2006 Fiscal Year Final Research Report Summary
Analysis of the role of angiogenesis and non-parenchymal cells during hepatic regeneration.
| Project/Area Number |
17390375
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
FUJIMOTO Jiro Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (90199373)
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| Co-Investigator(Kenkyū-buntansha) |
IIMURO Yuji Hyogo College of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (30252018)
YAMANAKA Juniti Hyogo College of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90289083)
HIRANO Tadamiti Hyogo College of Medicine, Faculty of Medicine, Research Associate, 医学部, 助手 (90340968)
OKADA Toshihiro Hyogo College of Medicine, Faculty of Medicine, Research Associate, 医学部, 助手 (70351799)
NAKANISHI Kenji Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (60172350)
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| Project Period (FY) |
2005 – 2006
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| Keywords | angiogenesis / HGF / NK4 / VEGF / sFlt-1 / liver regeneration |
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| Research Abstract |
We investigated the influence of anti-angiogenesis for hepatic regeneration using HGF antagonist (NK4) and suppressor of VEGF receptor (sFlt-1). NK4 expressing adenoviral vector (Ad.NK4) or sFlt-1 expressing adenoviral vector (Ad.sFlt-1) were infected mice liver. Seventy percent hepatectomy was performed 48 hours after infection. LacZ expressing adenoviral vector (Ad.LacZ) was used as control. Expression of Nk4 or sFlt-1 reached maximum level 48 hours after infection (NK4: 300ng/ml, sFlt-1:350ng/ml) and sustained for 2 weeks. Remnant liver was removed at 12, 24, 36, 48, 72 and 120 hrs after partial hepatectomy, and hepatic regeneration was assessed. As a result, increase of remnant hepatic weight was significantly delayed in Ad.NK4 or Ad.sFlt-1 infected mice. Results of mitotic cells and BrdU index showed significant delay of hepatic regeneration in Ad.NK4 or Ad.sFlt-1 infected mice at 48 or 36 hours after hepatectomy. Immnuhistochemical staining of CD31 showed suppression of sinusoidal endothelial cells growth in Ad.NK4 infected mice. Ang-1, Ang-2, Tie-1, and Tie-2 are considered to be crucial in the process of maturation of vessels. Real time PCR showed suppression of mRNA of Ang-1, Ang-2, Tie-1, and Tie-2 with significant difference. These results suggested that hepatic regeneration after hepatectomy closely correlates with angiogenesis.
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[Journal Article] Toll-like receptor signaling in the liver.2006
Author(s)
Seki, E., Brenner, D., Schwabe, R.
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Journal Title
Toll-like receptors in inflammation, ( ONeill, L.A.J. and Brint, E. eds.), Birkhauser Verlag Basel, Switzerland 125-142
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Reactive oxygen species modify oligosaccharides of glycoproteins in vivo : a study of a spontaneous acute hepatitis model rat (LEC rat).2006
Author(s)
Yasuda, J., Eguchi, H., Fujiwara, N., Ookawara, T., Kojima, S., Yamaguchi, Y, Nishimura, M., Fujimoto, J., Suzuki, K.
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Journal Title
Biochem. Biophys. Res. Commun. 342・1
Pages: 127-134
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hepatic effects of influxed endothelin-1 from portal vein : in situ portal vein infusion model using dogs.2006
Author(s)
Kuroda, N., Yamanaka, J., Okada, T., Hirano, T., limuro, Y., Fujimoto, J.
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Journal Title
J. Hepatobiliary Pancreat. Surg. 13・2
Pages: 160-166
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hepatocyte growth factor prevents lupus nephritis in a murine lupus model of chronic graft-versus-host disease.2006
Author(s)
Kuroiwa, T., Iwasaki, T., Imado, T., Sekiguchi, M., Fujimoto, J., Sano, H.
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Journal Title
Arthritis Res. Ther. 8・4
Pages: R123
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The impact of 3-D virtual hepatectomy simulation in living-donor liver transplantation.2006
Author(s)
Yamanaka, J., Saito, S., limuro, Y., Hirano, T., Okada, T., Kuroda, N., Sugimoto, T., Fujimoto, J.
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Journal Title
J. Hepatobiliary Pancreat. Surg. 13・5
Pages: 363-369
Description
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[Journal Article] Bolckage of HGF/c-Met system by gene therapy (adenovirus-mediated NK4 gene) suppresses hepatocellular carcinoma in mice.2006
Author(s)
Son, G., Hirano, T., Seki, E., limuro, Y., Nukiwa, T., Matsumoto, K., Nakamura, T., Fujimoto, J.
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Journal Title
J. Hepatol. 45・5
Pages: 688-695
Description
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[Journal Article] Contribution of Toll-like receptor/myeloid differentiation factor 88 signaling to murine liver regeneration.2005
Author(s)
Seki, E., Tsutsui, H., limuro, Y., Naka, T., Son, G, Akira, S., Kishimoto, T., Nakanishi, K., Fujimoto, J.
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Journal Title
Hepatology 41・3
Pages: 443-450
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Oncostatin M inhibits proliferation of rat oval cells, OC15-5, inducing differentiation into hepatocytes.2005
Author(s)
Okaya, A., Kitanaka, J., Kitanaka, N., Satake, M., Kim, Y., Terada, K., Sugiyama, T., Takemura, M., Fujimoto, J., Terada, N., Miyajima, A., Tsujimura, T.
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Journal Title
Am. J. Pathol. 166・3
Pages: 709-719
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ameliorating effect of hepatocyte growth factor on inflammatory bowel disease in a murine model.2005
Author(s)
Oh, K., limuro, Y., Takeuchi, M., Kaneda, Y., Iwasaki, T., Terada, N., Matsumoto, T., Nakanishi, K., Fujimoto, J.
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Journal Title
Am. J. Physiol. Gastrointest Liver Physiol 288・4
Pages: G729-G735
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nuclear factor-KB inactivation in the rat liver ameliorates short term total warm ischaemia/reperfusion injury.2005
Author(s)
Suetsugu, H., limuro, Y., Uehara, T., Nishio, T., Harada, N., Yoshida, M., Hatano, E., Son, G, Fujimoto, J., Yamaoka, Y.
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Journal Title
Description
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[Journal Article] A Novel 3D hepatectomy simulation based on liver circulation : application to liver resection and transplantation.2005
Author(s)
Saito, S., Yamanaka, J., Miura, K., Nakao, N., Nagao, T., Sugimoto, T., Hirano, T., Kuroda, N., limuro, Y., Fujimoto, J.
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Journal Title
Hepatology 41・6
Pages: 1297-1304
Description
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