Epidemiological study on animal tick-borne protozoan diseases in Korea and China

2008 Fiscal Year Final Research Report

• Project/Area Number: 17405043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: XUAN Xuenan Obihiro University of Agriculture and Veterinary Medicine, 原虫病研究センター, 教授 (10292096)
• Co-Investigator(Kenkyū-buntansha): FUJISAKI Kozo 鹿児島大学, 農学部, 教授 (00292095) ; IGARASHI Ikuo 帯広畜産大学, 原虫病研究センター, 教授 (80159582) ; YOKOYAMA Naoaki 帯広畜産大学, 原虫病研究センター, 准教授 (80301802) ; IWATA Akira 財団法人日本生物科学研究所, 主任研究員 (70193745) ; HIRATA Haruyuki 酪農学園大学, 獣医学部, 准教授 (70381077)
• Co-Investigator(Renkei-kenkyūsha): FUJISAKI Kozo 鹿児島大学, 農学部, 教授 (00292095) ; IGARASHI Ikuo 帯広畜産大学, 原虫病研究センター, 教授 (80159582) ; YOKOYAMA Naoaki 帯広畜産大学, 原虫病研究センター, 准教授 (80301802) ; IWATA Akira , 財団法人日本生物科学研究所, 主任研究員 (70193745) ; HIRATA Haruyuki 酪農学園大学, 獣医学部, 准教授 (70381077)
• Project Period (FY): 2005 – 2008
• Keywords: 原虫感染症 / ダニ媒介 / バベシア原虫 / 血清診断 / 遺伝子診断 / 疫学調査 / 韓国 / 中国

Research Abstract

本研究は、日本と地理的にもっとも近い韓国及び朝鮮半島と国境を接している中国東北地方におけるダニ媒介性動物原虫感染症の流行の実態を明らかにし、日本の輸入検疫体制強化と韓国及び中国の原虫病診断技術の向上と普及に資することを目的として実施した。得られた主な成果として、(1)新規血清・遺伝子診断法の確立、(2)新規治療・予防法の開発、(3)韓国・中国の一部地域における牛・馬・犬などのバベシア原虫感染症の流行実態の解明、(4)韓国・中国の動物検疫関係機関への技術移転などが挙げられる。

Research Products

• [Journal Article] Genetic diversity of two selected antigen loci in Babesia gibsoni Asian genotype obtained from Japan and Jeju island of South Korea (2009)
  • Author(s): Jia, H., Aboge, G.O., Terkawi, M. A., Goo, Y. K., Nishikawa, Y., Kuriki, K., Lee, K. K.,Jang, H. K., Kim, S., Fujisaki, K., Xuan, X.
  • Journal Title: Vet. Parasitol 162 Pages: 142-146
  • Peer Reviewed
• [Journal Article] Cloning, expression and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase : inhibitory effect of antifolates on its catalytic activity and the parasiteproliferation. (2008)
  • Author(s): Aboge, G., Jia, H., Terkawi, M., Goo, Y.K., Nishikawa, Y., Sunaga, F., Namikawa, K., Igarashi, I., Suzuki, H., Fujisaki, K., Xuan, X.
  • Journal Title: Antimicrob. Agents Chemother 52 Pages: 4072-4080
  • Peer Reviewed
• [Journal Article] Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples (2008)
  • Author(s): Kim, C.M., Blanco, L.B., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.
  • Journal Title: Vet. Parasitol 151 Pages: 158-163
  • Peer Reviewed
• [Journal Article] Development of a rapid immunochromatographic test for simultaneous serodiagnosis of bovine babesioses caused by Babesia bovis and Babesia bigemina. (2008)
  • Author(s): Kim, C.M., Blanco, L. B., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.Kim, C. M., Blanco, L.B., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.
  • Journal Title: Am. J. Trop. Med. Hyg 78 Pages: 117-121
  • Peer Reviewed
• [Journal Article] Development of Taqman-based real-time PCR assays for diagnostic detection of Babesia bovis and Babesia bigemina. (2007)
  • Author(s): Kim, C., Iseki, H., Herbas, M.S., Yokoyama, N., Suzuki, H., Xuan, X., Fujisaki, K., Igarashi, I.
  • Journal Title: Am. J. Trop. Med. Hyg. 77 Pages: 837-841
  • Peer Reviewed
• [Journal Article] Development of two immunochromatographic tests for the serodiagnosis of bovine babesiosis (2007)
  • Author(s): Kim, C., Alhassan, A., Verdida, R. A., Yokoyama, N., Xuan, X., Fujisaki, K., Kawazu, S. I., Igarashi, I.
  • Journal Title: Vet. Parasitol 148 Pages: 137-143
  • Peer Reviewed
• [Journal Article] Ashared antigen among Babesia species : Ribosomal phosphoprotein P0 as a universal babesial vaccine candidate. (2007)
  • Author(s): Terkawi, M. A., Jia, H., Gabriel, A., Goo, Y. K., Nishikawa, Y., Yokoyama, N., Igarashi, I., Fujisaki, K., Xuan, X.
  • Journal Title: Parasitol. Res 102 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Molecular characterization of Rhipicephalus (Boophilus) microplus Bm86 homologue from Haemaphysalis longicornis ticks (2007)
  • Author(s): Liao, M., Zhou, J., Hatta, T., Umemiya, R., Miyoshi, T., Tsuji, N., Xuan, X., Fujisaki, K.
  • Journal Title: Vet. Parasitol 146 Pages: 148-157
  • Peer Reviewed
• [Journal Article] Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B. microti infection in mice. (2007)
  • Author(s): Terkawi, M. A., Jia, H., Zhou, J., Lee, E. G., Igarashi, I., Fujisaki, K., Nishikawa, Y., Xuan, X.
  • Journal Title: Vaccine 25 Pages: 2027-2035
  • Peer Reviewed
• [Journal Article] Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs (2007)
  • Author(s): Fukumoto, S., Tamaki, Y., Okamura, M., Bannai, H., Yokoyama, N., Suzuki, T., Igarashi, I., Suzuki, H., Xuan, X.
  • Journal Title: Vaccine 25 Pages: 1334-1341
  • Peer Reviewed
• [Journal Article] Detection of DNA closely related to 'Candidatus Rickettsia principis' in Haemaphysalis danieli recovered from cattle in Xinjiang Uygur Autonomous Region area, China (2007)
  • Author(s): Chahan, B., Jian, Z., Miyahara, K., Tanabe,S., Xuan, X., Sato, Y., Moritomo, T., Nogami, S., Mikami, T., Maruyama, S., Inokuma, H.
  • Journal Title: Vet. Parasitol. 144 Pages: 184-187
  • Peer Reviewed
• [Journal Article] A Babesia gibsoni thrombospondin related anonymous protein (BgTRAP) is an immunodominant antigen involved in the erythrocyte invasion (2006)
  • Author(s): Zhou, J., Zhang, G., Fukumoto, S., Yokoyama, N., Suzuki, H., Igarashi, I., Fujisaki, K., Lin, J., Xuan, X.
  • Journal Title: Mol. Biochem. Parasitol 148 Pages: 190-198
  • Peer Reviewed
• [Journal Article] An immunochromatographic test for simultaneous serodiagnosis of both Babesia equi and B. caballi infections in horses (2006)
  • Author(s): Huang, X., Xuan, X., Verdida, R., Zhang, S., Yokoyama, N., Xu, L., Igarashi, I.
  • Journal Title: Clin. Vaccine Immunol 13 Pages: 553-555
  • Peer Reviewed
• [Journal Article] Evaluation enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infection (2006)
  • Author(s): Huang, X., Xuan, X., Yokoyama, N., Katayama, Y., Anzai, T., Igarashi, I.
  • Journal Title: Vet. Parasitol 140 Pages: 158-161
  • Peer Reviewed
• [Journal Article] Seroepidemiological evidence for the possible presence of Babesia (Theileria) equi and Babesia caballi infections in donkeys in Western Xinjiang, China (2006)
  • Author(s): Chahan, B., Zhang, S., Seo, J., Nakamura, C., Zhang, G., Bannai, H., Jian, Z., Inokuma, H., Tuchiya, K., Sato, Y., Kabeya, H., Maruyama, S., Mikami, T. ,Xuan, X.
  • Journal Title: J. Vet. Med. Sci 68 Pages: 753-755
  • Peer Reviewed
• [Journal Article] Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay (2006)
  • Author(s): Boonchit, S., Alhassan, A., Chan, B., Xuan, X., Yokoyama, N., Ooshiro, M., Goff, W.L., Waghela, S.D., Wagner, G., Igarashi, I.
  • Journal Title: Vet. Parasitol 137 Pages: 28-35
  • Peer Reviewed
• [Journal Article] Epidemiological study of equine piroplasmosis in Mongolia. (2005)
  • Author(s): Boldbaatar, D., Xuan, X., Battsetseg, B., Igarashi, I., Battur, B., Batsukh, Z., Bayambaa, B., Fujisaki, K.
  • Journal Title: Vet. Parasitol 127 Pages: 35-38
  • Peer Reviewed
• [Journal Article] Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis (2005)
  • Author(s): Hirata, H., Yokoyama, N., Xuan, X., Fujisaki, K., Suzuki, N., Igarashi, I.
  • Journal Title: Clin. Diagn. Lab. Immunol 12 Pages: 334-338
  • Peer Reviewed
• [Journal Article] Development of a practical immunochromatographic test with recombinant P50 for the diagnosis of Babesia gibsoni infection in dogs (2005)
  • Author(s): Verdida, R.A., Xuan, X., Fukumoto, S., Huang, X., Zhou, J., Igarashi, I., Claveria, F. G., Nagasawa, H.
  • Journal Title: Parasitology 131 Pages: 769-774
  • Peer Reviewed
• [Journal Article] Serological evidence of infection of Anaplasma and Ehrlichia in domestic animals in Xinjiang Uygur Autonomous Region area, China (2005)
  • Author(s): Chahan, B., Jian, Z., Xuan, X., Sato, Y., Kabeya, H., Tuchiya, K., Itamoto, K., Okuda, M., Mikami, T., Maruyama, S., Inokuma, H.
  • Journal Title: Vet. Parasitol 134 Pages: 273-278
  • Peer Reviewed
• [Presentation] Babesia microti 新規抗原BmP49の同定と性状解析 (2009)
  • Author(s): 大岡秀雄、五十嵐郁男、玄学南(その他4名)
  • Organizer: 第147 回日本獣医学会
  • Place of Presentation: 宇都宮
  • Year and Date: 2009-04-03
• [Presentation] バベシア症に対するアーテスネートの治療効果 (2009)
  • Author(s): 具潤景、藤崎幸蔵、玄学南(その他6名)
  • Organizer: 第147 回日本獣医学会
  • Place of Presentation: 宇都宮
  • Year and Date: 2009-04-03
• [Presentation] Diagnostic real-time PCR assay for the quantitative detection of Theileria equi blood samples (2009)
  • Author(s): Yokoyama, N., Xuan, X., Igarashi, I.(その他4名)
  • Organizer: 第145回日本獣医学会
  • Place of Presentation: 相模原
  • Year and Date: 2009-03-29
• [Presentation] タイにおけるウシバベシア症の疫学調査 (2008)
  • Author(s): 周麗佳、横山直明、玄学南、五十嵐郁男(その他3名)
  • Organizer: 第146 回日本獣医学会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-09-25
• [Presentation] Antifolate drugs target dihydrofolate reductase-thymidylate synthase of Babesia gibsoni (2008)
  • Author(s): Aboge G., Igarashi, I., Fujisaki, K., Xuan, X.(その他5名)
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-09-25
• [Presentation] Characterization of leucyl aminopeptidase kinase of Babesia gibsoni (2007)
  • Author(s): Jia, H., Fujisaki, K., Xuan, X.(その他5名)
  • Organizer: 第144回日本獣医学会
  • Place of Presentation: 江別
  • Year and Date: 2007-09-03
• [Presentation] Development of real-time PCR assays for detection of Babesia bovis and Babesia bigemina (2007)
  • Author(s): Kim, C., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.
  • Organizer: 第143回日本獣医学会
  • Place of Presentation: つくば
  • Year and Date: 2007-04-04
• [Presentation] Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B. microti in mice (2006)
  • Author(s): Terkawi, A., Jia, H., Xuan, X., Fujisaki, K.
  • Organizer: 第141回日本獣医学会
  • Place of Presentation: つくば
  • Year and Date: 2006-03-20
• [Presentation] ワクシニアウイルスベクターを用いた犬Babesia gibsoni TRAP 遺伝子の発現と性状解析 (2006)
  • Author(s): 中村千夏、玄学南(その他4名)
  • Organizer: 第141 回日本獣医学会
  • Place of Presentation: つくば
  • Year and Date: 2006-03-20
• [Presentation] フタトゲチマダニの臓器別完全長ESTデータベースの解析 (2005)
  • Author(s): 辻尚利、玄学南、藤崎幸蔵(その他12名)
  • Organizer: 第140回日本獣医学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2005-09-30
• [Presentation] Cloning and expression of Bm86 analogue from Haemaphysalis longicornis tick (2005)
  • Author(s): Liao, M., Xuan, X., Zhou, J., Fujisaki, K.
  • Organizer: 第140回日本獣医学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2005-09-30
• [Presentation] Identification of a novel gene encoding secreted antigen BgSA1 from Babesia gibsoni (2005)
  • Author(s): Jia, H., Fujisaki, K., Xuan, X.(その他6名)
  • Organizer: 第140 回日本獣医学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2005-09-30
• [Presentation] 中国新彊地域におけるウマ・ロババベシア原虫感染症の疫学調査 (2005)
  • Author(s): 玄学南、五十嵐郁男(その他10名)
  • Organizer: 第140回日本獣医学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2005-09-30
• [Remarks] 研究室のホームページにて関連研究活動・業績等を発信している
  • URL: http://www.obihiro.ac.jp/~geneticbiochem/
• [Patent(Industrial Property Rights)] ウマバベシア診断キット (2005)
  • Inventor(s): 五十嵐郁男、黄暁紅、玄学南
  • Industrial Property Number: 特願2005-8062
  • Filing Date: 20050000
• [Patent(Industrial Property Rights)] マダニのロイシンアミノペプチダーゼ (2005)
  • Inventor(s): 藤崎幸蔵、八田岳士、玄学南、辻尚利
  • Industrial Property Number: 特願2005-65550
  • Filing Date: 20050000
• [Patent(Industrial Property Rights)] バベシア・ギブソニの分泌抗原1タンパク質及びこのタンパク質をコードするDNA、並びにそれバベシア・ギブソニの分泌抗原1タンパク質及びこのタンパク質をコードするDNA、並びにそれらの利用 (2005)
  • Inventor(s): 玄学南、賈洪林、周金林、鈴木宏志、藤崎幸蔵、五十嵐郁男
  • Industrial Property Number: 特願2005-244508
  • Filing Date: 20050000