2007 Fiscal Year Final Research Report Summary
Bioimaging profiles in molecular and cellular adaptations of calcium release units (CRUs) in exercise trained rat skeletal muscle cells
| Project/Area Number |
17300210
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | National Institute of Fitness and Sports in Kanoya |
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Principal Investigator |
TAKEKURA Hiroaki National Institute of Fitness and Sports in Kanoya, Sports Performance, Professor (00206963)
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| Co-Investigator(Kenkyū-buntansha) |
KASUGA Norikatsu Aichi University of Education, Physical Education, Professor (60152659)
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| Project Period (FY) |
2005 – 2007
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| Keywords | skeletal muscle / membrane system / excitation contraction coupling / transverse tubule / sarcoplasmic reticulum / triad / electron microscope / development / myotendinous junction |
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| Research Abstract |
The transverse (t) -tubule is responsible for the rapid inward spread of excitation from the sarcolemma to the inside of the muscle fiber, and the compartments of the t-tubule become highly and regularly organized during development. Although it is known that skeletal muscle fibers lengthen by adding sarcomeres at the myotendinous junction (MTJ) during development, no specific model exists for the assembly of new t-tubule architecture at the MTJ. We performed an electron-microscopic examination of the assembly of t-tubule architecture at the MTJ in developing rat skeletal muscle fibers. Although the longitudinally oriented t-tubule elements represent only a small fraction of the total t-tubule system in adult muscle fibers, they were observed at both A-band and I-band regions of middle and MTJ regions in early developmental stages, and gradually disappeared in the middle regions of muscle fibers during development ; however, they remained in the MTJ even in adult muscle fibers. The frequency of pentads and heptads (two or three t-tubule elements with three or four elements of terminal cisternae, closely aligned with terminal cisternae of the sarcoplasmic reticulum) decreased during development, with sudden decrease between 7 and 10 weeks of age in the middle regions. Interestingly, although the frequency of decrease appeared to be higher in the middle region than in the MTJ regions in early (3- to 7-week) development, this pattern reversed, and the frequency of decrease was higher in the MTJ in later development (after 10 weeks of age). The MTJ maintained the features of immature membrane systems involved in e-c coupling much longer than the middle region of the fiber during development. The assembly of t-tubule architecture during postnatal development thus follows different processes in the middle and MTJ regions of skeletal muscle fibers.
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Research Products
(39 results)
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[Journal Article] Assembly of transverse tubule architectures at middle and myotendinous junctional regions in developing rat skeletal muscle fiber2007
Author(s)
Yamashita, S., McGrath, K.F., Yuki, A., Tamaki, H., Kasuga, N.and Takekura, H.
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Journal Title
J.Muscle Res.Cell Motility 28
Pages: 141-151
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Assembly of transverse tubule architectures at middle and myotendinous junctional regions in developing rat skeletal muscle fiber2007
Author(s)
Yamashita, S., McGrath, K. F., Yuki, A., Tamaki, H., Kasuga, N., Takekura, H
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Journal Title
J. Muscle Res. Cell Motility, reviewed 28
Pages: 141-151
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Bidirect signaling between calcium channels of skeletal muscle requires multiple direct and indirect interactions2006
Author(s)
Sheridan, D.C., Takekura, H., Franzini-Armstrong, C., Beam, K.G., Allen, P.D., Perez, C.F.
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Journal Title
Proc.Natl.Acad.Sci.USA 103
Pages: 19760-19765
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] lasticity of neuromuscular junction architectures in rat slow and fast muscle fibers following temporary denervation and reinnervation processes2006
Author(s)
Nishizawa, T., Yamashita, S., McGrath, K.F., Tamaki, H., Kasuga, N., Takekura, H.
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Journal Title
J.Muscle Res.Cell Motility 27
Pages: 607-615
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Fiber type specificity, size, myonuclear number and domain in slow and fast muscles in adult mice2006
Author(s)
Suzuki, I., Komiya, H., Maeda, J., Kasuga, N., Takemiya, T.
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Journal Title
Adv.Exerc.Sports Physiol. 12
Pages: 35-42
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Plasticity of neuromuscular junction architectures in rat slow and fast muscle fibers following temporary denervation and reinnervation processes2006
Author(s)
Nishizawa, T., Yamashita, S., McGrath, K.F., Tamaki, H., Kasuga, N., Takekura, H
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Journal Title
J. Muscle Res. Cell Motility, reviewed 27
Pages: 607-615
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Bidirect signaling between calcium channels of skeletal muscle requires multiple direct and indirect interactions2006
Author(s)
Sheridan, D. C., Takekura, H., Franzini-Armstrong, C., Beam, K. G., Allen, P. D., Perez, C. F
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Journal Title
Proc. Natl. Acad. Sci. USA, reviewed 103
Pages: 19760-19765
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Habitual exercise induced resistance to oxidative stress2005
Author(s)
Nakatani, K., Komatsu, M., Kato, Y., Yamanaka, T., Takekura, H., Wagatsuma, A., Aoyama, K., Xu, B., Hirano, T., Kasai, H., Takeuchi, T.
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Journal Title
Free Radical Res. 39
Pages: 905-911
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Habitual exercise induced resistance to oxidative stress2005
Author(s)
Nakatani, K. Komatsu, M. Kato, Y. Yamanaka, T. Takekura, H. Wagatsuma, A. Aoyama, K. Xu, B. Hirano, T. Kasai, H. Ando, S. Takeuchi, T
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Journal Title
Free Radical Res., reviewed 39
Pages: 905-911
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Serotonin release of frontal cortex in human brain after high intensity exercise2007
Author(s)
Fujimoto, T., Kimura, Y., Kikuchi, J., Ishiwata, K., Ishii, K., Oda, K., Ogawa, S., Sensui, H., Nagamatsu, T., Ito, M., Tashiro, M., Nagatomi, R., Takekura, H
Organizer
12th Annual Congress ECSS
Place of Presentation
Jyvaskyla, Finland
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A novel form of retrograde signaling between RyR3 and the DHPR in skeletal muscle2007
Author(s)
Sheridan, D.C., Beam, K.G., Takekura, H., Franzini-Armstrong, C., Allen, P.D., Perez, C.F
Organizer
51st Annual Meering of the Biophysical Society
Place of Presentation
Baltimore, MD, USA
Year and Date
20070000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The relation between hippocampal structure and psychological well-being in exercising and non-exercising students2006
Author(s)
Fujimoto, T., Sensui, H., Takekura, H., Gondo, Y., Ono, Y., Kikuchi, J., Kinomura, S., Tashiro, M., Itoh, M., Nagamatsu, T., Nagatomi, R
Organizer
11th Annual Congress ot the ECSS
Place of Presentation
Lausanne, Switzerland(Book of Abstract)
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The influence of exercise on hippocampal structure and psychological well- veing in healthy young people2006
Author(s)
ensui, H., Fujimoto, T., Gondo, Y., Ono, Y., Ogawa, S., Kinomura, S., Tashiro, M., Itoh, M., Takekura, H., Nagatomi, R., Nagamatsu, T
Organizer
11th Annual Congress ot the ECSS
Place of Presentation
Lausanne, Switzerland(Book of Abstract)
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Whole-body energy consumtion map constructed by PET during some movement in daily life. Proc.2005
Author(s)
Fujimto, T., Ishii, K., Ito, M., Sensui, H., Ono, Y., dos Santos, T.R., Tashiro, M., Tanabe, K., Takekura, H., Nagotomi, R
Organizer
10th Annual Congress of the European College of Sports Science Belgrade
Place of Presentation
Serbia-Montenegro
Year and Date
20050000
Description
「研究成果報告書概要(欧文)」より
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