2006 Fiscal Year Final Research Report Summary
Production of sporadic ALS model mice by targeting the ADAR2 gene in motor neurons
| Project/Area Number |
17390251
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KWAK Shin The University of Tokyo, Faculty off Medicine, Department of Neurology, Associate Professor (40160981)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Ryosuke University of Kyoto, Department of Neurology, Professor (90216771)
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| Project Period (FY) |
2005 – 2006
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| Keywords | Degenerative neurological disease / Amyotrophic Lateral Sclerosis / glutamate receptor / AMPA receptor / RNA editing / GluR2 / ADAR2 / knockout mouse |
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| Research Abstract |
We recently demonstrated that Q/R site-RNA editing of GluR2, a subunit of AMPA receptors, is markedly decreased in motor neurons of sporadic, but not familial, ALS patients in a disease-specific and neuronal class-selective manner. GluR2 Q/R. site-editing is specifically catalyzed by adenosine deaminase acting on RNA type 2 (ADAR2) and systemic ADAR2-null mice die young from status epilepticus due to a marked reduction in RNA editing at this site. Reduction of this enzyme activity is likely the cause of GluR2 Q/R site-underediting and neuronal death observed in sporadic ALS motor neurons.
To investigate whether deficient ADAR2 is a cause of slow death of motor neurons as seen in sporadic ALS, we developed genetically modified mice in that the ADAR2 gene is selectively knocked out in motor neurons using Cre-loxP system. We investigated the changes in motor function, survival length, neuronal loss of ADAR2-deficient neurons in these mice as compared to control mice.
These mutant mice displayed significantly shorter mean survival than control mice and progressive motor-selective behavioral deficit in a Cre expression-dependent manner. Motor neurons deficient for ADAR2 underwent slowly progressive death, whereas ADAR2-expressing motor neurons remains intact during the mouse life. RNA editing at the GluR2 Q/R site was completely lost in dying, ADAR2-deficient motor neurons.
Here we demonstrated that loss of ADAR2 induces GluR2 Q/R site-underediting with resultant slowly progressive death of motor neurons in the ADAR2 knockout mice. Present results likely indicate that ADAR2 under-activity may be a cause of neuronal death in sporadic ALS. This mutant mouse model would be useful in research into sporadic ALS.
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[Journal Article] Quantitative evaluation of the pyramidal tract segmented by diffusion tensor tractography : feasibility study in patients with ainyotrophic lateral sclerosis.2005
Author(s)
Aoki S, Iwata NK, Masutani Y. Yoshida M, Abe 0, Ugawa Y, Masumoto T, Mori H, Hayashi N, Kabasawa H, Kwak S, Takahashi S, Tsuji S, Ohtomo K
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Journal Title
Radiat Med 23
Pages: 195-9
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] ALSの分子生物学2005
Author(s)
日出山拓人, 河原行郎, 郭 伸
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Journal Title
Current Insights in Neurological Science 13
Pages: 2-3
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Quantitative evaluation of the pyramidal tract segmented by diffusion tensor tractography: feasibility study in patients with amyotrophic lateral sclerosis.2005
Author(s)
Aoki S, Iwata NK, Masutani Y, Yoshida M, Abe 0, Ugawa Y, Masumoto T, Mori H, Hayashi N, Kabasawa H, Kwak S, Takahashi S, Tsuji S, Ohtomo K
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Journal Title
Radiat Med 23
Pages: 195-9
Description
「研究成果報告書概要(欧文)」より
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[Presentation] RNA編集異常による孤発性ALSモデルマウスの開発と検討2007
Author(s)
郭 伸, 日出山拓人, 西本祥仁, 山下雄也, 辻省次, 高橋良輔, 三澤日出巳, 鈴木岳之
Organizer
Neuro2007 第30回日本神経科学大会・第50回日本神経化学会大会、第17回日本神経回路学会大会
Place of Presentation
横浜
Year and Date
20070910-20070912
Description
「研究成果報告書概要(和文)」より
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[Presentation] 孤発性筋萎縮性側索硬化症のRNA編集異常2007
Author(s)
日出山拓人, 西本祥仁, 山下雄也, 伊藤杏子, 辻省次, 高橋良輔, 三澤日出巳, 鈴木岳之, 郭 伸
Organizer
第48回日本神経学会総会
Place of Presentation
名古屋
Year and Date
20070516-20070518
Description
「研究成果報告書概要(和文)」より
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[Presentation] 孤発性ALSのバイオマーカーの開発2007
Author(s)
西本祥仁, 日出山拓人, 山下雄也, 鈴木則宏, 郭 伸
Organizer
第48回日本神経学会総会
Place of Presentation
名古屋
Year and Date
20070516-20070518
Description
「研究成果報告書概要(和文)」より
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[Presentation] 初代培養脊髄運動ニューロンにおける興奮性アミノ酸毒性の検討2005
Author(s)
深田 潤, 相澤仁志, 牧田圭弘, 鈴木康博, 油川陽子, 片山隆行, 榎本(中谷)雪, 菊池健次郎, 木村 隆, 箭原 修, 濤川一彦, 木山博資, 郭 伸
Organizer
第46回日本神経学会総会
Place of Presentation
鹿児島
Year and Date
20050525-20050527
Description
「研究成果報告書概要(和文)」より
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