2007 Fiscal Year Final Research Report Summary
Sponge-derived neurotoxic substances : Structure, Bioactivity, and their cellular localization.
| Project/Area Number |
17380125
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | Kitasato University |
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Principal Investigator |
SAKAI Ryuichi Kitasato University, Fisheries Sciences, Professor (20265721)
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| Co-Investigator(Kenkyū-buntansha) |
JINBO Mitsuru Kitasato University, Fisheries Sciences, Associate Professor (10291650)
KOIKE Kazuhiko Hiroshima University, Applied Biological Sciences, Associate Professor (30265722)
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| Project Period (FY) |
2005 – 2007
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| Keywords | glutamate receptors / neurotoxin / sponge / biological activity / natural product chemistry / polyamine / peptide / biomineralization |
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| Research Abstract |
In the present study, we identified new polyamines from a Okinawan sponge Axinissa aculeate that modulate ligand binding to NMDA type glutamate receptors. These polyamines may also function as a factor involved in silica biomineralization in the sponge. From the same extract, we identified new peptides containing polyamine in the substructure and named aculeines. This novel highly modified peptide showed strong convulsant actions in mice. We also isolated Cribropurine, a new purine from a Micronesian sponge Chribrocharina olemda. Cribroprine elicited a seizure-like spontaneous current in rat hippocampal neuron. A lipophilic extract of the sponge Raspailia sp. contained raspailol as seizure-inducing factor in mice. Okinawan sponge Spongosolites afforded an isolation of protein toxin Soritesidine. A terminal amino acid sequence for sotitesidine was determined We identified a cell where an excitatory amino acid dysiherbaine was localized. The cell was identified to be a cyanobacteria Synechosystis sp based on its 16S rDNA sequence This result suggested that dysiherbaine was a cyanobacterial product This species also contained 6-phospholylated deoxynojirimycin derivatives. Investigation of an aqueous extract that exhibited AMPAKine like activity resulted in separation of bioactive fractions. One of the active fractions was shown to contain lectin based on its hemagglutinating activity. The lectin- containing fraction showed AMPAKine-like activity whereas the fraction containing small molecule was responsible for seizure-induction in mice. The preset study demonstrated that toxic extracts that induce convulsion in mice is a interesting source of neuron active compounds that can be useful tools in neurobiology.
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Research Products
(26 results)
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[Journal Article] Cellular Origin of Dysiherbaine an Excitatory Amino Acid Derived from a Marine Sponge2008
Author(s)
Sakai, R., Yoshida, K., Kimura, A., Koike, K., Jimbo, M., Koike, K., Kobiyama, A., Kamiya, H
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Journal Title
ChemBioChem 9
Pages: 543-551
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Chemical Biology of Neuroactive Spone Metabolites2007
Author(s)
Sakai, R., Matsunaga, S., Kimura, A., Koike, K., Koike, K., Jimbo, M., Kamiya, H
Organizer
VI^<th> US-Japan Seminar : Frontiers in Marine Natural Products Research
Place of Presentation
Park City Utah, U. S. A
Year and Date
20070701-06
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Cellular localization of excitatory amino acid dysiherbaine2007
Author(s)
Atsuko, Kimura, Ryuichi, Sakai, Kazufumui, Yoshida, Kanae, Koike, Atsushi, Kobiyama, Mitsuru, Jimbo, Hisao, Kamiya
Organizer
The Japanese Society of Fisheries Sciences, 2007 annual meeting
Place of Presentation
Tokyo, University of Marine Science and Technology
Year and Date
2007-04-01
Description
「研究成果報告書概要(欧文)」より
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