2007 Fiscal Year Final Research Report Summary
Molecular characterization and role of ecdysone membrane receptor
| Project/Area Number |
17380035
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kanazawa University |
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Principal Investigator |
SAKURAI Sho Kanazawa University, Graduate School of Natural Science and Technology, Professor (80143874)
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| Co-Investigator(Kenkyū-buntansha) |
IWAMI Masafumi Kanazawa University, Graduate School of Natural Science and Technology, Professor (40193768)
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| Project Period (FY) |
2005 – 2007
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| Keywords | silk worm / Bombyx mori / programmed cell death / 20-hydroxyecdysone / sienal transduction |
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| Research Abstract |
We clarified developmental profiles of gene expression of early response genes to 20E in the anterior silk glands during the fifth instar up to the time of cell death execution two days after gut purge. Also, we showed the gene response to 20E in vitro using anterior silk glands of gut-purged larvae. The in vivo and in vitro results indicated that a heterodimeric EcR-B1 and USP-2 may be responsible for the cell death Results also indicated involvement of E74, E75, BHR3 and BR-C isoforms, but not Ftz-F1. We are not succeeded in gene cloning of the putative membrane ecdysone receptor yet. We examined pharmacologically the signaling pathway from mEcR to cellular responses, i.e. cell condensation, nuclear condensation, DNA fragmentation and nuclear fragmentation. Ca<2+> acts as the second messenger The mEcR is suggested to be a G-protein coupled receptor (GPCR) associated with Gαq, followed by a serial activation of phospholipase c-β, generation of inositol 3-phosphate (IP_3), and release of Ca<2+> from endoplasmic reticulum probably through IP3 receptor Then, Ca<2+> activates protein kinase C (PKC) and caspase 3-like protease. This signaling pathway culminates in nuclear fragmentation and nuclear fragmentation. Nuclear condensation is regulated by a different pathway involving calmodulin and calmodulin-dependent protein kinase II (CaMK-II). However, this pathway was not activated by Ca<2+>, and therefore it is unknown whether Gαq is involved in this pathway. In addition, inhibitors of calmodulin and CaMK-II affected the occurrence of nuclear and DNA fragmentations, indicating the caspase 3-like protease activation does not depend simply on the signaling pathway of GPCR/PLC-β/IP3/Ca<2+>/PKC.
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[Journal Article] Identification, characterization, and developmental regulation of two storage proteins in the bamboo borer Omphisa fuscidentalis2007
Author(s)
Tungjitwitayalkul, T., Singtripop ; T., Nettagul ; A., Oda,. Y., Tatun ; N., Sekimoto ; T., and Sakurai, S
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Journal Title
J. Insect Physiol 54
Pages: 62-76
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Correlation of oxygen consumption, cytochrome c oxidase and cytochrome c oxidase subunit I gene expression in the termination of larval diapause in the bamboo borer, Omphisa fuscidentalis2007
Author(s)
Singtripop, T., Saeangsakda, M., Tatun, N., Kaneko, Y., Sakurai, S
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Journal Title
J. Insect Physiol 53
Pages: 933-939
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 20-Hydroxyecclysone- (20E-) induced genes expression in the brain and their functional analysis by RNAi during development of the silkworm, Bombyx mart2007
Author(s)
Monwar, Hossain, Sakiko, Shimizu, Masahiro, Matsuki, Masanori, Imamura, Sho, Sakurai, Masafumi, Iwami
Organizer
30th Japan Molecular Biology Society Annual Meeting
Place of Presentation
Yokohama
Year and Date
20071211-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Spatial distribution of 20-hydroxyecdysone (20E)-responsive genes in the brain of silkworm, Bombyx mori2007
Author(s)
Anuradha, Roy, Sakiko, Shimizu, Sho, Sakurai, Kazuei, Mita, Masafumi, Iwami
Organizer
30th Japan Molecular Biology Society Annual Meeting
Place of Presentation
Yokohama
Year and Date
20071211-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genomic and nongenomic actions of an insect steroid, 20-hydroxyecdysone in programmed cell death of Bombyx anterior silk gland2007
Author(s)
Manaboon, M., Iga, M., Sekimoto, M., Elmogy, M., Iwami, M., Sakurai, S
Organizer
Sixth Asia and Oceania Society for Comparative Endocrinology
Place of Presentation
Bagdogra, India
Year and Date
20071210-14
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification and characterization of two storage proteins in diapause larvae of the bamboo borer, Omphisa fuscidentalis2006
Author(s)
Tungjitwitayakul, J., Oda, Y., Tatun N., Kaneko, Y., Sekimoto, T., Singtripop, T., Sakurai, S
Organizer
7th International Workshop on the Molecular Biology and Genetics of the Lepidoptera
Place of Presentation
Kolympari, Crete, Greece
Year and Date
20060820-26
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Interaction of genomic and nongenomic actions of 20-hydroxyecdysone in 20E-dependent developmental events2006
Author(s)
Iga, M., Sekimoto, M., Elmogy, M., Iwami, M., Sakurai, S
Organizer
XVIth International Ecdysone Workshop
Place of Presentation
Ghent, Bergium
Year and Date
20060710-14
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Comprehensive analysis of gene expression induced by 20-hydroxyecdysone (20E) in the silkworm brain : Analysis with microarray2006
Author(s)
Sakiko, Shimizu, Monwar, Hossain, Yu, Kaneko, Sho, Sakurai, Hiroaki,Noda, Kazuei Mita, Masafumi, Iwami
Organizer
20th IUBMB International Congress of Biochemistry and Molecular Biology
Place of Presentation
Kyoto, Japan
Year and Date
20060618-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Expression and functional analysis of the 20-hydroxyecdysone (20E) -induced brain genes during development of the silkworm Bombyx mart2006
Author(s)
Monwar, Hossain, Sakiko, Shimizu, Masahiro, Matsuki, Masanori, Imamura, Sho, Sakurai, Masafumi, Iwami
Organizer
Japan Molecular Biology Society
Place of Presentation
Fromm
Year and Date
20060000
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Comprehensive analysis of gene expression induced by 20-hydroxyeadysone (20E) in the silkworm brain : Identification of two novel genes2005
Author(s)
Md. Monwar, Hossain, Sakiko, Shimizu, Yu, Kaneko, Masahiro, Matsuki,Masanori, Imamura, Sho, Sakurai, Masafumi, Iwami
Organizer
28th Japan Molecular Biology Society Annual Meeting
Place of Presentation
Fukuoka, Japan
Year and Date
20051207-10
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genomic and nongenomic actions of 20E in programmed cell death of Bombyx anterior silk gland2005
Author(s)
Iga, M., Sekimoto, T., Elmogy, M., Iwami, M., Sakurai, S
Organizer
The Fifth Asia-Pacific Congress of Entomology
Place of Presentation
Jeju, South Korea
Year and Date
20051018-21
Description
「研究成果報告書概要(欧文)」より
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