2006 Fiscal Year Final Research Report Summary
Molecular functions of HLH protein Id2 in controlling cell differentiation and proliferation
| Project/Area Number |
17390092
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | University of Fukui |
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Principal Investigator |
YOKOTA Yoshifumi University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (50222386)
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| Co-Investigator(Kenkyū-buntansha) |
KUROOKA Hisanori University of Fukui, Faculty of Medical Sciences, Associate Professor, 医学部, 助教授 (00293879)
KARAYA Kazuhiro University of Fukui, Faculty of Medical Sciences, Assistant Professor, 医学部, 助手 (70233997)
MORI Kentaro University of Fukui, Faculty of Medical Sciences, COE Researcher, 医学部, COE研究員 (50397296)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Id2 / cell differentiation / growth control / immediate early gene / development |
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| Research Abstract |
1. In growth-arrested NIH3T3 fibroblast cells, Id2 gene expression was induced by serum even in the presence of cycloheximide, a protein synthesis inhibitor, demonstrating Id2 to be an immediate early gene. The response was mediated by a transcription factor RFX1 that bound the element located 3.0 kb upstream of Id2. Interestingly, RFX1 was also involved in the suppression of Id2 gene expression in growth-arrested cells.
2. Id1 has a nuclear export signal in its Oterminal end of the HLH region, unlike Id2. We found that Arg96 of the signal is essential for subcellular localization of Id1.
3. We failed to identify Id2-interacting molecules that exhibited potential relevant functions.
4. We found that Id2 plays an important role in controlling differentiation of intestinal epithelial cells.
5. Several functions of Id2 in osteoclasts, ternary lymphoid organ development and so on were revealed by analyses of Id2-deficient mice.
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