2007 Fiscal Year Final Research Report Summary
Protective effects of an inducer of molecular chaperon on disuse skeletal muscle
| Project/Area Number |
17300187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Himeji Dokkyo University (2006-2007)
Suzuka University of Medical Science (2005) |
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Principal Investigator |
FUJINO Hidemi Himeji Dokkyo University, Department of Physical Therapy, Professor (20278998)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIHARA Akihiko Kyoto University, Graduate School of Human and Environmental Studies, Professor (90184548)
TAKEDA Isao Himeji Dokkyo University, Department of Physical Therapy, Professor (00163402)
KAJIYA Fumihiko Kawasaki University of Medical Welfare, Department of Medical Engineering, Professor (70029114)
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| Project Period (FY) |
2005 – 2007
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| Keywords | rehabilitation / proteome / molecular signaling / teprenon / molecular chaperones / heat shock protei / skeletal muscle / disuse atrophy |
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| Research Abstract |
Geranylgeranylacetone (GGA) has been used in clinics for many years as a therapeutic agent for gastric ulcer and gastritis. GGA induces 70-kDa heat shock protein (HSP70) selectively, and HSPs are induced as an adaptative response in all cells and organisms upon exposure to a number of stresses. HSPs function as molecular chaperones involved in the correct folding and refolding of proteins, and thereby exert essential housekeeping and protective functions. In this study, we thus investigated the effects of GGA on skeletal muscle under normal and stressful conditions such as disuse atrophy by hindlimb unloading in rats. Skeletal muscles were isolated 24 hours after administration of GGA at 0,200,400 and 600 mg/kg. In soleus, gastrocnemius, plantaris and extensor digitorum longus muscles, increasing the dose up to 600 mg/kg did not result in a further increase in HSP72 expression. GGA primed other types of cells for enhanced induction of HSP70, when exposed to stress. In addition, we investigated the induction of HSP70 after administration of GGA in hindlimb unloading condition. The HSP72 levels decreased in the atrophied muscle compare with control, whereas these values in the GGA administrated muscles attenuated the decrease of HSP70 in hindlimb unloading condition. Although hindlimb unloading resulted in muscle atrophy, the loss of myofibrillar protein content was significantly less in the GGA administrated skeletal muscle. These results suggest that GGA may exhibit protective actions independently of Hsp70 induction. Additionally, these observations may also indicate that the administration of GGA could be one of the useful tools to attenuate muscle atrophy.
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Author(s)
Sugawara Y, Ando R, Kamioka H, Ishihara Y, Murshid SA, Hashimoto K, Kataoka N, Tsujioka K, Kajiya F, Yamashiro T, Takano-Yamamoto T.
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[Journal Article] Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator-activated receptor-gamma coactivator-lalpha of fibres in the soleus muscle of Zucker diabetic fatty rats.2007
Author(s)
Adachi T, Kikuchi N, Yasuda K, Anahara R, Gu N, Matsunaga T, Yamamura T, Mori C, Tsujimoto G, Tsuda K, Ishihara A.
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Journal Title
Exp Physiol 92
Pages: 449-455
Description
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[Journal Article] Changes of asymmetric dimethylarginine, nitric oxide, tetrahydrobiopterin, and oxidative stress in patients with acute myocardial infarction by medical treatments.2007
Author(s)
Yada T, Kaji S, Akasaka T, Mochizuki S, Ogasawara Y, Tanemoto K, Yoshida K, Kajiya F.
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Journal Title
Clin Hemorheol Microcirc 37
Pages: 269-276
Description
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[Journal Article] Impaired NO-mediated vasodilation with increased superoxide but robust EDHF function in right ventricular arterial microvessels of pulmonary hypertensive rats.2007
Author(s)
Kajiya M, Hirota M, Inai Y, Kiyooka T, Morimoto T, Iwasaki T, Endo K, Mohri S, Shimizu J, Yada T, Ogasawara Y, Naruse K, Ohe T, Kajiya P.
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Journal Title
Am J Physiol Heart Circ Physiol 292
Pages: H2737-2744
Description
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[Journal Article] Cardioprotective role of endogenous hydrogen peroxide during ischemia-reperfusion injury in canine coronary microcirculation in vivo. Am J Physiol Heart Circ Physiol 291: HI 138-1146,2006.2006
Author(s)
Yada T, Shimokawa H, Hiramatsu O, Haruna Y, Morita Y, Kashihara N, Shinozaki Y, Mori H, Goto M, Ogasawara Y, Kajiya F.
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Am J Physiol Heart Circ Physiol 291
Pages: H1138-1146
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[Journal Article] Celsior preserved cardiac mechanoenergetics better than popular solutions in canine hearts.2006
Author(s)
Oshima Y, Mohri S, Shimizu J, Iribe G, Imaoka T, Fujinaka W, Kiyooka T, Ishino K, Sano S, Kajiya F, Suga H.
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Journal Title
Ann Thorac Suig 81
Pages: 658-664
Description
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[Journal Article] Iris movement mediates vascular apoptosis during rat pupillary membrane regression.2006
Author(s)
Morizane Y, Mohri S, Kosaka J, Tone S, Kiyooka T, Miyasaka T, Shimizu J, Ogasawara Y, Shiraga F, Minatogawa Y, Sasaki J, Ohtsuki H, Kajiya F.
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Journal Title
Am J Physiol Regul Integr Comp Physiol 290
Pages: R819-825
Description
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[Journal Article] Beneficial effect of hydroxyfasudil, a specific Rho-kinase inhibitor, on ischemia/reperfusion injury in canine coronary microcirculation in vivo.2005
Author(s)
Yada T, Shimokawa H, Hiramatsu O, Kajita T, Shigeto F, Tanaka E, Shinozaki Y, Mori H, Kiyooka T, Katsura M, Ohkuma S, Goto M, Ogasawara Y, Kajiya F.
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Journal Title
J Am Coll Cardiol 45
Pages: 599-607
Description
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[Journal Article] Involvement of aldosterone and mineralocorticoid receptors in rat mesangial cell proliferation and deformability.2005
Author(s)
Nishiyama A, Yao L, Fan Y, Kyaw M, Kataoka N, Hashimoto K, Nagai Y, Nakamura E, Yoshizumi M, Shokoji T, Kimura S, Kiyomoto H, Tsujioka K, Kohno M, Tamaki T, Kajiya F, Abe Y.
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Journal Title
Hypertension 45
Pages: 710-716
Description
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[Journal Article] Evaluation of bioavailability of nitric oxide in coronary circulation by direct measurement of plasma nitric oxide concentration.2005
Author(s)
Neishi Y, Mochizuki S, Miyasaka T, Kawamoto T, Kume T, Sukmawan R, Tsukiji M, Ogasawara Y, Kajiya F, Akasaka T, Yoshida K, Goto M.
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Journal Title
Proc Nail Acad Sci U S A 102
Pages: 11456-11461
Description
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[Journal Article] Exogenous NO suppresses flow-induced endotheliumderived NO production because of depletion of tetrahydrobiopterin.2005
Author(s)
Mochizuki S, Sipkema P, Goto M, Hiramatsu O, Nakamoto H, Toyota E, Kajita T, Shigeto F, Yada T, Ogasawara Y, Kajiya F.
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Journal Title
Am J Physiol Heart Circ Physiol 288
Pages: H553-558
Description
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[Journal Article] Direct observation of epicardial coronary capillary hemodynamics during reactive hyperemia and during adenosine administration by intravital video microscopy.2005
Author(s)
Kiyooka T, Hiramatsu O, Shigeto F, Nakamoto H, Tachibana H, Yada T, Ogasawara Y, Kajiya M, Morimoto T, Morizane Y, Mohri S, Shimizu J, Ohe T, Kajiya F.
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Journal Title
Am J Physiol Heart Circ Physiol 288
Pages: H1437-1443
Description
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[Journal Article] Altered nano/micro-order elasticity of pulmonary artery smooth muscle cells of patients with idiopathic pulmonary arterial hypertension.
Author(s)
Nakamura K, Shimizu J, Kataoka N, Hashimoto K, Ikeda T, Fujio H, Ohta-Ogo K, Ogawa A, Miura A, Mohri S, Nagase S, Morita H, Kusano KF, Date H, Matsubara H, Mochizuki S, Kajiya F, Ohe T.
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Journal Title
Int J Caidiol 2008 [Epub ahead of print]
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Impaired Coronary Capillary Hemodynamics with Decreased Glycocalyx Thickness and Irregular Inner Wall in Right Ventricle of Pulmonary Hypertensive Rats.2005
Author(s)
Kajiya M, Morimoto T, Hirota M, Inai Y, Kiyooka, T, Hashiba k, Iwasaki T, Fujino H, Kohzuki H, Mohri S, Shimizu J, Ohtsuka A, Ohe T, Kajiya F.
Organizer
The American Heart Association Scientific Sessions is the premier international cardiovascular research and instructional gathering
Place of Presentation
USA
Year and Date
20050000
Description
「研究成果報告書概要(欧文)」より
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