2007 Fiscal Year Final Research Report Summary
Analysis of the induction mechanisms of atherosclerosis using C-reactive protein transgenic rabbits
| Project/Area Number |
17500287
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kumamoto Health Science University (2007)
Saga University (2005-2006) |
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Principal Investigator |
MORIMOTO Masatoshi Kumamoto Health Science University, Dep. of Rehabilitation, Professor (90136482)
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| Co-Investigator(Kenkyū-buntansha) |
KITAJIMA Shuji Saga University, Analytical Research Center for Experimental Sciences, Associate professor (70284643)
FAN Jianglin Yamanashi University, Interdisciplinary Graduate School of Medical and Engineering, Professor (60272192)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Athelosclerosis / Lipid metabolism / C-reactive protein / Animal model / Transgene |
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| Research Abstract |
In this study, we made plivLE6-hCRP construct which inserted human C-reactive protein (h-CRP) cDNA in plivHEG/LE6 vector to express high h-CRP in liver specifically, and it was injected into rabbit fertilized egg pronuclei by microinjection method. After microinjection, 453 embryos were transplanted in fallopian tubes of the foster rabbits induced pseudopregnancy by hCG injection and they gave birth to 47 pups in all. PCR analysis revealed transgene was introduced in 5 of 47 pups, and we confirmed h-CRP expression in plasma of 2 rabbits (Line C205 and C215) survived until weaning. After expand of the each colony of C205 and C215, we examined the expression level of h-CRP in each transgenic line, and we confirmed that C205 and C215 showed 7.3±1.0 and 122.0±13.4 mg/dL, respectively. In comparison of plasma lipid profile such as total cholesterol, HDL-cholesterol and triglyceride, there were no differences between transgenic and non-transgenic rabbits.
For analysis of the induction mechanisms of atherosclerosis, transgenic and non-transgenic rabbits were fed cholesterol-rich diet for 16 weeks. The concentration of plasma cholesterol was measured weekly and adjusted to same level between 2 groups by changing the amount of cholesterol in the diet (0.075-0.3%). Cholesterol loading was finished already, and we have collected aortas and hearts from all rabbits for pathological analysis. At present, more detailed histopathological analysis is doing for preparation of manuscript.
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Research Products
(20 results)
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[Journal Article] Matrix metalloproteinase 12 is a factor that acceleratesthe initiation of atherosclerosis and stimulates the progression of fattystreaks to fibrous plaques in transgenic rabbits.2008
Author(s)
Yamada, S., Wang, K-Y., Tanimoto, A., Fan, J., Shimajiri, S., Kitajima, S., Morimoto, M., Tsutsui, M., Watanabe, T., Yasumoto, K., Sasaguri, Y.
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Journal Title
Am J Pathol 172
Pages: 1419-1429
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Re-establishment of Complement C6-deficeint RabbitColony by Cryopreserved Sperm Transported from Abroad.2007
Author(s)
Liu, E., Kitajima, S., Wiese, E., Reifenberg, E., Morimoto, M., Watanabe, T., Fan, J.
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Journal Title
Exp. Anim. 55(2)
Pages: 167-171
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Lp(a) enhances coronaryatherosclerosis in transgenic Watanabe heritable hyperlipidemic rabbits.2007
Author(s)
Kitajima, S., Jin, Y., Koike, T., Yu, Y, Liu, E., Morimoto, M., Shiomi, M., Marcovina, S., Watanabe, N., Fan, J.
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Journal Title
Atherosclerosis 193
Pages: 269-276
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Macrophage elastase(MMP-12) accelerates the progression ofatherosclerosis in transgenic rabbits.2006
Author(s)
Liang J., Liu E., Yu Y., Kitajima S., Koike T., Jin Y., Morimoto M., Watanabe T., Hatakeyama K., Asada Y., Sasaguri Y., Watanabe S., Fan J.
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Journal Title
Circulation 113
Pages: 1993-2001
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Establishment of a SPF colony of humanapo(a) transgenic rabbits by frozen-thawed embryo transfer.2005
Author(s)
Watanabe, N., Nakagawa, H., Kitajima, S., Liu, E., Morimoto, M, Watanabe, T, Fan, J.
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Journal Title
Exp. Anim. 54(4)
Pages: 353-357
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] C-reactive protein in atherosclerotic lesions : its origin andpathophysiological significance.2005
Author(s)
Sun, H., Koike, T., Ichikawa, T., Hatakeyama, K., Shiomi, M., Zhang, B., Kitajima, S., Morimoto, M., Watanabe, T., Asada, Y., Chen, YE., Fan, J.
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Journal Title
Am. J. Pathol. 167
Pages: 1139-1148
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Perticipation of macrophage erastase(MMP-12) in atherosclerosis.2005
Author(s)
Liang, J., Kitajima, S., Wang, X., Koike, T., Jin Y., Morimoto, M., Fan, J.
Organizer
The 1st International Conference on'Transgenic Rabbits.
Place of Presentation
Japan
Year and Date
2005-06-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Human LPL transgenic rabbits-Application of transgenic rabbit to human metabolic syndrome.2005
Author(s)
Liu, E., Kitajima, S., Koike, T., Morimoto, M., Watanabe, T., Fan, J.
Organizer
The 1st International Conference on Transgenic Rabbits.
Place of Presentation
Japan
Year and Date
2005-06-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Enhanced atherosclerosis in transgenic Watanabe heritable hyperlipidemic rabbits expressing human lipoprotein lipase.2005
Author(s)
Koike, T., Shiomi, M., Liang, J., Kitajima, S., Morimoto, M., Watanabe, T., Yamada, N., Fan, J.
Organizer
The 1st International Conference on Transgenic Rabbits.
Place of Presentation
Japan
Year and Date
2005-06-18
Description
「研究成果報告書概要(欧文)」より
