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2006 Fiscal Year Final Research Report Summary

Studies on clinical relevance of antigen-specific immunoglobulin production from mast cells

Research Project

Project/Area Number 17380189
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Clinical veterinary science
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MASUDA Kenichi  The Institute of Physical and Chemical Research, Laboratory for Vaccine Design, Researcher (40313077)

Co-Investigator(Kenkyū-buntansha) SAKAGUCHI Masahiro  Chiba University, Graduate School Medicine, Associate Professor (20170590)
TSUJIMOTO Hajime  The University of Tokyo, Graduate School of Agricultural and Life Science, Professor (60163804)
MATSUDA Hiroshi  Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Professor (80145820)
KURATA Keigo  RIKEN, Laboratory for Vaccine Design, Researcher (50391941)
Project Period (FY) 2005 – 2006
Keywordsmast cells / IgE / dogs / animal model
Research Abstract

We found that dogs can be an alternative animal model of mice for investigation of allergic diseases such as type I hypersensitivity. In order to investigate allergic reaction, we established canine mast cell line as a powerful tool for the study. We also clarified allergic reaction associated with mast cells, such as endothelin and TARC related reactions. A novel major allergen of Japanese cedar pollen, Cry j 3, was reported to induce IgE production. Using the mast cell line, we investigated that IgE production from mast cells were investigated with flow cytometory, western blotting, immunohistochemistry, and quantitative PCR analysis, finding IgE protein but not mRNA of canine IgE gene. Similarly, using mouse bone-marrow derived cultured mast cells, those studies were carried out, however, IgE protein production was found, but not IgE gene.
In summary, we have investigated that allergic reactions related with IgE and mast cells and provided the cell line and animal models as well as useful information for research. Those results will provide a further possibility of the research to clarify the pathogenesis.

  • Research Products

    (18 results)

All 2007 2005

All Journal Article (18 results)

  • [Journal Article] DNA vaccination against Japanese cedar pollinosis in dogs lesionall suppresses type I hypersensitivity by controlling lesional mast cells2007

    • Author(s)
      Masuda, K.
    • Journal Title

      Veterinary Immunology and Immunopathology 108

      Pages: 185-187

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation and characterization of native Cry j3from Japanese cedar(Cryptomeria japonica)pollen2007

    • Author(s)
      Fulimura, T., et. al.
    • Journal Title

      Allergy 62

      Pages: 547-553

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of bovine serum albumin as an IgE-reactive beef component in a dog with food hypersensitivity against beef2007

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Journal of Veterinary Medical Science 69

      Pages: 865-877

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunoblot analysis for IgE-reactive components of fetal calf serum in dogs that developed allergic reactions after non-rabies vaccination2007

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Veterinary Immunology and Immunopathology 115

      Pages: 166-171

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A blinded randomized controlled trial evaluating the usefulness of a novel diet(aminoprotect care)in dogs with spontaneous food allergy2007

    • Author(s)
      Olivry, T., et. al.
    • Journal Title

      Journal of Veterinary Medical Science 69

      Pages: 1025-1031

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] DNA vaccination against Japanese cedar pollinosis in dogs suppresses type I hypersensitivity by controlling lesional mast cells.2007

    • Author(s)
      Masuda, K.
    • Journal Title

      Veterinary Immunology and Immunopathology 108

      Pages: 185-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isolation and characterization of native Cry j 3 from Japanese cedar(Cryptomeria japonica) pollen.2007

    • Author(s)
      Fujimura, T., et. al.
    • Journal Title

      Allergy 62

      Pages: 547-53

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Identification of bovine serum albumin as IgE-reactive beef component in a dog with food hypersensitivity against beef.2007

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Journal of Veterinary Medical Science 69

      Pages: 865-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunoblot analysis for IgE-reactive components of fetal calf serum in dogs that developed allergic reactions after non-rabies vaccination.2007

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Veterinary Immunology and Immunopathology 115

      Pages: 166-71

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A blinded randomized controlled trial evaluating the usefulness of a novel diet(aminoprotect care) in dogs with spontaneous food allergy.2007

    • Author(s)
      Olivry, T., et. al.
    • Journal Title

      Journal of Veterinary Medical Science 69

      Pages: 1025-31

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Suspected allergic reactions after vaccination in 85 dogs in Japan2005

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Veterinary Record 156

      Pages: 87-88

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] IgE reactivity to vaccine components in dogs that developed immediate-type allergic reactions after vaccination2005

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Veterinary Immunology and Immunopathology 104

      Pages: 249-256

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Production of a monoclonal antibody to canine thymus and activation-regulated chemokine(TARC)and detection of TARC in lesional skin from dogs with atopic dermatitis2005

    • Author(s)
      Maeda, T., et. al.
    • Journal Title

      Veterinary Immunology and ImmunoPathology 103

      Pages: 83-92

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cloning of cDNA encoding canine endothelin receptors and their expressions in normal tissues2005

    • Author(s)
      Yasuda, N., et. al.
    • Journal Title

      Journal of Veterinary Medical Science 67

      Pages: 1075-1079

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Suspected allergic reactions after vaccination in 85 dogs in Japan.2005

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Veterinary Record 156

      Pages: 87-88

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] IgE reactivity to vaccine components in dogs that developed immediate-type allergic reactions after vaccination.2005

    • Author(s)
      Ohmori, K., et. al.
    • Journal Title

      Veterinary Immunology and Immunopathology 104

      Pages: 249-56

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Production of a monoclonal antibody to canine thymus and activation-regulated chemokine(TARC) and detection of TARC in lesional skin from dogs with atopic dermatitis.2005

    • Author(s)
      Maeda, T., et. al.
    • Journal Title

      Veterinary Immunology and immunopathology 103

      Pages: 83-92

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning of cDNA encoding canine endothelin receptors and their expressions in normal tissues.2005

    • Author(s)
      Yasuda, N., et. al.
    • Journal Title

      Journal of Veterinary Medical Science 67

      Pages: 1075-9

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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