2007 Fiscal Year Final Research Report Summary
DISTINCTION OF THE MECHANISMS FOR EXOCYTOSIS BY SIMULTANEOUS MEASUREMENTS WITH EVANESCENCE METHOD AND CONFOCAL METHOD
| Project/Area Number |
17390055
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
TERAKAWA Susumu HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE, PHOTON MEDICAL RESEARCH CENTER, PROFESSOR (50014246)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Takashi HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE, PHOTON MEDICAL RESEARCH CENTER, RESEARCH ASSOCIATE (50283362)
WAKAZONO Yoshihiko HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE, PHOTON MEDICAL RESEARCH CENTER, INVESTIGATOR (90377755)
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| Project Period (FY) |
2005 – 2007
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| Keywords | MIN-6 CELL / SECRETORY GRANULE / INSULIN RELEASE / CRITICAL ANGLE / ULTRA HIGH NA LENS / SLIDING MOVEMENT / INTRACELLULAR PH / EXOCYOTOSIS |
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| Research Abstract |
By devising an optics with the galvano-scanner that provides a capability of fluorescence illumination to a deeper part of cells than the evanescence microscopy, we observed secretory vesicles of 3 different types of cells with a special attention to the movement of the vesicles before exocytosis. The penetration depths of the evanescence illuminations were measured from the shift of the bright spot due to overlaying of a coverslip to the standard coverslip. In adrenaline secreting cells, a change in extracellular pH was not affecting to the flash pattern of the exocytosis, indicating that the rise in pH inside vesicles is not cause for the flash response. In INS-1 cells, expressing GFP-insulin, some vesicles translocated rapidly to the cell membrane before exocytotic flash response. We found such a translocation occurred toward the cell membrane as depth change. In these cells, it was rare to see translocations immediately followed by exocytosis. In MIN-6 cells, many vesicles glided for long distances in parallel to the cell membrane. These vesicles rarely underwent the exocytotic flash response. From these observations, we concluded that the flash response indicates the diffusion of cargo materials from vesicles. A further development of microscopic optics that can measure the velocity of object in short time would be necessary, and we are currently working along this line.
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[Journal Article] Journal of Microscopy2009
Author(s)
Moriguchi C, Egami C, Kawata Y, Terakawa S, Tsuchimori M, Watamane O
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Journal Title
Stroboscopic near-field imaging for the analysis of contraction of muscle cells. 233
Pages: 269-274
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Presentation] 顕微鏡の未来2007
Author(s)
寺川進
Organizer
日本顕微鏡学会関西支部学会
Place of Presentation
岡崎
Year and Date
2007-12-25
Description
「研究成果報告書概要(和文)」より
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