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2007 Fiscal Year Final Research Report Summary

Development of bio-production process from biogas

Research Project

Project/Area Number 17360401
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionHiroshima University

Principal Investigator

NISHIO Naomichi  Hiroshima University, Graduate School of Advanced Sciences of Matter, Professor (30034383)

Co-Investigator(Kenkyū-buntansha) KAKIZONO Toshihide  Hiroshima University, Graduate School of Advanced Sciences of Matter, Associate Professor (00214255)
Project Period (FY) 2005 – 2007
KeywordsEthanol / Moorella sp / Hydrogen / Carbon dioxide / Carbon monoxide / Biogas / Resting cells / Acetic acid
Research Abstract

(1) Breeding of high ethanol producing Moorella sp. HUC22-1:
To improve the ethanol productivity of Moorella sp. HUC22-1, development of its genetic transformation system was tried. As the results, an uracil auxotroph that lacks dehydrogenase dehydrogenam gene(pyrD) and orotate phosphonlosyltransferaro gene(pyreE) was obtained by the transformation system using homologous recombination and electroporation. Now, we are carrying on a development of genetic transformation system by using the uracil auxotroph, pyrD and pyrE as a host and selection markers.
(2) Analysis of metabolic characterization of Moarella sp. HUC22-1 in various substrates:
Moorella sp. HUC22-1 ferments glyoxylate to acetate roughly according to 2 glyoxylate→acetate 2 CO_2. Based on the data obtained in a batch culture, the ATP yield per substrate dissimilated was calculated to be 1.11 mol/mol, which was higher than that obtained from only Wood-Ljungdahl pathway. Crude extracts of glyoxylate-grown cells catalyzed the ADP- … More and NADP-dependent condensation of glyoxylate and acetyl-CoA to pyruvate and CO_2 as well as the conversion of pyruvate to acetyl-CoA and CO_2. ATP generation was also detected during the key enzyme reactions of this pathway. Furthermore, L-malate, an intermediate in the malyl-CoA pathway, was consumed and acetate was produced in this bacterium. These findings suggest that Moorella sp. HUC22-1 can generate ATP by substrate-level phosphorylation during glyoxylate catabolism through the malyl-CoA pathway. But, ethanol was not produced in all substrates tested including glyoxylate, glycolate, oxalate and malate.
(3) Ethanol production in gaseous substrate in batch culture:
In this year, we used CO as substrate instead of H_2/CO_2. Although the cell could not grow up to 20% CO in the first batch culture, it can grow 100% CO after repeating the acclimatization in the CO gas. At the 100% CO gas substrate, cell growth could increase 8 times and the product could be sifted to ethanol production by increasing the acetate/ethanol ratio.
(4) Conversion of acetate to ethanol in resting cell system:
Conversion of acetate to ethanol was carried out in the resting cell system. As the results, ethanol production was increased to 147 g/kg dry cell under mechanical shaking in the presence of methyl-viologen at pH7. Less

  • Research Products

    (11 results)

All 2008 2007 Other

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Degradation of glyoxylate and glycolate with ATP synthesis by a therm ophilic anaerobic bacterium, Moorella sp. HUC22-1.2008

    • Author(s)
      Shinsuke Sakai
    • Journal Title

      Applied and Environmental Microbiology 74

      Pages: 1447-1452

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Degradation of glyoxylate and glycolate with ATP synthesis by a thermophilic anaerobic bacterium, Moorella sp. HUC22-12008

    • Author(s)
      Shinsuke, Sakai, Kentaro, Inokuma, Yutaka, Nakashimada, Naomichi, Nishio
    • Journal Title

      Applied and Environmental Microbiology 74

      Pages: 1447-1452

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of enzymes involved in the ethanol production of Moorella sp. HUC22-12007

    • Author(s)
      Kentaro Inokuma
    • Journal Title

      Archives of Microbiology 188

      Pages: 37-45

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Recent development of anaerobic digestion processes for energy recovery from wastes2007

    • Author(s)
      Naomichi Nishio
    • Journal Title

      Journal of Bioscience and Bioengineering 103

      Pages: 105-112

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Characterization of enzymes involved in the ethanol production of Moorella sp. HUC22-12007

    • Author(s)
      Kentaro, Inokuma, Yutaka, Nakashimada, Naomichi, Nishio
    • Journal Title

      Archives of Microbiology 188

      Pages: 37-45

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Recent development of anaerobrc digestion processes for energy recovery from wastes2007

    • Author(s)
      Naomichi, Nishio, Yutaka, Nakashimada
    • Journal Title

      Journal of Bioscience and Bioengineering 103

      Pages: 105-112

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Acetate and ethanol production from H_2-co_2 by Moorella sp. HUC22-12007

    • Author(s)
      Kentaro Inokuma
    • Organizer
      Symposium of the International Conference on Biorefinery
    • Place of Presentation
      Beijing (China)
    • Year and Date
      2007-10-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Acetate and ethanol production from H_2-CO_2 by Moorella sp. HUC22-12007

    • Author(s)
      Kentaro, Inokuma, Yutaka, Nakashimada, Shinsuke, Sakai, Naomichi, Nishio
    • Organizer
      Symposium of the International Conference on Bioreftnery
    • Place of Presentation
      Beijing, China
    • Year and Date
      2007-10-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 水素利用技術集成・加速する実用化技術開発(乾式メタン発酵と水素製造、分担執筆)2007

    • Author(s)
      西尾 尚道
    • Total Pages
      11
    • Publisher
      (株)エヌ・ティー・エス
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Hydrogen production and dry methane fermentation,In Collection of hydrogen utilization-accelerating development of applicative technology2007

    • Author(s)
      Naomichi, Nishio, Yutaka, Nakashimada
    • Total Pages
      181-191
    • Publisher
      NTS-book Co
    • Description
      「研究成果報告書概要(欧文)」より
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.hiroshima-u.ac.jp/adsm/bio/biotechnology/index.html

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Published: 2010-02-04  

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