2007 Fiscal Year Final Research Report Summary
Target molecule recognition and redox regulation by the chloroplast thioredoxin
| Project/Area Number |
17370015
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
HISABORI Toru Tokyo Institute of Technology, Chemical Resources Laboratory, Associate Professor (40181094)
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| Co-Investigator(Kenkyū-buntansha) |
NUREKI Osamu Tokyo Institute of Technology, Department of Biological Information, Graduate School of Bioscience and Biotechnology, Professor (10272460)
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| Project Period (FY) |
2005 – 2007
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| Keywords | thioredoxin / redox regulation / disulfide bond / protein-protein interaction / cyanobacteria |
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| Research Abstract |
A. Interaction between thioredoxins and their targets
We first studied the interaction between the plant cytosolic thioredoxin (Trx-hl) and the cytosolic malate dehydrogenase and successfully revealed that this malate dehydrogenase is the newly identified Trx-target protein. We then studied the counterpart proteins of thioredoxins from photosynthetic bacteria and cyanobacteria to reveal the multiplicity of the role of thioredoxins. In addition, we clarified the difference of the roles of two redox-equivalent supply pathways, ferredoxin-dependent thioredoxin reductase pathway and NADPH dependent thioredoxin reductase pathway in the cyanobacteria Synechocystis sp. PCC6803.
B. Structural analysis of the protein-protein interaction between thioredoxins and their targets.
One of the most interesting questions are why thioredoxins can recognize various proteins, which contains the redox sensitive disulfide bond on the molecular surface, as their target. In order to answer this query, we tried to solve the crystal structures of the thioredoxin-target protein cocomplex using X-ray crystallography. Although we have tried to efficiently obtain the co-complexexes under the various chemical conditions, we failed to set up the suitable experimental conditions to obtain the complex proteins during the research term and could not solve any structures which should be important to know the amino acid residues which may involve into the interaction.
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[Journal Article] Binary reducing equivalent pathways using NADPH-thioredoxin reductase and ferredoxin-thioredoxin reductase in the cyanobacterium Synechocystis sp. strain PCC 6803.2008
Author(s)
S, Hishiya, W, Hatakeyama, Y, Mizota, N, Hosoya-Matsuda, K, Motohashi, M, Ikeuchi, T., Hisabori
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Journal Title
Plant Cell Phyisology 49
Pages: 49
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The significance of type II and PrxQ peroxiredoxins for antioxidative stress response in the purple bacterium Rhodobacter sphaeroides2007
Author(s)
M, Wakita, S, Masuda, K, Motohashi, T, Hisabori, H, Ohta, K., Takamiya
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Journal Title
J. Biol. Chem 282
Pages: 27792-27801
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin2007
Author(s)
A, Ikegami, N, Yoshimura K, Motohashi, S, Takahashi, PG, Romano, T, Hisabori, K, Takamiya, T., Masuda
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Journal Title
J. Biol. Chem 282
Pages: 19282-19291
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Thioredoxin affinity chromatography: a useful method for further understanding the thioredoxin network2005
Author(s)
Hisabori, T., Hara, S., Fujii, T., Yamazaki, D., Hosoya-Matusda, N., Motohashi, K.
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Journal Title
Journal of Experimental Botany 56(416)
Pages: 1463-1468
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Thioredoxin affinity chromatography : a useful method for further understanding the thioredoxin network2005
Author(s)
T., Hisabori, S. Hara, T., Fujii, D., Yamazaki, N., Hosoya-Matusda, K., Motohashi
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Journal Title
Journal of Experimental Botany 56
Pages: 1463-1468
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Thioredoxin-hl reduces and reactivates the oxidized cytosolic malate dehydrogenase dimer in higher plants2005
Author(s)
S., Hara, K., Motohashi, F., Arisaka, PG., Romano, N., Hosoya-Matsuda, N., Kikuchi, N., Fusada, T., Hisabori
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Journal Title
J. Biol. Chem 281
Pages: 32065-32071
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Functional analysis of isoforms of CHLI subunit of Mg-chelatase in Arabidopsis thaliana2008
Author(s)
K., Kobayashi, N., Mochizuki, N., Yoshimura, K., Motohashi, T., Hisabori, T., Masuda
Organizer
49th Annual Meeting of the Japanese Society of Plant Physiologist
Place of Presentation
Sapporo
Year and Date
2008-03-19
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Study on the two thioredoxin reduction pathways in Synechocystis sp. PCC6803 by gene disruption.2007
Author(s)
W., Hatakeyama, S., Hishiya, N., Matsuda, K., Motohashi, M., Ikeuchi, T., Hisabori
Organizer
Meeting for cyanobacteria
Place of Presentation
Kazusa, Chiba
Year and Date
2007-12-03
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Two pathways for thioredoxin reduction in Synechocystis sp. PCC68032007
Author(s)
S., Hishiya, Y., Mizota, N., Matsuda, K., Motohashi, M., Ikeuchi, T., Hisabori
Organizer
71th Annual Meeting of the Botanical Society Japan
Place of Presentation
Tokyo Univ. Sci
Year and Date
2007-09-09
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Study of peroxiredoxin of Rhodobacter sphaeroides2007
Author(s)
M., Wakita, S., Masuda, K., Motohashi, T., Hisabori, H., Ohta, K-I., Takamiya
Organizer
48th Annual Meeting of the Japanese Society of Plant Physiologist
Place of Presentation
Ehime Univ
Year and Date
2007-03-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of cysteine residues involved in thioredoxin-dependent redox regulation of CHLI1, a subunit of Mg-chelatase in Arabidopsis thaliana2007
Author(s)
N., Yoshimura, A., Ikegami, K., Motohashi, J., Takahashi, T., Hisabori, T., Masuda
Organizer
48th Annual Meeting of the Japanese Society of Plant Physiologist
Place of Presentation
Ehime Univ
Year and Date
2007-03-28
Description
「研究成果報告書概要(欧文)」より
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