Elucidation of the mechanism of ischemia/reperfusion injury of the lung and development of preventive and therapeutic maneuvers

2008 Fiscal Year Final Research Report

• Project/Area Number: 17390481
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: IMAI Takasuke Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 教授 (60091964)
• Co-Investigator(Kenkyū-buntansha): 倉田 俊一 東京医科歯科大学, 難治疾患研究所, 准教授(遺伝生化学) ; 江石 義信 東京医科歯科大学, 大学院・医歯学総合研究科, 教授(病理学)
• Project Period (FY): 2005 – 2008
• Keywords: 移植 / 再生医療 / 細胞 / 組織 / 生理学 / 虚血再環流傷害 / 肺循環

Research Abstract

片側肺を虚血、対側肺をコントロールとして用いることのできる遊離環流肺標本の確立、本モデルを用いて正常組織酸素、炭酸ガス分圧下で虚血傷害がoxygen radaicals, TNF-の産生により起こることを実証した。さらに、肺静脈圧を一定陽圧に保つことにより虚血傷害が阻止できることを見出した。また、虚血傷害により傷害肺からはTNF-, oxygen radicalsが放出されて、対側肺(遠隔臓器の代表)の透過性亢進を来すことを立証し、肺傷害による多臓器傷害発現機序の研究の端緒を開いた。

Research Products

• [Journal Article] Salavage of nonischemic control lung from injury by unilateral ischemic lung with apocynin, a nicotinamide adenine dinucleotide phosphate(NADPH)oxidase inhibitor, in isolated perfused rat lung (2008)
  • Author(s): Chenting Zhu, Aishan Bilali, Gabriela S Georgieva, Shunichi Kurata, Chieko Mitaka, Takasuke Imai
  • Journal Title: Translationa Research 152 Pages: 273-282
  • Peer Reviewed
• [Journal Article] Nonischemic lung injury by mediators from unilateral ischemic reperfused lung : ameliorating effect of tumor necrosis factor-α-converting enzyme inhibitor (2007)
  • Author(s): Gabriela S Georgieva, Shunichi Kurata, Satoshi Ikeda, Yoshinobu Eishi, Chieko Mitaka, Takasuke Imai
  • Journal Title: SHOCK 27 Pages: 84-90
  • Peer Reviewed
• [Journal Article] Prevention of ischemia reperfusion injury by positive pulmonary venous pressure in isolated rat lung (2006)
  • Author(s): Gabriela S Georgieva, Shunichi Kurata, Satoshi Ikeda, Shu Teng, Iyoko Katoh, Yoshinobu Eishi, Chieko Mitaka, Takasuke Imai
  • Journal Title: SHOCK 25 Pages: 66-72
  • Peer Reviewed
• [Presentation] TNF-α induction on respiratory epithelium in isolated, unilaterally hyperventilated rat lung (2009)
  • Author(s): Aishan Bilali, Satoshi Ikeda, Shunichi Kurata, Takasuke Imai
  • Organizer: 第36回日本集中治療医学会学術集会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 2009-02-26
• [Presentation] Induction of TNF-α on respiratory epithelium in unilateral hyperventilated lung an increased permeability by the recirculated perfusate in control lung maintained at CPAP(2.5 cmH20)in isolated, separately ventilated, perfused rat lung (2009)
  • Author(s): Aisha Bilali, Chenting Zhu, Gabriela Georgieva, Shunichi Kurata, Satoshi Ikeda, Takasuke Imai
  • Organizer: 38^<th> Critical Care Congress
  • Place of Presentation: Gaylord Opryland Resort and Convention Center, Nahville, Tennessee, USA
  • Year and Date: 2009-02-01
• [Presentation] Unilateral ischemic lung induces contralateral lung injury by liberating mdiators in cirulation Symposium "Acute Respiratory Failure and remote organ damage" (2008)
  • Author(s): Gabriela S Georgieva, Shunichi Kurata, Aisan Bilari, Chenting Zhu, Takasuke Imai
  • Organizer: 第35回日本集中治療医学会学術集会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2008-02-16
• [Presentation] Injury of CPAP lung by mediators from isolated perfused unilateral hyperventilated rat lung (2008)
  • Author(s): Bilali Aishan, Zhu Chenting, Georgieva S Gabriela, Kurata Shunichi, Imai Takasuke
  • Organizer: 第35回日本集中治療医学会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2008-02-14
• [Presentation] The effect of TNF-α on microvascular permeability in an isolated perfused lung (2008)
  • Author(s): Georgieva Gabriela S, Kurata Shunichi, Bilali Aishan, Zhu Chengting, Imai Takasuke
  • Organizer: 第35回日本集中治療医学会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2008-02-14
• [Presentation] Injury of CPAP lung by mediators from isolated perfused unilateral hyperventilated rat lung (2008)
  • Author(s): Aishan Bilali, Chenting Zhu, Gabriela S Georgieva, Takasuke Imai
  • Organizer: 37^<th> Critical Care Congress
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2008-02-03
• [Presentation] Effect of positive(5 mmHg)pulmonary venous pressure on isolated ischemic-reperfused rat lungs maintained at CPAP (2007)
  • Author(s): Georgieva GS, Kurata S, Zhu C, Bilali A, Imai T
  • Organizer: 20^<th> Annual Congress of European Society of Intensive Care Medicine
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2007-10-08
• [Presentation] Ameliorating effect of combination of apocynin and TACEI on ischemia/reperfusion(I/R)injury (2007)
  • Author(s): Chenting Zhu, Bilali Aishan, Gabriela Georgieva, Shunichi Kurata, Takasuke Imai
  • Organizer: 第34回日本集中治療医学会学術集会
  • Place of Presentation: 神戸国際展示場、神戸
  • Year and Date: 2007-03-02
• [Presentation] Induction of cytokine mRNA and edema in isolated perfused unilateral hyperventilated rat lung (2007)
  • Author(s): Bilali Aishan, Chenting Zhu, Gabriela Georgieva, Shunichi Kurata, Takasuke Imai
  • Organizer: 第34回日本集中治療医学会学術集会
  • Place of Presentation: 神戸国際展示場、神戸
  • Year and Date: 2007-03-02
• [Presentation] High tidal volume(TV)ventilation induced cytokine mRNA and edema in hyperventilated lung in isolated perfused unilateral hyperventilated lung (2007)
  • Author(s): Aishan Bilali, Chenting Zhu, Gabriela S Georgieva, Shunichi Kurata, Takasuke Imai
  • Organizer: 36^<th> Critical Care Congress
  • Place of Presentation: Gaylord Palms Resort and Cponvention Center Orlando, Florida, USA
  • Year and Date: 2007-02-18
• [Presentation] Ameliorating effect of combination of NADPH oxidase inhibitor(Apocynin)and TNF-α converting enzyme inhibitor(TACEI)on ishcmeia/reperfusion injury (2007)
  • Author(s): Chenting Zhu, Aishan Bilali, Gabriela S Georgieva, Shunichi Kurata, Takasuke Imai
  • Organizer: 36^<th> Critical Care Congress
  • Place of Presentation: Gaylord Palms Resort and Cponvention Center Orlando, Florida, USA
  • Year and Date: 2007-02-18
• [Presentation] Amelioration of ischemia-reperfusion(I/R)injury by NADPH oxidase inhibitor in isolated perfused rat lung (2006)
  • Author(s): Chenting Zhu, Asahn Bilali, Gabriela S Georgieva, Shunichi Kurata, Takasuke Imai
  • Organizer: 第34回日本救急医学会学術集会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2006-10-30
• [Presentation] Development of a novel model for high tidal volume lung injury (2006)
  • Author(s): Aishan Bilali, Chenting Zhu, Georgieva Gabriela, Shunichi Kurata, Takasuke Imai
  • Organizer: 第34回日本救急医学会学術集会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2006-10-30
• [Presentation] TNF-α converting enzyme inhibitor(TACEI)decreases control right lung injury induced by I/R left lung (2006)
  • Author(s): Gabriela S Georgieva, Takasuke Imai
  • Organizer: 第33回日本集中治療医学会学術集会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 2006-03-02
• [Presentation] Tumor necrosis factor-α converting enzyme inhibitor(TACEI)ameliorates control right lung(RL)injury by humoral mediators liberated from unilateral ischemia/reperfused(I/R)left lung(LL) (2006)
  • Author(s): Gabriela S Georgieva, Takasuke Imai, Shunichi Kurata
  • Organizer: 35^<th> Critical Care Congress
  • Place of Presentation: Moscone west convention center, SF, CA, USA
  • Year and Date: 2006-01-08
• [Presentation] Ischemic reperfused left lung(LL)increases expression of inflammatory cytokines mRNA in the control right lung(RL) (2005)
  • Author(s): Gabriela S Georgieva, Takasuke Imai, Shunichi Kurata
  • Organizer: 第33回日本救急医学会学術集会
  • Place of Presentation: 大宮ソニックシテイ、大宮
  • Year and Date: 2005-10-27