Molecular Mechanism of Cell Wall Integrity Checkpoint

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 17370060
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: OHYA Yoshikazu The University of Tokyo, Graduate School of Frontier Sciences, Integrated Biosciences, Professor (20183767)
• Co-Investigator(Kenkyū-buntansha): SONOIKE Kintake The University of Tokyo, Graduate School of Frontier Sciences, Integrated Biosciences, Associate Professor (30226716)
• Project Period (FY): 2005 – 2006
• Keywords: Cell cycle / checkpoint / dynactin / cell wall / cyclin / spindle

Research Abstract

Cell wall remodeling and cell morphogenesis are tightly coordinated with progression of the cell cycle in many organisms. In the budding yeast, Saccharomyces cerevisiae, a major cell wall component, 1,3-β-glucan is synthesized at the budding site in the early stage of the cell cycle, and constructed in the primary septum before cytokinesis. Yeast possesses both forward and feedback system to achieve temporal and spatial regulation of cell wall construction, Forward system is composed of a cell cycle dependent morphological pathway that is regulated by small GTPase, Rholp. In addition, the feedback regulatory system, called cell wall integrity checkpoint was recently studied and characterized in our laboratory.
We found that yeast cells stop growing at the specific stage of the cell cycle after perturbation of 1,3-β-glucan synthesis. These cells arrest with post-replicative DNA but quite low level of Clb2p, and without forming bipolar spindles. wacl (wall-checkpoint defective) mutation that abolishes this arrest causes the accumulation of C1b2p and CLB2 mRNA and leads to formation of bipolar spindles despite 1,3-β-glucan-synthesis perturbation. These results indicate the existence of a novel cell cycle checkpoint to coordinate entry into mitosis, and suggest that Waclp is required to achieve this checkpoint function through a transcriptional regulation of CLB2.

Research Products

• [Journal Article] Homologous subunit of yeast 1, 3-beta-glucan synthase is important for spore wall rigidity in Saccharomycescerevisiae. (2007)
  • Author(s): Ishihara, S.
  • Journal Title: Eukaryotic Cell 6 Pages: 143-56
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Homologous subunit of yeast 1,3-betaglucan synthase is important for spore wall rigidity in Saccharomyces cerevisiae (2007)
  • Author(s): Ishihara, S., Hirata, A., Nogami, S., Latge, J.-P., Ohya, Y.
  • Journal Title: Eukaryotic Cell 6 Pages: 143-156
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Polo-like kinase Cdc5 controls the local activation of Rhol to promote cytokinesis. (2006)
  • Author(s): Yoshida, S
  • Journal Title: Science 313 Pages: 108-111
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast. (2006)
  • Author(s): Parsons, A. B.
  • Journal Title: Cell 126 Pages: 611-25
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pellman, D. Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. (2006)
  • Author(s): Yoshida, S., Kono, K., Lowery, D. M., Bartolini, S., Yaffe, M. B., Ohya, Y.
  • Journal Title: Science 313 Pages: 108-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Exploring the Mode-of-Action of Bioactive Compounds by Chemical-Genetic Profiling in Yeast. (2006)
  • Author(s): Parsons, A. B., Lopez, A., Givoni, I. E., Williams, D. E., Gray, C. A., Porter, J., Chua, G., Sopko, R., Brost, R. L., Ho, C.-H., Wang, J., Ketela, T., Brenner, C., Brill, J. A., Fernandez, G. E., Lorenz, T. C., Payne, G. S., Ishihara, S., Ohya, Y., Andrews, B., Hughes, T. R., Frey, B. J., Graham, T. R., Andersen, R. J., Boone, C.
  • Journal Title: Cell 126 Pages: 611-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular dissection of ARPl regions required for nuclearmigration and cell wall integri ty checkpoint functionsin Saccharomyces cerevisiae. (2005)
  • Author(s): Igarashi, R.
  • Journal Title: Cell Struct Funct. 30 Pages: 57-67
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Involvement of actin and polarisome in morphologicalchange during spore germination of Saccharomcescerevisiae. (2005)
  • Author(s): Kono, K.
  • Journal Title: Yeast 30 Pages: 129-139
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] High-dimensional and large-scale phenotyping of yeastmutants. (2005)
  • Author(s): Ohya, Y.
  • Journal Title: Proc Natl Acad Sci USA. 102 Pages: 19015-20
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae. (2005)
  • Author(s): Igarashi, R., Suzuki, M., Nogami, S., Ohya, Y.
  • Journal Title: Cell Struct Funct. 30 Pages: 57-67
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] (2005) Involvement of act in and polarisome in morphological change during spore germination of Saccharomyces cerevisiae. (2005)
  • Author(s): Kono, K., Matsunaga, R., Hirata, A., Suzuki, G., Abe, M., Ohya, Y.
  • Journal Title: Yeast 30 Pages: 129-139
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-dimensional and large-scale phenotyping of yeast mutants. (2005)
  • Author(s): Ohya, Y., Sese, J., Yukawa, M., Sano, F., Nakatani, Y., Saito, TL., Saka. A., Fukuda, T., Ishihara, S., Oka, S., Suzuki, G., Watanabe, M., Hirata, A., Ohtani, M., Sawai, H., Fraysse, N., Latge, JP., Francois, JM., Aebi, M., Tanaka, S., Muramatsu, S., Araki, H., Sonoike, K., Nogami, S., Morishita, S.
  • Journal Title: Proc Natl Acad Sci USA. 102(52) Pages: 19015-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 実験医学増刊号Vol. 23 「出芽酵母の細胞壁合成をモニターする新規チェックポイント機構」(分担執筆) (2007)
  • Author(s): 関谷瑞穂
  • Total Pages: 5
  • Publisher: 羊土社
  • Description: 「研究成果報告書概要(和文)」より