., Uitto, J., Virtanen, I., von der Mark, K., Wewer, U.M., Yamada, Y., Yurchenco, P.
    • Journal Title

      Matrix Biol. 24

      Pages: 326-32

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differential expression of laminin isoforms in ovarian epithelial carcinomas suggesting different origin and providing tools for differential diagnosis.2005

    • Author(s)
      Maatta, M., Butzow, R., Luostarinen, J., Petajaniemi, N., Pihlajaniemi, T., Salo, S., Miyazaki, K., Autio-Harmainen, H., Virtanen I.
    • Journal Title

      J.Histochem.Cytochem. 53

      Pages: 1293-300

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Strong suppression of tumor growth by insulin-like growth factor binding protein-related protein 1 (IGFBP-rPl)/TAF/mac25.

    • Author(s)
      Sato, Y., Chen, Z., Miyazaki, K.
    • Journal Title

      Cancer Sci. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Laminins in keratoconus, bullous keratopathy, normal and scarred human corneas.

    • Author(s)
      Bystrom B., Virtanen, I., Rousselle, P., Miyazaki, K., Linden, C., Fatima Pedrosa Domelltif, F.P.
    • Journal Title

      Histochem Cell Biol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 上皮-間葉変換と癌の悪性増殖-細胞外マトリックス分子の役割。「実験医学」Vol.21,No.152006

    • Author(s)
      宮崎香
    • Total Pages
      6(2064-2070)
    • Publisher
      実験医学4月号 羊土社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 細胞外マトリックス分子と細胞接着。「タンパク質科学-構造・物性・機能」2005

    • Author(s)
      小川崇, 宮崎香(分担)
    • Total Pages
      12(555-566)
    • Publisher
      化学同人
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 細胞外マトリックス分子と癌の悪性増殖。「肝転移のすべて」(門田守人、松浦成昭/編)2005

    • Author(s)
      宮崎香(分担)
    • Total Pages
      6(44-49)
    • Publisher
      永井書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] ラミニン6B(ラミニン3B11)蛋白質2007

    • Inventor(s)
      宮崎香
    • Industrial Property Rights Holder
      横浜市立大学
    • Industrial Property Number
      2007-028897
    • Filing Date
      2007-02-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Patent(Industrial Property Rights)] ラミニン5を利用した間葉系幹細胞の培養技術2005

    • Inventor(s)
      宮崎香, 橋本絢子, 苅谷慶喜
    • Industrial Property Rights Holder
      横浜市立大学
    • Industrial Property Number
      2005-240814(PCT/JP2006/316545)
    • Filing Date
      20050823, (20060816)
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2008-05-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi