Protein engineering of thermolysin and reaction-control engineering for the aspartame synthesis using thermolysin

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 17380065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: INOUYE Kuniyo Kyoto University, Graduate School of Agriculture, Professor (10223249)
• Co-Investigator(Kenkyū-buntansha): YASUKAWA Kiyoshi Kyoto University, Graduate School of Agriculture, Associate Professor (30397559) ; TAKITA Teisuke Kyoto University, Graduate School of Agriculture, Assistant Professor (70263126)
• Project Period (FY): 2005 – 2007
• Keywords: enzyme / Protein engineering / reaction control / Peptide / food / protease / thermolysin / site-directed mutagenesis

Research Abstract

Thermolysin (TLN) is a metalloproteinase with high activity and a thermophilic and halophilic enzyme. The activity increases exponentially with salt and does 13 times with 4 M NaCl. It is utilized for aspartame synthesis, and its Stabilization and activation are desired.
1. Ser residues located on the TLN surface were converted to Asp individually. The resulting variants were as active as the wild-type enzyme (WT), and more halophilic than WT. For the variants S53D and S65D, in which Ser53 and Ser65 were changed to Asp, respectively, the thermostability was much improved at 10 mM Ca^<2+>, but, the WT stability at 100 mM Ca^<2+> was similar to that of the variants, suggesting that the variants enhanced the stability at 10 mM Ca^<2+> with higher affinity of Ca^<2+> to Asp than Ser.
2. The variant G8C/N60C/S65P/L144S was activated and stabilized 5-10 times higher than WT. This stabilization is given by summation of the effects due to 4 single mutations.
3. The auto-cleavage site was identified as G1y154-Leu155. The variant L155A was 4 times stable than WT at 90℃.
4. The X-ray crystallographic study of TLN at 4 M NaCl showed that N-domain of TLN moved 1.5 Angstrom far from C-domain on adding 4 M NaCl and shifted to the open-form, which might be related to the higher activity observed at high salt concentrations.
5. A mutant strain producing TLN variant A73V was isolated. It showed higher activity to peptide substrate than WT, and also showed higher performance in aspartame synthesis than WT.
As shown above, we designed, expressed, and characterized TLN variants which could be potentially utilized for aspartame synthesis.

Research Products

• [Journal Article] Comparison of the thermal stabilities of reverse transcriptases from avbian myeloblastosis virus and moloney murine leukemia virus. (2008)
  • Author(s): Yasukawa, K., Nemoto, D., and Inouye, K.
  • Journal Title: J. Biochem. 143(2) Pages: 261-268
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase. (2008)
  • Author(s): Kojima, K., Tsuzuki, S., Fushiki, T., and Inouye, K.
  • Journal Title: J. Biol. Chem 283(5) Pages: 2478-2487
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability. (2008)
  • Author(s): Takita, T., Aono, T., Sakurama, H., Itoh, T., Wada, T., Minoda, M., Yasukawa, K., and Inouye, K.
  • Journal Title: Biochim. Biophys. Acta 1784(3) Pages: 481-488
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Purification and characterization of novel raw-starch-digesting and cold-adapted α-amylases from Eisenia foetida. (2008)
  • Author(s): Ueda, M., Asano, T., Nakazawa, M., Miyatake, K., and Inouye, K.
  • Journal Title: Compt. Biochem. Physiol. B150 Pages: 125-130
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Gene cloning, expression, purification and characterization of rice(Oryza sativa L.)class II chitinase CHT11. (2008)
  • Author(s): Xayphakatsa, K. Tsukiyama, T., Inouye, K., Okumoto, Y., Nakazaki, T., and Tanisaka, T.
  • Journal Title: Enz. Microb. Technol (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Parasporin-4, a novel cancer cell-killing protein produced by Bacillus thuringiensis(Minireview). (2008)
  • Author(s): Inouye, K., Okumura, S., and Mizuki, E.
  • Journal Title: Food Sci. Biotechnol. 17(2) Pages: 219-227
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comparison of the thermal stabilities of reverse transcriptases from avbian myeloblastosis virus and moloney murine leukemia virus (2008)
  • Author(s): Yasukawa, K., Nemoto, D., Inouye, K
  • Journal Title: J. Biochem 143(2) Pages: 261-268
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase (2008)
  • Author(s): Kojima, K., Tsuzuki, S., Fushiki, T., Inouye, K
  • Journal Title: J Biol. Chem 283(5) Pages: 2478-2487
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability (2008)
  • Author(s): Takita, T., Aono, T., Sakurama, H., Itoh, T., Wada, T., Minoda, M., Yasukawa, K., Inouye, K
  • Journal Title: Biochim. Biophys. Acta 1784(3) Pages: 481-488
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Purification and characterization of novel raw-starch-digesting and cold-adapted a-amylases from Eivenia foetida (2008)
  • Author(s): Takita, T., Aono, T., Sakurama, H., Itoh, T., Wada, T., Minoda, M., Yasukawa, K., Inouye, KUeda, M., Asano, T., Nalcazawa, M., Miyatake, K., Inouye, K
  • Journal Title: Compt. Biochem. Physioi 150 Pages: 125-130
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Gene cloning, expression, purification and characterization of rice (Oryza sativa L.) class II chitinase CHT11 (2008)
  • Author(s): Xayphakatsa, K. Tsukiyama, T., Inouye, K., Okumoto, Y., Nakazaki, T., Tanisaka, T
  • Journal Title: Enz. Microb. Technol (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Parasporin-4, a novel cancer cell-killing protein produced by Bacillus thuringiensis (Minireview) (2008)
  • Author(s): Inouye, K., Okumura, S., Mizuki, E
  • Journal Title: Food Sof. Biotechnol 17(2) Pages: 219-227
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Reaction mechanism of thermolysin (2008)
  • Author(s): Inouye, K., Kamo, M
  • Journal Title: Structural Biology ; Introduction and Background (Ed. by Kawano, K. and Tanokura, M.) Kyoritsu Shuppan 91-98 Pages: 203
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enzyme Handbook, 3rd Edition (Ed. by Yagi, T., Fukui, T., Ichishima, E., Kagamiyama, H., and Toraya, T.) (2008)
  • Author(s): Inouye, K (coauthor)
  • Journal Title: Asakura Shoten Pages: 1,008
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification and characterization of a novel cytotoxic protein, parasporin-4, produced by Bacillus thuringiensis A 1470 strain (2008)
  • Author(s): Okumura, S., Saitoh, H., Ishikawa, T., Mizuki, E., Inouye, K
  • Journal Title: Biotechnol. Annu (in press) Pages: 14
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of neutral salts and alcohols on the activity of Streptomyces caespitosus neutral protease. (2007)
  • Author(s): Inouye, K., Shimada, T., and Yasukawa, K.
  • Journal Title: J. Biochem. 142(3) Pages: 317-324
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effects of site-directed mutagenesis of the surface residues Gln128 and Gln225 0f thermolysin on its catalytic activity. (2007)
  • Author(s): Tatsumi, C., Hashida, Y., Yasukawa, K., and Inouye, K.
  • Journal Title: J. Biochem. 141(6) Pages: 835-842
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Kinetic analysis of the activation-and- inhibition dual effects of cobalt ion on thermolysin activity. (2007)
  • Author(s): Hashida, Y. and Inouye, K.
  • Journal Title: J. Biochem. 141(6) Pages: 843-853
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular mechanism of the inhibitory effect of cobalt ion on thermolysin activity and the suppressive effect of calcium ion on the cobalt ion-dependent inactivation of thermolysin. (2007)
  • Author(s): Hashida, Y. and Inouye, K.
  • Journal Title: J. Biochem. 141(6) Pages: 879-888
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Purification to homogeneity of a neutral metalloproteinase from Streptomyces caespitousus. (2007)
  • Author(s): Inouye, K., Shimada, T., and Yasukawa, K.
  • Journal Title: Biosci. Biotechnol. Biochem. 71(7) Pages: 1773-1776
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Role of Gln85 of human CYP27A1 in 25-hydroxyvitamin D3-biinding and protein folding. (2007)
  • Author(s): Sawada, N., Yamamoto, K., Yamada, S., Ikushiro, S., Kamakura, M., Ohta, M., Inouye, K., and Sakaki, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 355(1) Pages: 211-216
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Improvement in performances of affinity gels containing Gly-D-Phe as a ligand to thermolysin by increasing the spacer chain length. (2007)
  • Author(s): Inouye, K., Nakamura, K., Kusano, M., and Yasukawa, K.
  • Journal Title: Biosci. Biotechnol. Biochem. 71(8) Pages: 2083-2086
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A new method for the extracellular production of recommbinant thermolysin by co-expression of the mature sequence and the prosequence in Escherichia coli. (2007)
  • Author(s): Yasukawa, K., Kusano, M., and Inouye, K.
  • Journal Title: Protein Eng. Des. Sel. 20(8) Pages: 375-383
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Improving the activity and stability of thermolysin by site-directed mutagenesis (2007)
  • Author(s): Yasukawa, K., Inouye, K
  • Journal Title: Biochim. Biophys.Acta 1774(10) Pages: 1281-1288
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of neutral salts and alcohols on the activity of Streptomyces caespitosus neutral protease (2007)
  • Author(s): Inouye, K., Shimada, T., Yasukawa, K
  • Journal Title: J. Biochem 142(3) Pages: 317-324
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effects of site-directed mutagenesis of the surface residues GIn128 and G1n225 of thermolysin on its catalytic activity (2007)
  • Author(s): Tatsumi, C., Hashida, Y., Yasukawa, K, Inouye, K
  • Journal Title: Biochem 141(6) Pages: 835-842
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity (2007)
  • Author(s): Hashida, Y., Inouye, K
  • Journal Title: J. Biochem 141(6) Pages: 843-853
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular mechanism of the inhibitory effect of cobalt ion on thermolysin activity and the suppressive effect of calcium ion on the cobalt ion-dependent inactivation of thermolysin (2007)
  • Author(s): Hashida, Y., Inouye, K
  • Journal Title: J. Biochem 141(6) Pages: 879-888
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Purification to homogeneity of a neutral metalloproteinase from Streptomyces caespitousus (2007)
  • Author(s): Inouye, K., Shimada, T., Yasukawa, K
  • Journal Title: Biosci. Biotechnol. Biochem 71(7) Pages: 1773-1776
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of Gln85 of human CYP27A1 in 25-hydroxyvitamin D3-binding and protein folding (2007)
  • Author(s): Sawada, N., Yamamoto, K., Yamada, S., Ikushiro, S., Kamakura, M., Ohta, M., Inouye, K., Sakaki, T
  • Journal Title: Biochem. Biophys. Res. Commun 355(1) Pages: 211-216
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Improvement in performances of affinity gels containing Gly-D-Phe as a ligand to thermolysin by increasing the spacer chain length (2007)
  • Author(s): Inouye, K., Nakamura, K., Kusano, M., Yasukawa, K
  • Journal Title: Biosci. Biotechnol. Biochem 71(8) Pages: 2083-2086
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anew method for the extracellular production of recommbinant thermolysin by co-expression of the mature sequence and the prosequence in Escherichia coil (2007)
  • Author(s): Yasukawa, K., Kusano, M., Inouye, K
  • Journal Title: Protein Eng. Des. Sel 20(8) Pages: 375-383
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Engineering, expression, purification, and production of recombinant thermolysin (2007)
  • Author(s): Inouye, K., Kusano, M., Hashida, Y., Minoda, M., Yasukawa, K
  • Journal Title: Biotechnol Annu 13 Pages: 43-65
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Biotechnology : Recent development of chromatographic technology and its application to therapy and diagnosis (S26) (2007)
  • Author(s): Inouye, K
  • Journal Title: 20th IUBMB International Congress of Biochemistry and Molecular Biology Reports (Eds., Congre Co. ; Taniguchi, N. and Suzuki. K.) 65
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Dissociation/association properties of a dodecameric cyclomaltodextrinase. Effects of pH and salt concentration on the oligomeric state. (2006)
  • Author(s): Lee, H.-S., Kim, J.-S., Shim, K., Kim, J.-W., Inouye, K., Oneda, H., Kim, Y.-W., Cheong, K.-A., Cha, H., Woo, E.-J., Auh, J. H., Lee, S.-J., Kim, J.-W., and Park, K.-H.
  • Journal Title: FEBS J. 273 Pages: 109-121
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic chara cterization. (2006)
  • Author(s): Inouye, K., Minoda, M., Takita, T., Sakurama, H., Hashida, Y., Kusano, M., and Yasukawa, K.
  • Journal Title: Protein Expr. Purif. 46 Pages: 248-255
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin. (2006)
  • Author(s): Yasukawa, K., Kusano, M., Nakamura, K., and Inouye, K.
  • Journal Title: Protein Expr. Purif. 46 Pages: 332-336
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase genefamily in rice(Oryza sativa L.). (2006)
  • Author(s): Nakazaki, T., Tsukiyama, T., Okumoto, Y., Kageyama, D., Naito, K., Inouye, K., and Tanisaka, T.
  • Journal Title: Genome 49(6) Pages: 619-630
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Purification and some properties of a D-fructose-1, 6-bisphosphate aldolase from red alga, Gracilaria chorde Holmes. (2006)
  • Author(s): Kakita, H., Kamishima, H., and Inouye, K.
  • Journal Title: Botanica Marina 49(2) Pages: 174-181
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Structure-function analysis of vitamin D 24-hydroxylase(CYP24A1)by site-directed mutagenesis: amino acid residues responsible for species-based difference of CYP24A1 between humans and rats. (2006)
  • Author(s): Hamamoto, H., Kusudo, T., Urushino, N., Masuno, H., Yamamoto, K., Yamada, S., Kamakura, M., Ohta, M., Inouye, K., and Sakaki, T.
  • Journal Title: Mol. Pharmacol. 70(1) Pages: 120-128
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interaction between mitochondrial CYP27B1 and adrenodoxin: Role of arginine 458 of mouse CYP27B1. (2006)
  • Author(s): Urushino, N., Yamamoto, K. Kagawa, N., Ikushiro, S., Kamakura, M., Yamada, S., Kato, S., Inouye, K., and Sakaki, T.
  • Journal Title: Biochemistry 45(14) Pages: 4405-4412
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Engineering of the pH-dependence of thermolysin activity asexamined by site-directed mutagenesis of Asn112 located at the active site of thermolysin. (2006)
  • Author(s): Kusano, M., Yasukawa, K., Hashida, Y., and Inouye, K.
  • Journal Title: J. Biochem. 139(6) Pages: 1017-1023
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis α-amylases and insights into engineering α-amylase variants active at acidic conditions. (2006)
  • Author(s): Lee, S., Oneda, H., Minoda, M., Tanaka, A., and Inouye, K.
  • Journal Title: J. Biochem. 139(6) Pages: 997-1005
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Comparison of the wild-type α-amylase and its variant enzymes of Bacillus amyloliquefaciens in their activity and thermal stability, and insights into engineering the thermal stability of Bacillus α-amylase. (2006)
  • Author(s): Lee, S., Mouri, Y., Minoda, M., Oneda, H., and Inouye, K.
  • Journal Title: J. Biochem. 139(6) Pages: 1007-1015
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Efficient solubilization, activation, and purification of recombinant Cry45Aa of Bacillus thuringiensis expressed as inclusion bodies in Escherichia coli. (2006)
  • Author(s): Okumura, S., Saitoh, H., Wasano, N., Katayama, H., Higuchi, K., Mizuki, E., and Inouye, K.
  • Journal Title: Protein Expr. Purif. 47(1) Pages: 144-151
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of high concentration of salts on the esterase activity and structure of kiwifruit peptidase, actinidain. (2006)
  • Author(s): Morimoto, K., Furuta, E., Hashimoto, H., and Inouye, K.
  • Journal Title: J. Biochem. 139(6) Pages: 1065-1071
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Enzymatic properties of human CYP2W1 expressed in Escherichia coli. (2006)
  • Author(s): Yoshioka, H., Kasai, N., Ikushiro, S., Shinkyo, R., Kamakura, M., Ohta, M. Inouye, K., and Sakaki, T.
  • Journal Title: Biochem. Biophys. Res. Commun. 345(1) Pages: 169-174
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Biodegradation of dioxins by recombinant Escherichia coli expressing rat CYP1A1 or its mutant. (2006)
  • Author(s): Shinkyo, R., Kamakura, M., Ikushiro, S.-I., Inouye, K., and Sakaki, T.
  • Journal Title: Appl. Microbiol. Biotechnol. 73(3) Pages: 584-590
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Uronic acid determination by high performance liquid chromatography with postcolumn fluorescent derivatization. (2006)
  • Author(s): Kakita, H., Kamishima, H., and Inouye, K.
  • Journal Title: J. Chromatogr. A1129(2) Pages: 296-299
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Improving the activity and stability of thermolysin by site-directed mutagenesis. (2006)
  • Author(s): Yasukawa, K. and Inouye, K.
  • Journal Title: Biochim. Biophys. Acta 1774(10) Pages: 1281-1288
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 微生物アミラーゼを用いるデンプン加工工業の問題点と今後の課題(総説). (2006)
  • Author(s): 長谷川信弘、井上國世
  • Journal Title: Bio Industry 10 Pages: 23-31
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] プロテアーゼによるダイズタンパク質の消化分解と特性の改変(総説). (2006)
  • Author(s): 井上國世
  • Journal Title: Bio Industry 10 Pages: 40-48
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Dissociation/association properties of a dodecameric cyclomaltodextrinase. Effects of pH and salt concentration on the oligomeric state (2006)
  • Author(s): Lee, H.-S., Kim, J.-S., Shim, K., Kim, J.-W., Inouye, K., Oneda, H., Kim, Y.-W., Cheong, K.-A., Cha, H., Woo, E.-J., Auh, J. H., Lee, S.-J., Kim, J.-W., Park, K.-H
  • Journal Title: FEBS J 273 Pages: 109-121
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Extracellular production of recombinant thermolysin expressed in Escherichia call and its purification and enzymatic characterization (2006)
  • Author(s): Inouye, K., Minoda, M., Takita, T., Sakurama, H., Hashida, Y., Kusano, M., Yasukawa, K
  • Journal Title: Protein Expr. Pm-if 46 Pages: 248-255
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin (2006)
  • Author(s): Yasukawa, K., Kusano, M., Nakamura, K., Inouye, K
  • Journal Title: Protein Expr. Purif 46 Pages: 332-336
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.) (2006)
  • Author(s): Nakazaki, T., Tsukiyama, T., Okumoto, Y, Kageyama, D., Naito, K., Inouye, K., Tanisaka, T
  • Journal Title: Genome 49(6) Pages: 619-630
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Purification and some properties of a D-fructose-1,6-bisphosphate aldolase from red alga, Gracilaria chorde Holmes (2006)
  • Author(s): Kakita, H., Kamishima, H., Inouye, K
  • Journal Title: Botanica Marina 49(2) Pages: 174-181
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure-function analysis of vitamin D 24-hydroxylase (CYP24A1) by site-directed mutagenesis : amino acid residues responsible for species-based difference of CYP24A1 between humans and rats (2006)
  • Author(s): Hamamoto, H., Kusudo, T., Urushino, N., Masuno, H., Yamamoto, K., Yamada, S., Kamakura, M., Ohta, M., Inouye, K., Sakaki, T
  • Journal Title: Mal. Pharmacol 70(1) Pages: 120-128
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interaction between mitochondrial CYP27B1 and adrenodoxin : Role of arginine 458 of mouse CYP27B1 (2006)
  • Author(s): Urushino, N., Yamamoto, K., Kagawa, N., Ikushiro, S., Kamakura, M., Yamada, S., Kato, S., Inouye, K., Sakaki, T
  • Journal Title: Biochemistry 45(14) Pages: 4405-4412
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Engineering of the pH-dependence of thermolysin activity as examined by site-directed mutagenesis of Asn112 located at the active site of thermolysin (2006)
  • Author(s): Kusano, M., Yasukawa, K., Hashida, Y., Inouye, K
  • Journal Title: J. Biochem 139(6) Pages: 1017-1023
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of starch hydrolysis activity and thermal stability of two Bacillus licheniformis a-amylases and insights into engineering a-amylase variants active at acidic conditions (2006)
  • Author(s): Lee, S., Oneda, H., Minoda, M., Tanaka, A., Inouye, K
  • Journal Title: J. Biochem 139(6) Pages: 997-1005
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparison of the wild-type a-amylase and its variant enzymes of Bacillus amyloliquefaciens in their activity and thermal stability, and insights into engineering the thermal stability of Bacillus a-amylase (2006)
  • Author(s): Lee, S., Mouri, Y., Minoda, M., Oneda, H., Inouye, K
  • Journal Title: J. Biochem 139(6) Pages: 1007-1015
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Efficient solubilization, activation, and purification of recombinant Cry45Aa of Bacillus thuringiensis expressed as inclusion bodies in Escherichia coli (2006)
  • Author(s): Okumura, S., Saitoh, H., Wasano, N., Katayaiha, H., Higuchi, K., Mizuki, E., Inouye, K
  • Journal Title: Protein Expr. Purl 47(1) Pages: 144-151
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of high concentration of salts on the esterase activity and structure of kiwifruit peptidase, actinidain (2006)
  • Author(s): Morimoto, K., Furuta, E., Hashimoto, H., Inouye, K
  • Journal Title: J. Biochem 139(6) Pages: 1065-1071
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enzymatic properties of human CYP2W1 expressed in Escherichia call (2006)
  • Author(s): Yoshioka, H., Kasai, N., Ikushiro, S., Shinkyo, R., Kamakura, M., Ohta, M., Inouye, K., Sakaki, T
  • Journal Title: Biochem. Biophys. Res. Commun 345(1) Pages: 169-174
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Biodegradation of dioxins by recombinant Escherichia coli expressing rat CYP1A1 or its mutant (2006)
  • Author(s): Shinkyo, R., Kamakura, M., Ikushiro, S. -I., Inouye, K., Sakaki, T
  • Journal Title: Appl. Microbial Biotechnoi 73(3) Pages: 584-590
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Uronic acid determination by high performance liquid chromatography with postcolumn fluorescentderivatization (2006)
  • Author(s): Kakita, H., Kamishima, H., Inouye, K
  • Journal Title: J. Chromatogr 1129(2) Pages: 296-299
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Problems and future perspectives in the starch processing industry using bacterial amylases (Review, in Japanese) (2006)
  • Author(s): Hasegawa, N., Inouye, K
  • Journal Title: Bio Industry 10 Pages: 23-31
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Digestion of soy-proteins by proteases and improvement of the chemical characteristics of the proteins (Review, in Japanese) (2006)
  • Author(s): Inouye, K
  • Journal Title: Bio Industry 10 Pages: 40-48
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Introduction to Enzyme and Coenzyme Chemistry (translation, in Japanese) (2006)
  • Author(s): Inouye, K
  • Journal Title: Springer-Verlag-Tokyo Pages: 320
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anomalous pH-dependence of the activity of human matrilysin(matrix metalloproteinase-7) as revealed by nitration and amination of its tyrosine residues. (2005)
  • Author(s): Muta, Y., Oneda, H., and Inouye, K.
  • Journal Title: Biochem. J. 386(2) Pages: 263-270
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Metabolism of 26, 26, 26, 27, 27, 27-F_6-1α, 23S, 25-trihydroxy vitamin D3 by human UDP-glucuronosyltransferase 1A3. (2005)
  • Author(s): Kasai, N., Sakaki, T., Shinkyo, R., Ikushiro, S. -i., Iyanagi, T., Kamao, M., Ohta, M., and Inouye, K.
  • Journal Title: Drug Metabol. Dispos. 33(1) Pages: 102-107
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Functional properties of soy protein hydrolysates obtained by selective proteolysis. (2005)
  • Author(s): Tsumura, K., Saito, T., Tsuge K., Ashida, H., Kugimiya, W., and Inouye, K.
  • Journal Title: Food Sci. Technol. /LWT 38(3) Pages: 255-261
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Metabolism of vitamin D3 by cytochromes P450. (2005)
  • Author(s): Sakaki, T., Kagawa, N., Yamamoto, K., and Inouye, K.
  • Journal Title: Front. Biosci. 10(1) Pages: 119-134
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Evidence of the occurrence of membrane-type serine protease 1/matriptase on the basolateral sides of enterocytes. (2005)
  • Author(s): Tsuzuki, S., Murai, N., Miyake, Y., Inouye, K., Iwanaga, T., and Fushiki, T.
  • Journal Title: Biochem. J. 388(2) Pages: 679-687
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Emulsifying properties of a peptide from peptic hydrolysates of soy glycinin. (2005)
  • Author(s): Tsumura, K., Kugimiya, W., and Inouye, K.
  • Journal Title: Food Sci. Technol. Res. 11(1) Pages: 46-51
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Preliminary X-ray crystallographic analysis of thermolysin in the presence of 4 M NaCl. (2005)
  • Author(s): Kamo, M., Inouye, K., Nagata, K., and Tanokura, M.
  • Journal Title: Acta Crystallogr. D61(6) Pages: 710-712
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Measurement and characterization of C-3 epimerization activity toward vitamin D3. (2005)
  • Author(s): Kamao, M., Hatakeyama, S., Sakaki, T., Sawada, N., Inouye, K., Kubodera, N., Reddy G. S., and Okano, T.
  • Journal Title: Arch. Biochem. Biophys. 436(1) Pages: 196-205
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of amino acid residues of CYP27B1 responsible for binding of 25-hydroxyvitamin D3 whose mutation causesvitamin D3-dependent rickets type I. (2005)
  • Author(s): Yamamoto, K., Uchida, E., Urushino, N. Sakaki, T., Kagawa, N., Sawada, N., Kamakura, M., Kato, S., Inouye, K., and Yamada, S.
  • Journal Title: J. Biol. Chem. 280(34) Pages: 30511-30516
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Catalytic properties of an organic solvent resistant tyrosinase from Streptomyces sp. REN-21 and. its high-level production in Escherichia coli. (2005)
  • Author(s): Ito, M. and Inouye, K.
  • Journal Title: J. Biochem. 138(4) Pages: 355-362
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Identification of a novel cytotoxic proteins, Cry45Aa, from Bacillus thuringiensis A1470 strain and its selective cytotoxic activity against various mammalian cell lines. (2005)
  • Author(s): Okumura, S., Saitoh, H., Ishikawa, T., Wasano, N., Yamashita, S., Kusumoto, K., Akao, T., Mizuki, E., Ohba, M., and Inouye, K.
  • Journal Title: J. Agric. Food Chem. 53(16) Pages: 6313-6318
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Metabolism of 2α-propexy-1α, 25-dihydroxyvitamin D3 by human CYP27A1 and CYP24A1. (2005)
  • Author(s): Abe, D., Sakaki, T., Kusudo, T., Kittaka, A., Saito, N., Suhara, Y., Fujishima, T., Takayama, H., Kamakura, M., Ohta, M., and Inouye, K.
  • Journal Title: Drug Metabol. Dispos. 33(6) Pages: 778-784
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mutational effect for stability in a conserved region of thermolysin. (2005)
  • Author(s): Matsumiya, Y., Nishikawa, K., Inouye, K., and Kubo, M.
  • Journal Title: Lett. Appl. Microbiol. 40 Pages: 329-334
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Rat cytochrome P450-mediated transformation of dichlorodibe nzo-p-dioxins by recombinant white-rot basidiomycete Coriolus hirsutus. (2005)
  • Author(s): Orihara, K., Yamazaki, T., Shinkyo, R., Sakaki, T., Inouye, K., Tsukamoto, A., Sugiura, J., and Shishido, K.
  • Journal Title: Appl. Microbiol. Biotechnol. 69 Pages: 22-28
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Determination of biotinylated proteins as an index for purification of plasma membrane using surface plasmon resonance-based optical biosensor. (2005)
  • Author(s): Okumura, S., Akao, T., Yamashita, S., Ichimatsu, T., and Inouye, K.
  • Journal Title: Cytotechnology 47 Pages: 59-67
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Synthesis of 25-hydroxy-19-norvitamin D3 analogs and their antiproliferative activities on prostate cells. (2005)
  • Author(s): Arai, M. A. M., Tsutsumi, R., Hara, H., Chen, T. C., Sakaki, T., Urushino, N., Inouye, K., and Kittaka, A.
  • Journal Title: Heterocycles 66(1) Pages: 469-479
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Anomalous pH-dependence of the activity of human matrilysin (matrix metalloproteinase-7) as revealed by nitration and amination of its tyrosine residues (2005)
  • Author(s): Muta, Y., Oneda, H., Inouye, K
  • Journal Title: Biochem. J 386(2) Pages: 263-270
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metabolism of 26, 26, 26, 27, 27, 27-Fs-la, 23S, 25-trihydroxyvitamin D3 by human UDP-glucuronosyltransferase 1A3 (2005)
  • Author(s): Kasai, N., Sakaki, T., Shinkyo, R., Ikushiro, Iyanagi, T., Kamao, M., Ohta, M., Inouye, K
  • Journal Title: Drug Metabol Dispos 33(1) Pages: 102-107
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional properties of soy protein hydrolysates obtained by selective proteolysis (2005)
  • Author(s): Tsumura, K., Saito, T., Tsuge, K., Ashida, H., Kugimiya, W., Inouye, K
  • Journal Title: Food Sci. Technol/LWT 38(3) Pages: 255-261
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metabolism of vitamin D3 by cytochromes P450 (2005)
  • Author(s): Sakaki, T., Kagawa, N., Yamamoto, K., Inouye, K
  • Journal Title: Front. Biosci 10(1) Pages: 119-134
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Evidence of the occurrence of membrane-type serine protease 1/matriptase on the basolateral sides of enterocytes (2005)
  • Author(s): Tsuzuki, S., Murai, N., Miyake, Y., Inouye, K., Iwanaga, T., Fushiki, T
  • Journal Title: Biochem. J 388(2) Pages: 679-687
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Emulsifying properties of a peptide from peptic hydrolysates of soy glycinin (2005)
  • Author(s): Tsumura, K., Kugimiya, W., Inouye, K
  • Journal Title: Food Sci. Technol. Res 11(1) Pages: 46-51
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Preliminary X-ray crystallographic analysis of thermolysin in the presence of 4 M NaCI (2005)
  • Author(s): Kamo, M., Inouye, K., Nagata, K., Tanokura, M
  • Journal Title: Acta Crystallogr 61(6) Pages: 710-712
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Measurement and characterization of C-3 epimerization activity toward vitamin D3 (2005)
  • Author(s): Kamao, M., Hatakeyama, S., Sakaki, T., Sawada, N., Inouye, K., Kubodera, N., Reddy, G., S., Okano, T
  • Journal Title: Arch. Biochem. Biophys 436(1) Pages: 196-205
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of amino acid residues of CYP27B1 responsible for binding of 25-hydroxyvitamin D3 whose mutation causes vitamin D3-dependent rickets type I (2005)
  • Author(s): Yamamoto, K., Uchida, E., Urushino, N. Sakaki, T., Kagawa, N., Sawada, N., Kamakura, M., Kato, S., Inouye, K., Yamada, S
  • Journal Title: J. Biol. Chem 280(34) Pages: 30511-30516
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Catalytic properties of an organic solvent resistant tyrosinase from Streptomyces sp. REN-21 and its high-level production in Escherichia colt (2005)
  • Author(s): Ito, M., Inouye, K
  • Journal Title: J. Biochem 138(4) Pages: 355-362
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a novel cytotoxic proteins, Cry45Aa, from Bacillus thuringiensis A1470 strain and its selective cytotoxic activity against various mammalian cell lines (2005)
  • Author(s): Okumura, S., Saitoh, H., Ishikawa, T., Wasano, N., Yamashita, S., Kusumoto, K., Akao, T., Mizuki, E., Ohba, M., Inouye, K
  • Journal Title: J. Agric, Food Chem 53(16) Pages: 6313-6318
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Metabolism of 2a-propexy-la, 25-dihydroxyvitamin D3 by human CYP27A1 and CYP24A1 (2005)
  • Author(s): Abe, D., Sakaki, T., Kusudo, T., Kittaka, A., Saito, N., Suhara, Y., Fujishima, T., Takayama, H., Kamakura, M., Ohta, M., Inouye, K
  • Journal Title: Drug Metabol. Dispos 33(6) Pages: 778-784
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mutational effect for stability in a conserved region of thermolysin (2005)
  • Author(s): Matsumiya, Y., Nishikawa, K., Inouye, K., Kubo, M
  • Journal Title: Lett Aug Mthrobiol 40 Pages: 329-334
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Rat cytochrome P450-mediated transformation of dichlorodibenzo-p-dioxins by recombinant white-rot basidiomycete Coriolus hirsutus (2005)
  • Author(s): Orihara, K., Yamazaki, T., Shinkyo, R., Sakaki, T., Inouye, K., Tsukamoto, A., Sugiura, J., Shishido, K
  • Journal Title: Appl. Microbial. Bioteclmol 69 Pages: 22-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Determination of biotinylated proteins as an index for purification of plasma membrane using surface plasmon resonance-based optical biosensor (2005)
  • Author(s): Okumura, S., Akao, T., Yamashita, S., Ichimatsu, T., Inouye, K
  • Journal Title: Cytotechnology 47 Pages: 59-67
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Synthesis of 25-hydroxy-19-norvitamin D3 analogs and their antiproliferative activities on prostate cells (2005)
  • Author(s): Arai, M., A., M., Tsutsumi, R., Hara, H., Chen, T., C., Sakaki, T., Urushino, N., Inouye, K., Kittaka, A
  • Journal Title: Heterocycles 66(1) Pages: 469-479
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional glyco-materials : Their development and application to foods (2005)
  • Author(s): Inouye, K
  • Journal Title: CMC Pages: 447
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functions of carbohydrates (2005)
  • Author(s): Inouye, K
  • Journal Title: Functional glyco-materials : Their development and application to foods (Ed. by Inouye, K.) 3-23 Pages: 447
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Production of glycosylated ascorbic acid and antioxidative function for foods.1 (2005)
  • Author(s): Inouye, K
  • Journal Title: Functional glyco-materials : Their development and application to foods (Ed. by Inouye, K.) 284-294 Pages: 447
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Enzyme chemical properties of Streptomyces griseolus CYP1051A1 (2008)
  • Author(s): Shinkyo, R., Kamakura, M., Ikushiro, S., Inouye, K., Sakaki, T
  • Organizer: 2005 Annual Meeting of the Japan Streptomyces Society
  • Place of Presentation: Wing Takaoka, Toyama
  • Year and Date: 2008-09-08
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 4,5-dicaffeoylquinic acidによるブタすい臓α-アミラーゼ阻害作用の解析. (2008)
  • Author(s): 成田優作, 木村良太郎, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 順相分配HPLCを用いたウロン酸類の分離. (2008)
  • Author(s): 垣田浩孝, 上嶋洋, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヒトマトリックスメタロプロテイナーゼ7(MMP-7)の活性解離の推定. (2008)
  • Author(s): 竹治仁詩, 保川清, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヒトマトリックスメタロプロテイナーゼ7(MMP-7)との相互作用によるカテキンの蛍光特性の変化. (2008)
  • Author(s): 三宅智子, 保川清, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] シマミミズ由来の植物ウイルス感染阻害能を有するプロテアーゼについて. (2008)
  • Author(s): 上田光宏, 山下崇之, 野田佳奈子, 中澤昌美, 宮武和孝, 大木理, 坂口実, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 蛍光分光法を用いるサーモライシン(TLN)の塩による活性化機構の解析. (2008)
  • Author(s): 加茂昌之、井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Bacillus amyloliquefaciens中性金属プロテイナーゼの活性に対する温度,pH, Naclの効果ならびにサーモライシンとの比較. (2008)
  • Author(s): 福井善裕, 橋田泰彦, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サーモライシン(TLN)の活性部位への変異導入による活性への効果 (2008)
  • Author(s): 草野正雪, 保川清, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 成熟型配列とプロ配列の共発現による酵素活性及び熱安定性が向上した変異型サーモライシン(TLN)の大腸菌からの菌体外生産. (2008)
  • Author(s): 保川清, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 糖類の添加によるサーモライシンの熱安定性と溶解度の向上. (2008)
  • Author(s): 橋田泰彦, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] AMV逆転写酵素とMMLV逆転写酵素の熱安定性の比較. (2008)
  • Author(s): 根本大資, 保川清, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Bacillus stearothermophilus由来リジル-tRNA合成酵素の触媒ドメインとアンチコドン結合ドメイン間の相互作用による気質認識の変化に関するTrp蛍光解析. (2008)
  • Author(s): 猿渡由寛, 滝田禎亮, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 大豆タンパク質のサーモライシン(TLN)による加水分解に伴う凝集反応の解析. (2008)
  • Author(s): 浅岡浩平, 保川清, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibitory actiovity of 4,5-dicaffeoylquinic acid against porcine pancreas a-amylase (2008)
  • Author(s): Narita, Y., Kimura, R., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Mello University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Separation of uronic acids using normal-phase partition HPLC (2008)
  • Author(s): Kakita, H., Kamishima, H., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Mello University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Determination of the catalytic residues of human matrix metalloproteinase-7 (MMP-7) (2008)
  • Author(s): Takeharu, H., Yasukawa, K., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Mello University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fluorescence change of catechins on the interaction with human matrix metalloproteinase-7 (MMP-7) (2008)
  • Author(s): Miyake, T., Yasukawa, K., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Earthworm proteases inhibiting plant virus infection (2008)
  • Author(s): Ueda, M., Yamashita, T., Noda, K., Nakazawa, M., Miyatake, K., Ohki, S., Sakaguchi, M., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Comparison of the effects of temperature, pH, and NaC1 on Bacillus amyloliquefaciens neutral metalloproteinase activity with those on thermolysin activity (2008)
  • Author(s): Fukui, Y., Hashida, Y., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of site-directed mutagenesis of thermolysin (2008)
  • Author(s): Kusano, M., Yasukawa, K., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Extracellular production of thermolysin variants with higher activity and stability by coexpression of the mature sequence and pro-sequence in E. coli. (2008)
  • Author(s): Yasukawa, K., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meija University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] improvement of stability and solubility of thermolysin by the addition of sugars (2008)
  • Author(s): Hashida, Y., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Comparison between AMV and MMLV reverse transcriptases with respect to the thermal stability (2008)
  • Author(s): Nemoto, D., Yasukawa, K., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Interaction between the catalytic domain and anti-codon domain of Bacillus stearothermophilus lysyl-tRNA synthetase as examined by fluorescence (2008)
  • Author(s): Saruwatari, Y., Takita, T., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo, University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Kinetics on the aggreagation reaction of soyproteins accompanied by the hydrolysis by thermolysin (2008)
  • Author(s): Asaoka, K., Yasukawa, K., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 緑茶カテキン類のブタすい臓由来α-アミラーゼに対する阻害効果. (2008)
  • Author(s): 河瀬美紀, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 小麦由来α-アミラーゼ阻害物質0.19AIの阻害活性に対するニトロ化とアミノ化の効果. (2008)
  • Author(s): 河瀬美紀, 井上國世
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イネクラスIIキチナーゼCht11の機能解析. (2008)
  • Author(s): 築山拓司, Xayphakatsa, Kosonh, 井上國世, 奥本裕, 中崎哲也, 谷坂隆俊
  • Organizer: 2008年度日本農芸化学会大会
  • Place of Presentation: 名城大学天白キャンパス
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibitory activity of green tea catechins against porcine pancreas a-amylase (2008)
  • Author(s): Kawase, M., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-lcai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of nitration and amination on the inhbitoiry activity of wheat a-amylase inhibitor 0.19 Al (2008)
  • Author(s): Kawase, M., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional analysis of rice class II chitinase Chtl1 (2008)
  • Author(s): Tsukiyama, T., Kosonh, X., Inouye, K., Okumoto, H., Nakazaki, T., Tanisaka, T
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fluorescence study on the activation mechanism of thermolysin induced by salts (2008)
  • Author(s): Kamo, M., Inouye, K
  • Organizer: 2008 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Meijo University, Nagoya
  • Year and Date: 2008-03-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Gene expression analysis and characterization of rice(Oryzasativa L.)class II chitinase CHT11. (2008)
  • Author(s): Tsukiyama, T., Inouye, K., Xayphakatsa, K., Okumoto, Y., Nakazaki, T., and Tanisaka, T.
  • Organizer: International Symposium on Frontier in Plant Proteome Research
  • Place of Presentation: Tsukuba Norin Hall, Tsukuba, Japan
  • Year and Date: 2008-03-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Gene expression analysis and characterization of rice (Oryza satia L.) class II chitinase CHT11 (2008)
  • Author(s): Tsukiyama, T., Inouye, K., Xayphakatsa, K., Okumoto, Y., Nakazaki, T., Tanisaka, T
  • Organizer: International Symposium on Frontier in Plant Proteome Research
  • Place of Presentation: Tsukuba Norin Hall, Tsukuba, Japanukuba Norin Hall, Tsukuba, Japan
  • Year and Date: 2008-03-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 酵素で病気を測る-より高感度により迅速に- (2008)
  • Author(s): 井上國世
  • Organizer: 第5回日本大学大学院生産工学研究科生命工学リサーチ・センター研究発表講演会
  • Place of Presentation: 日本大学生産工学部津田沼校舎
  • Year and Date: 2008-02-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Improvement of enzyme immunoassay in the sensitivity and rapidity (2008)
  • Author(s): Inouye, K
  • Organizer: 5th Conference of the Biotechnology Research Center of Nippon University
  • Place of Presentation: Nippon University, Narashino, Chiba
  • Year and Date: 2008-02-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of pH and NaCl on the inhibition of porcine pancreas alpha-amylase(PPA)by wheat kernel 0.19 alpha-amylase inhibitor(0.19 AI). (2007)
  • Author(s): Kawase, M., Lee, S., and Inouye, K.
  • Organizer: 2007 Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli. (2007)
  • Author(s): Yasukawa, K., Kusano, M., and Inouye, K.
  • Organizer: 2007 Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Improving the activity and stability of thermolysin by site-directed mutagenesis. (2007)
  • Author(s): Yasukawa, K. and Inouye, K.
  • Organizer: 2007 Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of pH and NaC1 on the inhibition of porcine pancreas alpha-amylase (PPA) by wheat kernel 0.19 alpha-amylase inhibitor (0.19 Al) (2007)
  • Author(s): Kawase, M., Lee, S., Inouye, K
  • Organizer: 2007 Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli (2007)
  • Author(s): Yasukawa, K., Kusano, M., Inouye, K
  • Organizer: 2007 Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Improving the activity and stability of thermolysin by site-directed mutagenesis (2007)
  • Author(s): Yasukawa, K., Inouye, K
  • Organizer: 2007 Annual Meeting of the Molecular Biology Society of Japan and the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 2007-12-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibition of human matrix metalloproteinase 7(MMP-7)by green tea cathechins and lignans and insights into their structure-activity relationship. (2007)
  • Author(s): Inouye, K., Muta, Y., Oyama, S., Oneda, H., Umezawa, T., and Shimada, M.
  • Organizer: 3rd International Conference on Polyphenols and Health(ICPH2007)
  • Place of Presentation: Kyoto International Conference Hall, Kyoto, Janan
  • Year and Date: 2007-11-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibition of human matrix metalloproteinase 7 (MMP-7) by green tea cathechins and lignans and insights into their structure-activity relationship (2007)
  • Author(s): Inouye, K., Muta, Y., Oyama, S., Oneda, H., Umezawa, T., Shimada, M
  • Organizer: 3rd International Conference on Polyphenols and Health (ICPH2007)
  • Place of Presentation: Kyoto International Conference Hall, Kyoto, Japan
  • Year and Date: 2007-11-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Role of Tyr-219 in the enormously wide pH-dependence of human matrix metalloproteinase 7(MMP-7)activity. (2007)
  • Author(s): Inouye, K., Muta, Y., Yasui, N., Matsumiya, M., and Kubo, M.
  • Organizer: 5th General Meeting of the International Proteolysis Society
  • Place of Presentation: University of Patras, Patras, Greece
  • Year and Date: 2007-10-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Role of Tyr-219 in the enormously wide pH-dependence of human matrix metalloproteinase 7 (MMP-7) activity (2007)
  • Author(s): Inouye, K., Muta, Y., Yasui, N., Matsumiya, M., Kubo, M
  • Organizer: 5th General Meeting of the International Proteolysis Society
  • Place of Presentation: University of Patras, Patras, Greece
  • Year and Date: 2007-10-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 成熟型配列とプロ配列の共発現による組み換えサーモライシンの大腸菌からの菌体外生産. (2007)
  • Author(s): 保川清, 草野正雪, 井上國世
  • Organizer: 第59回日本生物工学会大会
  • Place of Presentation: 広島大学総合科学部東広島キャンパス
  • Year and Date: 2007-09-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Extracellular production of recombinant thermolysin by co-expression of mature sequence and pro-sequence (2007)
  • Author(s): Yasukawa, K., Kusano, M., Inouye, K
  • Organizer: 59th Japanese Society of Bioengineering
  • Place of Presentation: School of Integrated Sciences, Hiroshima University, Higashi Hiroshima
  • Year and Date: 2007-09-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Bacillus amyloliquefaciens中性金属プロテアーゼ(BaNP)の酵素化学的性質の解析. (2007)
  • Author(s): 福井善裕, 橋田泰彦, 井上國世
  • Organizer: 日本農芸化学会2007年度関西中部支部合同大会
  • Place of Presentation: 中部大学春白井キャンパス
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サーモライシンの活性部位への変異導入による活性への効果. (2007)
  • Author(s): 草野正雪, 保川清, 井上國世
  • Organizer: 日本農芸化学会2007年度関西中部支部合同大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] カテキンとヒトマトリックスメタロプロテイナーゼ7(MMP-7)の相互作用の蛍光測定による解析. (2007)
  • Author(s): 三宅智子, 保川清, 井上國世
  • Organizer: 日本農芸化学会2007年度関西中部支部合同大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヒトマトリックスメタロプロテイナーゼ7(MMP-7)の活性解離基の推定. (2007)
  • Author(s): 竹治仁詩, 保川清, 井上國世
  • Organizer: 日本農芸化学会2007年度関西中部支部合同大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] グルテンのプロテアーゼ加水分解による溶解度の上昇. (2007)
  • Author(s): 富永良太, 保川清, 井上國世
  • Organizer: 日本農芸化学会2007年度関西中部支部合同大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] AMV逆転写酵素とMMLV逆転写酵素の熱安定性の比較. (2007)
  • Author(s): 根本大資, 保川清, 井上國世
  • Organizer: 日本農芸化学会2007年度関西中部支部合同大会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Enzyme chemical properties of Bacillus amyloliquefaciens neutral metalloproteinase (BaNP) (2007)
  • Author(s): Fukui, Y., Hashida, Y., Inouye, K
  • Organizer: 2007 Joint Meeting of Kansai-branch and Chubu-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Chubu University. Kasugai, Aichi
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of site-directed mutagenesis of thermolysin on its catalytic activity (2007)
  • Author(s): Kusano, M., Yasukawa, K., Inouye, K
  • Organizer: 2007 Joint Meeting of Kansai-branch and Chubu-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Chubu University, Kasugai, Aichi
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Fluorophotometric analysis of the interaction between catechins and human matrix metalloproteinase-7 (MMP-7) (2007)
  • Author(s): Miyake, T., Yasukawa, K., Inouye, K
  • Organizer: 2007 Joint Meeting of Kansai-branch and Chubu-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Chubu University, Kasugai, Aichi
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Evaluation of catalytic residues of human matrix metalloproteinase-7 (MMP-7) (2007)
  • Author(s): Takeharu, H., Yasukawa, K., Inouye, K
  • Organizer: 2007 Joint Meeting of Kansai-branch and Chubu-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Chubu University, Kasugai, Aichi
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Increase in solubility of thermolysin by treatment of proteases (2007)
  • Author(s): Tominaga, R., Yasukawa, Yasukawa, K., Inouye, K
  • Organizer: 2007 Joint Meeting of Kansai-branch and Chubu-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Chubu University, Kasugai, Aichi
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Thermal stability of AMV and MMLV reverse transcriptases (2007)
  • Author(s): Nemoto, D., Yasukawa, K., Inouye, K
  • Organizer: 2007 Joint Meeting of Kansai-branch and Chubu-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Chubu University, Kasugai, Aichi
  • Year and Date: 2007-09-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 金属プロテアーゼの活性解離基と反応機構. (2007)
  • Author(s): 井上國世
  • Organizer: 酵素化学ミニシンポジウム「酵素化学において過去10年間に解明されたこと、今後10年間に解明すべきこと」
  • Place of Presentation: 京大会館
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The catalytic residues and catalytic mechanism of metalloproteinases (2007)
  • Author(s): Inouye, K
  • Organizer: Enzyme Chemistry Symposiu
  • Place of Presentation: Kyodai Kaikan, Kyoto
  • Year and Date: 2007-09-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 部位特異的変異によるサーモライシンの改変. (2007)
  • Author(s): 保川清, 草野正雪, 辰巳智香, 橋田泰彦, 井上國世
  • Organizer: 食品酵素化学研究会第7回学術講演会
  • Place of Presentation: 京大会館
  • Year and Date: 2007-09-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Improvement of the activity and stability of thermolysin by site-directed mutagenesis (2007)
  • Author(s): Yasukawa, K., Kusano, M., Tatsumi, C., Hashida, Y., Inouye, K
  • Organizer: 7th Conference of Food Enzyme Chemistry
  • Place of Presentation: Kyodai Kaikan, Kyoto
  • Year and Date: 2007-09-09
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular engineering and regulation of the activity of Bacillus a-amylases for the application to food science and biotechnology (2007)
  • Author(s): Inouye, K
  • Organizer: JSPS-KOSEF Core University Program Joint Seminar
  • Place of Presentation: Kyoto University Uji Campus, Uji, Kyoto
  • Year and Date: 2007-07-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular engineering and regulation of the activity of Bacillus α-amylases for the application to food science and biotech nology. (2007)
  • Author(s): Inouye, K.
  • Organizer: JSPS-KOSEF Core University Program Joint Seminar
  • Place of Presentation: Kyoto University Uji Campus
  • Year and Date: 2007-06-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Reaction mechanism inhibitory effects of green tea catechins and lignans on MMP-7 activity. (2007)
  • Author(s): Inouye, K.
  • Organizer: UK-Japan Mini-symposium on the Recent Progress of Enzyme Inhibition Mechanism
  • Place of Presentation: University of Warwick, Department of Chemistry
  • Year and Date: 2007-06-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Reaction mechanism inhibitory effects of green tea catechins and lignans on MMP-7 activity (2007)
  • Author(s): Inouye, K
  • Organizer: UK-Japan Mini-symposium on the Recent Progress of Enzyme Inhibition Mechanism
  • Place of Presentation: Department of Chemistry, University of Warwick
  • Year and Date: 2007-06-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] コムギ由来α-アミラーゼ阻害剤0.19AIによるブタすい臓α-アミラーゼの阻害に及ぼすpHとNaClの効果. (2007)
  • Author(s): 河瀬美紀, 李承在, 井上國世
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 京都工芸繊維大学松ケ崎キャンパス
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラットMembrane-type serine protease1のラットhepatocyte growth factor activator inhibitor type-1による阻害機構の解析. (2007)
  • Author(s): 兒島憲二, 都築巧, 伏木亨, 井上國世
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 京都工芸繊維大学松ケ崎キャンパス
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 部位特異的委異によるサーモライシンの改変. (2007)
  • Author(s): 保川清, 草野正雪, 辰巳智香, 橋田泰彦, 井上國世
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 京都工芸繊維大学松ケ崎キャンパス
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] トレハロースによるサーモライシンの熱安定性の向上とその熱力学的解析. (2007)
  • Author(s): 橋田泰彦, 井上國世
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 京都工芸繊維大学松ケ崎キャンパス
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] リジル-tRNA 合成酵素におけるドメイン間相互作用に起因する構造変化のTrp蛍光解析. (2007)
  • Author(s): 猿渡由寛, 滝田禎亮, 井上國世
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 京都工芸繊維大学松ケ崎キャンパス
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Site-directed mutagenesis of thermolysin (2007)
  • Author(s): Yasukawa, K., Kusano, M., Tatsumi, C., Hashida, Y., Inouye, K
  • Organizer: 57th Kinki-branch Meeting of the Biochemical Society of Japan
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Thermodynamic analysis of enhancement of thermal stability of thermolysin induced by trehalose (2007)
  • Author(s): Hashida, Y., Inouye, K
  • Organizer: 57th Kinki-branch Meeting of the Biochemical Society of Japan
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Change in Trp-fluorescence associated with the conformational change of Bacillus sterothermophilus lysyl-tRNA synthetase (2007)
  • Author(s): Saruwatari, Y., Takita, T., Inouye, K
  • Organizer: 57th Kinki-branch Meeting of the Biochemical Society of Japan
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2007-05-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibitory effects of green tea catechins and lignans on MMP-7 activity. (2007)
  • Author(s): Inouye, K.
  • Organizer: International Symposium on the Clinical Aspects of Matrix Metalloproteinases
  • Place of Presentation: University of Leiden Medical Center
  • Year and Date: 2007-04-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibitory effects of green tea catechins and lignans on MMP-7 activity (2007)
  • Author(s): Inouye, K
  • Organizer: International Symposium on the Clinical Aspects of Matrix Metalloproteinases
  • Place of Presentation: University of Leiden Medical Center
  • Year and Date: 2007-04-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Bacillus sterothermophilus由来リジル-tRNA合成酵素の構造変化に関するTrp蛍光解析. (2007)
  • Author(s): 猿渡由寛, 滝田禎亮, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] クロロゲン酸によるブタ膵臓α-アミラーゼ阻害作用の解析. (2007)
  • Author(s): 成田優作, 李承在, 木村良太郎, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ウロン酸分離への順相分配クロマトグラフィーの応用. (2007)
  • Author(s): 垣田浩孝, 上嶋洋, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Change in Trp-fluorescence associated with the conformational change of Bacillus sterothermophilus lysyl-tRNA synthetase (2007)
  • Author(s): Saruwatari, Y., Takita, T., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Kinetic analysis of the inhibition of porcine pancreas a-amylase by chrologenic acids (2007)
  • Author(s): Narita, Y., Lee, S., Kimura, R., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Application of norml-phase chromatography to the separation of uronic acids (2007)
  • Author(s): Kakita, H., Kamishima, H., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 小麦由来α-アミラーゼ阻害剤0.19AIによるブタすい臓α-アミラーゼの阻害に対するpHの効果. (2007)
  • Author(s): 河瀬美紀, 李承在, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Membrane-type serine protease1(MT-SP1)の阻害におけるhepatocyte growth factor activator inhibitor type-1(HAI-1)の各ドメインの役割. (2007)
  • Author(s): 兒島憲二, 都築巧, 伏木亨, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Gly-D-Pheをサーモライシン(TLN)のリガンドとしたアフィニティーゲルの性能に及ぼすスペーサーの長さの影響. (2007)
  • Author(s): 井上國世, 草野正雪, 保川清, 中村孝司
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 成熟型配列とプロ配列の共発現による組み換えサーモライシンの大腸菌からの菌体外生産. (2007)
  • Author(s): 草野正雪, 保川清, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 蛍光法を用いるサーモライシン(TLN)の塩による活性化機構の解析. (2007)
  • Author(s): 加茂昌之, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] トレハロースおよび関連するその他の二糖類によるサーモライシンの熱安定性向上. (2007)
  • Author(s): 橋田泰彦, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 部位特異的変異によるサーモライシンの酵素活性および熱安定性の向上. (2007)
  • Author(s): 保川清, 岡本直子, 井上國世
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 変異型サーモライシンの自己分解点の改変による安定性向上の解析. (2007)
  • Author(s): 松宮芳樹, 村田尚子, 井上國世, 久保幹
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of pH on the inhibitory activity of wheat-amylase inhibitor 0.19AI against porcine pancreas-amylase (2007)
  • Author(s): Kawase, M., Lee, S., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Role of the domains of HAI-1 in the inhibition against membrane-type serine protease1 (MT-SP1) (2007)
  • Author(s): Kojima, K., Tsuzuki, T., Fushiki, T., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of the length of spacer in the affinity performance of the Gly-D-Phe ligand to thermolysin (2007)
  • Author(s): Inouye, K., Kusano, M., Yasukawa, K., Nakamura, T
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Extracellular production of recombinant thermolysin by coexpression of mature sequence and pro-sequence (2007)
  • Author(s): Kusano, M., Yasukawa, K., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Activation mechanism of thermolysin as examined by fluorescence spectrometry (2007)
  • Author(s): Kamo, M., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural UniSrersity
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Enhancement of thermal stability of thermolysin by trehalose and related disaccharides (2007)
  • Author(s): Hashida, Y., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Improvement of activity and stability of thermolysin by site-directed mutagenesis (2007)
  • Author(s): Yasukawa, K., Okamoto, N., Inouye, K
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Improvement of thermal stability of thermolysin by site-directed mutagenesis at the peptide bond most susceptible to autolysis (2007)
  • Author(s): Matsumiya, Y., Murata, H., Inouye, K., Kubo, M
  • Organizer: 2007 Annual Meeting of the Nippon Nougeikagaku-kai
  • Place of Presentation: Tokyo Agrucultural University
  • Year and Date: 2007-03-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of pH and NaCl on the inhibitory activity of wheat a-amylase inhibitor 0.19 AI against porcine pancreas a-amylase (2007)
  • Author(s): Kawase, M., Lee, S., Inouye, K
  • Organizer: 57th Kinki-branch Meeting of the. Biochemical Society of Japan
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2007-03-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibition mechanism of rat hepatocyte growth factor activator inhibitor type-lagainst rat membrane-type serine proteasel (2007)
  • Author(s): Kojima, K., Tsuzuki, T., Fushiki, T., Inouye, K
  • Organizer: 57th Kinki-branch Meeting of the Biochemical Society of Japan
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2007-03-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] キウイフルーツ果実に含まれる抗カビ蛋白質アイソフォームの精製. (2007)
  • Author(s): 森本康一, 國井沙織, 池上春香, 森山弘之, 井上國世
  • Organizer: 第2回産業用酵素シンポジウム
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2007-03-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Purification of isoforms of anti-fungal protein in kiwi fruit (2007)
  • Author(s): Morimoto, K., Kunii ; S., Ikegami, H., Moriyama, H., Inouye, K
  • Organizer: 2nd Symposium of Industrial Enzymes
  • Place of Presentation: Osaka Prefectural University
  • Year and Date: 2007-03-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] リグナン類を代表とする木質成分による哺乳類とくにヒトの酵素の機能調節に関する分子科学的解析. (2007)
  • Author(s): 井上國世
  • Organizer: 第65回生存圏シンポジウム:生存圏萌芽・融合ミッションシンポジウム
  • Place of Presentation: 京都大学生存圏研究所木質ホール(宇治キャンパス)
  • Year and Date: 2007-03-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecula Engineering and regulation of the activity of Bacillus α-amylase for their application to food science and biotechnology. (2007)
  • Author(s): Inouye, K.
  • Organizer: The Japan-Korea Food Enzyme Chemistry Symposium
  • Place of Presentation: Seoul National University College of Life Science and Agriculture
  • Year and Date: 2007-02-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular Engineering and regulation of the activity of Bacillus-amylase for their application to food science and biotechnology (2007)
  • Author(s): Inouye, K
  • Organizer: The Japan-Korea Food Enzyme Chemistry Symposium
  • Place of Presentation: College of Life Science and Agriculture, Seoul National University
  • Year and Date: 2007-02-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 酵素を用いて病気を測る-より高感度に、より迅速に-. (2006)
  • Author(s): 井上國世
  • Organizer: 第5回京都大学農学研究科シンポジウム
  • Place of Presentation: 京都大学農学研究科総合館
  • Year and Date: 2006-11-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Measurent of diseases using enzymes, more sensitively and more rapidly (2006)
  • Author(s): Inouye, K
  • Organizer: 5th Symposium of Kyoto University Graduate School of Agriculture
  • Place of Presentation: Kyoto University Graduate School of Agriculture, Kyoto
  • Year and Date: 2006-11-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Purification and characterization of D-fructose-1, 6-bisphosphate aldolase from the red alga, Gracilaria chorda Holmes, collected from the Seto Inland Sea. (2006)
  • Author(s): Inouye, K., Kakita, H., and Kamishima, H.
  • Organizer: Joint Workshop for Research Cooperation Seafood Safety between Japan and Norway
  • Place of Presentation: Yokohama Japan
  • Year and Date: 2006-11-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Purification and characterization of D-fructose-1,6-bisphosphate aldolase from the red alga, Gracilaria chorda Holmes, collected from the Seto Inland Sea (2006)
  • Author(s): Inouye, K., Kakita, H., Kamishima, H
  • Organizer: Joint Workshop for Research Cooperation Seafood Safety between Japan and Norway
  • Place of Presentation: Yokohama Japan
  • Year and Date: 2006-11-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular engineering and regulation of the activity and stability of Bacillus α-amylase for their application to food science and biotechnology. (2006)
  • Author(s): Inouye, K., Lee, S., Mouri, S., Oneda, H., Tanaka, A., and Minoda, M.
  • Organizer: The Ninth Japan-China-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Piazza Omi, Ohtsu, Japan
  • Year and Date: 2006-11-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular engineering and regulation of the activity and stability of Bacillus a-amylase for their application to food science and biotechnology (2006)
  • Author(s): Inouye, K., Lee, S., Mouri, S., Oneda, H., Tanaka, A., Minoda, M
  • Organizer: The Ninth Japan-China-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Piazza Omi, Ohtsu, Japan
  • Year and Date: 2006-11-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] カテキンおよびリグナンのヒトマトリックスメタロプロテイナーゼ7(MMP-7)に対する阻害作用-構造機能相関およびガン転移抑制に対する期待-. (2006)
  • Author(s): 井上國世
  • Organizer: 生存圏研究所学際萌芽研究センター第38回定例オープンセミナー
  • Place of Presentation: 京都大学生存圏研究所(宇治キャンパス)
  • Year and Date: 2006-10-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibitory activity of catechins and lignans against human matrix metalloproteinase-7 (MMP-7) (2006)
  • Author(s): Inouye, K
  • Organizer: 38th Open Seminar of Kyoto University Biosphere Research Institute
  • Place of Presentation: Kyoto University Biosphere Research Institute, Uji, Kyoto
  • Year and Date: 2006-10-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ヒトマトリックスメタロプロテイナーゼ-7(MMP7)に対するヘパリンの効果. (2006)
  • Author(s): 保川清, 肥後仁大, 井上國世
  • Organizer: 日本農芸化学会2006年度関西支部会
  • Place of Presentation: 京都工芸繊維大学
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サーモライシンの熱安定性に対するトレハロースおよび関連するその他の二糖類の添加効果. (2006)
  • Author(s): 橋田泰彦, 井上國世
  • Organizer: 日本農芸化学会2006年度関西支部会
  • Place of Presentation: 京都工芸繊維大学
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Bacillus stearothermophilus由来リジルtRNA合成酵素:リジン及び中間体アナログ複合体のX線結晶構造解析. (2006)
  • Author(s): 櫻間晴子, 滝田禎亮, 三上文三, 伊藤貴文, 井上國世
  • Organizer: 日本農芸化学会2006年度関西支部会
  • Place of Presentation: 京都工芸繊維大学
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ダイズ蛋白質の熱処理とプロテアーゼ加水分解に伴う凝集反応との関係. (2006)
  • Author(s): 浅岡浩平, 中野幹生, 保川清, 井上國世
  • Organizer: 日本農芸化学会2006年度関西支部会
  • Place of Presentation: 京都工芸繊維大学
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 成熟型配列とプロ配列の共発現による組み換えサーモライシンの大腸菌からの菌体外生産. (2006)
  • Author(s): 草野正雪, 保川清, 井上國世
  • Organizer: 日本農芸化学会2006年度関西支部会
  • Place of Presentation: 京都工芸繊維大学
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of heparin on matrix metalloproteinase-7 (MMP7) (2006)
  • Author(s): Ysukawa, K., Higo, H., Inouye, K
  • Organizer: 2006 Annual Meeting of the Kansai-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of trehalose and related disaccharides on the thermal stability of thermolysin (2006)
  • Author(s): Hashida, Y., Inouye, K
  • Organizer: 2006 Annual Meeting of the Kansai-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] X-ray crystallographic analysis of the Bacillus stearothermophilus lysyl-tRNA synthetase complexes with lysine and lysyl-adenylate analog (2006)
  • Author(s): Sakurama, H., Takita, T., Mikami, B., Ito, T., Inouye, K
  • Organizer: 2006 Annual Meeting of the Kansai-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Relationship between heat treatment of soyproteins and aggregation of the soyprotein fragments produced by proteolysis (2006)
  • Author(s): Asaoka, K., Nakano, M., Yasukawa, K., Inouye, K
  • Organizer: 2006 Annual Meeting of the Kansai-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Extracellular production of recombinant thermolysin by coexpression of mature sequence and nro-seauence (2006)
  • Author(s): Kusano, M., Yasukawa, K., Inouye, K
  • Organizer: 2006 Annual Meeting of the Kansai-branch of the Nippon Nougeikagaku-kai
  • Place of Presentation: Kyoto Institute of Technology
  • Year and Date: 2006-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] サーモライシンの活性部位のAsn112の変異による活性のpH依存性の改変. (2006)
  • Author(s): 草野正雪, 保川清, 橋田泰彦, 井上國世
  • Organizer: 第58回日本生物工学会大会
  • Place of Presentation: 阪大豊中キャンパス
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 変異サーモライシンの自己分解点の改変による安定性の向上. (2006)
  • Author(s): 村田尚子, 松宮芳樹, 井上國世, 久保幹
  • Organizer: 第58回日本生物工学会大会
  • Place of Presentation: 阪大豊中キャンパス
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Change of pH dependence of the enzyme activity by site-directed mutagenesis of Asn112 of thermolysin (2006)
  • Author(s): Kusano, M., Yasukawa, K., Hashida, Y., Inouye, K
  • Organizer: 58th Annual Meeting of the Japanese Biotechnology Society
  • Place of Presentation: Osaka University, Toyonaka
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Improvement of stability of thermolysisn by site-directed mutagenesis of the autolysis sites (2006)
  • Author(s): Murata, H., Matsumiya, Y., Inouye, K., Kubo, M
  • Organizer: 58th Annual Meeting of the Japanese Biotechnology Society
  • Place of Presentation: Osaka University, Toyonaka
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 順相分配高速液体クロマトグラフィーによるウロン酸分析. (2006)
  • Author(s): 垣田浩孝、上嶋洋、井上國世
  • Organizer: 2006年度日本食品分析研究会講演会
  • Place of Presentation: 東洋大学白山キャンパス
  • Year and Date: 2006-09-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] HPLC Analysis of uronic acids (2006)
  • Author(s): Kakita, H., Kamishima, H., Inouye, K
  • Organizer: 2006 Workshop of the Japan Food Analysis Society
  • Place of Presentation: Toyo University, Tokyo
  • Year and Date: 2006-09-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 熱安定性が低減したBacillus amyloliquefaciens α-アミラーゼの機能解析. (2006)
  • Author(s): 李承在, 毛利好希, 小根田洋史, 箕田正史, 井上國世
  • Organizer: 第6回食品酵素化学研究会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2006-09-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] キウイフルーツに含まれる抗カビ蛋白質の精製と構造解析. (2006)
  • Author(s): 森本康一, 井上國世
  • Organizer: 第6回食品酵素化学研究会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2006-09-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Characterization of Bacillus amyloliquefaciens a-amylase with lowered thermal stability (2006)
  • Author(s): Lee, S., Mouri, Y., Oneda, H., Minoda, M., Inouye, K
  • Organizer: 6th Forum of Food Enzyme Chemistry
  • Place of Presentation: Kinki University, School of Agriculture, Ikoma, Nara
  • Year and Date: 2006-09-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Purification and characterization of an antifungal protein of kiwi fruit (2006)
  • Author(s): Morimoto, K., Inouye, K
  • Organizer: 6th Forum of Food Enzyme Chemistry
  • Place of Presentation: Kinki University, School of Agriculture, Ikoma, Nara
  • Year and Date: 2006-09-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibitory effects of green tea catechins and lignans on the activity of human matrix metalloproteinase 7(MMP-7)and insfights into their structure-activity relationship. (2006)
  • Author(s): Inouye, K.
  • Organizer: 18th International Congress on Fibrinolysis and Proteolysis
  • Place of Presentation: San Diego Convention Center, San Diego, CA, USA
  • Year and Date: 2006-08-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Inhibitory effects of green tea catechins and lignans on the activity of human matrix metalloproteinase 7 (MMP-7) and insights into their structure-activity relationship (2006)
  • Author(s): Inouye, K
  • Organizer: 18th International Congress on Fibrinolysis and Proteolysis
  • Place of Presentation: San Diego Convention Center, San Diego, CA, USA
  • Year and Date: 2006-08-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Reaction mechanism of thermolysin and related metalloproteinases. (2006)
  • Author(s): Inouye, K.
  • Organizer: The Biological Chemistry Conference on Enzyme Reaction Mechanism
  • Place of Presentation: University of Michigan Medical Center, Ann Arbor, Michigan, USA
  • Year and Date: 2006-07-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Reaction mechanism of thermolysin and related metalloproteinases (2006)
  • Author(s): Inouye, K
  • Organizer: The Biological Chemistry Conference on Enzyme Reaction Mechanism
  • Place of Presentation: University of Michigan Medical Center, Ann Arbor, Michigan, USA
  • Year and Date: 2006-07-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Thermolysin, a thermophilic and halophilic metalloproteinase. (2006)
  • Author(s): Inouye, K.
  • Organizer: Canada-Japan Conference on Food Enzymes
  • Place of Presentation: University of Guelph, Guelph, Ontario, Canada
  • Year and Date: 2006-07-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Applications of high-performance Immuno affinity chromatography to proteomics and diagnosis. (2006)
  • Author(s): Inouye, K. and Nakamura, K.
  • Organizer: 20th IUBMB International congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress,
  • Place of Presentation: Kyoto International Conference Hall, Kyoto, Japan
  • Year and Date: 2006-06-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Applications of high-performance Immune affinity chromatography to proteomics and diagnosis (2006)
  • Author(s): Inouye, K., Nakamura, K
  • Organizer: 20th IUBMB International congress of Biochemistry and Molecular Biology and llth FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall, Kyoto
  • Year and Date: 2006-06-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Purification and characterization of thaumatin-like protein of kiwifruit. (2006)
  • Author(s): Morimoto, K., Ikegami, H., Kushida, T., Moriyama, H., and Inouye K.
  • Organizer: 20th IUBMB International congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress,
  • Place of Presentation: Kyoto International Conference Hall, Kyoto, Japan
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Purification and characterization of thaumatin-like protein of kiwifruit (2006)
  • Author(s): Morimoto, K. ; Ikegami, H., Kushida, T., Moriyama, H., Inouye, K
  • Organizer: 20th IUBMB International congress of Biochemistry and Molecular Biology and llth FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall, Kyoto
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Thermolysin, a thermophilic and halophilic metalloproteinase (2006)
  • Author(s): Inouye, K
  • Organizer: Canada-Japan Conference on Food Enzymes
  • Place of Presentation: University of Guelph, Guelph, Ontario, Canada
  • Year and Date: 2006-06-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ダイズたん白質7Sおよび11Sグロブリン画分のズブチリシン・カールスベルグによる消化性と凝集性の比較およびアレルゲンGly mBd 30Kの関与. (2006)
  • Author(s): 井上國世
  • Organizer: 不二たん白質研究振興財団第9回研究報告会
  • Place of Presentation: 千里阪急ホテル
  • Year and Date: 2006-06-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Digestivity of 7S and 11 globulin fractions in soyproteins by subtilisin Carlsberg, aggregation of the digests, and effect of allergen Gly m Bd 30K (2006)
  • Author(s): Inouye, K
  • Organizer: Fuji Protein Research Foundation 9th Symposium
  • Place of Presentation: Senri Hankyu Hotel, Suita
  • Year and Date: 2006-06-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] サーモライシンの活性部位のAsn112の変異による酵素活性への効果. (2006)
  • Author(s): 草野正雪, 保川清, 井上國世
  • Organizer: 第53回日本生化学会近畿支部例会
  • Place of Presentation: 滋賀医科大学
  • Year and Date: 2006-05-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Membrane-type serine protease 1のプロドメインの生物学的意義の解明. (2006)
  • Author(s): 兒島憲二, 都築巧, 伏木亨, 井上國世
  • Organizer: 第53回日本生化学会近畿支部例会
  • Place of Presentation: 滋賀医科大学
  • Year and Date: 2006-05-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of site-directed mutagenesis of Asn112 of thermolysin on its enzyme activity (2006)
  • Author(s): Kusano, M., Yasukawa, K., Inouye, K
  • Organizer: 53rd Kinki-branch Meeting of the Biochemical Society of Japan
  • Place of Presentation: Shiga Medical College, Ohtsu
  • Year and Date: 2006-05-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analysis of the biological role of the prodomain of membrane-type serine protease I (2006)
  • Author(s): Kojima, K., tsuzuki, T., Fushiki, T., Inouye, K
  • Organizer: 53rd Kinki-branch Meeting of the Biochemical Society of Japan
  • Place of Presentation: Shiga Medical College, Ohtsu
  • Year and Date: 2006-05-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] イネ新規ユビキチン様蛋白質RURM1のクローニング、大腸菌発現および精製. (2006)
  • Author(s): 築山拓司, 李鐘源, 井上國世, 奥本裕, 中崎鉄也, 谷坂隆俊
  • Organizer: 日本育種学会第109回講演会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2006-03-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cloning, expression in E. colt ; and purification of rice ubiquitin-like protein RURM1 (2006)
  • Author(s): Tsukiyama, T., Lee, J., Inouye, K., Okumoto, H., Nakazaki, T., Tanisaka, T
  • Organizer: 109th Symposium of the Japanese Plant Breeding Society
  • Place of Presentation: Tokyo University of Agriculture and Technology, Fuchu, Tokyo
  • Year and Date: 2006-03-10
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibitory activity of catechins and lignans against human matrix metalloproteinase-7 (MMP-7) ; Structure-activity relationship (2006)
  • Author(s): Inouye, K
  • Organizer: 65th Kyoto University Biosphere Research Institute Symposium
  • Place of Presentation: Kyoto University Biosphere Research Institute, Uji, Kyoto
  • Year and Date: 2006-03-03
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Enzymatic synthesis of aspartame and thermolysin enzymology. (2006)
  • Author(s): Inouye, K.
  • Organizer: The Japan-Korea Protease Conference
  • Place of Presentation: Gwanju Institute of Technology, Gwanju, Korea
  • Year and Date: 2006-02-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Proteolysis and metastasis. (2006)
  • Author(s): Inouye, K.
  • Organizer: The Japan-Korea Protease Conference
  • Place of Presentation: Gwanju Institute of Technology, Gwanju, Korea
  • Year and Date: 2006-02-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Enzymatic synthesis of aspartame and thermolysin enzymology (2006)
  • Author(s): Inouye, K
  • Organizer: The Japan-Korea Protease Conference
  • Place of Presentation: Gwanju Institute of Technology, Gwanju, Korea
  • Year and Date: 2006-02-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Proteolysis and metastasis (2006)
  • Author(s): Inouye, K
  • Organizer: The Japan-Korea Protease Conference
  • Place of Presentation: Gwanju Institute of Technology, Gwanju, Korea
  • Year and Date: 2006-02-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 組換えサーモライシンの大腸菌における菌体外生産精製並びに酵素的性質の解析 (2005)
  • Author(s): 保川清, 箕田正史, 滝田禎亮, 櫻間晴子, 橋田泰彦, 草野正雪, 井上國世
  • Organizer: 平成17年度日本生物工学会大会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2005-11-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Extracellular production of recombinant thermolysisn by E. coli, and its purification and enzyme chemical analysis (2005)
  • Author(s): Yasukawa, K., Minoda, M., Takita, T., Sakurama, H., Hashida, Y., Kusano, M., Inouye, K
  • Organizer: 2005 Annual Meeting of the Japanese Biotechnology Society
  • Place of Presentation: Tsukuba International Conference Hall
  • Year and Date: 2005-11-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ラットCYP1A1およびその変異体F240Aの発現大腸菌体によるダイオキシン類の代謝. (2005)
  • Author(s): 新京楽, 鎌倉昌樹, 生城真一, 太田美穂, 井上國世, 榊利之
  • Organizer: 第78回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2005-10-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 酵母で発現させたヒトCYP2W1の酵素特性 (2005)
  • Author(s): 笠井規行, 芳岡秀典, 新京楽, 鎌倉昌樹, 生城真一, 井上國世, 榊利之
  • Organizer: 第78回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2005-10-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Metabolism of dioxins by E. coil expressing rat CYP1A1 and its P240A mutant (2005)
  • Author(s): Shinkyo, R., Kamakura, M., Ikushiro, S., Ohta, M., Inouye, K., Sakaki, T
  • Organizer: 78th Annual Meeting of the Japan Biochemical Society
  • Place of Presentation: Kobe Internatyional Conference Ha
  • Year and Date: 2005-10-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Enzymatic properties of CYP2W1 expressed in yeast (2005)
  • Author(s): Kasai, N., Yoshioka, H., Kamakura, M., Ikushiro, S., Inouye, K., Sakaki, T
  • Organizer: 78th Annual Meeting of the Japan Biochemical Society
  • Place of Presentation: Kobe Internatyional Conference Ha
  • Year and Date: 2005-10-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The anomalous pH-dependence of human MMP-7 activity is controlled by the states of its tyrosyl residues: A chemical and protein modification study. (2005)
  • Author(s): Inouye, K. and Muta, Y.
  • Organizer: 4th General Meeting of the International Proteolysis Society associated with International Conference in Protease Inhibitors
  • Place of Presentation: Quebec City, Canada
  • Year and Date: 2005-10-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The anomalous pH-dependence of human MMP-7 activity is controlled by the states of its tyrosyl residues : A chemical and protein modification study (2005)
  • Author(s): Inouye, K., Muta, Y
  • Organizer: 4th General Meeting of the International Proteolysis Society associated with International Conference in Protease Inhibitors
  • Place of Presentation: Quebec City, Canada
  • Year and Date: 2005-10-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Differential expression of 11 PR-3 chitinase genes in rice(Oryzasativa L.)plants during rice blast infection and signal molecular treatments. (2005)
  • Author(s): Tsukiyama, T., Okumoto, Y., Inouye, K., Nakazaki, T., and Tanisaka, T.
  • Organizer: 6th Kyoto University International Symposium,
  • Place of Presentation: Chinese Academy of Agriculture, Beijin, China
  • Year and Date: 2005-10-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Differential expression of 11 PR-3 chitinase genes in rice (Oryza sativa L.) plants during rice blast infection and signal molecular treatments (2005)
  • Author(s): Tsukiyama, T., Okumoto, Y., Inouye, K., Nakazaki, T., Tanisaka, T
  • Organizer: 6th Kyoto University International Symposium
  • Place of Presentation: Chinese Academy of Agriculture, Beijin, China
  • Year and Date: 2005-10-08
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 蛍光法を用いるサーモライシン(TLN)の活性化機構の解析. (2005)
  • Author(s): 加茂昌之, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サーモライシンに対するアフィニティーリガンドとしてのGlu-D-Phe,Gly-L-Leu,D-Pheの評価. (2005)
  • Author(s): 草野正雪, 保川清, 中村孝司, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Membrane-type serine protease1(MT-SP1)の活性化機構の検討. (2005)
  • Author(s): 三宅由夏, 都築巧, 伏木亨, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Membrane-type serine protease 1(MT-SP1)プロドメインの生物学的意義の解明:urokinase-type plasminogen activator(uPA)の活性化における役割. (2005)
  • Author(s): 兒島憲二, 都築巧, 伏木亨, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] サーモライシン分子表面への変異導入による酵素活性への効果. (2005)
  • Author(s): 辰巳智香, 保川清, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Co^<2+>によるサーモライシン(TLN)の阻害メカニズムの解析. (2005)
  • Author(s): 橋田泰彦, 保川清, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 小麦由来α-アミラーゼ阻害剤の0.19AIによるPPAの阻害に対するNaClの効果. (2005)
  • Author(s): 李承在, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 順相クロマトグラフィーでのウロン酸の挙動. (2005)
  • Author(s): 垣田浩孝, 上嶋洋, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 大豆タンパク質のプロテアーゼ加水分解に伴う断片の凝集反応と熱処理の関係 (2005)
  • Author(s): 中野幹生, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] リジルtRNA合成酵素のL-リジンヒドロキサメートより生じたリジルアデニレート様複合体のX-線結晶構造解析. (2005)
  • Author(s): 櫻間晴子, 滝田禎亮, 三上文三, 伊藤貴文, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] リジルtRNA合成酵素の基質L-リジンの側鎖認識部位への変異導入によるアミノ酸基質に対する特異性改変. (2005)
  • Author(s): 阿波君枝, 滝田禎亮, 櫻間晴子, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] リジルtRNA合成酵素のクロスサブユニット間相互作用の変異導入解析. (2005)
  • Author(s): 猿渡由寛, 滝田禎亮, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cry45Aa組み換え体の大腸菌封入体から新規調製方法とその精製. (2005)
  • Author(s): 奥村史朗, 斎藤浩之, 片山秀樹, 日下芳友, 水城英一, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Streptomyces sp. REN-21由来有機溶媒耐性チロシナーゼの触媒特性と大腸菌での大量発現系の構築. (2005)
  • Author(s): 伊東昌章, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イネユビキチン様蛋白質RURM1の機能特性の解析および精製. (2005)
  • Author(s): 李鐘源, 谷坂隆俊, 築山拓司, 奥本裕, 中崎鉄也, 井上國世
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イネキチナーゼ遺伝子のいもち病特異的発現を制御する転写調節因子の同定. (2005)
  • Author(s): 築山拓司, 井上國世, 奥本裕, 寺地徹, 田中千尋, 中崎鉄也, 谷坂隆俊
  • Organizer: 日本農芸化学会2005年度関西・中四国・西日本支部合同大会
  • Place of Presentation: 大阪コンベンションセンター
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Activation mechanism of thermolysin using fluorescence (2005)
  • Author(s): Kamo, M., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analysis of the performance of afinity ligands, Glu-D-Phe, Gly-L-Leu, and D-Phe, for thermolysin (2005)
  • Author(s): Kusano, M., Yasukawa, K., Nakamura, K., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Activation mechanism of membrane-type serine proteasel(MT-SP1) (2005)
  • Author(s): Miyake, Y., Tsuzuki, T., Fushiki, T., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Biological role of the prodomain of membrane-type serine protease 1 (MT-SP1) (2005)
  • Author(s): Kijima, K., Tsuzuki, T., Fushiki, T., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of site-directed mutagenesis of thermolysin molecular surface on its enzyme activity (2005)
  • Author(s): Tatsurni, C., Yasukawa, K., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Inhibitory mechanism of Co2 against thermolysin (2005)
  • Author(s): Hashida, Y., Yasukawa, K., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of NaCI on the inhibitory activity of wheat 0.19AI against porcine pancreatic alpha-amylase (2005)
  • Author(s): Lee, S., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Chromatographic behaviors of uronic acids (2005)
  • Author(s): Kakita, T., Kamishima, H., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of heat treatment of soyprotein on the coagulation of its fragments produced by proteolysis (2005)
  • Author(s): Nakano, M., INouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] X-rau crystallographic analysis of lysyl-adenylate-like complex formed between lysyl-tRNA synthetase and L-lysine hydroxamate (2005)
  • Author(s): Sakurama, H., Takita, T., Mikami, B., Ito, T., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Conversion of the amino acid specificity of lysyl-tRNA synthetase by mutation at the L-Lys side-chain recognition site (2005)
  • Author(s): Awa, K., Takita, T., Sakurama, IL., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analysis of cross-subunit interaction of lysyl-tRNA synthetase by site-directed mutagenesis (2005)
  • Author(s): Saruwatari, Y., Takita, T., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A new preparation method of recombinant Cry45Aa and its purificationfrom E. coli inclusion body (2005)
  • Author(s): Okumura, S., Saito, H., Katayama, H., Kusaka, Y., Mizuki, E., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization of organic solvent resistant tyrosinase of Streptomyces sp. REN-21 and construction of its expression system by E. coli (2005)
  • Author(s): Ito, M., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characterization and purification of rice ubiquitin-like protein RURM1 (2005)
  • Author(s): Lee, J., Tanisaka, T., Tsukiyama, T., Okumoto, Y., Nakazaki, T., Inouye, K
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Transcription regulation factor of rice chitinase gene (2005)
  • Author(s): Tsukiyama, T., Inouye, K., Okumoto, Y., Teraji, T., Tanaka, C., Nakazaki, T., Tanisaka, T
  • Organizer: 2005 Joint Meeting of Kansai, Chushikoku, and Nishinippon Branches of the Nippon Nougeikagaku-kai
  • Place of Presentation: Osaka Convention Center
  • Year and Date: 2005-10-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Streptomyces griseolus由来CYP1051A1の酵素化学的性質の解明. (2005)
  • Author(s): 新京楽, 鎌倉昌樹, 生城真一, 井上國世, 榊利之
  • Organizer: 2005年度日本放線菌学会
  • Place of Presentation: ウイング高岡(富山県高岡市)
  • Year and Date: 2005-09-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] イネPR-3キチナーゼ遺伝子のいもち病感染特異的発現を制御する転写調節因子の解析. (2005)
  • Author(s): 築山拓司, 井上國世, 奥本裕, 寺地徹, 田中千尋, 中崎鉄也, 谷坂隆俊
  • Organizer: 日本育種学会2005年度大会
  • Place of Presentation: 筑波大学
  • Year and Date: 2005-08-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of transcription regulating factor of rice chichinase PR-3 gene (2005)
  • Author(s): Tsukiyama, T., Inouye, K., Okumoto, Y., Teraji, T., Tanaka, C., Nakazaki, T., Tanisaka, T
  • Organizer: 2005 Annual Meeting of the Japan Plant Breeding Society
  • Place of Presentation: Tsukuba University
  • Year and Date: 2005-08-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Enzyme chemical approach for optimization of enzyme immuno assay. (2005)
  • Author(s): Inouye, K.
  • Organizer: 2005 Biotechnology Annual Review Conference
  • Place of Presentation: University of Tromso, Tromso, Norway
  • Year and Date: 2005-07-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Enzyme chemical approach for optimization of enzyme immuno assay (2005)
  • Author(s): Inouye, K
  • Organizer: 2005 Biotechnology Annual Review Conference
  • Place of Presentation: University of Tromso, Tromso, Norw
  • Year and Date: 2005-07-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Anomalous pH-dependence of the activity of MMP-7 and the insights into the reaction mechanism of MMP-7. (2005)
  • Author(s): Inouye, K.
  • Organizer: 2005 Biotechnology Annual Review Conference
  • Place of Presentation: University of Tromso, Tromso, Norway
  • Year and Date: 2005-07-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Anomalous pH-dependence of the activity of MMP-7 and the insights into the reaction mechanism of MMP-7 (2005)
  • Author(s): Inouye, K
  • Organizer: 2005 Biotechnology Annual Review Conference
  • Place of Presentation: University of Tromso, Tromso, Nor
  • Year and Date: 2005-07-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 前立腺がんを標的としたプロホルモン型19-ノルビタミンD3誘導体の開発と、リガンド特異的なVDR-転写共役因子相互作用のHigh-Throughput Screeningシステムの開発. (2005)
  • Author(s): 荒井緑, 堤龍司, Chen, T. C., Holick, M. F., 榊利之, 漆野奈穂子, 井上國世, 伊藤沙耶, 武山健一, 加藤茂明, 橘高敦史
  • Organizer: 第3回次世代を担う有機化学シンポジウム
  • Place of Presentation: 長井記念ホール
  • Year and Date: 2005-05-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Development of pro-hormone-like 19-norvitamin D3 derivative and high-throughput screening system for DVR-transcription regulating factor (2005)
  • Author(s): Arai, M., Tsutsumi, R., Chen, T. C., Holick, M. F., Sakaki, T., Urushino, N., Inouye, K, Iyo, S., Takeyama, K., Kato, S., Kittaka, A
  • Organizer: 3rd Organic Chemistry Symposium for the Next Generation
  • Place of Presentation: Nagai Memorial Hall
  • Year and Date: 2005-05-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Membrane-type serine protease1活性化メカニズムの解析. (2005)
  • Author(s): 都築巧, 三宅由夏, 井上國世, 伏木亨
  • Organizer: 第60回日本栄養・食糧学会大会
  • Place of Presentation: 静岡県立大学
  • Year and Date: 2005-05-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Activation mechanism of membrane-type serine proteasel (2005)
  • Author(s): Tsuzuki, T., Miyake, Y., Inouye, K., Fushiki, T
  • Organizer: 60th Annual Meeting of the Japan Societyof Nutrition and Food
  • Place of Presentation: University of Shizuoka
  • Year and Date: 2005-05-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 基礎から学ぶ構造生物学(河野敬一、田之倉優、編) (2008)
  • Author(s): 井上國世(共著)
  • Total Pages: 91-78(203)
  • Publisher: 共立出版社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 酵素ハンドブック(第3版)(八木達彦、福井俊郎、一島英治、鏡山博行、虎谷哲夫、編) (2008)
  • Author(s): 井上國世(共著)
  • Total Pages: 1008
  • Publisher: 朝倉書店(印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Biotechnolog Annual Review, Volume 14(Ed. by M. R. El-Gewely) (2008)
  • Author(s): Okumura, S., Saitoh, H., Ishikawa, T., Mizuki, E., and Inouye, K(共著)
  • Publisher: Elsevier(印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Biotechnology Annual Review, Volume 13(Ed. by M. R. El-Gewely) (2007)
  • Author(s): Inouye, K., Kusano, M., Hashida, Y., Minoda, M., and Yasukawa, K.
  • Total Pages: 43-65
  • Publisher: Elsevier
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 20th IUBMB International Congress of Biochemistry and Molecular Biology Reports(Eds., Congre Co.; Taniguchi, N. and Suzuki, K.) (2007)
  • Author(s): Inouye, K(共著)
  • Total Pages: 65
  • Publisher: Congre
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 機能性糖質素材の開発と食品への応用(監修) (2006)
  • Author(s): 井上國世
  • Total Pages: 447
  • Publisher: シーエムシー出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 入門・酵素と補酵素の化学(翻訳) (2006)
  • Author(s): 井上國世
  • Total Pages: 320
  • Publisher: シュプリンガー・フェアラーク東京
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 機能性糖質素材の開発と食品への応用 (2005)
  • Author(s): 井上國世
  • Total Pages: 3-23
  • Publisher: シーエムシー出版
  • Description: 「研究成果報告書概要(和文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.enzchem.kais.kyoto-u.ac.jp
• [Patent(Industrial Property Rights)] 産業財産権の名称:微生物由来の菌体外プロテアーゼを生産するためのベクターおよびその利用. (2008)
  • Inventor(s): 井上國世、保川 清、草野正雪
  • Industrial Property Rights Holder: 京都大学
  • Patent Publication Number: 公開番号 特開2008-86226
  • Acquisition Date: 2008-04-17
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 産業財産権の名称:微生物由来の菌体外プロテアーゼを生産するためのベクターおよびその利用. (2006)
  • Inventor(s): 井上國世、保川 清、草野正雪
  • Industrial Property Rights Holder: 京都大学
  • Industrial Property Number: 出願番号 特願2006-268935
  • Filing Date: 2006-09-29
  • Description: 「研究成果報告書概要(和文)」より