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2008 Fiscal Year Final Research Report

The development of methods for evaluating embryo qualitybased on the electrochemical measuring technique

Research Project

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Project/Area Number 17380164
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionYamagata University (2008)
Tohoku University (2005-2007)

Principal Investigator

ABE Hiroyuki  Yamagata University, 大学院・理工学研究科, 准教授 (10375199)

Co-Investigator(Kenkyū-buntansha) HITOSHI Shiku  東北大学, 大学院・環境科学研究科, 准教授 (10361164)
YOKOO Masaki  東北大学, 未来医工学治療開発センター, 助教 (10396541)
Project Period (FY) 2005 – 2008
Keywords電気化学計測 / 細胞機能評価 / 細胞呼吸 / ミトコンドリア / 体外受精
Research Abstract

本研究では、電気化学的イメージング技術を基盤とする細胞呼吸計測システムを開発し、これを応用した受精卵品質評価法の開発を目的とした。走査型電気化学顕微鏡をベースに、非侵襲的・高感度で家畜胚や卵子の呼吸量を測定できる「細胞呼吸計測システム」の開発に成功した。この計測システムを用い、呼吸活性を指標に品質の高い家畜受精卵を選別できる新しい評価法を確立した。

  • Research Products

    (18 results)

All 2008 2007 2006 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (6 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Morphometric and ultrastructural changes in ciliated cells of the oviductal epithelium in prolific Chinese Meishan and Large White pigs during the estrous cycle2008

    • Author(s)
      Abe H., Hoshi H.
    • Journal Title

      Reprod Domest Anim 43

      Pages: 66-73

    • Peer Reviewed
  • [Journal Article] Influence of hyaluronan accumulation during cumulus expansion on in vitro porcine oocyte maturation2008

    • Author(s)
      Yokoo M., Kimura N., Abe H., Sato E.
    • Journal Title

      Zygote 16

      Pages: 309-314

    • Peer Reviewed
  • [Journal Article] Analysis of mRNA from single adherent cells and spheroids collected using fast electrical lysis2007

    • Author(s)
      Nashimoto Y., Takahashi Y., YamakawaT., Torisawa Y., Yasukawa T., Ito-SasakiT., Yokoo M., Abe H., Shiku H., Kambara H., Matsue T.
    • Journal Title

      Anal.Chem. 79

      Pages: 6823-6830

    • Peer Reviewed
  • [Journal Article] Excess tyrosine rescues the reduced activity ofproliferation and differentiation of cultured recessive yellow melanocytes derived from neonatal mouse epidermis2007

    • Author(s)
      Hirobe T., Abe H., Wakamatsu K., Ito S.,Kawa Y., Soma Y., Mizoguchi M.
    • Journal Title

      Eur J Cell Biol 86

      Pages: 315-330

    • Peer Reviewed
  • [Journal Article] Regional and cyclic variations in the ultrastructural features of secretory cells in the oviductal epithelium of the Chinese Meishan pig2007

    • Author(s)
      Abe H., Hoshi H.
    • Journal Title

      Reprod Domest Anim 42

      Pages: 292-298

    • Peer Reviewed
  • [Journal Article] A microfluidic chip integrated with amperometric detector array for in situ estimating oxygen consumption characteristics of single bovine embryo2007

    • Author(s)
      Wu C.-C., Saito T., Yasukawa T., Shiku H., Abe H., Hoshi H., Matsue T.
    • Journal Title

      Sens Actuat B 125

      Pages: 680-687

    • Peer Reviewed
  • [Journal Article] Oxygen consumption of cell suspension in a poly(dimethlsiloxane)(PDMS)microchannel estimated by scanning electrochemical microscopy2006

    • Author(s)
      Saito T., Wu C.-C., Shiku H., Yasukawa T., Yokoo M., Sasaki T., Abe H., Hoshi H.,Matsue T.
    • Journal Title

      Analyst 131

      Pages: 1006-1011

    • Peer Reviewed
  • [Presentation] 新しい培養評価法-受精卵呼吸解析による培養液の性能評価について-2008

    • Author(s)
      阿部宏之
    • Organizer
      第49回日本哺乳動物卵子学会
    • Place of Presentation
      名古屋国際会議場(招待講演)
    • Year and Date
      20080500
  • [Presentation] Evaluating the quality of single human embryos with a non-invasive and highly sensitive measurement of respiration activity by scanning electrochemical microscopy2008

    • Author(s)
      Abe H
    • Organizer
      The 3rd International Workshop on Approaches to Single-Cell-Analysis
    • Place of Presentation
      Zurich, Switzerland(invited)
    • Year and Date
      2008-09-11
  • [Presentation] 電気化学計測法による卵子呼吸能解析とクオリティー評価への応用2007

    • Author(s)
      阿部宏之
    • Organizer
      第52回日本生殖医学会
    • Place of Presentation
      秋田県民会館(招待講演)
    • Year and Date
      20071000
  • [Presentation] 呼吸代謝能解析による卵子・胚の品質評価と応用2007

    • Author(s)
      阿部宏之
    • Organizer
      第25回日本受精着床学会
    • Place of Presentation
      仙台国際センター(招待講演)
    • Year and Date
      20070800
  • [Presentation] Estimation of respiratory activity of single human embryos by scanning electrochemical microscopy2007

    • Author(s)
      Abe H
    • Organizer
      The 5th International Forum on Post-genome Technologies
    • Place of Presentation
      Suzhou, China(invited)
    • Year and Date
      2007-10-11
  • [Presentation] Non-invasive quality assessment of IVP embryos Post Conference Satellite Symposium2007

    • Author(s)
      Abe H
    • Organizer
      The 33^<rd> Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Kyoto, Japan(invited)
    • Year and Date
      2007-01-10
  • [Book] 「マイクロ電極を用いた受精卵呼吸測定」、動物実験代替のためのバイオマテリアル開発2007

    • Author(s)
      阿部宏之
    • Total Pages
      269-276
    • Publisher
      シーエムシー出版
  • [Book] 胚の呼吸能の測定、図説よくわかる臨床不妊症学入門・生殖補助医療編2007

    • Author(s)
      阿部宏之
    • Total Pages
      50-59
    • Publisher
      中外医学社
  • [Remarks] 2007年11月22日朝日新聞「不妊治療で元気な受精卵選別」

  • [Remarks] 2009年1月15日山形新聞「元気な受精卵は呼吸が活発・不妊治療の成績向上に期待」

  • [Patent(Industrial Property Rights)] 哺乳動物受精卵の呼吸活性測定方法及びその電気化学デバイス2008

    • Inventor(s)
      珠玖仁、末永智一、高野真一郎、伊達安基、齋藤剛史、横尾正樹、伊藤隆広、阿部宏之
    • Industrial Property Rights Holder
      クリノ(株)、東北大学、山形大学
    • Industrial Property Number
      特願2008-293117
    • Filing Date
      2008-11-15

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Published: 2010-06-10   Modified: 2016-04-21  

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