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2007 Fiscal Year Final Research Report Summary

Investigation of aldosterone action on ENaC in salt-sensitive hypertension

Research Project

Project/Area Number 17390057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

MARUNAKA Yoshinori  Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Professor (00127036)

Co-Investigator(Kenkyū-buntansha) NIISATO Naomi  Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Associate Professor (00237645)
MIYAZAKI Hiroaki  Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Associate Professor (30360027)
NAKAJIMA Ken-ichi  Kyoto Prefectural University of Medicine, Department of Molecular Cell Physiology, Assistant Professor (40398392)
Project Period (FY) 2005 – 2007
KeywordsEpithelial Na Channel / Aldosterone / Hypertension / Renal tubules / Collecting Duct / Intracellular Trafficking / Osmolarity / SGK1
Research Abstract

The present study has clarified the following points.
In the salt-sensitive hypertensive Dahl rat, ENaC expression was abnormally increased in high salt diet, but not in the salt-resistant hypertensive Dahl rat.
In the salt-sensitive hypertensive Dahl rat, high salt diet abnormally upregulated the expression of SGK1, which regulates the intracellular trafficking of ENaC.
These observations suggest that in the salt-sensitive hypertensive Dahl rat, expression of ENaC and its regulator, SGK1, is abnormally regulated by high salt-diet, resulting in hypertension.
This abnormally upregulation of ENaC expression was partially diminished by a flavonoid, quercetin. Hyposomotic stress elevated the expression of ENaC.
This hyposmotic stress-induced elevation of ENaC expression was mediated via a decrease in the cytosolic chloride ion concentration.
Aldosterone inhibited the endocytosis of ENaC protein.
The aldosterone-induced ENaC proteins were mainly located in ER
p38 was involved in hyposmotic stress-induced upregulation of ENaC expression.

  • Research Products

    (51 results)

All 2008 2007 2006 2005

All Journal Article (46 results) (of which Peer Reviewed: 23 results) Presentation (5 results)

  • [Journal Article] Regulation of the paracellular Na+ and Cl-conductances by the NaCI-generated osmotic gradient in a manner dependent on the direction of osmotic gradients2008

    • Author(s)
      Tokuda S, Niisato N, Nakajima K, Manznaka Y.
    • Journal Title

      Biochem Biophys Res Commun 366(2)

      Pages: 464-70

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expressi on of p21 through a p53-independent pathway in human gastric cancer cells2008

    • Author(s)
      Miyazaki H, Shiozaki A, Niisato N, Ohsawa R, Itoi H, Ueda Y, Otsuji E, Yamagishi H, Iwasaki Y, Nakano T, Nakahari T, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 366(2)

      Pages: 506-12

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Intracellular calcium plays a role as the second messenger of hypotonic stress in gene regulation of SGK1 and ENaC in renal epithelial A6 cells2008

    • Author(s)
      Taruno A, Niisato N, Marunaka Y.
    • Journal Title

      Am J Physiol Renal Physiol 294(1)

      Pages: F177-86

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Regulation of the paracellular Na^+ and Cl-conductances by the NaCl-generated osmotic gradient in a manner dependent on the direction of osmotic gradients2008

    • Author(s)
      Tokuda, S., Niisato, N., Nakajima, K., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 366

      Pages: 464-470

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway in human gastric cancer cells2008

    • Author(s)
      Miyazaki, H., Shiozaki, A., Niisato, N., Ohsawa, R., Itoi, H., Ueda, Y., Otsuji, E., Yamagishi, H., Iwasaki, Y., Nakano, T., Nakahari, T., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 366

      Pages: 506-512

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intracellular calcium plays a role as the second messenger of hypotonic stress in gene regulation of SGK1 and ENaC in renal epithelial A6 cells2008

    • Author(s)
      Taruno, A., Niisato, N., MARUNAKA, Y
    • Journal Title

      Am J Physiol Renal Physiol 294

      Pages: 177-286

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Regulation of paracellular ion conductances by NaCl gradients in renal epithelial cells2007

    • Author(s)
      Tokuda S, Niisato N, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 363(3)

      Pages: 566-571

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Epithelial Na+ channel and ion transport in human nasal polyp and paranasal sinus mucosa2007

    • Author(s)
      Yasuda M, Niisato N, Miyazaki H, Iwasaki Y, Hama T, Dejima K, Hisa Y, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 362(3)

      Pages: 753-758

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Chloride-dependent acceleration of cell cycle via modulation of Rb and cdc2 in osteoblastic cells2007

    • Author(s)
      Maki M, Miyazaki H, Nakajima K, Yamane J, Niisato N, Morihara T, Kubo T, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 361(4)

      Pages: 1038-1043

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Aldosterone-induced modification of osmoregulated ENaC trafficking2007

    • Author(s)
      Niisato N, Taruno A, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 361(1)

      Pages: 162-168

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] HCO_3-dependent pH_i recovery and overacidification induced by NH_4^+ pulse in rat lung alveolar type II cells:HCO_3-dependent NH_3 excretion from lungs2007

    • Author(s)
      Tokuda S, Shimamoto C, Yoshida H, Murao H, Kishima G, Ito S, Kubota T, Hanafusa T, Sugimoto T, Niisato N, Marunaka Y, Nakahari T.
    • Journal Title

      PflugersArch 455(2)

      Pages: 223-239

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Essential role of NKCCI in NGF-induced neurite outgrowth2007

    • Author(s)
      Nakajima K, Miyazaki H, Niisato N, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 359(3)

      Pages: 604-610

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] An inhaled inducible nitric oxide synthase inhibitor reduces damage of Candida-induced acute lung injury2007

    • Author(s)
      Ohsugi S, Iwasaki Y, Takemura Y, Nagata K, Harada H, Yokomura I, Hosogi S, Yuba T, Niisato N, Miyazaki H, Matsubara H, Fushiki S, Marunaka Y.
    • Journal Title

      Biomed Res 28(2)

      Pages: 91-99

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Involvement of p38 MAPK in hypotonic stress-induced stimulation of beta-and gamma-ENaC expression in renal epithelium2007

    • Author(s)
      Niisato N, Taruno A, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 358(3)

      Pages: 819-824

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Abnormal expression of ENaC and SGKl mRNA induced by dietary sodium in Dahl salt-sensitively hypertensive rats2007

    • Author(s)
      Aoi W, Niisato N, Sawabe Y, Miyazaki H, Tokuda S, Nishio K, Yoshikawa T, Marunaka Y.
    • Journal Title

      Cell Biol Int. 31(10)

      Pages: 1288-1291

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Action of neltenexine on anion secretion in human airway epithelia2007

    • Author(s)
      Niisato N, Hasegawa I, Tokuda S, Taruno A, Nakajima K, Miyazaki H, Iwasaki Y, Marunaka Y.
    • Journal Title

      Biochem Biophys Res Commun 356(4)

      Pages: 1050-1055

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Hypotonicity stimulates renal epithelial sodium transport by activating JNK viareceptor tyrosine kinases2007

    • Author(s)
      Taruno A, Niisato N, Marunaka Y.
    • Journal Title

      Am J Physiol Renal Physiol 293(1)

      Pages: Fl28-Fl38

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Physiological significance of hypotonicity-induced regulatory volume decrease: reduction in intracellular Cl-concentration acting as an intracellular signaling2007

    • Author(s)
      Miyazaki H, Shiozaki A, Niisato N, Marunaka Y.
    • Journal Title

      Am J Physiol Renal Physiol 292(5)

      Pages: F1411-F1417

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Epithelial ion transport of human nasal polyp and paranasal sinus mucosa2007

    • Author(s)
      Yasuda M, Niisato N, Miyazaki H, Hama T, Dejima K, Hisa Y, Marunaka Y
    • Journal Title

      Am J Respir Cell Mol Biol 36

      Pages: 466-472

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Regulation of paracellular ion conductances by NaC1 gradients in renal epithelial cells2007

    • Author(s)
      Tokuda, S., Niisato, N., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 366

      Pages: 464-470

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Epithelial Na^+ channel and ion transport in human nasal polyp and paranasal sinus mucosa2007

    • Author(s)
      Yasuda, M., Niisato, N., Miyazaki, H., Iwasaki, Y., Hama, T., Dejima, K., Hisa, Y., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 362

      Pages: 753-758

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Chloride-dependent acceleration of cell cycle via modulation of Rb and cdc2 in osteoblastic cells2007

    • Author(s)
      Maki, M., Miyazaki, H., Nakajima, K., Yamane, J., Niisato, N., Morihara, T., Kubo, T., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 361

      Pages: 1038-1043

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Aldosterone-induced modification of osmoregulated ENaC trafficking2007

    • Author(s)
      Niisato, N., Taruno, A., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 361

      Pages: 162-168

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] HCO_3 dependent pHi recovery and overacidification induced by NH_4^+ pulse in rat lung alveolar type II cells: HCO_3 dependent NH_3 excretion from lungs2007

    • Author(s)
      Tokuda, S., Shimamoto, C., Yoshida, H., Murao, H., Kishima, G., Ito, S., Kubota, T., Hanafusa, T., Sugimoto, T., Niisato, N., MARUNAKA, Y., Nakahari, T
    • Journal Title

      Pfluegers Arch (Eur J Physiol) 455

      Pages: 223-239

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Essential role of NKCCl in NGF-induced neurite outgrowth2007

    • Author(s)
      Nakajima, K., Miyazaki, Y., Niisato, N., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 359

      Pages: 604-610

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An inhaled inducible nitric oxide synthase inhibitor reduces damage of Candida-induced acute lung injury2007

    • Author(s)
      Ohsugi, S., Iwasaki, Y., Takemura, Y., Nagata, K., Harada, H., Yokomura, I., Hosogi, S., Yuba, T., Niisato, N., Miyazaki, H., Matsubara, H., Fushiki, S., MARUNAKA, Y
    • Journal Title

      Biomed Res 28

      Pages: 91-99

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Involvement of p38 MAPK in hypotonic stress-induced stimulation of beta- and gamma-ENaC expression in renal epithelium2007

    • Author(s)
      Niisato, N., Taruno, A., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 358

      Pages: 819-824

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Abnormal expression of ENaC and SGK1 mRNA induced by dietary sodium in Dahl salt-sensitively hypertensive rats2007

    • Author(s)
      Aoi, W., Niisato, N., Sawabe, Y., Miyazaki, H., Tokuda, S., Nishio, K., Yoshikawa, T., MARUNAKA, Y
    • Journal Title

      Cell Biol hat 31

      Pages: 1288-1291

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Action of neltenexine on anion secretion in human airway epithelia2007

    • Author(s)
      Niisato, N., Hasegawa, I., Tokuda, S., Taruno, A., Nakajima, K., Miyazaki, H., Iwasaki, Y., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 356

      Pages: 1050-1055

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hypotonicity stimulates renal epithelial sodium transport by activating JNK via receptor tyrosine kinases2007

    • Author(s)
      Taruno, A., Niisato, N., MARUNAKA, Y
    • Journal Title

      Am J Physiol Renal Physiol 293

      Pages: F128-138

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Physiological significance of hypotonicity-induced regulatory volume decrease: reduction in intracellular Cl' concentration acting as an intracellular signaling2007

    • Author(s)
      Miyazaki, H., Shiozaki, A., Niisato, N., MARUNAKA, Y
    • Journal Title

      Am J Physiol Renal Physiol 292

      Pages: F1411-1417

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Epithelial ion transport of human nasal polyp and paranasal sinus mucosa2007

    • Author(s)
      Yasuda, M., Niisato, N., Miyazaki, H., Hama, T., Dejima, K., Hisa, Y., MARUNAKA, Y
    • Journal Title

      Am J Respir Cell Mol Biol 36

      Pages: 466-472

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Aldosterone-induced abnormal regulation of ENaC and SGKl in Dahl salt-sensitive rat2006

    • Author(s)
      Aoi W, Niisato N, Sawabe Y, Miyazaki H, Marunaka Y.
    • Journal Title

      Biochemical and Biophysical Research Communications 341

      Pages: 376-381

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Ambroxol-induced modification of ion transport on human airway Calu-3 Epithelia2006

    • Author(s)
      Hasegawa I, Niisato N, Iwasaki Y, Marunaka Y.
    • Journal Title

      Biochemical and Biophysical Research Communications 343

      Pages: 475-482

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Cyclic GMP modulation of ACh-stimulated exocytosis in guinea pig antral mucous cells.2006

    • Author(s)
      Saad AH, Shimamoto C, Nakahari T, Fujiwara S, Katsu K and Marunaka Y.
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol 290

      Pages: G1138-G1148

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Furosemide,a blocker of Na^+/K^+/2Cl^- cotransporter,diminishes proliferationof poorly differentiated human gastric cancer cells by affecting G_0/G_1 state2006

    • Author(s)
      Shiozaki A, Miyazaki H, Niisato N, Nakahari T, Iwasaki Y, Itoi H, Ueda Y, Yamagishi H, Marunaka Y.
    • Journal Title

      Journal of Physilogical Sciences 56

      Pages: 401-406

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] UT-B1 urea transporter plays a noble role as active water transporter in C6 glial cells2006

    • Author(s)
      Ogami A, Miyazaki H, Niisato N, Sugimoto T, Marunaka Y.
    • Journal Title

      Biochemical and Biophysical Research Communications 351

      Pages: 619-624

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Aldosterone-induced abnormal regulation of ENaC and SGK1 in Dahl salt-sensitive rat2006

    • Author(s)
      Aoi, W., Niisato, N., Sawabe, Y., Miyazaki, H., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 341

      Pages: 376-381

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ambroxol-induced modification of ion transport in human airway Calu-3 epithelia2006

    • Author(s)
      Hasegawa, I., Niisato, N., Iwasaki, Y., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 343

      Pages: 475-482

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] cGMP modulation of ACh-stimulated exocytosis in guinea pig antral mucous cells2006

    • Author(s)
      Saad, AH., Shimamoto, C., Nakahari, T., Fujiwara, S., Katsu, K., MARUNAKA, Y
    • Journal Title

      Am J Physiol Gastrointest Liver Physiol 290

      Pages: G1138-G1148

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Furosemide, a blocker of Na^+/K^+/2Cl cotransporter, diminishes proliferation of poorly differentiated human gastric cancer cells by affecting G0/G1 state2006

    • Author(s)
      Shiozaki, A., Miyazaki, H., Niisato, N., Nakahari, T., Iwasaki, Y., Itoi, H., Ueda, Y., Yamagishi, H., MARUNAKA, Y
    • Journal Title

      J Physiol Sci 56

      Pages: 401-406

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] UT-B1 urea transporter plays a noble role as active water transporter in C6 glial cells2006

    • Author(s)
      Ogami, A., Miyazaki, H., Niisato, N., Sugimoto, T., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 351

      Pages: 619-624

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Synergic action of insulin and genistein on Na^+/K^+2CI^-contransporter in renal epithelium.2005

    • Author(s)
      Ueda-Nishimura T, Niisato N, Miyazaki H, Naito Y, Yoshida N, Yoshikawa T, Nishino H, Marunaka Y.
    • Journal Title

      Biochemical and Biophysical Research Communications 332

      Pages: 1042-1052

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Quercetin and NPPB-induced diminution of aldosterone action on Na^+ absorptlon and ENaC expression in renal epithelium2005

    • Author(s)
      Fujimoto S, Niisato N, Sugimoto T, Marunaka Y.
    • Journal Title

      Biochemical and Biophysical Research Communications 336

      Pages: 401-407

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Synergic action of insulin and genistein on Na^+/K^+/2Cl cotransporter in renal epithelium2005

    • Author(s)
      Ueda-Nishimura, T., Niisato, N., Miyazaki, H., Naito, Y., Yoshida, N., Yoshikawa, T., Nishino, H., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 332

      Pages: 1042-1052

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Quercetin and NPPB-induced diminution of aldosterone action on Na^+ absorption and ENaC expression in renal epithelium2005

    • Author(s)
      Fujimoto, S., Niisato, N., Sugimoto, T., MARUNAKA, Y
    • Journal Title

      Biochem Biophys Res Commun 336

      Pages: 401-407

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Intracellular chloride-dependent modulation of aldosterone action on Na^+ reabsorption2007

    • Author(s)
      Niisato N, Manxnaka Y.
    • Organizer
      Aldosterone and ENaC:from Gene to Disease
    • Place of Presentation
      Zemmatt,Switzerland
    • Year and Date
      20071003-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Regulation ofENaC function by hypotonic stress in renal epithelial A6 cells: genomic and non-genomic mechanisms2007

    • Author(s)
      Taruno A, Niisato N, Marunaka Y.
    • Organizer
      Aldosterone and ENaC:from Gene to Disease
    • Place of Presentation
      Switerland
    • Year and Date
      20071003-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cytosolic Cl^- acts as a signal molecule in hypotonic stress by regulating tyrosine phosphorylation state of src kinase2007

    • Author(s)
      Niisato, Marunaka Y.
    • Organizer
      The 84th Annual Meeting of The Physiological Society of Japan
    • Place of Presentation
      Osaka
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Cl^-dependent regulatory mechanism of proliferation of human gastric cancer cells via G0/G1 arrest2007

    • Author(s)
      Miyazaki H, Shiozaki A, Niisato N, Marunaka Y.
    • Organizer
      The 84th Annual Meeting of The Physiological Society of Japan
    • Place of Presentation
      Osaka
    • Year and Date
      20070320-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] フラボノイドによる血圧制御の分子機構2007

    • Author(s)
      丸中 良典、新里 直美、青井 渉、宮崎 裕明、中島 謙一、徳田 深作
    • Organizer
      日本農芸化学会2007年度大会
    • Place of Presentation
      東京
    • Year and Date
      2007-03-24
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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