2007 Fiscal Year Final Research Report Summary
Investigation of aldosterone action on ENaC in salt-sensitive hypertension
| Project/Area Number |
17390057
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MARUNAKA Yoshinori Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Professor (00127036)
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| Co-Investigator(Kenkyū-buntansha) |
NIISATO Naomi Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Associate Professor (00237645)
MIYAZAKI Hiroaki Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Associate Professor (30360027)
NAKAJIMA Ken-ichi Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Assistant Professor (40398392)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Epithelial Na Channel / Aldosterone / Hypertension / Renal tubules / Collecting Duct / Intracellular Trafficking / Osmolarity / SGK1 |
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| Research Abstract |
The present study has clarified the following points.
In the salt-sensitive hypertensive Dahl rat, ENaC expression was abnormally increased in high salt diet, but not in the salt-resistant hypertensive Dahl rat.
In the salt-sensitive hypertensive Dahl rat, high salt diet abnormally upregulated the expression of SGK1, which regulates the intracellular trafficking of ENaC.
These observations suggest that in the salt-sensitive hypertensive Dahl rat, expression of ENaC and its regulator, SGK1, is abnormally regulated by high salt-diet, resulting in hypertension.
This abnormally upregulation of ENaC expression was partially diminished by a flavonoid, quercetin. Hyposomotic stress elevated the expression of ENaC.
This hyposmotic stress-induced elevation of ENaC expression was mediated via a decrease in the cytosolic chloride ion concentration.
Aldosterone inhibited the endocytosis of ENaC protein.
The aldosterone-induced ENaC proteins were mainly located in ER
p38 was involved in hyposmotic stress-induced upregulation of ENaC expression.
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Research Products
(51 results)
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[Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expressi on of p21 through a p53-independent pathway in human gastric cancer cells2008
Author(s)
Miyazaki H, Shiozaki A, Niisato N, Ohsawa R, Itoi H, Ueda Y, Otsuji E, Yamagishi H, Iwasaki Y, Nakano T, Nakahari T, Marunaka Y.
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Journal Title
Biochem Biophys Res Commun 366(2)
Pages: 506-12
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells2008
Author(s)
Miyazaki, H., Shiozaki, A., Niisato, N., Ohsawa, R., Itoi, H., Ueda, Y., Otsuji, E., Yamagishi, H., Iwasaki, Y., Nakano, T., Nakahari, T., MARUNAKA, Y
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Journal Title
Biochem Biophys Res Commun 366
Pages: 506-512
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] HCO_3-dependent pH_i recovery and overacidification induced by NH_4^+ pulse in rat lung alveolar type II cells:HCO_3-dependent NH_3 excretion from lungs2007
Author(s)
Tokuda S, Shimamoto C, Yoshida H, Murao H, Kishima G, Ito S, Kubota T, Hanafusa T, Sugimoto T, Niisato N, Marunaka Y, Nakahari T.
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Journal Title
PflugersArch 455(2)
Pages: 223-239
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] An inhaled inducible nitric oxide synthase inhibitor reduces damage of Candida-induced acute lung injury2007
Author(s)
Ohsugi S, Iwasaki Y, Takemura Y, Nagata K, Harada H, Yokomura I, Hosogi S, Yuba T, Niisato N, Miyazaki H, Matsubara H, Fushiki S, Marunaka Y.
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Journal Title
Biomed Res 28(2)
Pages: 91-99
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Epithelial Na^+ channel and ion transport in human nasal polyp and paranasal sinus mucosa2007
Author(s)
Yasuda, M., Niisato, N., Miyazaki, H., Iwasaki, Y., Hama, T., Dejima, K., Hisa, Y., MARUNAKA, Y
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Journal Title
Biochem Biophys Res Commun 362
Pages: 753-758
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Chloride-dependent acceleration of cell cycle via modulation of Rb and cdc2 in osteoblastic cells2007
Author(s)
Maki, M., Miyazaki, H., Nakajima, K., Yamane, J., Niisato, N., Morihara, T., Kubo, T., MARUNAKA, Y
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Journal Title
Biochem Biophys Res Commun 361
Pages: 1038-1043
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] HCO_3 dependent pHi recovery and overacidification induced by NH_4^+ pulse in rat lung alveolar type II cells: HCO_3 dependent NH_3 excretion from lungs2007
Author(s)
Tokuda, S., Shimamoto, C., Yoshida, H., Murao, H., Kishima, G., Ito, S., Kubota, T., Hanafusa, T., Sugimoto, T., Niisato, N., MARUNAKA, Y., Nakahari, T
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Journal Title
Pfluegers Arch (Eur J Physiol) 455
Pages: 223-239
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An inhaled inducible nitric oxide synthase inhibitor reduces damage of Candida-induced acute lung injury2007
Author(s)
Ohsugi, S., Iwasaki, Y., Takemura, Y., Nagata, K., Harada, H., Yokomura, I., Hosogi, S., Yuba, T., Niisato, N., Miyazaki, H., Matsubara, H., Fushiki, S., MARUNAKA, Y
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Journal Title
Biomed Res 28
Pages: 91-99
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Abnormal expression of ENaC and SGK1 mRNA induced by dietary sodium in Dahl salt-sensitively hypertensive rats2007
Author(s)
Aoi, W., Niisato, N., Sawabe, Y., Miyazaki, H., Tokuda, S., Nishio, K., Yoshikawa, T., MARUNAKA, Y
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Journal Title
Cell Biol hat 31
Pages: 1288-1291
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Action of neltenexine on anion secretion in human airway epithelia2007
Author(s)
Niisato, N., Hasegawa, I., Tokuda, S., Taruno, A., Nakajima, K., Miyazaki, H., Iwasaki, Y., MARUNAKA, Y
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Journal Title
Biochem Biophys Res Commun 356
Pages: 1050-1055
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Epithelial ion transport of human nasal polyp and paranasal sinus mucosa2007
Author(s)
Yasuda, M., Niisato, N., Miyazaki, H., Hama, T., Dejima, K., Hisa, Y., MARUNAKA, Y
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Journal Title
Am J Respir Cell Mol Biol 36
Pages: 466-472
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Furosemide,a blocker of Na^+/K^+/2Cl^- cotransporter,diminishes proliferationof poorly differentiated human gastric cancer cells by affecting G_0/G_1 state2006
Author(s)
Shiozaki A, Miyazaki H, Niisato N, Nakahari T, Iwasaki Y, Itoi H, Ueda Y, Yamagishi H, Marunaka Y.
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Journal Title
Journal of Physilogical Sciences 56
Pages: 401-406
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] cGMP modulation of ACh-stimulated exocytosis in guinea pig antral mucous cells2006
Author(s)
Saad, AH., Shimamoto, C., Nakahari, T., Fujiwara, S., Katsu, K., MARUNAKA, Y
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Journal Title
Am J Physiol Gastrointest Liver Physiol 290
Pages: G1138-G1148
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Furosemide, a blocker of Na^+/K^+/2Cl cotransporter, diminishes proliferation of poorly differentiated human gastric cancer cells by affecting G0/G1 state2006
Author(s)
Shiozaki, A., Miyazaki, H., Niisato, N., Nakahari, T., Iwasaki, Y., Itoi, H., Ueda, Y., Yamagishi, H., MARUNAKA, Y
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Journal Title
J Physiol Sci 56
Pages: 401-406
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Synergic action of insulin and genistein on Na^+/K^+/2Cl cotransporter in renal epithelium2005
Author(s)
Ueda-Nishimura, T., Niisato, N., Miyazaki, H., Naito, Y., Yoshida, N., Yoshikawa, T., Nishino, H., MARUNAKA, Y
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Journal Title
Biochem Biophys Res Commun 332
Pages: 1042-1052
Description
「研究成果報告書概要(欧文)」より
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