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2008 Fiscal Year Final Research Report

Monitoring of mitochondrial DNA mutations using fluorescence correlation spetrcoscopy

Research Project

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Project/Area Number 17500299
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionYamagata University

Principal Investigator

NOMURA Yasutomo  Yamagata University, 大学院・医学系研究科, 准教授 (80237883)

Co-Investigator(Renkei-kenkyūsha) KINJO Masataka  北海道大学, 大学院・先端生命科学研究院, 教授 (70177971)
Research Collaborator NAKAMURA Takao  山形大学, 大学院・医学系研究科, 教授 (00142654)
FENG Zhonggang  山形大学, 大学院・理工学研究科, 准教授 (10332545)
SAKAMOTO Mitsuru  山形大学, 大学院・理工学研究科, 学生
OKAMOTO Sin-ichiro  山形大学, 大学院・理工学研究科, 学生
FUCHIGAMI Hirobumi  山形大学, 大学院・理工学研究科, 学生
ARAGANE Atsuo  山形大学, 大学院・理工学研究科, 学生
FUJIWARA Hiroyuki  山形大学, 大学院・理工学研究科, 学生
ITOU Kohei  山形大学, 大学院・理工学研究科, 学生
MATSUMOTO Satoru  山形大学, 大学院・理工学研究科, 学生
TAKAYAMA Toshifumi  山形大学, 大学院・医学系研究科, 学生
GOTO Takehito  山形大学, 大学院・医学系研究科, 学生
Project Period (FY) 2005 – 2008
KeywordsミトコンドリアDNA / 蛍光相関分光法 / ブラウン運動 / 拡散定数 / 共焦点光学系 / レーザー
Research Abstract

過酸化水素処理した細胞からミトコンドリアDNAを精製して、そのPCR産物の拡散係数から酸化的ストレスによる損傷の程度を推定できることを指摘した。さらにミトコンドリア遺伝子変異のホットスポットに注目して、制限酵素処理による断片長分布を蛍光相関分光の多成分解析によって簡便に推定できるようにした。

  • Research Products

    (52 results)

All 2009 2008 2007 2006 2005

All Journal Article (45 results) (of which Peer Reviewed: 45 results) Presentation (3 results) Book (4 results)

  • [Journal Article] Mitochondria-targeted green fluorescent protein for quantitative monitoring of mitochondrial morphology in living cells Lett2009

    • Author(s)
      Nomura Y Takayama T, Sato M, Takahash E, Feng Z, Nakamura T
    • Journal Title

      Drug Design Discov 6(4)(in press)

    • Peer Reviewed
  • [Journal Article] Fluorescence cross-correlation spectroscopy of plant proteins2009

    • Author(s)
      Muto H, Kinjo M, Yamamoto KT.
    • Journal Title

      Methods Mol Biol 479

      Pages: 203-15

    • Peer Reviewed
  • [Journal Article] Comparison of mRNA expression of transcriptional factors and intercalated disk constituent proteins between in vivo and cultured cardiomyocytes2008

    • Author(s)
      Nakamura T, Feng Z, Honda T, Nomura Y, Kitajima T, Umezu M
    • Journal Title

      J Artif Organs 11

      Pages: 134-140

    • Peer Reviewed
  • [Journal Article] The A-and B-type nuclear lamin networks : microdomains involved in chromatin organization and transcription2008

    • Author(s)
      Shimi T, Pfleghaar K, Kojima S, Pack CG, Solovei I, Goldman AE, Adam SA, Shumaker DK, Kinjo M, Cremer T, Goldman RD
    • Journal Title

      Genes Dev. 22(24)

      Pages: 3409-21

    • Peer Reviewed
  • [Journal Article] Cross-talk-free fluorescence cross-correlation spectroscopy by the switching method2008

    • Author(s)
      Takahashi Y, Nishimura J, Suzuki A, Ishibashi K, Kinjo M, Miyawaki A
    • Journal Title

      Cell Struct Funct 33(1)

      Pages: 143-50

    • Peer Reviewed
  • [Journal Article] Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking2008

    • Author(s)
      Noda Y, Horikawa S, Kanda E, Yamashita M,Meng H, Eto K, Li Y, Kuwahara M, Hirai K,Pack C, Kinjo M, Okabe S, Sasaki S.
    • Journal Title

      J Cell Biol 182(3)

      Pages: 587-601

    • Peer Reviewed
  • [Journal Article] In vivo quantitative analysis of PKA subuni tinteraction and cAMP level by dual color fluorescence cross correlation spectroscopy2008

    • Author(s)
      Park H, Pack C, Kinjo M, Kaang BK
    • Journal Title

      Mol Cells 26(1)

      Pages: 87-92

    • Peer Reviewed
  • [Journal Article] Stress-induced environmental changes in a single cell as revealed by fluorescence lifetime imaging2008

    • Author(s)
      Nakabayashi T, Nagao I, Kinjo M, Aoki Y, Tanaka M, Ohta N
    • Journal Title

      Photochem Photobiol Sci 7(6)

      Pages: 671-4

    • Peer Reviewed
  • [Journal Article] Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements2008

    • Author(s)
      Nakabayashi T, Wang HP, Kinjo M, Ohta N
    • Journal Title

      Photochem Photobiol Sci 7(6)

      Pages: 668-70

    • Peer Reviewed
  • [Journal Article] Regulated motion of glycoproteins revealed by direct visualization of a single cargo in the endoplasmic reticulum2008

    • Author(s)
      Nagaya H, Tamura T, Higa-Nishiyama A, Ohashi K, Takeuchi M, Hashimoto H, Hatsuzawa K, Kinjo M, Okada T, Wada I.
    • Journal Title

      J Cell Biol 180(1)

      Pages: 129-43

    • Peer Reviewed
  • [Journal Article] Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching2008

    • Author(s)
      Nagao I, Aoki Y, Tanaka M, Kinjo M.
    • Journal Title

      FEBS J. 275(2)

      Pages: 341-9

    • Peer Reviewed
  • [Journal Article] Direct quantification of gene expression using fluorescence correlation spectroscopy2007

    • Author(s)
      Nomura Y, Nakamura T, Feng Z, Kinjo M.
    • Journal Title

      Curr Pharm Biotech 8

      Pages: 286-290

    • Peer Reviewed
  • [Journal Article] Detection of antigen protein by using fluorescence cross-correlation spectroscopy and quantum-dot-labeled antibodies2007

    • Author(s)
      Fujii F, Kinjo M
    • Journal Title

      Chembiochem 8(18)

      Pages: 2199-203

    • Peer Reviewed
  • [Journal Article] Effect of cholesterol on diffusion in surfactant bilayers2007

    • Author(s)
      Pieper T, Markova S, Kinjo M, Suter D
    • Journal Title

      J Chem Phys 127(16)

      Pages: 165102

    • Peer Reviewed
  • [Journal Article] Preparation and characterization of thiacalix[4]arene coated water-soluble CdSe/ZnS quantum dots as a fluorescent probe for Cu2+ ions2007

    • Author(s)
      Jin T, Fujii F, Yamada E, Nodasaka Y, Kinjo M.
    • Journal Title

      Comb Chem High Throughput Screen 10(6)

      Pages: 473-9

    • Peer Reviewed
  • [Journal Article] Detection of prion protein immune complex for bovine spongiform encephalopathy diagnosis using fluorescence correlation pectroscopy and fluorescence cross-correlation spectroscopy2007

    • Author(s)
      Fujii F, Horiuchi M, Ueno M, Sakata H, Nagao I, Tamura M, Kinjo M
    • Journal Title

      Anal Biochem 370(2)

      Pages: 131-41

    • Peer Reviewed
  • [Journal Article] Dissociation of the insulin receptor and caveolin-1 complex by ganglioside GM3 in the state of insulin resistance2007

    • Author(s)
      Kabayama K, Sato T, Saito K, Loberto N, Prinetti A, Sonnino S, Kinjo M, Igarashi Y, Inokuchi J
    • Journal Title

      Proc Natl Acad Sci USA 104(34)

      Pages: 13678-83

    • Peer Reviewed
  • [Journal Article] Detection of polyglutamine protein oligomers in cells by fluorescence correlation spectroscopy2007

    • Author(s)
      Takahashi Y, Okamoto Y, Popiel HA, Fujikake N, Toda T, Kinjo M, Nagai Y
    • Journal Title

      J Biol Chem 282(33)

      Pages: 24039-48

    • Peer Reviewed
  • [Journal Article] Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy2007

    • Author(s)
      Shimizu M, Sasaki S, Kinjo M.
    • Journal Title

      Anal Biochem 366(1)

      Pages: 87-92

    • Peer Reviewed
  • [Journal Article] DNA microstructure based on self-assembly of 4-sticky-end olliday junctions in aqueous solution2007

    • Author(s)
      Kii H, Takagi T, Sasaki A, Okajima T, Kinjo M
    • Journal Title

      J Nanosci Nanotechnol 7(3)

      Pages: 726-9

    • Peer Reviewed
  • [Journal Article] Haploinsufficiency of 8p22 may influence cancer-specific survival in prostate cancer2007

    • Author(s)
      Matsuyama H, Oba K, Matsuda K, Yoshihiro S, Tsukamoto M, Kinjo M, Sagiyama K, Takei M, Yamaguchi A, Sasaki K, Naito K.
    • Journal Title

      Cancer Genet Cytogenet 174(1)

      Pages: 24-34

    • Peer Reviewed
  • [Journal Article] The novel cargo Alcadein induces vesicle association of kinesin-1 motor components and activates axonal transport2007

    • Author(s)
      Araki Y, Kawano T, Taru H, Saito Y, Wada S, Miyamoto K, Kobayashi H, Ishikawa HO, Ohsugi Y, Yamamoto T, Matsuno K, Kinjo M, Suzuki T
    • Journal Title

      EMBO J 26(6)

      Pages: 1475-86

    • Peer Reviewed
  • [Journal Article] Analysis of intranuclear binding process of glucocorticoid receptor using fluorescence correlation spectroscopy2007

    • Author(s)
      Mikuni S, Tamura M, Kinjo M
    • Journal Title

      FEBS Lett 581(3)

      Pages: 389-93

    • Peer Reviewed
  • [Journal Article] Detection of oxidative stress-induced mitochondrial DNA damage using fluorescence correlation spectroscopy2006

    • Author(s)
      Nomura Y, Fuchigami H, Kii H, Feng Z,Nakamura T, Kinjo M.
    • Journal Title

      Anal Biochem 350(2)

      Pages: 196-201

    • Peer Reviewed
  • [Journal Article] Quantification of size distribution of restriction fragments in mitochondrial genome using fluorescence correlation spectroscopy2006

    • Author(s)
      Nomura Y, Fuchigami H, Kii H, Feng Z, Nakamura T, Kinjo M
    • Journal Title

      Exp Mol Pathol 80

      Pages: 275-278

    • Peer Reviewed
  • [Journal Article] HIF-1α as a target for drug design in ischemic injury : effect of cobalt treatment on mitochondrial DNA damage in cells exposed to H2O22006

    • Author(s)
      Nomura Y, Fujiwara H, Ito K, Fuchigami H, Sato M, Takahashi M, Hozumi Y, Goto K, Fuchigami H, Feng Z, Nakamura T
    • Journal Title

      Lett Drug Design Discov 3(3)

      Pages: 172-174

    • Peer Reviewed
  • [Journal Article] In vivo oxygen imaging using green fluorescent protein2006

    • Author(s)
      Takahashi E, Takano T, Nomura Y, Okano S, Nakajima O, Sato M
    • Journal Title

      Am J Physiol-Cell Physiol 291

      Pages: 781-787

    • Peer Reviewed
  • [Journal Article] Constraint stress, microstructural characteristics and enhanced mechanical properties of a special fibroblast-embedded collagen construct2006

    • Author(s)
      Feng Z, Ishibashi M, Nomura Y, Kitajima T, Nakamura T
    • Journal Title

      Artif Organs 30

      Pages: 870-877

    • Peer Reviewed
  • [Journal Article] Construction of fibroblast-collagen gels with orientated fibrils induced by static or dynamic stress : toward the fabrication of small tendon grafts2006

    • Author(s)
      Feng Z, Tateishi Y, Nomura Y, Kitajima T, Nakamura T
    • Journal Title

      J Artif Organs 9(4)

      Pages: 220-225

    • Peer Reviewed
  • [Journal Article] Cytosolic chaperonin prevents polyglutamine toxicity with altering the aggregation state2006

    • Author(s)
      Kitamura A, Kubota H, Pack CG, Matsumoto G, Hirayama S, Takahashi Y, KimuraH, Kinjo M, Morimoto RI, Nagata K
    • Journal Title

      Nat Cell Biol 8(10)

      Pages: 1163-70

    • Peer Reviewed
  • [Journal Article] Dynamics of yeast prion aggregates in singleliving cells2006

    • Author(s)
      Kawai-Noma S, Ayano S, Pack CG, Kinjo M, Yoshida M, Yasuda K, Taguchi H.
    • Journal Title

      Genes Cells 11(9)

      Pages: 1085-96

    • Peer Reviewed
  • [Journal Article] Protein-protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy2006

    • Author(s)
      Oyama R, Takashima H, Yonezawa M, DoiN, Miyamoto-Sato E, Kinjo M, Yanagawa H
    • Journal Title

      Nucleic Acids Res 34(14)

      Pages: e102

    • Peer Reviewed
  • [Journal Article] Lateral mobility of membrane-binding proteins inliving cells measured by total internal reflection fluorescence correlation spectroscopy2006

    • Author(s)
      Ohsugi Y, Saito K, Tamura M, Kinjo M
    • Journal Title

      Biophys J. 91(9)

      Pages: 3456-64

    • Peer Reviewed
  • [Journal Article] Fluorescence cross-correlation analyses of the molecular interaction between an Aux/IAA protein, MSG2/IAA19, and protein-protein interaction domains of auxin response factors of arabidopsis expressed in HeLa cells2006

    • Author(s)
      Muto H, Nagao I, Demura T, Fukuda H, Kinjo M, Yamamoto KT
    • Journal Title

      Plant Cell Physiol 47(8)

      Pages: 1095-101

    • Peer Reviewed
  • [Journal Article] A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy2006

    • Author(s)
      Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A
    • Journal Title

      Nat Biotechnol 24(5)

      Pages: 577-81

    • Peer Reviewed
  • [Journal Article] Effect of cobalt on the liver glycogen content in the streptozotocin-induced diabetic rats2005

    • Author(s)
      Nomura Y, Okamoto S, Sakamoto M, Feng Z, Nakamura T
    • Journal Title

      Mol Cell Biochem 277(1-2)

      Pages: 127-130

    • Peer Reviewed
  • [Journal Article] Vanadate-induced exp-ression of hypoxia inducible factor-1α via oxygen-dependent and-independent pathways2005

    • Author(s)
      Nomura Y, Aragane A, Feng Z, Nakamura T.
    • Journal Title

      Biomed Res Trace Elem 16(4)

      Pages: 314-316

    • Peer Reviewed
  • [Journal Article] Genetic oxygen sensor : GFP as an indicatorof intracellular oxygenation2005

    • Author(s)
      Takahashi E, Takano T, Nomura Y, Okano S, Nakajima O, Sato M.
    • Journal Title

      Adv Exp Med Biol 566

      Pages: 39-44

    • Peer Reviewed
  • [Journal Article] An electro-tensile bioreactor for 3-D culturing of Cardiomyocytes2005

    • Author(s)
      Feng Z, Matsumoto T, Nomura Y, Kitajima T, Nakamura T
    • Journal Title

      IEEE Eng Med Biol 24(4)

      Pages: 73-79

    • Peer Reviewed
  • [Journal Article] Amphiphilic p-sulfonatocalix[4]arene-coated CdSe/ZnS quantum dots for the optical detection of the neurotransmitter acetylcholine2005

    • Author(s)
      Jin T, Fujii F, Sakata H, Tamura M, Kinjo M.
    • Journal Title

      Chem Commun(Camb) 14(34)

      Pages: 4300-2

    • Peer Reviewed
  • [Journal Article] Interaction of a small heat shock protein of the fission yeast, Schizosaccharomyces pombe, with a denatured protein at elevated temperature2005

    • Author(s)
      Hirose M, Tohda H, Giga-Hama Y, TsushimaR, Zako T, Iizuka R, Pack C, Kinjo M, Ishii N, Yohda M
    • Journal Title

      J Biol Chem 280(38)

      Pages: 32586-93

    • Peer Reviewed
  • [Journal Article] Dead-time distortion in fluorescence correlation measurements2005

    • Author(s)
      Nishimura G, Kinjo M.
    • Journal Title

      Appl Opt 44(17)

      Pages: 3458-67

    • Peer Reviewed
  • [Journal Article] Calixarene-coated water-soluble CdSe-ZnS semiconductor quantum dots that are highly fluorescent and stable in aqueous solution2005

    • Author(s)
      Jin T, Fujii F, Sakata H, Tamura M, Kinjo M.
    • Journal Title

      Chem Commun(Camb) 14(22)

      Pages: 2829-31

    • Peer Reviewed
  • [Journal Article] A new ultrasensitive way to circumvent PCR-based allele distinction : direct probing of unamplified genomic DNA by solution-phase hybridization using two-color fluorescence cross-correlation spectroscopy2005

    • Author(s)
      Foldes-Papp Z, Kinjo M, Tamura M, Birch-Hirschfeld E, Demel U, Tilz GP
    • Journal Title

      Exp Mol Pathol 78(3)

      Pages: 177-89

    • Peer Reviewed
  • [Journal Article] Analysis of cellular functions by multipoint fluorescence correlation spectroscopy2005

    • Author(s)
      Takahashi Y, Sawada R, Ishibashi K, MikuniS, Kinjo M.
    • Journal Title

      Curr Pharm Biotechnol 6(2)

      Pages: 159-65

    • Peer Reviewed
  • [Presentation] Matrix-targeted green fluorescent protein for quantitative monitoring of mitochondrial morphology in living cells2008

    • Author(s)
      Nomura Y, Takayama T, Sato M, Takahashi E, Feng Z, Nakamura T.
    • Organizer
      Experimental Biology
    • Place of Presentation
      San Diego
    • Year and Date
      2008-04-05
  • [Presentation] Effect of cobalt treatment on cell growth andmitochondrial DNA damage in HEK293 acutely exposed to hydrogen peroxide2006

    • Author(s)
      Nomura Y, Fujiwara H, Ito K, Sato M, Takahashi E, Hozumi Y, Feng Z, Nakamura T
    • Organizer
      Experimental Biology
    • Place of Presentation
      San Francisco
    • Year and Date
      20060401-05
  • [Presentation] Effect of cobalt on the liver glycogen content in the streptozotocin-induced diabetic rats2005

    • Author(s)
      Nomura Y, Okamoto S, Sakamoto M, Feng Z, Nakamura T
    • Organizer
      Experimental Biology
    • Place of Presentation
      San Diego
    • Year and Date
      20050402-06
  • [Book] ミトコンドリアレベルの酸素代謝測定からだと酸素の事典2009

    • Author(s)
      野村保友、Zhonggang Feng、中村孝夫
    • Publisher
      朝倉書店(印刷中)
  • [Book] DNA Damage Research(Nova Science Pub)2008

    • Author(s)
      Nomura Y, Feng Z, Nakamura T.
    • Total Pages
      1-8
    • Publisher
      Cobalt treatment suppressing mitochondrial DNA damage caused by acute exposure of H2O2
  • [Book] 蛋白質核酸酵素(細胞生物学における蛍光相関分光法と蛍光相互相関分光法蛋白質核酸酵素)(増刊号51(14))2006

    • Author(s)
      三國新太郎・金城政孝
    • Total Pages
      1998-2005
  • [Book] 磁界の生体に及ぼす影響2006

    • Author(s)
      中村孝夫、小野寿樹、野村保友、Zhonggang Feng
    • Total Pages
      25-34
    • Publisher
      山形医学

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Published: 2010-06-10   Modified: 2016-04-21  

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