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2007 Fiscal Year Final Research Report Summary

Clarification of molecular mechanism of rice mutant which shows non-Mendelian Inheritance induced by tissue culture

Research Project

Project/Area Number 17380004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKobe University

Principal Investigator

IMAISHI Hiromasa  Kobe University, Functional Analysis of Environmental Genes Research Center for Environmental Genomics, Professor (50223318)

Co-Investigator(Kenkyū-buntansha) INUI Hideyuki  Kobe University, Functional Analysis of Environmental Genes Research Center for Environmental Genomics, Assistant Professor (90314509)
Project Period (FY) 2005 – 2007
KeywordsRice / P450 / mutant / mendelian
Research Abstract

Plant cell culture often induces genetic variability in cultured tissues as well as in regenerated plants, which includes alterations in gross morphology, maturity, yield, and disease response. Furthermore, the passage of plant tissues through a culture system in vitro can induce several changes at the chromosomal level, which are in the number of chromosomes, in the extent of DNA methylation, and in the copy number of the genomes. In order to clarification of molecular mechanism of rice mutants which shows non-Mendelian Inheritance, we tried to analyze the gene expression levels of rice plant by using DNA-microarray and protein expression system. Rice has about 420 P450 genes which can make plant hormone such as brassinolides and gibberellic acids. P450 isolated from the rice Oryza sativa. The CYP72A18 cDNA was cloned from rice and heterologously expressed in Saccharomyces cerevisiae AH22 cells from the alcohol dehydrogenase (ADHI) promoter. Microsomes isolated from recombinant yeast cells contained the CYP72A18, which was found to catalyze the (ω-1)-hydroxylation of the herbicide pelargonic acid. We also show that (ω-1)-hydroxypelargonic acid has reduced herbicide activity against rice seedlings. Based on these results, we suggest that CYP72A18 participates in the detoxification of the herbicide pelargonic acid in rice plants

  • Research Products

    (4 results)

All 2008 2007

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Isolation and functional characterization in yeast of CYP72A18, a rice cytochrome P450 that catalyzes(ω-1)-hydroxylation of the herbicide pelar gonic acid2007

    • Author(s)
      Imaishi, H., and Matumoto, S.
    • Journal Title

      Pestic. Biochem. Physiol. 88

      Pages: 71-77

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Isolation and functional characterization in yeast of CYP72A18, a rice cytochrome P450 that catalyzes(ω-1)-hydroxylation of the herbicide pelargonic acid2007

    • Author(s)
      Imaishi, H., Matumoto, S
    • Journal Title

      Pestle. Biochem. Physiol 88

      Pages: 71-77

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] カセットプラスミドを用いたヒトP450酵素タンパク質の大腸菌内安定発現とその酵素化学的性質2008

    • Author(s)
      森内寛、付学軍、宇野知秀、一色邦夫、廣末慎嗣、今石浩正
    • Organizer
      日本農芸化学会関西支部第453回講演会
    • Place of Presentation
      京大会館、京都
    • Year and Date
      2008-02-02
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Rice Biotechnology 50 Years-From Embryo Culture to Small RNA2007

    • Author(s)
      K. Oono
    • Organizer
      Bio-Hanoi2007: From Bioscience to Biotechnology and Bioindustry
    • Year and Date
      20071218-19
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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