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2007 Fiscal Year Final Research Report Summary

Precise Analyses on the Enzymes Participationg in Fundamental Pathways in the Biosynthesisi of Isoprenoids

Research Project

Project/Area Number 17310122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionTohoku University

Principal Investigator

KOYAMA Tanetoshi  Tohoku University, Tohoku University, Institute of Multidisciplinary Research for Advanced Materials, Professor (20089808)

Co-Investigator(Kenkyū-buntansha) NOIKE Motoyoshi  Institute of Multidisciplinary Research for Advanced Materials, 多元物質科学研究所, Assistant Professor (20420010)
TAKAHASHI Seiji  Institute of Multidisciplinary Research for Advanced Materials, 多元物質科学研究所, Assistant Professor (90343061)
Project Period (FY) 2005 – 2007
KeywordsIsoprenoid Biosynthesis / Prenyltransferase / Natural Rubber Biosynthesis Farnesyl Diphosphate / Prenyl Chain Elongating Enzyme / Undecaaprenyl Diphosphate / Farnesyl Diphosphate / Dolichol
Research Abstract

[Studies on the Prenyl Chain Determination Mechanism of cis-Prenyltransferases] The ultimate chain length determination mechanism of cis-prenyl diphosphate syntheses was uncovered through structural manupulation of undecaprenyl diphosphate synthase derived from Micrococcus luteus B-P 26, based on comparisons between structures of various cis-prenyltransferases. Amino acid residues located in proximity of the substrate binding site as well as the charged residues inserted into the helix-III region are very important for the chain length determination mechanisms.
[Studies on the Substrate Binding Mechanisms of Prenyl Chain Elongating Enzymes] The methyl group at the 3-position of allylic substrates for prenyl chain elongating enzymes is found to be very important for the catalytic function as well as the substrate recognition mechanisms. According to the data on X-ray crystal structure determination analysis of undecaprenyl diphosphate synthase, three meta-stable structures, open-, medium-, and dosed forms of the helix-III area were found to be present, suggesting that this structural change makes the flexibility of helix -III to continue the chain elongation reaction repeatedly in the polyprenyl chain elongating prenyltransferase reaction.
[Studies on the Biosynthesis of Natural Rubber] Washed small rubber particles prepared by gel filtration of fresh Hevea brasiliensis, latex was found to have high rubber transferase activity when isopentenyl diphosphate and an allylic substrate were present in the reaction mixture. The enzymatic ally synthesized rubber molecule showed a typical bimodal molecular weight distribution pattern in gel permeation chromatography analysis, which was similar to those of the endogenous rubber with peaks at around 10^6 and 10^5.

  • Research Products

    (47 results)

All 2008 2007 2006 2005 Other

All Journal Article (21 results) (of which Peer Reviewed: 10 results) Presentation (23 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Selectivity in substrate-enzyme complexation studied by surface forces measurement2008

    • Author(s)
      Takehiro Suzuki
    • Journal Title

      Colloid Polym. Sci. 286

      Pages: 107-112

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Kinetic studies of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase reaction using 3-desmethyl allylic substrate analogs2008

    • Author(s)
      Keitaro Fujikura
    • Journal Title

      Biosci. Biotechnol. Biochem. 72

      Pages: 851-855

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Kinetic studies of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase reaction using 3-desmethy1 allylic substrate analogs2008

    • Author(s)
      K. Fujikura
    • Journal Title

      Biosci. Biotechnol. Biochem. 72

      Pages: 851-855

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Novel fluorescent analogues for transmembrane movement study of polyprenyl phosphates2007

    • Author(s)
      K. Koseki
    • Journal Title

      Bioorg. Med. Chem. Lett. 17

      Pages: 946-950

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Cloning and expression of the gene encoding solanesyl diphosphate synthase from Heveabrasiliensis2007

    • Author(s)
      A. Phatthiya
    • Journal Title

      Plant Science 172

      Pages: 824-831

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Biotransformation of prenyl alcohols by cultrued cells of Cucurbita maxima2007

    • Author(s)
      M. Nakagi
    • Journal Title

      J. Mol. Catal. B:Enzymatic 47

      Pages: 33-36

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Substrate Specificities of Wild and Mutated Farnesyl Diphosphate Synthases from Bacillus stearothermophilus with Artificial Substrates2007

    • Author(s)
      M. Nagaki
    • Journal Title

      Biosci. Biothechnol. Biochem. 71

      Pages: 1657-1662

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Explorhlg biosynthetic diversity with trichodiene synthase2007

    • Author(s)
      L.S. Vedula
    • Journal Title

      Arch. Biochem. Biophys. 466

      Pages: 260-266

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Novel fluorescent analogues for transmembrane movement study of Polyprenylphosphates2007

    • Author(s)
      K. Koseki
    • Journal Title

      Bioorg. Med. Chem. Lett. 17

      Pages: 946-950

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning and expression of the gene enoding solanesyl diphosphate synthase from Hevea brasiliensis2007

    • Author(s)
      Phatthiya, S.
    • Journal Title

      Plant Science 172

      Pages: 824-831

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Biotransformation of preny alcohols by cultured cells of Cucurbita maxima2007

    • Author(s)
      M. Nagaki
    • Journal Title

      J. Mol. Catal. B: Enzymatic 47

      Pages: 33-36

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Substrate Specificities of Wild and Mutated Farnesyl Diphosphate Synthases from Bacillus stearothermophilus with Artificial Substrates2007

    • Author(s)
      M. Nagaki
    • Journal Title

      Biosic. Biothechnol. Biochem. 71

      Pages: 1657-1662

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Exploring biosynthetic diversity with trichodiene synthase2007

    • Author(s)
      L. S. Vedula
    • Journal Title

      Arch. Biochem. Biophys 466

      Pages: 260-266

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Direct Observation of Substrate-Enzyme Complexation by Surface Forces Measurement2006

    • Author(s)
      T. Suzuki
    • Journal Title

      J. Am. Chem. Soc. 128

      Pages: 15209-15214

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Manipulation of prenyl chain length determination mechanism of cis-prenyltransferases2006

    • Author(s)
      Y. Khaxel
    • Journal Title

      FEBS Journal 273

      Pages: 647-657

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Direct Observation of Substrate-Enzvme Complexation bv Surface Forces Measurement2006

    • Author(s)
      T. Suzuki
    • Journal Title

      J. Am. Chem. Soc. 128

      Pages: 15209-15214

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Manipulation ofprenyl chain length determination mechanism of cis-prenyltransferases2006

    • Author(s)
      Y. Kharel
    • Journal Title

      FEBS Journal 273

      Pages: 647-657

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Takahashi, Seiji
    • Journal Title

      Fiber, Superbiomimetics, Constructions of Novel Biotechnology in Near Future(NTS)

      Pages: 147-153

  • [Journal Article] KaPPA-View. A Web-Based Analysis Tool for Integration of Transcript and Metabolite Data on Plant Metabolic Pathway Maps2005

    • Author(s)
      Toshiaki Tokimatsu
    • Journal Title

      Plant Physiology 138

      Pages: 1289-1300

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] KaPPA-View. AWeb-Based Analysis Tool for Integration ofTranscript and Metabolite Data on Plant Metabolic Pathway Maps2005

    • Author(s)
      T. Tokimatsu
    • Journal Title

      Plant Physiology 138

      Pages: 1289-1300

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 「研究成果報告書概要(欧文)」より2005

    • Author(s)
      Sagami, Hiroshi
    • Journal Title

      Chemical Lifesciences (in Japanese)(Asakura Shoten)

      Pages: 11-62

  • [Presentation] In vitro Synthesis of High Molecular Weight Rubber by Small Rubber Particles in Hevea brasiliensis Latex2007

    • Author(s)
      Porntip Rojruthai
    • Organizer
      BIOTRANS 2007
    • Place of Presentation
      Oviedo, Spain
    • Year and Date
      2007-07-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Product chain-length determination mechanism of cis-farnesyl diphosphate synthase2007

    • Author(s)
      Motoyoshi Noike
    • Organizer
      BIOTRANS 2007
    • Place of Presentation
      Oviedo, Spain
    • Year and Date
      2007-07-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Synthesis and Evaluation of Geranyl Diphosphate Analogs Having Oxygen Atom in their Prenyl Chain for Farnesyl Diphosphate Synthase and Its Mutated Enzymes2007

    • Author(s)
      Y. Maki
    • Organizer
      BIOTRANS 2007
    • Place of Presentation
      Oviedo, Spain
    • Year and Date
      2007-07-12
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Gene Cloning and Functional Analysis of Novel Short-Chain cis-Transferases2007

    • Author(s)
      Takanori Ambo
    • Organizer
      BIOTRANS 2007
    • Place of Presentation
      Oviedo, Spain
    • Year and Date
      2007-07-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Structure and function of cis-prenyl chain elongating enzymes.2007

    • Author(s)
      Tanetoshi Koyama
    • Organizer
      50th anniversary of work of Professor Tadeuz Chojnacki
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2007-06-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Structure and function of cis-prenyl chain elongating enzymes2007

    • Author(s)
      Tanetoshi Koyama
    • Organizer
      50th anniversary of work of Professor Tadeuz Chojnacki
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2007-06-29
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] cis-Prenyltransferases for Natural Rubber Biosynthesis in Hevea brasiliensis Latex2007

    • Author(s)
      Tanetoshi Koyama
    • Organizer
      TERPNET 2007
    • Place of Presentation
      Strasbourg, France
    • Year and Date
      2007-04-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional analysis of a cis-prenyltransferase AtCPT5 in the stress responses2007

    • Author(s)
      Seiji Takahashi
    • Organizer
      TERPNET 2007
    • Place of Presentation
      Strasbourg, France
    • Year and Date
      2007-04-30
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Studies on the flippase in the biosynthesis of bacterial celll wall2006

    • Author(s)
      Shunsuke Seki
    • Organizer
      11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Enzymatic synthesis of natural rubber by the small rubber particle of Hevea brasiliensis latex2006

    • Author(s)
      Porntip Rojruthai
    • Organizer
      11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan.
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional analysis of a novel medium-chain cis-prenyltransferase in abiotic stress response of Arabidonsis thaliana2006

    • Author(s)
      Seiji Takahashi
    • Organizer
      11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan.
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Mechanism of product chain-length determination in short-type cis-prenyltransferase2006

    • Author(s)
      Motoyoshi Noike
    • Organizer
      11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan.
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Enzymatic synthesis of natural rubber by the small rubber particle of Hevea brasiliensis latex2006

    • Author(s)
      Porntip Rojruthai
    • Organizer
      11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Functional analysis of a novel medium-chain cis-prenyltransferase in abiotic stress response of Arabidopsis thaliana2006

    • Author(s)
      Seiji Takahashi
    • Organizer
      11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Mechanism of product chain-length determination in short-type cis-prenyltransferase2006

    • Author(s)
      Motoyoshi Noike
    • Organizer
      11th FAOBMB Congress
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2006-06-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Molecular mechanisms of cis-prenyl chain elongating enzymes.2005

    • Author(s)
      Tanetoshi Koyama
    • Organizer
      International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, U.S.A.
    • Year and Date
      2005-12-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular mechanisms of cis-prenyl chain elongating enzymes2005

    • Author(s)
      Tanetoshi Koyama
    • Organizer
      International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Honolulu, U.S.A.
    • Year and Date
      2005-12-16
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Study on the synthesis of 5-methylfarnesol by use of farnesyl diphosphate synthase2005

    • Author(s)
      Masahiko Nagaki
    • Organizer
      BIOTRANS 2005
    • Place of Presentation
      Delft, The Netherlands
    • Year and Date
      2005-07-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Study on the synthesis of 5-methylfarnesol by use of farnesyl diphsphate synthase2005

    • Author(s)
      Masahiko Nagaki
    • Organizer
      BIOTRANS 2005
    • Place of Presentation
      Delft, The Netherlands
    • Year and Date
      2005-07-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Manipulation of Full-Stop Mechanism of cis-Prenyl Chain Elongating Enzymes2005

    • Author(s)
      Yugesh Kharel
    • Organizer
      BIOTRANS 2005
    • Place of Presentation
      Delft, The Netherlands
    • Year and Date
      2005-07-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Comparative Study on Substrate Specificities of Three Medium-Chain Prenyl Diphosphate Synthases2005

    • Author(s)
      Y. Maki
    • Organizer
      BIOTRANS 2005
    • Place of Presentation
      Delft, The Netherlands
    • Year and Date
      2005-07-05
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of physiological function of cis-prenyltransferases and their products in Arabidopsis thaliana2005

    • Author(s)
      Seiji Takahashi
    • Organizer
      TERPNET 2005
    • Place of Presentation
      Wageningen, The Netherlands
    • Year and Date
      2005-04-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Manipulation of Prenyl Chain Length Determination Mechanism of cis-Prenyltransferases2005

    • Author(s)
      Yugesh Kharel
    • Organizer
      TERPNET 2005
    • Place of Presentation
      Wageningen, The Netherlands
    • Year and Date
      2005-04-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] ファイバー:スーパーバイオミメテックス〜近未来の新技術創生〜2006

    • Author(s)
      高橋征司
    • Total Pages
      147-153
    • Publisher
      NTS
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 生命化学:第2章 遺伝子と酵素、第3章 生体物質の化学、第4章 代謝反応と生合成2005

    • Author(s)
      佐上 博
    • Total Pages
      11-62
    • Publisher
      朝倉書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.tagen.tohoku.ac.jp/labo/koyama/main-j.html

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Published: 2010-02-04  

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