Molecular biological analysis of age-dependently expressed genes : application to age estimation in legal medicine

2008 Fiscal Year Final Research Report

• Project/Area Number: 17390199
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: University of Fukui
• Principal Investigator: IIDA Reiko University of Fukui, 医学部, 助教 (40139788)
• Co-Investigator(Kenkyū-buntansha): MATSUKI Matsuki 福井大学, 医学部, 教授 (10126617)
• Project Period (FY): 2005 – 2008
• Keywords: 年齢推定 / 老化 / 遺伝子発現

Research Abstract

年齢依存性発現生体分子を"マーカー"とした法医学的年齢推定法を開発するための基礎的研究を行い, 以下の成果を得た。(1) マウス肝において年齢依存性発現を示す遺伝子を検索し, 6個の既知遺伝子を同定した。(2) 若年者の尿に特異的に見い出される糖タンパク質Ugl-Yについて解析を行ない, Ugl-Yがfibronectinの一部の配列に完全に一致すること, また, 血清に由来することを明らかにした。(3) マウス腎で見い出された年齢依存性発現生体分子M-LPはヒトにも存在し, 活性酸素の代謝機構に関与していることが示された。

Research Products

• [Journal Article] Multiplex single base extension method for simultaneous genotyping of non-synonymous SNPs in the three human SOD genes (2008)
  • Author(s): R. Iida, E. Tsubota, H. Takeshita, T. Yasuda
  • Journal Title: Electrophoresis 29 Pages: 4788-4794
  • Peer Reviewed
• [Journal Article] Identification of age-dependently expressed genes in mouse liver by differential display-PCR analysis (2008)
  • Author(s): E. Tsubota, T. Yasuda, R. Iida
  • Journal Title: Comparative Biochem. Physiol D Proteomics 3 Pages: 91-95
  • Peer Reviewed
• [Journal Article] Identification of novel fibronectin fragments detected specifically in juvenile urine (2007)
  • Author(s): R. Iida, T. Yasuda, K. Kishi
  • Journal Title: FEBS J 274 Pages: 3939-3947
  • Peer Reviewed
• [Journal Article] Hypoxia induces up- regulation of the deoxyribonuclease I gene in the human pancreatic cancer cell line QGP-1. Biochim (2007)
  • Author(s): Y. Kominato, R. Iida, T. Nakajima,Y. Tajima, R. Takagi, C. Makita, K. Kishi, M. Ueki, Y. Kawai, T. Yasuda
  • Journal Title: Biophys. Acta 1770 Pages: 1567-1575
  • Peer Reviewed
• [Journal Article] Susceptibility of mammalian deoxyribonucleases I(DNases I)to proteolysis by proteases and its relationships to tissue distribution : biochemical and molecular analysis of equine DNase I (2007)
  • Author(s): M. Ueki, H. Takeshita, J. Fujihara, G. Ueta, T. Nakajima, Y. Kominato, K. Kishi, R. Iida, T. Yasuda
  • Journal Title: Comparative Biochem. Physiol. B 148 Pages: 93-102
  • Peer Reviewed
• [Journal Article] Characterization of human deoxyribonuclease I gene(DNASE1)promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation (2006)
  • Author(s): K. Kominato, M. Ueki, R. Iida, Y. Kawai, T. Nakajima, C. Makita, M. Itoi, Y. Tajima, K. Kishi, T. Yasuda
  • Journal Title: FEBS J 273 Pages: 3094-105
  • Peer Reviewed
• [Journal Article] Human Mpv17-like protein is localized in peroxisomes and regulate expression of antioxidant enzymes (2006)
  • Author(s): R. Iida, T. Yasuda, E. Tsubota, H. Takatsuka, T. Matsuki, K. Kishi
  • Journal Title: Biochem. Biophys. Res. Commun 344 Pages: 948-54
  • Peer Reviewed
• [Journal Article] Quantitative change in mitochondrial DNA content in various mouse tissues during aging (2005)
  • Author(s): M. Masuyama, R. Iida, H. Takatsuka, T. Yasuda, T. Matsuki
  • Journal Title: Biochim. Biophys. Acta 1723 Pages: 302-308
  • Peer Reviewed
• [Journal Article] A novel alternative spliced Mpv17-like protein isoform localizes in cytosol and is expressed in a kidney-specific and adult-specific manner (2005)
  • Author(s): R. Iida, T. Yasuda, E. Tsubota, H. Takatsuka, M. Masuyama, T. Matsuki, K. Kishi
  • Journal Title: Exp. Cell Res 302 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Simultaneous genotyping of eleven non-synonymous SNPs in the four glutathione peroxidase genes using the multiplex single base extension method
  • Author(s): R. Iida, E. Tsubota, I. Yuasa, H. Takeshita, T. Yasuda
  • Journal Title: Clin. Chim. Acta (in press)
  • Peer Reviewed
• [Presentation] Identification of novel Fibronec in fragments detected specifically in Juvenile urine (2008)
  • Author(s): 飯田礼子, 安田年博
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 核酸分解酵素DNASEI遺伝子の転写調節機構の解明 (2008)
  • Author(s): 小湊慶彦, 中島たみ子, 田島裕, 高木利恵, 竹下治男, 藤原純子, 飯田礼子, 安田年博
  • Organizer: 第92次日本法医学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-24
• [Presentation] マウスの年齢依存性発現分子M-LPのヒトホモログの遺伝子構造 (2008)
  • Author(s): 飯田礼子, 松木孝澄, 安田年博
  • Organizer: 第92次日本法医学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-24
• [Presentation] Identification and characterizaion of a human homolog of the M-LP(M-LPH) gene (2007)
  • Author(s): 飯田礼子, 安田年博
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Presentation] マウス腎の年齢依存性発現分子M-LPのヒトホモログの同定 (2007)
  • Author(s): 飯田礼子, 坪田悦子, 松木孝澄, 安田年博
  • Organizer: 日本DNA多型学会第16回学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-11-15
• [Presentation] マウス腎新規タンパク質M-LPの細胞局在型イソフォームの同定 (2007)
  • Author(s): 飯田礼子, 坪田悦子, 松木孝澄, 安田年博
  • Organizer: 第91次日本法医学会総会
  • Place of Presentation: 秋田
  • Year and Date: 2007-05-18
• [Presentation] Age-dependently expressed genes in mouse tis ues revealed by the fluorescence differential display-PCR technique (2006)
  • Author(s): R. Iida, T. Yasuda, T. Matsuki, E. Tsubota
  • Organizer: European Conference of Aging 2006
  • Place of Presentation: Innsbruck, Austria
  • Year and Date: 2006-11-29
• [Presentation] A cytosolic isofom of Mpv17-like protein is expressed in an adult-specific manner (2006)
  • Author(s): R. Iida, T. Matsuki, T. Yasuda
  • Organizer: 20th IUBMB International Congress of Biochemisry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-21
• [Presentation] ヒト尿中に検出される若年者特異的タンパク質(Ugl-Y)の化学構造 (2006)
  • Author(s): 飯田礼子, 安田年博, 岸紘一郎
  • Organizer: 第90次日本法医学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2006-04-28
• [Presentation] マウスの年齢依存性発現分子, M-LP, の細胞質局在型イソフォーム (2005)
  • Author(s): 飯田礼子, 高塚和, 坪田悦子, 松木孝澄, 安田年博, 岸紘一郎
  • Organizer: 日本DNA多型学会第14回学術集会
  • Place of Presentation: 前橋
  • Year and Date: 2005-11-24
• [Book] マウス腎の年齢依存性発現分子M-LPのヒトホモログの同定DNA多型第16巻 (2008)
  • Author(s): 飯田礼子, 坪田悦子, 松木孝澄, 安田年博
  • Total Pages: 213-216
  • Publisher: 東洋書店
• [Book] マウスの年齢依存性発現分子, MLP, の細胞質局在型イソフォーム. DNA多型第14巻 (2006)
  • Author(s): 飯田礼子, 坪田悦子, 高塚尚和, 松木孝澄, 安田年博, 岸紘一郎
  • Total Pages: 160-163
  • Publisher: 東洋書店
• [Book] ヒトDNaseIの分子多様性と心疾患. DNA多型第14巻 (2006)
  • Author(s): 安田年博, 植木美鈴, 飯田礼子, 河合康幸, 小湊慶彦, 中島たみ子, 岸紘一郎
  • Total Pages: 11-17
  • Publisher: 東洋書店
• [Remarks] 新規塩基配列のGenBankへの登録M-LPs cDNA : AY513273, M-LPH1 : DQ004255, M-LPH2 : DQ004256, mouse liver novel EST : ES452733, ES452734