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2006 Fiscal Year Final Research Report Summary

Study on Neutral Ceramidase involved in Sphingolipid Signaling

Research Project

Project/Area Number 17380068
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

ITO Makoto  Kyushu University, Bioscience and Biotechnology, Professor, 大学院農学研究院, 教授 (40253512)

Co-Investigator(Kenkyū-buntansha) ISHIDA Hideharu  Gifu University, Applied Biosciences, Professor, 応用生物学科, 教授 (20203002)
Project Period (FY) 2005 – 2006
Keywordsceramidase / ceramide / sphingosine / sphingosine-1-phosphate / zebrafish / embryogenesis / S1P signaling / early development
Research Abstract

Neutral ceramidases (CDases) are widely distributed from bacteria to humans. The bacteria and drosophila enzymes are exclusively secretory while the zebrafish and mammalian enzymes are typical type II integral membrane proteins which occasionally detach from cells after the processing of their NH2-terminal anchor. We found that the difference in the localization of the enzyme is entirely due to the presence of a mucin-like domain located downstream of the NH2-terminal signal/anchor sequence of the enzymes from vertebrates but not bacteria and invertebrates. Overexpression of mouse neutral CDase on the cell-surface or in the extracellular milieu but not in the ER significantly increased the intracellular concentration of sphingosine as well as sphingosine 1-phosphate (SIP) when the amount of cell-surface ceramide was increased by treatment of cells with bacterial sphingomyelinase. In contrast, knockdown of the CDase reduced the amount of both sphingolipid metabolites. The enzyme seems to participate in the generation of S1P in the vascular system in which cell-and lipoprotein-bound sphingomyelin are likely to be a source of ceramide. The knockdown of the neutral CDase impaired both S1P-mediated angiogenesis and the development of the heart during zebrafish embryogenesis, leading a lack of circulation of blood cells. Collectively, the enzyme is involved in the metabolism of ceramide at the plasma membranes and in the extracellular milieu, which could regulate the generation of SIP and S1P-mediated signaling through the production of sphingosine.

  • Research Products

    (13 results)

All 2007 2006

All Journal Article (12 results) Book (1 results)

  • [Journal Article] Ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa.2007

    • Author(s)
      N.Okino, M.Ito
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 6021-6030

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Endoglycoceramidase hydrolyzes oligogalactosylceramides to produce galactooligosaccharides and ceramides.2007

    • Author(s)
      Y.Ishibashi et al.
    • Journal Title

      Journal of Biological Chemistry 282

      Pages: 11386-11396

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Fucosyl-GMla, an endoglycoceramidase-resistant ganglioside of porcine brain.2007

    • Author(s)
      Xu Xu et al.
    • Journal Title

      Journal of Biochemistry 141

      Pages: 1-7

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Knock down of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase cause developmental abnormalities in zebrafish.2007

    • Author(s)
      T.Nimura et al.
    • Journal Title

      Arch. Biochem. Biophys. 457

      Pages: 205-216

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa.2007

    • Author(s)
      N.Okino, M.Ito
    • Journal Title

      J.Biol.Chem. 282

      Pages: 6021-6030

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A Novel Endoglycoceramidase hydrolyzes oligogalactosylceramides to produce galactooligosaccharides and ceramides.2007

    • Author(s)
      Y.Ishibashi, T.Nakasone, M.Kiyohara, Y.Horibata, K.Sakaguchi, A.Hijikata, S.Ichinose, A.Omori, Y.Yasui, A.Imamura, H.Ishida, M.Kiso, N.Okino, M.Ito
    • Journal Title

      J.Biol.Chem. 282

      Pages: 11386-11396

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ceramide/sphingosine/sphigosine 1-phosphate metabolism on the cell surface and in the extracellular space.2007

    • Author(s)
      M.Tani, M.Ito, Y.Igarashi
    • Journal Title

      CellSignal. 19

      Pages: 229-237

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fucosyl-GMla, an endoglycoceramidase-resistant ganglioside of porcine brain.2007

    • Author(s)
      X.Xu, H.Monjusho, M.Inagaki, Y.Hama, K.Yamaguchi, K.Sakaguchi, M.Iwamori, N.Okino, M.Ito
    • Journal Title

      J.Biochem. 141

      Pages: 1-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Knock down of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase cause developmental abnormalities in zebrafish. ino and M.Ito : Ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa.2007

    • Author(s)
      T.Nimura, N.Sueyoshi, A.Ishida, Y.Yoshimura, M.Ito, H.Tokumitsu, Y.Shigeki, N.Nozaki, I.Kameshita
    • Journal Title

      Arch.Biochem.Biophys. 457

      Pages: 205-216

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A novel phoshphatidylcholine which contains pentadecanoic acid at sn-1 and docosahexaenoic acid at sn-2 in Shizochytrium sp. F26-b.2006

    • Author(s)
      E.Abe et al.
    • Journal Title

      Journal of Biochimistry 140

      Pages: 247-253

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Structure or b-1,3-xylooligosaccharides generated from Caulerapa racemosa var. laete-vierens β-1,3-xylana by the action of b-1-3-xylanase.2006

    • Author(s)
      M.Kiyohara et al.
    • Journal Title

      Journal of Biochemistry 1402

      Pages: 369-373

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A Novel Phosphatidylcholine Whitch Contains Pentadecanoic Acid at sn-1 and Docosahexaenoic Acid at sn-2 in Schizochytrium sp.F26-b.2006

    • Author(s)
      E.Abe, Y.Hayashi, Y.Hama, M.Hayashi, M.Inagaki, M.Ito
    • Journal Title

      J.Biochem. 140

      Pages: 247-253

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Sphingolipid Biology2006

    • Author(s)
      M.Ito et al.分担
    • Total Pages
      531(183-196)
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2008-05-27  

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