2007 Fiscal Year Final Research Report Summary
Molecular Evolutional, Geographical, Population Genetical analyses of Wild SilkMoth including Bombyx mandarina distribute in East Asian region
| Project/Area Number |
17405023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied entomology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
NAKAJIMA Yumiko University of the Ryukyus, Center of Molecular Biosciences, Associate Professor (70244340)
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| Co-Investigator(Kenkyū-buntansha) |
BANNO Yutaka Faculty of Agriculture Kyushu University, Department of Genetic Resource Technology, Associate Professor (50192711)
YUKUHIRO Keniji University of the Ryukyus, Chief Researcher (50343992)
ITOH Masanobu Kyoto Institute of Technology, Graduate School of Science and Technology, Associate Professor (60221082)
KAJIURA Zenta Shinshu University, Faculty of Textile Science and Technology, Associate Professor (10224403)
SAHARA Ken Hokkaido University, Faculty of Agriculture, Assistant Professor (30241368)
KUSAKABE Takahiro Kyushu University, Faculty of Agriculture, Associate Professor (30253595)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Bombyx mandarina / Wild Silk moth / mariner-like-elements (MLEs) / Mitochondria Cox1 gene / ALP gene (Alp-m, Alp-s) / Intergenic region / Vitellogenin gene / rDNA region |
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| Research Abstract |
We have analyzed the molecular level of polymorphism in Bombyx mandarina, some other wild silk moth species distribute in wide range of East Asian region by comparing some genes or DNA marker regions among them. B. mori molybdenum cofactor sulfurase gene (og) whose mutants cause translucent skin because of lack or deficiency of accumulation of uric acid under the skin in B. mori. Og genes from Japanese mandarina have wide variety and different from the group of B. mori. We conducted an analysis of mitochondrial coxl gene of B. mori and found low level of sequence diversity relative to that of the og gene. Alkaline phosphatase (Alp) genes are tandemly duplicated in B. mori. The Alp genes (Alp-m, Alp-s) share about 60% identity in the nucleotide sequences, but structure of the intervening region is highly polymorphic with large in/del from strain to strain. In this study we found that the Alp genes were shown to be duplicated in B. mandarina, similarly as in B. mori, in all four gemones. The structure of the intervening region in B. mandarina suggested that the most recent common ancestor of the current silk worm strains and wild B. mandarina existed in China. From the analysis of Cecropia-ITR-MLEs, most of the MLE sequences from B. mandarina in the main island of Japan composed one unique group (Japanese type); on the other hand, the sequences from the Chinese and Korean specimens settled down in another group (Continental type). We have isolated the active type of transposable element (Bmamat1) from the locus of BmTNML of Korean and Japanese mandarina. Vitellogenin genes of B. mandarina are closer to B. mori than other wild silk moth.
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[Journal Article] A novel maT-type transposable element. BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6.2008
Author(s)
Kawanishi, Y., Morimoto, M., Bammho Y, Nho, S. K., Maekawa, H., Nakajima, Y.
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Journal Title
Journal of Insect Biotechnology and Sericology 77(1)
Pages: 45-52
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Purification and cDNA Cloning of Vitellogenin of the Wild Silkworm, Saturnia japonica (Lepidoptera: Saturniidae)2008
Author(s)
Meng, Y., Liu, C, Shiomi, K., Nakagaki, M., Banno, Y ., Kajiura, Z. (2008)
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Journal Title
J. of Insect Biotechnology and Sericology 77 (1)
Pages: 35-44
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel war-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6.2008
Author(s)
Kawanishi, Y., Morimoto, M., Banno, Y., Nho, S. K., Maekawa, H., Nakajima, Y.
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Journal Title
J. of Insect Biotechnology and and Sericology 77 (1)
Pages: 45-52
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sequence comparison of mariner-like elements among the population of Bombyx mandarina inhabiting China, Korea, and Japan.2007
Author(s)
Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Nho, S. K., Maekawa, H., Nakaiima, Y.
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Journal Title
J. of Insect Biotechnology and Sericology 76(2)
Pages: 79-87
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Sequence comparison of mariner-like elements among the population of Bombyx mandarina inhabiting China, Korea, and Japan.2007
Author(s)
Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Nho, S. K., Maekawa, H., Nakajima, Y.
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Journal Title
J. of Insect Biotechnology and Sericology 76 (2)
Pages: 79-87
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] FISH analysis of the W chromosome in Bombyx mandarina and several other species of Lepidontera by means of B. mori W-BAC probes. 6. Meng, Y., Liu, C, Shiomi, K., Nakagaki, M.2007
Author(s)
Yoshido, A., Yasukochi, Y, Marec, E, Abe, H., Sahara, K.
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Journal Title
J. of Insect Biotechnology and Sericology 76 (1)
Pages: 1-7
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic variations in the vitellogenin of Japanese populations of the wild silkworm Bombyx mandarina.2006
Author(s)
Meng, Y., Liu, C, Shiomi, K., Nakagaki, M., Banno, Y., Kajiura, Z.
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Journal Title
J. of Insect Biotechnology and Sericology 75(3)
Pages: 127-134
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Vitellogenin gene organization of Antheraea yamamai and promoter activity analysis.
Author(s)
Meng, Y., Liu, C, Zhao, A., Shiomi, K., Nakagaki, M., Kajiura, Z.
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Journal Title
Int. J. of Wild Silkmoth & Silk. 11
Pages: 29-40
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Sequence comparison of the mariner-Iike-elements (MLEs)and their inserts among the populations of some species inhabiting in East Asian region.2007
Author(s)
Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Nho, S. K., Tanaka, C, Tanaka, J., Maekawa, H., Nakajima, Y.
Organizer
International Congress of Insect Biotechnologyand Industry (ICIBI)
Place of Presentation
Daegu, 韓国, EXCO
Year and Date
20070819-20070824
Description
「研究成果報告書概要(和文)」より
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[Presentation] Sequence comparison of the mariner-like-elements (MLEs) and their inserts among the populations of some species, inhabiting in East Asian region. (Invited speaker)2007
Author(s)
Kawanishi, Y., Takaishi, R.,Banno, Y, Fujimoto, H., Nho, S. K., Tanaka, C, Tanaka, J., Maekawa, H., Nakajima, Y.
Organizer
International Congress of Insect Biotechnology andIndustry (ICIBI)
Place of Presentation
EXCO, Daegu, Republic of Korea.
Year and Date
20070819-20070824
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Sequence Comparison of mariner-like-elements and inserted sequences into them among Bombyx mandarina inhabiting in China, Korea and Japan. (Invited speaker)2005
Author(s)
Kawanishi, Y., Takaishi, R., Banno, Y., Fujimoto, H., Maekawa, H., Nakaiima. Y.
Organizer
5th Asia-Pacific Congress of Entomology
Place of Presentation
Cheju, Republic of Korea.
Year and Date
20051018-20051021
Description
「研究成果報告書概要(欧文)」より
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