2007 Fiscal Year Final Research Report Summary
Nano-organization of Photosynthetic Light-Harvesting Supramolecular Complexes and their Functional Analysis
| Project/Area Number |
17350055
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer chemistry
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
NANGO Mamoru Nagoya Institute of Technology, Material Science and Engineering, Professor (90109893)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Keiji Nagoya Institute of Technology, Material Science and Engineering, Associate Professor (90158155)
DEWA Takehisa Nagoya Institute of Technology, Material Science and Engineering, Research Associate (70335082)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Photosynthesis / Antenna-pigment complex / Self-assembly / lipid bilayer / Photoinduced electric current |
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| Research Abstract |
When light energy is absorbed in vivo by purple bacterial light-harvesting(LH) complexes it is rapidly transferred to the reaction centers(RC) where light energy is efficiently converted into useful chemical energy. It is important to reveal the functional details in the molecular level for understanding the fundamentals of energy conversion system in nature. Previous attempts to produce an artificial, energy-converting electrode system used either the LH1 or RC complex immobilized on the electrodes where the distance and orientation of their complexes were controlled. On the basis of the knowledge, in this study, we prepared various. photosynthetic antenna proteins and their model polypeptides via chemical synthesis and modern biosynthetic manufacturing methods to construct the light-harvesting protein complex onto electrodes. Energy and electron transfer of the constructed assemblies on gold electrodes modified with alkanethiols were evaluated by simultaneous measurements, electrochemical-spectroscopic and total internal reflection fluorescence(TIRF)-spectroscopic techniques. Together with results of structural information of the LH protein complexes from X-ray crystallography and AFM, we examind the functional relation with the dynamic structure of the LH complexes involved in the primary reactions of bacterial photosynthesis and how these complexes affect the efficiency of electron transfer between these complexes and the electrode.
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Research Products
(261 results)
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[Journal Article] Disappearance of the angiogenic potential of endothelial cells caused by Argonaute2 knockdown2008
Author(s)
T. Asai, Y. Suzuki, S. Matsushita, S. Yonezawa, J. Yokota, Y. Katanasaka, T. Ishida, T. Dewa, H. Kiwada, M. Nango, N. Oku
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Journal Title
Biochem. Biophys.Res. Commun (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization by Stark Spectroscopy of Reconstituted Light-harvesting 1 Complex of Purple Photosynthetic Bacterium Rhodospirillum rubrum with the Polypeptides, Bacteriochlorophyll a, and Carotenoids2008
Author(s)
K. Nakagawa, A. Mizuno, S. Suzuki, R. Fujii, T. Dewa, A. T. Gardiner, R. J. Cogdell, M. Nango, H. Hashimoto
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Journal Title
Carotenoid. Sci (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular Assembly of Artificial Photosynthetic Antenna Core Complex on an Amino-terminated ITO Electrode2007
Author(s)
Y. Suemori, K. Fujii, M. Ogawa, Y. Nakamura, K. Shinohara, K. Nakagawa, M. Nagata, K. Iida, T. Dewa, K. Yamashita, and M. Nango
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Journal Title
Colloid Surf. B 56
Pages: 182-187
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Molecular Assembly of Artificial Photosynthetic Antenna Core Complex on an Amino-terminated ITO Electrode2007
Author(s)
Y. Suemori, K. Fujii, M. Ogawa, Y. Nakamura, K. Shinohara, K. Nakagawa, M. Nagata, K. Iida, T. Dewa, K. Yamashita, M. Nango
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Journal Title
Colloid Surf. B 56
Pages: 182-18
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Self-Assenmbled Monolayer of Light-harvesting Core Complexes of Photosynthetic Bacteria on an Amino-Terminated ITO Eiectrode2006
Author(s)
Y. Suemori, M. Nagata, Y. Nakamura, K. Nakagawa, A. Okuda, J. Inagaki, K. Shinohara, M. Ogawa, K. Iida, T. Dewa, K. Yamashita, A. T. Gardiner, R. J. Cogdell, and M. Nango
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Journal Title
Photosynthesis Res. 90
Pages: 17-21
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Self-Assenmbled Monolayer of Light-harvesting Core Complexes of Photosynthetic Bacteria on an Amino-Terminated ITO Electrode2006
Author(s)
Y. Suemori, M. Nagata, Y. Nakamura, K. Nakagawa, A. Okuda, J. Inagaki, K. Shinohara, M. Ogawa, K. Iida, T. Dewa, K. Yamashita, A. T. Gardiner, R. J. Cogdell, M. Nango
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Journal Title
Photosynthesis Res 90
Pages: 17-21
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Design and Expression of Cysteine-Bearing Hydrophobic Polypeptides and Their Self-Assembling Properties with Bacteriochlorophyll a Derivatives as a Mimic of Bacterial Photosynthetic Antenna Complexes. Effect of Steric Confinement and Orientation of the Polypeptides on the Pigment/Polypeptide Assembly Process2005
Author(s)
T. Dewa, T. Yamada, M. Ogawa, M. Sugimoto, T. Mizuno, K. Yoshida, Y. Nakao, M. Kondo, K. Iida, K. Yamashita, T. Tanaka, and M. Nango
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Journal Title
Biochemistry 44
Pages: 5129-5139
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Efficient Peroxide Decoloration of C. I. Acid Orange 7 Catalyzed by Manganese Mesoporphyrin Dimer Derivatives in Micellar Solutions2005
Author(s)
M. Kondo, N. Kawashima, T. Mitsui, S. Ito, S. Ishigure, T. Hashimoto, R. Umemura, T. Dewa, K. Yamashita, H. Mino, S. Ito, and M. Nango
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Journal Title
Chem. Lett.
Pages: 1592-1593
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Efficient Peroxide Decoloration of C.I. Acid Orange 7 Catalyzed by Manganese Mesoporphyrin Dimer Derivatives in Micellar Solutions2005
Author(s)
M. Kondo, N. Kawashima, T. Mitsui, S. Ito, S. Ishigure, T. Hashimoto, R. Umemura, T. Dewa, K. Yamashita, H. Mino, S. Ito, M. Nango
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Journal Title
Chem. Lett
Pages: 1592-1593
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Design and Expression of Cysteine-Bearing Hydrophobic Polypeptides and Their Self-Assembling Properties with Bacteriochlorophyll a Derivatives as a Mimic of Bacterial Photosynthetic Antenna Complexes. Effect of Steric Confinement and Orientation of the Polypeptides on the Pigment/Polypeptide Assembly Process2005
Author(s)
T. Dewa, T. Yamada, M. Ogawa, M. Sugimoto, T. Mizuno, K. Yoshida, Y. Nakao, M. Kondo, K. Iida, K. Yamashita, T. Tanaka, M. Nango
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Journal Title
Biochemistry 44
Pages: 5129-5139
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization by Stark Spectroscopy of Reconstituted Light-harvesting 1 Complex of Purple Photosynthetic Bacterium Rhodospirillum rubrum with the Polypeptides, Bacteriochlorophyll α, and Carotenoids
Author(s)
K. Nakagawa, A. Mizuno, S. Suzuki, R. Fujii, T. Dewa, A. T. Gardiner, R. J. Cogdell, M. Nango, and H. Hashimoto
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Journal Title
Carotenoid. Sci. (in press)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Disappearance of the angiogenic potential of endothelial cells caused by Argonaute2 knockdown
Author(s)
T. Asai, Y. Suzuki, S. Matsushita, S. Yonezawa, J. Yokota, Y. Katanasaka, T. Ishida, T. Dewa, H. Kiwada, M. Nango, and N. Oku
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Journal Title
Biochem. Biophys. Res. Commun. (in press)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Presentation] Assembly of the light-harvesting/reaction center complexes of photosynthetic bacteria onto a gold substrate2007
Author(s)
T. Sakurai, Y. Suemori, M. Hatasa, O. Goto, K. Iida, T. Dewa, K. Yamashita, H. Tanaka, T. Ogawa, H. Hashimoto, and M. Nango
Organizer
7th International Conference on Tetrapyrrole Photoreceptors in photosynthetic Organisms
Place of Presentation
Kyoto, Japan
Year and Date
20071200
Description
「研究成果報告書概要(和文)」より
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[Presentation] Assembly of the light harvesting / reaction center complexes of photosynthetic bacteria onto a gold substrate2007
Author(s)
T. Sakurai, Y. Suemon, M. Hatasa, O. Goto, K. Iida, T. Dewa, K. Yamashita, H. Tanaka, T. Ogawa, H. Hashimoto, M. Nango
Organizer
7th International Conference on Tetrapyrrole Photoreceptors in photosynthetic Organisms
Place of Presentation
Kyoto, Japan
Year and Date
20071200
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Organization of a bacterial photosynthetic light harvesting complex in tetherd lipid bilayers2007
Author(s)
T. Takeuchi, R. Sugiura, A. Hiro, T. Dewa, Y. Suemon, T. Dewa, K. Yamashita, R. Tero, T. Urisu, M. Nango
Organizer
56th Symposium on Macromolecules
Place of Presentation
Nagoya, Japan
Year and Date
20070900
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Peroxidation Catatlyzed by Chlorophyll derivatives in Lipid Bilayer2007
Author(s)
Y. Kondo, T. Mitsui, S. Ito, T. Joke, Y. Takeuchi, S. Ishigure, M. Kondo, T. Dewa, K. Yamashita, M. Nango
Organizer
56th Symposium on Macromolecules
Place of Presentation
Nagoya, Japan
Year and Date
20070900
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Organization of a bacterial photosynthetic light harvesting complex in supported lipid bilayers2007
Author(s)
T. Takeuchi, R. Sugiura, A. Hiro, T. Down, Y. Suemon, T. Dewa, K. Yamashita, R.Tero, T. Urisu, M. Nango
Organizer
56th SPSJ Annual Meeting
Place of Presentation
Kyoto, Japan
Year and Date
20070500
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Development of novel non viral gene transfer system utilizing polycation liposomes2007
Author(s)
T. Mon, R. Kobayashi, K. Imai, A. Hiro, Y. Tsunoda, T. Dewa, K. Yamashita, T. Asai, N. Oku, M. Nango
Organizer
56th SPSJ Annual Meeting
Place of Presentation
Kyoto, Japan
Year and Date
20070500
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Immobilization and detection of the highest exciton state of the LH2 complex on a quartz or ITO substrate2005
Author(s)
Y. Suemon, K. Iida, K. Shinohara, T. Dewa, H. Sumi, Y. Shibata, S. Itoh, A. T. Gardiner, R. J. Cogdell, M, Nango
Organizer
International Workshop on Photosynthetic Antennae and Coherent Phenomena
Place of Presentation
Osaka, Japan
Year and Date
20051000
Description
「研究成果報告書概要(欧文)」より
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