2007 Fiscal Year Final Research Report Summary
Explorations and Functional Analysis of gene related with fat accumulation
| Project/Area Number |
17380168
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
ROH Sanggun Shinshu University, Faculty of Agriculture, Associated professor (90322130)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SASAKI Shin-ichi Shinshu University, Faculty of Agriculture, Professor (00003682)
ONO Tamao Shinshu University, Faculty of Agriculture, Professor (10177264)
|
|---|
| Project Period (FY) |
2005 – 2007
|
| Keywords | Bovine / Adipogenesis / Adipogenin / GPCR43 / GPCR120 |
|---|
| Research Abstract |
In order to build the system of good beef production of Japanese Black Cattle, understanding of fat accumulation among each adipose tissue is important. Therefore, the candidate genes related with fat depots have investigated in Japanese Black Cattle. (1) The gene of CDC42, prefoldin-5, decorin, phosphate carrier, 12S ribosomal RNA gene, and Kbtbd2 was identified by the analysis of the gene expression profile between subcutaneous and visceral adipose tissue using the differential display RT-PCR method. Expression patterns of these genes in Japanese Black Cattle were different with those of a single stomach animal like a pig or a mouse. (2) GPCR43, Adipogenin and GPCR120 were highly expressed in adipose tissue and up-regulated during adipocyte differentiation. GPCR43, Adipogenin, GPCR120, and Claudin-6 were important genes on adipocyte differentiation and adipose tissue development (4) Bovine Adipogenin isoform cDNA received alternatives splicing as a result of base sequence analysis, and it was confirmed that the base sequence is missing as compared with Adipogenin. Bovine isoform cDNA was highly expressed in adipose tissue and up-regulated during the adipocyte differentiation of bovine. This was similar with the results of the Adipogenin gene. A possibility that the isoform of an Adipogenin gene was involved with adipose tissue developments was suggested. (5) Introducing an Adipogenin gene into a C2C12 cell showed the fat accumulation in cells without the changes of genes related with adipogenesis.
|
Research Products
(76 results)
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Chemerin-A New adipokine that modulates adipogenesis via its own receptor2007
Author(s)
Roh, SG., Song, SH., Choi, KC., Katoh, K., Wittamer, V., Parmentier, M., Sasaki, S
-
Journal Title
Biochem. Biophys. Res. Commun 362(4)
Pages: 1013-18
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] The possibility of the involvement of transient Receptor Potential Vanillois (TRPV) 4 in mouse sperm thermotaxis2007
Author(s)
Kawanishi, T., Takahashi, H., Funai, S., Suzuki, M., Mizuno, A., Goto, C., Roh, SG., Sasaki, S., Tsuji, H., Hamano, K
-
Journal Title
Hokushinetsu Journal of Animal Science 94
Pages: 35-41
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Expression of FSH receptor mRNA in Goat seminiferous Epithelium2007
Author(s)
Takahashi, H., Funai, S., Kawanishi, T., Goth, C., Rob, SG., Ono, T., Sasaki, S., Tsuji, H., Nakura, Y., Fujita, M., Hamano, K
-
Journal Title
Hokushinetsu Journal'of Animal Science 94
Pages: 43-47
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Roh SG (Corresponding Author), The regulation of adipogenesis through GPR1202007
Author(s)
Gotoh, C., Hong, YH., Iga, T., Hishikawa, D., Suzuki, Y., Song, SH., Choi, KC., Adachi, T., Hirasawa, A., Tsujimoto, G., Sasaki, S
-
Journal Title
Biochem. Biophys. Res. Commun 354(2)
Pages: 591-597
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Identification of differentially expressed transcripts in bovine rumen and abomasum using a differnutial display method2007
Author(s)
Roh, SG., Kuno, M., Hishikawa, D., Hong, YH., Katoh, K., Ohara, Y., Hidari, H., Sasaki, S
-
Journal Title
J. Anim. Sci 85
Pages: 395-403
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Z-chromosome specific primers for chicken-quail hybrid blastoderm2007
Author(s)
Takagi, S., Ono, T., Tsukada, A., Atsumi, Y., Mizushima, S., Saito, N., Shimada, K
-
Journal Title
Journal of Poultry Science 44(2)
Pages: 209-212
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken2007
Author(s)
Yamamoto, Y., Usui, F., Nakamura, Y., Ito, Y., Tagami, T., Nirasawa, K., Matsubara, Y., Ono, T., Kagami, H
-
Journal Title
Biology of Reproduction 77(1)
Pages: 115-119
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome2007
Author(s)
Takagi, S., Ono, T., Tsukada, A., Atsumi, Y., Mizushima, S., Saito, N., Shimada, K
-
Journal Title
Poultry Science 86(5)
Pages: 937-943
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Migration and proliferation of primordial germ cells in the early chicken embryo2007
Author(s)
Nakamura, Y., Yamamoto, Y., Mushika, T., Usui, F., Ono, T., Seitoko, AR., Takeda, K., Nirasawa, K., Kagami, H., Tagami, T
-
Journal Title
Poultry Science 86(10)
Pages: 2182-2193
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Differential susceptibility of rat embryos to methyl methanesulfonate during the pregastrulation period2007
Author(s)
Yokoi, R., Suda, S., Kobayashi K., Kuroda, J., Kusama, H., Kagami, H., Ono, T
-
Journal Title
Journal of Toxicological Sciences 32(5)
Pages: 495-503
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
[Journal Article] Production of lean beef containing a high content of trans-10, cis-12 conjugated linoleic acid by feeding high-temperature-micro-time treated diet with extruded soybean2006
Author(s)
Xu, CX., Lee, HG., Lee, BK., Wang, JH., Hong, ZS., Kim, TG., Kang, SK., Choi, NG., Roh, SG., Choi, Y.J
-
Journal Title
Biosci Biotechnol. Biochem 70(11)
Pages: 2589-2597
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Molecular cloning and expression analysis of cDNA encoding bovine adipogenin2006
Author(s)
Hong, YH., Ogihara, Y., Hishikawa, D., Gotoh, C.,Iga, T., Suzuki, Y., Song, SH., Nakajima, K., Kozakai, T., Sasaki, S., Roh, SG
-
Journal Title
Animal Science Journal 77
Pages: 613-619
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] The response of plasma leptin and feed intake to growth hormone administration in Holstein calves with different planes of nutrition2006
Author(s)
Lee, HG., Hong, ZS., Kim, MK., Kang, SK., Xu, CX., Cho, JS., Seo, KS., Roh, SG., Choi, YJ
-
Journal Title
Can. J. Anim. Sci 86
Pages: 225-233
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Reduction in voltage-gated K+ currents in primary cultured rat pancreatic β-cells by linoleic acids2006
Author(s)
Feng, DD., Luo, Z., Roh, SG., Hernandez, M., Tawadoros, N., Chen, C
-
Journal Title
Endocrinology 147
Pages: 674-682
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Selective decrease of chick embryonic primordial germ cells in vivo and in vitro by soft X-ray irradiation2006
Author(s)
Lim, JM., Kwon, HM., Kim, DK., Kim, JN., Park, TS., Ono, T., Han, JY
-
Journal Title
Animal Reproduction Science 95(1-2)
Pages: 67-74
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
[Journal Article] Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR432005
Author(s)
Hong, YH., Nishimura, Y., Hishikawa, D., Tsuzuki, H., Miyahara, H., Gotoh, C., Choi, KC., Fung, D., Chen, C., Lee, HG., Katoh, K., Roh, SG., Sasaki, S
-
Journal Title
Endocrinology 146
Pages: 5092-5099
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Up-regulation of the claudin-6 gene in adipogenesis2005
Author(s)
Hong, YH., Hishikawa, D., Miyahara, H., Nishimura, Y., Tsuzuki, H., Gotoh, C., Iga, T., Suzuki, Y., Song, SH., Choi, KC., Lee, HG., Sasaki, S., Roh, SG., (Corresponding Author)
-
Journal Title
Biosci. Biotechnol. Biochem 69(11)
Pages: 2117-2121
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Identification of genes expressed differentially in subcutaneous and visceral fat of cattle, pig and mouse2005
Author(s)
Hishikawa, D., Hong, YH., Roh, SG., Miyahara, H., Nishimura, Y., Tomimatsu, A., Tsuzuki, H., Gotoh, C., Kuno, M., Choi, KC., Lee, HG., Cho, KK., Hidari, H., Sasaki, S
-
Journal Title
Physiological Genomics 21(3)
Pages: 343-50
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Altered cytokine expression in mesenteric lymph nodes in a rat strain (MES) that spontaneously develops hypereosinophilia2005
Author(s)
Muto, S., Monnai, M., Okuhara, Y., Murakami, M., Kuroda, J., One, T., Matsumoto, K
-
Journal Title
Immunology 116(3)
Pages: 373-380
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis2005
Author(s)
Hong, YH., Hishikawa, D., Miyahara, H., Tsuzuki, H., Nishimura, Y., Gotoh, C., Choi, KC., Hokari, Y., Takagi, Y., Lee, HG., Cho, KK., Roh, SG., Sasaki, S
-
Journal Title
Molecular and Cellular Biochemistry 276
Pages: 133-141
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Inhibition of GH secretagogue receptor antagonist, [D-Lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes2005
Author(s)
Roh, SG., Hong, YH., Hishikawa, D., Tsuzuki, H., Miyahara, H., Nishimura, Y,. Gotoh, C., Suzuki, Y., Choi, KC., Lee, HG., Sasaki, S
-
Journal Title
Animal Science Journal 76
Pages: 381-386
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
