2006 Fiscal Year Final Research Report Summary
Comprehensive understanding of the virulence mechanisms of Entamoeba histolytica
Project/Area Number |
17390124
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Parasitology (including Sanitary zoology)
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Research Institution | Gunma University |
Principal Investigator |
NOZAKI Tomoyoshi GUNMA UNIVERSITY, Department of Parasitology, Professor, 大学院・医学系研究科, 教授 (60198588)
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Co-Investigator(Kenkyū-buntansha) |
SAITO Yumiko (NAKANO Yumiko) National Institute of Infectious Diseases, Department of Parasitology, Senior Research Scientist, 寄生動物部, 主任研究官 (30321764)
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Project Period (FY) |
2005 – 2006
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Keywords | Entamoeba histolytica / Pathogenesis / Cysteine protease / Transcriptome / Vesicular trafficking / Infectious diseases / Parasite |
Research Abstract |
For comprehensive understanding of pathogenesis of Entamoeba histolytica, transcriptome analysis using DNA microarrays was conducted. The array covers 7712 genes out of 9435 genes in the TIGR genome database. Approximately 80% of genes were expressed in the reference strain axenically cultivated. We also established highly-virulent or attenuated lines by passaging the hamster liver or continuously cultivating in the axenic medium. These lines showed remarkable differences in both in vitro and in vivo pathogenicity. Transcriptome analysis of these lines revealed approximately 200 genes were differentially expressed, about 30 or 170 of which were up- or down-regulated in the highly-virulent line. We also discovered a new family of intrinsic inhibitor, named ICP, which regulated transport and activity of the major virulence factor cysteine proteases (CP). E. histolytica possesses ICP1 and ICP2, located in the cytosol and lysosomes/phagosomes, respectively. Both ICP1 and ICP2 bound to the major CPs (CP1,2,5)) in vivo, and inhibited these CPs in vitro. CP existed 1000-fold in excess to ICP. The amoeba transformants overexpressing ICP1 or ICP2 showed 60-90% reduction of both intracellular and secreted CP activity. These data indicate that ICPs bind to and regulate CP by binding to CPs in distinct compartments and further leading to degradation.
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Research Products
(18 results)
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[Journal Article] Kinetics and strain variation of phagosome proteins of Entamoeba histolytica by proteomic analysis.2006
Author(s)
Okada, M., Huston, C.D., Oue, M., Mann, B.J., Petri, Jr., W.A., Kita, K., Nozaki, T.
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Journal Title
Mol. Biochem. Parasitol. 145
Pages: 171-183
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Two cysteine protease inhibitors, EhICP1 and 2, localized in distinct compartments, negatively regulate secretion in Entamoeba histolytica.2006
Author(s)
Sato, D., Nakada-Tsukui, K., Okada, M., Nozaki, T.
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Journal Title
FEBS Lett. 580
Pages: 5306-5312
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome.2006
Author(s)
Gilchrist, C.A., Houpt, E., Trapaidze, N., Fei, Z., Crasta, O., Asgharpour, A., Evans, C., Martino-Catt, S., Baba, D.J., Stroup, S., Hamano, S., Ehrenkaufer, G., Okada, M., Singh, U., Nozaki T., Mann, B.J., Petri, Jr., W.
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Journal Title
Mol. Biochem. Parasitol. 147
Pages: 163-176
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Kinetics and strain variation of phagosome proteins of Entamoeba histolytica by proteomic analysis.2006
Author(s)
Okada, M., Huston, C.D., Oue, M., Mann, B.J., Petri, Jr., W.A., Kita, K., Nozaki, T.
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Journal Title
Mol.Biochem.Parasitol. 145
Pages: 171-183
Description
「研究成果報告書概要(欧文)」より
-
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[Journal Article] Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome.2006
Author(s)
Gilchrist, C.A., Houpt, E., Trapaidze, N., Fei, Z., Crasta, O., Asgharpour, A., Evans, C., Martino-Catt, S., Baba, D.J., Stroup, S., Hamano, S., Ehrenkaufer, G., Okada, M., Singh, U., Nozaki, T., Mann, B.J., Petri, Jr., W.
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Journal Title
Mol.Biochem.Parasitol. 147
Pages: 163-176
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification and gene expression analysis of a large family of transmembrane kinases related to the Gal/GalNAc lectin in Entamoeba histolytica.2005
Author(s)
Beck, D.L., Boettner, D., Dragulev, B., Ready, L., Mackey, A.J., Nozaki, T., Pearson, W.R., Petri, Jr., W.A.
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Journal Title
Eukaryot. Cell 4
Pages: 722-732
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica.2005
Author(s)
Okada, M., Huston, C.D., Mann, B.J., Petri, Jr., W.A., Kita, K., Nozaki, T.
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Journal Title
Eukaryot. Cell 4
Pages: 827-831
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Identification and gene expression analysis of a large family of transmembrane kinases related to the Gal/GaINAc lectin in Entamoeba histolytica2005
Author(s)
Beck, D.L., Boettner, D., Dragulev, B., Ready, L., Mackey, A.J., Nozaki, T., Pearson, W.R., Petri, Jr., W.A.
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Journal Title
Eukaryot.Cell 4
Pages: 722-732
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Proteomic analysis of phagocytosis in the enteric protozoan parasite Entamoeba histolytica.2005
Author(s)
Okada, M., Huston, C.D., Mann, B.J., Petri, Jr., W.A., Kita, K., Nozaki, T.
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Journal Title
Eukaryot.Cell 4
Pages: 827-831
Description
「研究成果報告書概要(欧文)」より
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