2007 Fiscal Year Final Research Report Summary
Molecular genetic dissection of anovel unilateral incompatibility within species, which observed in self-incompatible Brassica species
| Project/Area Number |
17380001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Tohoku University |
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Principal Investigator |
WATANABE Masao Tohoku University, Tohoku University, Graduate School of Life Sciences, Professor (90240522)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Go Osaka Kyoiku University, Faculty of Education, Associate Professor (10314444)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Brassica species / Self-incompatibility / Unilateral incompatibility / Pollen-stigma interaction / Genetic map / Molecular marker / pollen factor of UI / stigma factor of UI |
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| Research Abstract |
Self-incompatibility (SI) systems prevent self-fertilization and promote out-crossing by rejecting pollen from plants with the same Shaplotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, termed S locus. On the pollen-stigma interaction of Brassica species, although the recognition system between species should be existed, the recognition molecules between species are unknown. In the course of genetic analysis of SI in Brassica species, we observed a novel unilateral incompatibility (UI) in Brassica rapa L. This UI was specifically observed when one cultivar (Osome) was used a female partner, and Turkish lines were used as a pollen partner. From genetic analysis, this US locus was independent to S locus. This research purpose is molecular cloning of this UI gene in B. rapa.
From more deep genetic analysis, pollen UI (PUI) phenotype was controlled by a single recessive gene, and stigma UI (SUI) phenotype was controlled by a single dominant gene. For isolation of PUI gene, AFLP analysis was performed with F2 segregation population. As a result, six linking molecular markers were isolated, and were scattered within 18cM on the genetic map. From the comparison of genetic map and UI phenotype, we performed QTL analysis. In the analysis, PUI was located between E-AAC/M-CCT and E-ACG/M-GAC markers (5cM). By using these markers, we will isolate the PUI gene, near future.
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