2007 Fiscal Year Final Research Report Summary
Identification and validation of tumor biomarkers for urogenital cancer using proteomic analysis of cell culture supernatant
| Project/Area Number |
17390439
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKAMURA Eijiro Kyoto University, Graduate school of medicine, associate professor (90293878)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Osamu Kyoto university, Graduate school of medicine, professor (90260611)
KAMOTO Toshiyuki Kyoto university, Graduate school of medicine, associate professor (00281098)
NISHIYAMA Hiroyuki Kyoto university, Graduate school of medicine, associate professor (20324642)
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| Project Period (FY) |
2005 – 2007
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| Keywords | urogenital cancer / tumor maker / shotgun proteomics / cell culture supernatant |
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| Research Abstract |
New proteomic approach for the identification of proteins that are potentially involved in the tumor invasion of bladder cancer.
We searched for the candidate proteins by comparing the profiles of secreted proteins among the poorly invasive human bladder carcinoma cell line and the highly invasive cell line. The proteins isolated from cell culture supernatants were identified by shotgun proteomics. We found that CXCL1 modulates the invasive abilities of bladder cancer cells and. this chemokine maybe a potential candidate of urinary biomarker for invasive bladder cancer and a possible therapeutic target for preventing tumor invasion.
New proteomic approach for the identification and validation of novel serum tumor makers for renal cell carcinoma.
We established a new method fir analyzing the secreted proteins in cell culture supernatants by using serum-free media and compared the proteome among 786-O RCC (VHL-/-) subclones stably transfected with either a plasmid encoding wild-type pVHL or an empty expression plasmid. By using this method, we identified three different VHL regulated secreted proteins (PAI-1,IGFBP-3 and clusterin). IGFBP-3 is elevated significantly in the serum from patients with ccRCC comparing with that from normal controls. On the other hand, we had done the preliminary analysis of "serum proteome" of 4 patients with sporadic clear cell RCC before and after the nephrectomy by using novel multidimensional shotgun proteomics. A total of 6 proteins were identified as candidate markers that were detected before the operation, but disappeared after the procedure. Two of them were biddy expressed in tumor specimens comparing with that of normal adjacent normal tissue. Now we perform further validation of these candidate proteins.
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[Journal Article] Secreted CXCL1 Is a Potential Mediator and Marker of the Tumor Invasion of Bladder Cancer2008
Author(s)
Kwanishi, H., Matsui, Y., Ito, M., Watanabe, J., Takahashi, T., Nishizawa, K., Nishiyama, H., Kamoto, T., Mikami, Y., Tanaka, Y., Jung, G., Akiyama, H., Nobumasa, H., Guilford, P., Reeve, A., Okuno, Y., Tsujimoto, G., Nakamura, E., Ogawa, O
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Journal Title
Clin Cancer Res 14
Pages: 2579-87
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] STAT3 polymorphism predicts the interferon-response in patients with metastatic renal cell carcinoma2007
Author(s)
N., Ito, M., Eto, E., Nakamura, A., Takahashi, T., Tsukamoto, H., Toma, H., Nakazawa, Y., Hirao, H., Uemura, S., Kagawa, H., Kanayama, Y., Nose, N., Kinukawa, T., Nakamura, N., Jinnai, T., Seki, M., Takamatsu, Y., Masui, S., Naito, Osamu, Ogawa
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Journal Title
J Clin Onco 125
Pages: 2785-2791
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Kaelin. Clusterin is a secreted marker for a hypoxia-inducible factor-independent function of Von Hippel-Lindau tumor suppressor protein2006
Author(s)
Nakamura, P., Abeu-e-Lima, Y., Awakura, T., Inoue, T., Kamoto, O., Ogawa, H., Kotani, T., Manabe, G., Zhang, K., Kondo, V., Nose, WG
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Journal Title
Am J Pathol 168
Pages: 574-584
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neuronal Apoptosis linked to EglN3 Prolyl Hydroxylase and Familial Pheochromocytoma Genes: Developmental Culling and Cancer.2005
Author(s)
Lee S, Nakamura E, Yang H, Wei W, Linggi MS, Sajan MP, Farese RV, Freeman RS, Carter BD, Kaelin WG, and Schlisio S.
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Journal Title
Cancer Cell 8
Pages: 155-167
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Neuronal Apoptosis linked to EgIN3 Prolyl Hydroxylase and Familial Pheochromocytoma Genes : Developmental Culling and Cancer. (Co-First Author)2005
Author(s)
S., Lee, E., Nakamura, H., Yang, W., Wei, MS., Linggi, MP., Sajan, RV., Farese, RS., Freeman, BD., Carter, WG., Kaelin, S., Schlisio
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Journal Title
Cancer Cells 8
Pages: 155-167
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Secreted CXCL1 is a Mediator and Marker of the Tumor Invasion of Bladder Cancer2007
Author(s)
Kwanishi, H., Nakamura, E., Nishiyama, H., Kamoto, T., Ogawa, O
Organizer
2007 annual congress meeting of AUA
Place of Presentation
Anahaim, USA
Year and Date
20070519-24
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of serum biomarkers of renal cell carcinoma by proteomic analysis2006
Author(s)
Yamasaki, T., Nakamura, E., Kwanishi, H., Awakura, Y., Kamba, T., Ito, N., Kamoto, T., Ogawa, O
Organizer
65th annual meeting of Japanese Cancer Association
Place of Presentation
yokohama
Year and Date
2006-09-29
Description
「研究成果報告書概要(欧文)」より
