2007 Fiscal Year Final Research Report Summary
Study on pathogenesis of eosionophilia with MES congenic rat developing human eosinophilia
| Project/Area Number |
17500285
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Shinshu University |
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Principal Investigator |
MATSUMOTO Kiyoshi Shinshu University, Research Center for Human and Environmental Sciences, Associate Professor (40173893)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Masayki Shinshu University, School of Medicine, Associate Professor (60273190)
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| Project Period (FY) |
2005 – 2007
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| Keywords | eosinophilia / confronic rat / micrnsatellite-marker / genetic trait |
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| Research Abstract |
The MES rat, Dr. Matsumoto the applicant of this grant found out, spontaneously develops eosinophilia and eosinophil-related inflammatory lesions in many organs. As part of investigation into the cause of eosinophilia in MES rats, genetic and hematological analyses were carried out with congenic rats (BN.MES-eos1). As a first step, peripheral blood hematology (OHM bone marrow eosinophil count and gross pathology were carried out on the F2 progeny obtained by mating congenic (BN.MES-eosl) and MES rats. Of the 134 F2 animals, 79 (59%) showed white spot in the liver, which was histopathologically diagnosed as focal necrosis with eosinophil infiltration. Remarkably, almost all rats with sever white spot lesions had eosinophilic hyperplasia (20%<) in the bone marrow. Of these 88 rats with marrow eosinophilic hyperplasia, 37 rats did not show eosinophilia (<500/u L) in the peripheral blood. These findings of marrow eosinophilic hyperplasia without peripheral eoisonophilia were clinically similar to those in BN.MES-eos1 rats. To elucidate the genetic roles of hematological pathology, gene mapping study are carried out. As a results, it was found that the major locus for eosinophilia was located on chromosome 19 (Eosl) and another quantitative trait locus showing suggestive linkage for eosinophilia on choromosome 1 (Eos-g). Additionally, the findings of a third locus for esinophilia also located on chromosome 2 (EosE.0, suggesting that eosinophilia in MES is a rather complex genetic trait.
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