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2006 Fiscal Year Final Research Report Summary

Mechanism for the genesis of new neurons in adult mammalian brain

Research Project

Project/Area Number 17380199
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionThe University of Tokyo

Principal Investigator

HISATSUNE Tatsuhiro  The University of Tokyo, Graduate School of Frontier Sciences, Associate Professor (10238298)

Project Period (FY) 2005 – 2006
KeywordsNeural Stem Cells / Learning / GABA / Hippocampus / Adult / Theta rhythm / Calcium / Imaging
Research Abstract

Adult neurogenesis occurs in the dentate gyrus of hippocampus. A series of reports have suggested that the process of this neuronal differentiation can be regulated by the hippocampal network activities. New neurons are generated from sequential differentiation from radial-glia-like stem/progenitor cell (type-1 cell) following the generation of transiently amplifying progenitor cell (type-2 cell). In this study, we found that type-2 cell receives GABAergic inputs by means of targeted-patch clump recordings of progenitor cells in a fresh hippocampal slice from nesin-GFP mice, in combination with an immunohistochemical (con-focal and EM) analysis.
Due to the elevated [C1-]i in type-2 cell, the GABAergic activity through GABAA receptor depolarized the hippocampal progenitor. This excitation induced the elevation of[Ca^<2+>]i, and then promoted the expression of NeuroD, a positive regulator for neuronal commitment. A BrdU-pulse labeling study using living animals with the injections of GABAA receptor agonists and antagonists clearly showed that this excitatory GABAergic input on type-2 cell promotes its terminal differentiation toward post-mitotic neurons. Our study demonstrates the activity-dependent neuronal differentiation of type-2 progenitor cells by means of excitatory GABAergic inputs.

  • Research Products

    (11 results)

All 2006 2005

All Journal Article (10 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Downregulation of alpha5betal integrin expession during neuronal differentiation in neural stem cells2006

    • Author(s)
      Muramatsu D;Yoshida N;Hishiyama S;Miyamoto Y;Hisatsune T
    • Journal Title

      Animal Cell Technology:Basic&Applied Aspects 14

      Pages: 277-283

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increased number of new neurons in the olfactory bulband hippocampus of adult non-human primates afterocal ischemia2006

    • Author(s)
      Koketsu D;Furuichi Y;Maeda M;Matsuoka N;Miyamoto Y;Hisatsune T
    • Journal Title

      Experimental Neurology 199

      Pages: 92-102

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Downregulation of alpha5betal integrin expession duriiig neuropal differentiation in neural stm cells2006

    • Author(s)
      Muramatu D ; Yoshida N ; Hishiyama S ; Miyamoto Y ; Hisatsune T
    • Journal Title

      Animal Cell Technology : Basic & Applied Aspects 14

      Pages: 277-283

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Increased number of new neurons in the olfactory bulb and hippocampus of adult non-human primates after focal ischemia2006

    • Author(s)
      Koketsu D ; FuruichiY ; Maeda M ; Matsuoka N ; Miyamoto Y ; Hisatsune T
    • Journal Title

      Experimental Neurology 199

      Pages: 92-102

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] GABAergic excitation promotes neuronal differentiation in adult hippocampal progenitor cells2005

    • Author(s)
      Tozuka Y;Fukuda S;Namba T;Seki T;Hisatsune T
    • Journal Title

      Neuron 47

      Pages: 803-815

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Layer-specific production of nitric oxide during corticalcircuit formation in postnatal mouse brain2005

    • Author(s)
      Imura T; Kanatani S;Fukuda S;Miyamoto Y;Hisatsune T
    • Journal Title

      Cerebral Cortex 15

      Pages: 332-340

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transplantation of GABAergic neurons into adult mouseneocortex2005

    • Author(s)
      Muramatsu D;Sato Y;Hishiyama S;Miyamoto Y;Hisatsune T
    • Journal Title

      Experimental Neurology 194

      Pages: 1-11

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] GABAergic excitation promotes neuronal differentiation in adult hippocampal progenitor_cells2005

    • Author(s)
      Tozuka Y ; Fukuda S ; Namba T ; Seki T ; His atsune T
    • Journal Title

      Neuron 47

      Pages: 803-815

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Layer-specific production of nitric oxide during cortical circuit formation in postnatal mouse brain2005

    • Author(s)
      Imura T ; Kanatani S ; Fukuda S ; Miyamoto Y Hisatsune T
    • Journal Title

      Cerebral Cortex 15

      Pages: 332-340

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transplantation of GABAergic neurons into adult mouse neocortex2005

    • Author(s)
      Muramatsu D ; Sato Y ; Hishiyama S ; Miyamoto Y ; Hisatsune T
    • Journal Title

      Experimental Neurology 194

      Pages: 1-11

    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] 哺乳動物的海馬におけるニューロン新生を促進する組成物2005

    • Inventor(s)
      久恒 辰博
    • Industrial Property Number
      特願2005-356712
    • Filing Date
      2005-12-09
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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