2007 Fiscal Year Final Research Report Summary
Comparative studies on beta amyloid deposition and brain aging
| Project/Area Number |
17380185
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAYAMA Hiroyuki The University of Tokyo, Graduate School of Agricltural and Life Sciences, Professor (40155891)
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| Co-Investigator(Kenkyū-buntansha) |
MANABE Noboru The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor (80243070)
UETSUKA Koji The University of Tokyo, Chief scientist (60251419)
UCHIDA Kazuyuki The University of Tokyo, Graduate School of Agricultural and life Sciences, Associate professor (10223554)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Dog / Cat / Microarray / Nprilysin / Beta amyloid(A beta) / MPTP / Brain / Prion model |
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| Research Abstract |
We first investigated total gene expressions in the canine brain using a microarray system. Genes upregulated were GFAP, CNPase and p35, and those downregulated were APP and Tau.
Activities "and expressions of neprilysin (Nep) and depositions of beta amyloid (A beta) were examined in the canine and feline brains. Nep was weakly expressed in the cerebral cortex and strongly in the striatum and substantia nigra (SN) in both animals. Activities of Nep were higher in the order of striatum > cerebral cortex > white matter / hippocampus. Nep activity and expression were not related to aging, although Nep activity was tended to decrease in the canine striatum with age. A beta deposition was increased with age in both dogs and cats.
Strain difference of the neuronal cell loss was investigated in MPTP-treated C57BL/6 mice which is a Parkinson's disease model. Tyrosine hydroxylase (TH) expression in the SN-striatum was decreased in C57BL/6 mice but not in BALB/c mice. Some mice showed such decrease of HT expression in non-inbred ICR mice. It was therefore suggested that a single gene would be involved in the expression of the disease in the MPTP-treated Parkinson model mice.
Fusion protein of green fluorescent protein (GFP) and A beta has been massively produced. The GFP-A beta was administered orally into juvenile mice and cows before or after weaning. GFP-A beta reached to the jejunum and ileum without degradation in the stomach, and incorporated through the absorptive epithelium in the villi of both species. The incorporation occurred in the suckling period. This experimental system would be an excellent model to investigate the intestinal invasion of prion protein.
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[Journal Article] Complementary distributions of amyloid-beta and neprilysin in the brains of dogs and cats.2008
Author(s)
Takeuchi, Y, Uetsuka, K, Murayama, M., Kikuta, F., Takashima, A., Doi, K., and Nakayama, H.
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Journal Title
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Incorporation of beta-amyloid protein through the bovine ileal epithelium before and after weaning: a model for orally transmitted amyloidosis.2008
Author(s)
Ano, Y., Nakayama, H., Sakai, Y., Sakudo, A., Endo, M., Ebisu, S., Li, Jun You, Manabe, N., and Onodera, T.
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Journal Title
Microbiol. Immunol. 52(印刷中)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Complementary distributions of amylod-beta and neprilysin in the brains of dogs and cats2008
Author(s)
Takeuchi, Y., Uetsuka, K., Murayama, M., Kikuta, F., Takashima, A., Doi, K., Nakayama, H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Incorporation of beta-amyloid protein through the bovine ileal epithelium before and after weaning : a model for orally transmitted amyloidosis2008
Author(s)
Ano, Y., Nakayama, H. Sakai, Y., Sakudo, A., Endo, M., Ebisu, S., Li, Jun You, Manabe, N., Onodera, T.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Two cases of feline malignant craniophalingioma.2005
Author(s)
Nagata, T., Nakayama, H., Uchida, K., Uetsuka, K., Yasoshima, A., Yasunaga, S., Masuda, K., Tsujimoto, H., Kuwajima, E., Nishimura, R., Sassaki, N. and Doi, K.
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Journal Title
Vet. Pathol. 42
Pages: 663-665
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Two cases of feline malignant cranipophalingioma2005
Author(s)
Nagata, T., Nakayama, H., Uchida, K., Uetsuka, K., Yasoshima, A., Yasunaga, S., Masuda, K., Tsujimoto, H., Kuwajima, E., Nishimura, R., Sassaki, N. Doi, K.
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Journal Title
Vet. Pathol 42
Pages: 663-665
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Incorporation of amyloid protein in the intestines of weaning mice and cows2008
Author(s)
Ano, Y., Nakayama, H., Sakudo, S., Manabe, N., Onodera, T.
Organizer
145th Meeting of the Japanese Society of Veterinary Sciences
Place of Presentation
Sagamihara, Kanagawa
Year and Date
20080300
Description
「研究成果報告書概要(欧文)」より
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