2007 Fiscal Year Final Research Report Summary
Physiological and molecular biological analysis of some factors related to fit softening and storability
| Project/Area Number |
17380023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Shimane University |
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Principal Investigator |
ITAMURA Hiroyuki Shimane University, Faculty of Life and Environmental Science, Professor (80109040)
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| Co-Investigator(Kenkyū-buntansha) |
AKAURA Kazuyuki University of Shimane, Matsue College, Associated Professor (80144343)
NAKAGAWA Tsuyoshi Shimane University, Center for Integrated Research in Science, Professor (30202211)
ITAI Akihiro Tottori University, Faculty of Agriculture, Associated Professor (10252876)
NAKATSUKA Akira Shimane University, Faculty of Life and Environmental Science, Associated Professor (40304258)
BAN Takuya Shimane University, Faculty of Life and Environmental Science, Associated Professor (20325046)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Fruit softening / on tree ripening / Expressed sequence tag / Magnetic resonance imagine / Plant hormone |
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| Research Abstract |
To obtain the basic information for improving fruit storability, we investigated fruit development and ripening on a tree, and softening after harvest by physiological, histochemical and molecular biological approach.
A total of 15680 expressed sequence tags were generated from randomly selected clones of three different cDNA libraries in 'Saijo' persimmon; fruit at an early stage of development, ripening fruit, and rapidly softened fruit on a tree. We successfully obtained a set of genes that are expressed during fruit development and ripening in persimmon. We have obtained a full length and a partial cDNA encoding arabinofuranosidase and xylosidase/arabinofuranosidase, respectively from persimmon cDNA library. The higher expression of both genes were in accordance with rapid softening. This result indicates that both genes may play an important role in softening after artificial de-astringency.
As the treatment of 1-methylcyclopropene, ethylene action inhibitor; did not affect fruit coloration in 'Saijo' persimmon fruit on a tree, spontaneously and transiently produced internal ethylene of the fruit on a tree is unrelated to coloration on a tree. On the other hand, fruit softening like a jerry in late autumn on a tree is corresponding to increase of internal ethylene. In grape berries and blueberry fruit, the changes in the inner contents were investigated during the growth and ripening period. It is cleared that the growth rate of grape berries is quite different from each part of tissue and the cell wall contents of blueberry fruit are different among the picking times.
Clear Magnetic Resonance Imaging (MRI) images were obtained in softening 'Saijo' Japanese persimmon fruit witch was treated with ethylene. Nondestructive method, such as MRI imaging, enabled internal observation of very soft persimmon fruit. Infiltration of fruit tissue seemed to begin around vascular bundles of fruit. Loosening of tissue was observed by photo-microscopy in softening fruit.
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[Journal Article] Expressed sequence tags (ESTs) from persimmon at different developmental stages.2008
Author(s)
Nakagawa, T., A., Nakatsuka, K., Yano, S., Yasugahira, K., Nakamura, N., Sun, A., Itai, T., Suzuki, H., Itamura
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Journal Title
Plant Cell Report (In press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effects of girdling, defoliation and flooding treatments on fruit softening before and after harvest in 'Saijo' persimmon (Diospyros kaki Thunb.)2007
Author(s)
Matsumoto, T., H., Itamura, T., Kurahashi, S., maxi, S., Matsumoto
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Journal Title
Hort. Res. (Japan) 6
Pages: 119-123
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Determination of the ethylene synthetic genotypes related to fruit ripening in Japanese pear cultivars2005
Author(s)
Itai, A., T., Kotaki, K., Tanabe, M., Fukuda, Y., Kawada, Y., Amano, N., Fujita
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Journal Title
J. Japan. Soc. Hort. Sci 74
Pages: 361-366
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The relationship between coloring and internal ethylene in persimmon fruit on a tree2008
Author(s)
Itamura, H., Y., Hanaoka, T., Nakamoto, S., Naritoku, N., Sun
Organizer
Spring meeting of Japanese Society for Horticultural Science
Place of Presentation
Tokyo University of Agriculture, Japan
Year and Date
20080328-29
Description
「研究成果報告書概要(欧文)」より
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[Presentation] EST analysis of cDNA libraries from persimmon fruit during development and ripening2008
Author(s)
Nakatsuka, A., T., Nakagawa, K., Yann, A., Ttai, N., Kobayashi, H., Itamura
Organizer
Spring meeting of Japanese Society for Breeding
Place of Presentation
Meiji University, Japan
Year and Date
20080327-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Short storability after removal of astringency by dry-ice treatment in various cultivars and some trials of storability improvement in persimmon2007
Author(s)
Itamura, H., H., Sun, C., Xu, A., Nakatsuka, Y., Hanaoka, S., Naritoku, T., Nakamoto, K., Akaura
Organizer
Europe-Asia Symposium on Quality Management in Postharvest Systems
Place of Presentation
Bangkok, Thailand
Year and Date
20071203-06
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of wound treatment to fruit on a tree on ethylene induction and the role of leaves2007
Author(s)
Sun, N., Y., Hanaoka, S., Naritoku, A., Nakatsuka, H., Itamura
Organizer
Spring meeting of Japanese Society for Horticultural Science
Place of Presentation
Kyoto, Japan
Year and Date
20070324-25
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expression analysis of the pyruvate decarboxylase gene (DkPDC2) involved in natural de-astringency of pollination variant cultivars in persimmon2007
Author(s)
Itai, A., M., Kawakami, M., Yamada, K., Tanabe, H., Itamura, T., Kadowaki
Organizer
Spring meeting of Japanese Society for Horticultural Science
Place of Presentation
Kyoto, Japan
Year and Date
20070324-25
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 果実のエチレン生成と保存.2007
Author(s)
花岡陽介・孫 寧静・中務 明・板村裕之.
Organizer
園芸学会中四国支部会
Place of Presentation
島根大学
Year and Date
2007-07-28
Description
「研究成果報告書概要(和文)」より
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[Presentation] Ethylene production and preservation of fruit2007
Author(s)
Hanaoka, Y., N., Sun, A., Nakatsuka, H., Itamura
Organizer
Annual meeting of Japanese Society for Horticultural Science, branch office of Chugoku-Shikoku
Place of Presentation
Shimane University, Japan
Year and Date
2007-07-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] It is wrong that ethanol converts to acetaldehyde which removes astringency of persimmon fruit by ethanol treatment2006
Author(s)
Kawakami, M., A., Itai, H., Itamura, K., Tanabe, M., Yamada, T., Kdowaki
Organizer
Autumn meeting of Japanese Society for Horticultural Science
Place of Presentation
Nagasaki University, Japan
Year and Date
20060923-26
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expressed sequence tags from persimmon at different developmental staees2006
Author(s)
Nakatsuka, A., T., Nakagawa, S., Yasugahira, R., Nakamura, N., Sun, A., Itai, T., Suzuki, H., Itamura
Organizer
27th International Horticultural Congress
Place of Presentation
Seoul, Korea
Year and Date
20060813-19
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Effects of girdling, defoliation and flooding treatments on fruit softening before and after harvest in 'Saijo' persimmon (Diospyros kaki Thunb.)2006
Author(s)
Matsumoto, T., H., Itamura, T., Kurahashi, S., Maki, S., Matsumoto
Organizer
Annual meeting of Japan Association of Food Preservation Science
Place of Presentation
Kobe University Japan
Year and Date
20060623-24
Description
「研究成果報告書概要(欧文)」より
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