Generation of Efficient Tailormade Biocatalysts (Catalytic Antibodies) by Heterologous Immuniztion

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 17510181
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Osaka Prefecture University
• Principal Investigator: TSUMURAYA Takeshi Osaka Prefecture University, Graduate School of Science, Associate Professor (00372855)
• Co-Investigator(Kenkyū-buntansha): FUJII Ikuo Osaka Prefecture University, Graduate School of Science (70189984)
• Project Period (FY): 2005 – 2007
• Keywords: catalytic antibody / Itransition-state analog / antigen / monoclonal antibody / hybridoma / immunization / directed evolution / library

Research Abstract

From the first report of catalytic antibodies in 1986, a variety of the antibodies has been generated and found to catalyze chemical transformations from peri-cyclic reactions to amido-bond hydrolyses. Most of them are elicited by immunization of transition-state analogs. However, unfortunately, the catalytic activities are not sufficient and the most of the reactions catalyzed by the antibodies are esterolytic hydrolysise. We have devised a new strategy called heterologous immunization wherein an animal is immunized in succession with two different but structurally related haptens. Upon heterologous immunization, the host may respond crossreactively to the secondary hapten and produce antibodies which have affinity for both of the primary and secondary hapten. When two haptens individually contain either a positive or negative charge incorporated in the structure of a transition-state analog, the heterologous immunization of this hapten pair provides an opportunity to simultaneously generate an acidic and an basic catalytic residue in an antibody combining site. Two successive immunization of phoshonate hapten, followed by a boost with amine hapten, induced catalytic antibodies with a higher rate acceleration compared with those obtained from immunization of a single hapten. The majority of these catalytic antibodies possessed cross-reactivities to both haptens, phoshonate and amine haptens, and the catalytic activities were effectively inhibited by both haptens. These results imply that the heterologous immunization strategy offers a potential means of introducing multiple catalytic residues into antibody combining site.

Research Products

• [Journal Article] Critical Contribution o fAromatic Rings to Specific Recognition of Polyether Ringsby Antibody:The Case of Ciguatoxin CTX3C and Its Specific Antibody IC9 (2008)
  • Author(s): Kouhei Tsumoto, Akiko Yokota, Yoshikazu Tanaka, Mihoko Ui, Takeshi Tsumuraya, Ikuo Fujii, Izumi Kumagai, Yoko Nagumo, Hiroki Oguri, Masayuki Inoue, and Masahiro Hirama
  • Journal Title: J.Biol.Chem 283 Pages: 12259-12266
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Selection of carbohydrate-binding domain with a helix-loop-helix structure (2008)
  • Author(s): Teruhiko Matsubara, Mie Iida, Takeshi Tsumuraya, Iuko Fujii, and Toshinori Sato
  • Journal Title: Biochemistry 47
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] How protein recognizes ladder-like polycyclic ethers:interactions between ciguatoxin (CTX3C) fragments and its specific antibody 10C9 (2008)
  • Author(s): Mihoko Ui, Yoshikazu Tanaka, Takeshi Tsumuraya, Ikuo Fujii, Masayuki Inoue, Masahiro Hirama, Kouhei Tsumoto
  • Journal Title: J.Biol.Chem 283
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular Basis for Transition-state Stabilization in Catalytic Antibodies (2008)
  • Author(s): Takeshi Tsumuraya and Ikuo Fujii
  • Journal Title: Bulletin of the Chemical Society of Japan 81
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Isolation of IL 5 Receptor Interacting Peptides Using a Constrained Helical Peptide Phage Display Library (2008)
  • Author(s): Zengmao, Ye, Takeshi, Tsumuraya, Yuko, Ishmo, Yukiko, Nishida, Ikuo, Fujii
  • Journal Title: Peptide Science Pages: 391-392
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Critical Contribution of Aromatic Rings to Specific Reconition of Polyether Rings by Antibody The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C9 (2008)
  • Author(s): Kouhei, Tsumoto, Akiko, Yoshida, Yoshikazu, Tanaka, Mihoko, Ui, Takeshi, Tsumuraya, Ikuo, Fujn, Izumi, Kumagai, Yoko, Nagumo, Hiroki, Oguri, Masayuki, Inoue, Masalmo, Hirama
  • Journal Title: J Biol Chem 283 Pages: 12259-12266
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Selection of caibohydrate binding domain with a helix-loop helix structure (2008)
  • Author(s): Teruhiko, Matsubara, Mie, Iida, Takeshi, Tsumuraya, Ikuo, Fujii, Toshinori, Sato
  • Journal Title: Biochemistry in press
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] How protein recognizes ladder-like polycyclic ethers Interactions between ciguatoxin (CTX3C) fragments and its specific antibody 10C9 (2008)
  • Author(s): Mihoho, Ui, Yoshikazu, Tanaka, Takeshi, Tsumuraya, Ikuo, Fuju, Masayuki, Inoue, Takeshi, Tsumuraya
  • Journal Title: J Biol Chem in press
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular Basis for Transition state Stabilization in Catalytic Antibodies (2008)
  • Author(s): Takeshi, Tsumuraya, Ikuo Fuju
  • Journal Title: Bulletin of the Chmical Society in press
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Thermodynamic and Structural Bases for Transition-state stabilization in an Antibodycatalyzed Hydrolysis (2007)
  • Author(s): Masayuki Oda, Nobutoshi Ito, Takeshi Tsumuraya, Kayo Suzuki, Masayoshi Sakakura, Ikuo Fuju
  • Journal Title: J.Mol.Biol 369 Pages: 198-209
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Isolation of IL-5 Receptor Interacting Peptides Using a Constrained Helical Peptide Phage Display Library (2007)
  • Author(s): Zhengmao Ye, Takeshi Tsumuraya, Yuko Ishino, Yukiko Nishida, and Ikuo Fujii
  • Journal Title: Peptide Science Pages: 391-392
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Thermodynamic and Structural Bases for Transition state stabilization in an Antibody catalyzed Hydrolysis (2007)
  • Author(s): Masayuki, Oda, Notutoshi, Ito, Takeshi, Tsumuiaya, Kayo, Suzuki, Masayoshi, Sakakura, Ikuo, Finn
  • Journal Title: J Mol Biol 369 Pages: 198-209
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] コンビナトリアル・バイオエンジニアリングによるテーラーメイド機能性抗体の創製 (2006)
  • Author(s): 円谷 健, 藤井 郁雄
  • Journal Title: 生物工学会誌 84 Pages: 316-318
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] テーラーメイド生体触媒(抗体酵素)の分子設計 (2006)
  • Author(s): 円谷 健, 藤井 郁雄
  • Journal Title: 酵素工学ニュース 56 Pages: 30-36
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular Mechanisms of Transition-state Stabilization in Catalytic Antibodies (2006)
  • Author(s): Takeshi Tsumuraya, Ikuo Fujii
  • Journal Title: J.Synth.Org.Chem.Jpn 64 Pages: 1159-1170
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Production of monoclonal antibodies for sandwich immunoassay detection of 51-hydroxyCTX3C (2006)
  • Author(s): Takeshi Tsumuraya, Ikuo Fujii, Masayuki Inoue, Atsushi Tatami, Keisuke Miyazaki, and Masahiro Hirama
  • Journal Title: Toxicon 48 Pages: 287-294
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Luciferase Antibody (2006)
  • Author(s): Takeshi Tsumuraya, Chun Wu, Hideshi Nakamura, Satoshi Inouye, and Ikuo Fujii
  • Journal Title: Peptide Science Pages: 128-129
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A Phage Displayed Library of Constrained Loop Peptides (2006)
  • Author(s): Daisuke Fujiwara, Zengmao Ye, Takeshi Tsumuraya, and Ikuo Fujii
  • Journal Title: Peptide Science Pages: 336
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Screening of Peptide Antagonistsfbr Cytokine Receptors from Phage-Displayed Libraries (2006)
  • Author(s): Zhengmao Ye, Takeshi Tsumuraya, Daisuke Fujiwara, and Ikuo Fujii
  • Journal Title: Peptide Science Pages: 337
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Generateion of Tailor-made Biocatalysts by Combinatorial Bioengineering (2006)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Fujn
  • Journal Title: Seibutsu-Kougaku Kaishi 84 Pages: 316-318
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Moleculai Design of Tailor made Biocatalysts(Cataltyci Antibodies) (2006)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Fujii
  • Journal Title: Enzyme Engineering News 56 Pages: 30-36
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular Mechanism of Transition state Stabilization in Catalytic Antibodies (2006)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Finn
  • Journal Title: J Synth Org Chem , Jpn 64 Pages: 1159-1170
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Production of monoclonal antibodies foi sandwich immunoassay detection of 51-hydroxyCTX3C (2006)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Fujn, Masayuki, Inoue, Atsushi, Tatami, Keisuke, Miyazaki, Masahiro, Hirama
  • Journal Title: Toxicon 48 Pages: 287-294
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 「研究成果報告書概要(欧文)」より (2006)
  • Author(s): Takeshi, Tsumuraya, Chun, Wu, Hideshi, Nakamura, Satoshi, Inoue, Ikuo, Fujii, Luciferase, Antibody
  • Journal Title: Peptide Science Pages: 128-129
• [Journal Article] A Phage Displayed Library of Constrained Loop Peptide (2006)
  • Author(s): Daisuke, Fujiwara, Zengmao, Ye, Takeshi, Tsumuraya, Ikuo Fujii
  • Journal Title: Peptide Science 336
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Screening of Peptide Antagonists for Cytokine Receptors from Phage-Displayed Libraries (2006)
  • Author(s): Zengmao, Ye, Takeshi, Tsumuraya, Daisuke, Fujiwara, Ikuo, Fuju
  • Journal Title: Peptide Science 337
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 触媒機能を有する抗体タンパク質 (2005)
  • Author(s): 円谷 健
  • Journal Title: 化学と教育 53 Pages: 440-443
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Antibodies with Enzymatic Activities (2005)
  • Author(s): Takeshi, Tsumuraya
  • Journal Title: Chemistry and Education 53 Pages: 440-443
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ポロ抗体酵素 (2008)
  • Author(s): 円谷 健, 石川 文洋, 藤井 郁雄
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Holoabzyme (2008)
  • Author(s): Takeshi, Tsumuraya, Fumihiro, Ishikawa, Ikuo, Fujii
  • Organizer: 88th Annual Meeting of the Chemical Society of Japan
  • Place of Presentation: Tokyo
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Analyses of Luciferase Antibodies by Yiest Surface Display (2008)
  • Author(s): Takaaki, Kojima, Atsuko, Uyeda, Fumihiro, Ishikawa, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: 2008 Annual Meeting of Japan Society for Bioscience, Biotechnology, and Agrochemistry
  • Place of Presentation: Nagoya
  • Year and Date: 2008-03-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 酵母表層ディスプレイ法を用いたルシフェラーゼ抗体酵素の解析 (2008)
  • Author(s): 児島 孝明, 植田 敦子, 石川 文洋, 円谷 健, 藤井 郁雄
  • Organizer: 2008年農芸化学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ポロ抗体酵素(holoabzyme):人工補酵素導入型抗体によるアルドール反応 (2008)
  • Author(s): 石川 文洋, 円谷 健, 藤井 郁雄
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Holoabzyme: Aldol Reactions Catalyzed by Cofactor-mediated Abzvme (2008)
  • Author(s): Fumihiro, Ishikawa, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: 128th Annual Meeting of the Pharmaceutical Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 抗シガトキシン抗体1C49のポリエーテル環の特異的認識における芳香族環の影響 (2008)
  • Author(s): 田中 良和, 津本 浩平, 横田 亜紀子, 宇井 美穂子, 円谷 健, 藤井 郁雄, 熊谷 泉, 南雲 陽子, 大栗 博毅, 井上 将行, 平間 正博
  • Organizer: SORST ジョイントシンポジウム (8)
  • Place of Presentation: 東京
  • Year and Date: 2008-01-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 抗シガトキシン抗体による合成環状エーテル抗原の認識機構 (2008)
  • Author(s): 宇井 美穂子, 田中 良和, 円谷 健, 藤井 郁雄, 井上 将行, 平間 正博, 津本 浩平
  • Organizer: SORST ジョイントシンポジウム (8)
  • Place of Presentation: 東京
  • Year and Date: 2008-01-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 抗太平洋型シガトキシン抗体の調製とサンドイッチイムノアッセイによるシガトキシンの検出 (2008)
  • Author(s): 藤井 郁雄, 円谷 健, 北川 大輔, 井上 将行, 平間 正博
  • Organizer: SORST ジョイントシンポジウム (8)
  • Place of Presentation: 東京
  • Year and Date: 2008-01-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 抗カリブ海型シガトキシン抗体の調製 (2008)
  • Author(s): 円谷 健, 斉藤 雄一郎, 北川 大輔, 井上 将行, 平間 正博, 藤井 郁雄
  • Organizer: SORST ジョイントシンポジウム (8)
  • Place of Presentation: 東京
  • Year and Date: 2008-01-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecular Recognition of cyclic Poly Ethers in anti-Ciatiatoxin Antibody (2008)
  • Author(s): Mihoko, Ui, Yoshikazu, Tanaka, Takeshi, Tsumuraya, Ikuo, Fujii, Masayuki, Inoue, Masahiro, Hirama, Kouhei, Tsumoto
  • Organizer: SORST Joint Symposium (8)
  • Place of Presentation: Tokyo
  • Year and Date: 2008-01-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Generation of anti-Ciguatoxin Antibodies and Detection of Ciguatoxins by Sandwich Immunoassay (2008)
  • Author(s): Ikuo, Fujii, Takeshi, Tsumuraya, Daisuke, Kitagawa, Masayuki, Inoue, Masahiro, Hirama
  • Organizer: SORST Joint Symposium (8)
  • Place of Presentation: Tokyo
  • Year and Date: 2008-01-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Generation of anti-Carribean Ciguatoxin Antibodies (2008)
  • Author(s): Takeshi, Tsumuraya, Yuichiro, Saito, Daisuke, Kitagawa, Masayuki, Inoue, Masahiro, Hirma, Ikuo, Fujii
  • Organizer: SORST Joint Symposium (8)
  • Place of Presentation: Tokyo
  • Year and Date: 2008-01-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ポロ抗体酵素 (2008)
  • Author(s): 円谷 健, 石川 文洋, 藤井 郁雄
  • Organizer: 第10回生命化学研究会シンポジウム
  • Place of Presentation: 熊本
  • Year and Date: 2008-01-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Holoabzyme (2008)
  • Author(s): Takeshi, Tsumuraya, Fumihiro, Ishikawa, Ikuo, Fujii
  • Organizer: 10th Symposium of the Forum on Biomolecular Chemistry
  • Place of Presentation: Kumamoto
  • Year and Date: 2008-01-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of Aromatic Residues on the Specific Recognition of anti-Ciguatoxin Antibody 1C49 (2008)
  • Author(s): Yoshikazu, Tanaka, Kouhei, Tsurrioto, Akiko, Yokota, Mihoko, Ui, Takeshi, Tsumuraya, Ikuo, Fujii, Izumi, Kumagai, Yoko, Nagumo, Hiroki, Oguri, Masayuki, Inoue, Masahiro, Hirama
  • Organizer: SORST Joint Symposium (8)
  • Place of Presentation: Tokyo
  • Year and Date: 2008-01-08
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Luciferase Antibody (2007)
  • Author(s): Takeshi Tsumuraya, Chun Wu, Hideshi Nakamura, Satoshi Inouye, and Ikuo Fujii
  • Organizer: International Conference of 43_<rd> Japanese Peptide Symposiumand 4_<th> Peptide Engineering Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2007-11-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Peptide Antagonists for Cytokine Receptors from Phage-displayed Libraries (2007)
  • Author(s): Zengmao Ye, Takeshi Tsumuraya Daisuke Fujiwara, and Ikuo Fujii
  • Organizer: International Conference of 43_<rd> Japanese Peptide Symposiumand 4_<th> Peptide Engineering Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2007-11-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 立体構造規制ペプチドライブラリー:IL5受容体親和性ペプチドの獲得 (2007)
  • Author(s): 叶正 茂, 円谷 健, 石野 祐子, 西田 友貴子, 藤井 郁雄
  • Organizer: 第44回ペプチド討論会
  • Place of Presentation: 富山
  • Year and Date: 2007-11-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Constrained Peptide Library: Generation of IL-5 Receptor specific Peptides (2007)
  • Author(s): Zengmao, Ye, Takeshi, Tsumuraya, Yuko, Ishino, Yukiko, Nishida, Ikuo, Fujii
  • Organizer: 44th Japan Peptide Symposium
  • Place of Presentation: Toyama
  • Year and Date: 2007-11-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 合成ハプテンを用いた抗シガトキシン抗体の作製とサンドイッチイムノアッセイ法の開発 (2007)
  • Author(s): 円谷 健, 藤井 郁雄, 大栗 博毅, 上原 久俊, 丸山 潤美, 新藤 由美, 佐々木 信也, 平間 正博
  • Organizer: 第33回反応と合成の進歩シンポジウム
  • Place of Presentation: 長崎
  • Year and Date: 2007-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Synthesis-based Approach Toward Direct Sandwich Immunoassay for Ciguatoxins (2007)
  • Author(s): Takeshi, Tsumurya, Ikuo, Fujii, Hiroki, Oguri, Hisat,oshi, Uehara, Megumi, Maruyama, Yumi, Shindo, Shinya, Sssaki, Masahiro, Hirama
  • Organizer: 33rd Symposium on Progress In Organic Reactions and Synthesis
  • Place of Presentation: Nagasaki
  • Year and Date: 2007-11-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ポロ抗体酵素 (2007)
  • Author(s): 石川 文洋, 円谷 健, 藤井 郁雄
  • Organizer: 酵素工学研究会第58講演会
  • Place of Presentation: 東京
  • Year and Date: 2007-10-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Holoabzyme (2007)
  • Author(s): Fumihiro, Ishikawa, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: 58th Symosium of Japan Society of Enzyme Engineering
  • Place of Presentation: Tokyo
  • Year and Date: 2007-10-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] コンポジット型触媒抗体 (2007)
  • Author(s): 石川 文洋, 円谷 健, 藤井 郁雄
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 抗シガトキシン抗体の分子認識:結晶構造と熱力学 (2007)
  • Author(s): 宇井 美穂子, 田中 良和, 円谷 健, 藤井 郁雄, 井上 将行, 平間 正博, 津本 浩平
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Composite Catalytic Antibodies (2007)
  • Author(s): Fumihiro, Ishikawa, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: 20th Symposium on Biofunctional Chemistry
  • Place of Presentation: Sendai
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Molecular Recognition Analyses of anti-Ciguatoxin Antibodies, Crystal Structure and Thermodynamic Analyses (2007)
  • Author(s): Mihoko, Ui, Yoshikazu, Tanaka, Takeshi, Tsumuraya, Ikuo, Fujii, Masayuki, Inoue, Masahiro, Hirama, Kouhei, Tsumtoto
  • Organizer: 20th Symposium on Biofunctional Chemistry
  • Place of Presentation: Sendai
  • Year and Date: 2007-09-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] SCREENING OF PEPTIDE INHIBITORES FOR AURORA KINASES FROM A DE NOVO DESIGNED α-HELICAL PEPTIDE PHAGE・DISPLAY LIBRARY (2007)
  • Author(s): Takeshi Tsumuraya, Daisuke Fujiwara, and Ikuo Fujii
  • Organizer: The2_<nd> International Workshop on Approaches to Single-Cell Analysis
  • Place of Presentation: 東京
  • Year and Date: 2007-09-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] SCREENING OF PETIDE INHIBITORS FOR AURORA KINASES FROM A DE NOVO DESIGNED a HELICAL PEPTIDE PHAGE DISPLAYED LIBRARY (2007)
  • Author(s): Takeshi, Tsumuraya, Daisuke, Fujiwara, Ikuo, Fujii
  • Organizer: The 2nd International Workshop on Approaches to Single Cell Analysis
  • Place of Presentation: Tokyo
  • Year and Date: 2007-09-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 立体構造規制ペプチドライブラリー:IL5受容体親和性ペプチドの獲得 (2007)
  • Author(s): 円谷 健, 呉 純, 中村 英士, 井上 敏, 藤井 郁, 円谷 健, 叶正 茂, 石野 祐子, 西田 友貴子, 藤井 郁雄
  • Organizer: 第10回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] X線結晶構造解析による抗シガトキシン抗体の分子認識機構の解明 (2007)
  • Author(s): 宇井 美穂子, 田中 良和, 円谷 健, 藤井 郁雄, 井上 将行, 平間 正博, 津本 浩平
  • Organizer: 第10回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Constrained Peptide Library IL 5 Receptor Specific Peptides (2007)
  • Author(s): Takeshi, Tsumuraya, Ye, Zengmao, Yuko, Ishmo, Yukiko, Nishida, Ikuo, Fujii
  • Organizer: 10th Symposium of Division of Biotechnology
  • Place of Presentation: Tokyo
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Study of Molecular Recognition of and Ciguatoxin Antibody by X-ray Crystal Analysis (2007)
  • Author(s): Mihoko, Ui, Yoshikazu, Tanaka, Takeshi, Tsumuraya, Ikuo, Fuju, Masayuki, Inoue, Masahiro, Hirama, Kouhei, Tsumoto
  • Organizer: 10th Symposium of Division of Biotechnology
  • Place of Presentation: Tokyo
  • Year and Date: 2007-09-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 単一の遷移状態アナログより誘導される抗体酵素の触媒機構 (2007)
  • Author(s): 円谷 健
  • Organizer: 第1回生体分子科学シンポジウム「抗体酵素の構造と機能」
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Molecula, Mechanism of Antibodies Elicited against a Single Transition-state Analog (2007)
  • Author(s): Takeshi, Tsumuraya
  • Organizer: 1st Biomolecular Symposium
  • Place of Presentation: Osaka
  • Year and Date: 2007-03-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] コファクター分子を抗原結合部位にもつコンポジット型触媒抗体 (2007)
  • Author(s): 石川 文洋, 円谷 健, 藤井 郁雄
  • Organizer: 第2回産業用酵素シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ルシフェラーゼ抗体酵素 (2007)
  • Author(s): 円谷 健, 呉 純, 中村 英士, 井上 敏, 藤井 郁雄
  • Organizer: 第2回産業用酵素シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Composit Catalytic Antibodies for Cofactor mediated Transformations (2007)
  • Author(s): Fumihiro, Ishikawa, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: 2nd Symposium on Advance Enzyme Technology
  • Place of Presentation: Osaka
  • Year and Date: 2007-03-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Luciferase Antibody Catalysts (2007)
  • Author(s): Takeshi, Tsumuraya, Chun, Wu, Hideshi, Nakamura, Satoshi, Inouye, Ikuo, Fuui
  • Organizer: 2nd Symposium on Advance Enzyme Technology
  • Place of Presentation: Osaka
  • Year and Date: 2007-03-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 抗シガトキシン抗体の作製と微量検出法の開発 (2007)
  • Author(s): 藤井 郁雄, 円谷 健, 北川 大輔
  • Organizer: 平成18年度戦力的創造研究(SORST)ミニシンポジウム
  • Place of Presentation: 沖縄
  • Year and Date: 2007-03-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Generation of anti-Ciguatoxin Antibodies and Development of Detection Method for Ciguatoxins (2007)
  • Author(s): Ikuo, Fujii, Takeshi, Tsumuraya, Daisuke, Kitagawa
  • Organizer: SORST Mini Symposium
  • Place of Presentation: Okinawa
  • Year and Date: 2007-03-09
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Thermodynamic and Structural Analyses of Hydrolytic Mechanismby Catalytic Antibodies (2006)
  • Author(s): Takeshi Tsumuraya, Masayuki Oda, Nobutoshi Ito, Kayo Suzuki, Ikuo Fujii
  • Organizer: Japan-ltaly Symposium of New Trendsin Enzyme Science and Technology
  • Place of Presentation: Nagoya
  • Year and Date: 2006-11-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Antibody Catalysis for Cofactormediated Chemical Transformations (2006)
  • Author(s): Fumihiro Ishikawa, Takeshi Tsumuraya, and Ikuo Fujii
  • Organizer: Japan-ltaly Symposium of New Trendsin Enzyme Science and Technology
  • Place of Presentation: Nagoya
  • Year and Date: 2006-11-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] A Phage Displayed Library of Constrained Loop Peptides (2006)
  • Author(s): Daisuke Fujiwara, Zengmao Ye, Takeshi Tsumuraya, and Ikuo Fujii
  • Organizer: International Conference of 43_<rd> Japanese Peptide Symposiumand 4_<th> Peptide Engineering Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2006-11-06
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Luciferase Antibody (2006)
  • Author(s): Takeshi, Tsumuraya, Chun, Wu, Hideshi, Nakamura, Satoshi, Inouye, Ikuo, Fujii
  • Organizer: International Conference of 43rd Japanese Peptide Symposium and 4th Peptide Engineerign Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2006-11-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Peptide Antagonists for Cytokine Receptors from Phage displayed Libraries (2006)
  • Author(s): Zengmao, Ye, Takeshi, Tsumuraya, Daisuke, Fujiwara, Ikuo, Fuju
  • Organizer: International Conference of 43rd Japanese Peptide Symposium and 4th Peptide Engineerign Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2006-11-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] A Phage Displayed Library of Constrained Loop Peptides (2006)
  • Author(s): Daisuke, Fujiwara, Zengmao, Ye, Takeshi, Tsumuraya, Ikuo, Fuju
  • Organizer: International Conference of 43,il Japanese Peptide Symposium and 4,th Peptide Engineerign Meeting
  • Place of Presentation: Yokohama
  • Year and Date: 2006-11-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Thermodynamic and Structural Analyses of Hydrolytic Mechanism by Catalvtir Antibodies (2006)
  • Author(s): Takeshi, Tsumuraya, Masayuki, Oda, Nobutoshi, Ito, Kayo, Suzuki, Ikuo, Fujii
  • Organizer: Japan-Italy Symposium of New Trends in Enzyme Science and Technology
  • Place of Presentation: Nagoya
  • Year and Date: 2006-11-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Antibody Catalysis for Cofacotr mediated Transformations (2006)
  • Author(s): Fumihiro, Ishikawa, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: Japan Italy Symposium of New Trends in Enzyme Science and Technology
  • Place of Presentation: Nagoya
  • Year and Date: 2006-11-06
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Luciferase antibody, Takeshi Tsumuraya, Chun Wu ,Hideshi Nakamura, Satoshi Inouye, and Ikuo Fuju (2006)
  • Author(s): Takeshi Tsumuraya, Chun Wu, Hideshi Nakamura, Satoshi Inouye, and Ikuo Fujii
  • Organizer: 中韓酵素工学会議
  • Place of Presentation: 滋賀
  • Year and Date: 2006-10-31
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Luciferase Antibody (2006)
  • Author(s): Takeshi, Tsumuraya, Chun, Wu, Hideshi, Nakamura, Satoshi, Inouye, Ikuo, Fuju
  • Organizer: Japan China Korea Joint Sympsium on Enzyme Engineering
  • Place of Presentation: Shiga
  • Year and Date: 2006-10-31
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Composit Catalytic Antibodies having a Cofactor in the Antibody binding Site (2006)
  • Author(s): Fujihiro, Ishikawa, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: Joint Symposium on Biorelevant Chemistry
  • Place of Presentation: Kyoto
  • Year and Date: 2006-09-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ファクター分子を抗原結合部位にもつコンポジソト型触媒抗体 (2006)
  • Author(s): 石川 文洋, 円谷 健, 藤井 郁雄
  • Organizer: 第21回生体機能関連化学部会, 第9回バイオテクノロジー部会, 第9回生命化学研究会合同シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2006-09-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Helix loop helix Pepide Libraries Molecular Evolution of Peptide Ligands for G. CSF Receptor (2006)
  • Author(s): Takeshi, Tsumuraya, Yasushi, Zenitani, Yumiko, Mizukoshi, Akiko, Matsui, Zengmao, Ye, Ikuo, Fujn
  • Organizer: Joint Symposium on Biorelevant Chemistry
  • Place of Presentation: Kyoto
  • Year and Date: 2006-09-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] テーラーメイド機能性抗体の創製 (2006)
  • Author(s): 円谷 健
  • Organizer: 日本生物高分子学会2006年度大会
  • Place of Presentation: 京都
  • Year and Date: 2006-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ヘリソクス・ループ・ヘリックズペプチドライブラリー:G・CSF受容体結合性ペプチドの構造安定性および親和性の向上 (2006)
  • Author(s): 円谷 健, 銭谷 康志, 水越 弓子, 松居 明子, 叶正 茂, 藤井 郁雄
  • Organizer: 第21回生体機能関連化学部会, 第9回バイオテクノロジー部会, 第9回生命化学研究会合同シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2006-09-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Generation of Taior-made Biocatalysis (2006)
  • Author(s): Takeshi, Tsumuraya
  • Organizer: Annual Meeting of Japan Science Society of Biological Macromolecules
  • Place of Presentation: Kyoto
  • Year and Date: 2006-09-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Thermodynamic and Strucural Analyses o fHydrolytic Mechanismby Catalytic Antibodies (2006)
  • Author(s): T.Tsumuraya, M.Oda, N.Ito, K.Suzuki, and I.Fujii
  • Organizer: The 20_<th> Symposium of the Protein Society
  • Place of Presentation: SanDiego,USA
  • Year and Date: 2006-08-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Thermodynamic and Structural Analyses of Hydrolytic Mechanism by Catalytic Antibodies (2006)
  • Author(s): T, Tsumuraya, M, Oda, N, Ito, K, Suzuki, I, Fuju
  • Organizer: The 20th Symposium of the Protein Society
  • Place of Presentation: SanDiego, USA
  • Year and Date: 2006-08-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 抗シガトキシンおよびサイトカイン受容体結合ペプチド (2006)
  • Author(s): 円谷 健
  • Organizer: バイオ EXPO
  • Place of Presentation: 東京
  • Year and Date: 2006-06-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Generation of and Ciguatoxin Antibodies and Citokine Specific Peptides (2006)
  • Author(s): Takeshi, Tsumuraya
  • Organizer: Bio EXPO Japan
  • Place of Presentation: Tokyo
  • Year and Date: 2006-06-01
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Snthesis based Aproach toward Direct Sandwich Imunoassav for Ciguatoxins (2006)
  • Author(s): Takeshi, Tsumuraya, Hiroki, Oguri, Naomasa, Oshiro, Toshiki, Sasaki, Masayuki, Inoue, Masahiro, Hirama, Ikuo, Fujii
  • Organizer: 86th Annual Meeting of the Chemical Society of Japan
  • Place of Presentation: Funabashi
  • Year and Date: 2006-05-30
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 合成ハプテンを用いた抗シガトキシン抗体の作製:サンドイッチイムノアッセイ法の開発と分子認識 (2006)
  • Author(s): 円谷 健, 大栗 博毅, 佐藤 威, 大城 直雅, 佐々木 俊樹, 井上 将行, 平間 正博, 藤井 郁雄
  • Organizer: 日本化学会第86春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2006-03-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 合成ハプテンを用いた抗シガトキシン抗体の作製:サンドイッチイムノアッセイ法の開発と分子認識 (2006)
  • Author(s): 円谷 健, 大栗 博毅, 佐藤 威, 大城 直雅, 佐々木 俊樹, 井上 将行, 平間 正博, 藤井 郁雄
  • Organizer: 第8回生命化学研究会シンポジウムin富山(2006)
  • Place of Presentation: 富山
  • Year and Date: 2006-01-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Snthesis based Aproach toward Direct Sandwich Imunoassay for Ciguatoxins (2006)
  • Author(s): Takeshi, Tsumuraya, Hiroki, Oguri, Naomasa, Oshiro, Toshiki, Sasaki, Masayuki, Inoue, Masahiro, Hirama, Ikuo, Fuju
  • Organizer: 8th Symposium of the Forum on Biomolecular Chemistry
  • Place of Presentation: Toyama
  • Year and Date: 2006-01-13
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] コンビナトリアル・バイオエンジニアリングによるテーラーメイド機能性抗体の創製 (2006)
  • Author(s): 円谷 健, 藤井 郁雄
  • Organizer: ナノ・バイオとコンビナトリアル・バイオのジョイントシンポジウム:第6回コンビナトリアル・バイオエンジニアリングシンポジウム-新バイオ分子と機能の創出を目指して
  • Place of Presentation: 東京
  • Year and Date: 2006-01-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Generation of Tailor made Biocatalysts by Combinatorial Bioengineering (2006)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Fujn
  • Organizer: 5th Symposium on Combinatorial Bioengineering
  • Place of Presentation: Tokyo
  • Year and Date: 2006-01-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] SYNTHESIS-BASED APPROACH TOWARD DIRECT SANDWICH IMMUNOASSAY FOR CIGUATOXINS (2005)
  • Author(s): Takeshi Tsumuraya, Hiroki Oguri, Takeshi Satoh, Naomasa Oshiro, Sasaki Toshiki, Masayuki Inoue, Ikuo Fujii, Masahiro, Hirama
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu,USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] TWO DISTINCT STRUCTURES OF CATALYTIC ANTIBODIES GENERATED AGAINST A SINGLE TRANSITION-STATE ANALOG (2005)
  • Author(s): Takeshi Tsumuraya, Ikuo Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu,USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] DIRECTED EVOLUTION OF ANTIBODY CATALYSTS (2005)
  • Author(s): Takeshi Tsumuraya, Ikuo Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu,USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Screening of Antagonist Peptides of Cytokine Receptors from a Denovo Desinged a-Helix Peptide Phage-display Library (2005)
  • Author(s): Zengmao Ye, Takesh Tsumuraya, Toshinaga Tsuji, Ikuo Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu,USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Antibody Catalysts for Co-factor Mediated Chemical Transfomations (2005)
  • Author(s): Fumihiro Ishikawa, Takeshi Tsumuraya, Ikuo Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu,USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Downsizing of Helix-loop-helix Peptides binding for G-CSF Receptor (2005)
  • Author(s): Kaori Yamatogi, Takeshi Tsumuraya, Kazuhisa Fujimoto, Mitsunori Kirihata, Ikuo Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu,USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The Kinetic and Sequence Analyses of Luciferase Antibodies (2005)
  • Author(s): Tatsuya Mineoka, Takeshi Tsumuraya, Ikuo Fuju
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu,USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] SYNTHESIS-BASED APPROACH TOWARD DIRECT SANDWICH EMMUNOASSAY FOR CIGUATOXINS (2005)
  • Author(s): Takeshi, Tsumuraya, Hiroki, Oguri, Takeshi, Satoh, Naomasa, Oshiro, Toshiki, Sasaki, Masayuki, Inoue, Ikuo, Fuju, Masahiro, Hirama
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] TWO DISTINCT STRUCTURES OF CATALYTIC ANTIBODIES GENERATED AGAINST A SINGLE TRANSITION STATE ANALOG (2005)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Funi
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] DIRECTED EVOLUTION OF ANTIBODY CATALYSTS (2005)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Screening of Antagonist Peptides of Cytokine Receptors from a De Novo Designed a Helix Peptide Phage display Library (2005)
  • Author(s): Zengmao, Ye, Takeshi, Tsumuraya, Toshinaga, Tsuji, Ikuo, Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, IJSA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Catalysts for Co factor Mediated Chemical Transformations (2005)
  • Author(s): Fumihno, Ishikawa, Takeshi, Tsumuraya, Ikuo, Antibody
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Downsizing of Helix-loop-helix Peptides binding for G CSF Receptor (2005)
  • Author(s): Kaori, Yamatogi, Takeshi, Tsumuiaya, Kazuhisa, Fujimoto, Mitsunori, Kirihata, Ikuo, Fujn
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The Kinetic and Sequence Analyses of Luciferase Antibodies (2005)
  • Author(s): Tatsuya, Mmeoka, Takeshi, Tsumuraya, Ikuo, Fujii
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2005-12-17
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] テーラーメイド機能性抗体の創製と分子認識 (2005)
  • Author(s): 円谷 健
  • Organizer: SORST ジョイントシンポジウム (4)
  • Place of Presentation: 大阪
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] シガトキシン類縁体の微量検出法の確立 (2005)
  • Author(s): 北川 大輔, 円谷 健, 藤井 郁雄, 平間 正博
  • Organizer: SORST ジョイントシンポジウム (4)
  • Place of Presentation: 大阪
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 一般酸・塩基触媒や求核触媒機能を抗原結合部位に導入するための方法論の開発と高活性抗体の取得」 (2005)
  • Author(s): 石川 文洋, 円谷 健, 藤井 郁雄
  • Organizer: SORST ジョイントシンポジウム (4)
  • Place of Presentation: 大阪
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 顆粒球コロニー刺激因子レセプター結合性ペプチドの低分子化 (2005)
  • Author(s): 山磨 香織, 円谷 健, 藤本 和久, 藤井 郁雄
  • Organizer: SORST ジョイントシンポジウム (4)
  • Place of Presentation: 大阪
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ルシフェラーゼ活性を持つ抗体触媒 (2005)
  • Author(s): 峯岡 竜也, 円谷 健, 藤井 郁雄
  • Organizer: SORST ジョイントシンポジウム (4)
  • Place of Presentation: 大阪
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Generation and Molecular Recognition of Tailor made Biocatalysts (2005)
  • Author(s): Takeshi, Tsumuraya
  • Organizer: SORST Joint Symposium(4)
  • Place of Presentation: Osaka
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Detections of Ciguatoxin Congeners (2005)
  • Author(s): Daisuke, Kitagawa, Takeshi, Tsumuraya, Ikuo, Fujii, Masahiro, Hirama
  • Organizer: SORST Joint Symposium(4)
  • Place of Presentation: Osaka
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Generation of Catalytic Antibodies having general acid-base and nucleonhilic catalysts (2005)
  • Author(s): Fumihiro, Ishikawa, Takeshi, Tsumurya, Ikuo, Fujn
  • Organizer: SORST Joint Symposium(4)
  • Place of Presentation: Osaka
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Downsizing of a paptide binding specifically with G CSF receptor (2005)
  • Author(s): Kaori, Yamatogi, Takeshi, Tsumuraya, Kazuhisa, Fujimoto, Ikuo, Fujn
  • Organizer: SORST Joint Symposium(4)
  • Place of Presentation: Osaka
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Luciferase Antibody (2005)
  • Author(s): Tatsuya, Mineoka, Takeshi, Tsumuraya, Ikuo, Fujn
  • Organizer: SORST Joint Symposium(4)
  • Place of Presentation: Osaka
  • Year and Date: 2005-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Directed evolution o fcatalytic antibodies inphage-displayed combinatorial libraries (2005)
  • Author(s): Takeshi Tsumuraya, Ikuo Fujii
  • Organizer: Enzyme Engineering XVIII Conference
  • Place of Presentation: Gyeong-ju,Korea
  • Year and Date: 2005-10-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Directed evlolition of catalytic antibodies in phage displayed combinatoiial libraiie (2005)
  • Author(s): Takeshi, Tsumuraya, Ikuo, Fujn
  • Organizer: Enzyme Engineering XVIII Conference
  • Place of Presentation: Gyeone-ju. Korea
  • Year and Date: 2005-10-09
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] α-ヘリックス・ペプチドライブラリーの分子設計:G-CSF受容体結合ペプチドの探索と分子進化 (2005)
  • Author(s): 藤井 郁雄, 水越 弓子, 銭谷 康志, 松居 明子, 山磨 香織, 叶正 茂, 円谷 健
  • Organizer: 第20回生体機能関連化学シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2005-09-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] De novo Design of a Helical Peptide Libraries Dnected Evolution of Peptide Ligands foi G-CSF Receptor (2005)
  • Author(s): I. Fujii, Y. Mizukoshi, Y. Zenitani, A. Matsui, K. Yamatogi, Z. Ye, T. Tsumuraya
  • Organizer: 20th Symposium on Biofunctional Chemistry
  • Place of Presentation: Nagoya
  • Year and Date: 2005-09-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 合成ハプテンを用いた抗シガトキシン抗体の作製とサンドイッチイムノアッセイ法の開発 (2005)
  • Author(s): 円谷 健, 大栗 博毅, 上原 久俊, 南雲 陽子, 丸山 潤美, 新藤 由美, 佐々木 信也, 井上 将行, 平間 正博, 藤井 郁
  • Organizer: 第3回次世代を担う有機化学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2005-05-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Snthesis based Apioach toward Direct Sandwich Imunoassav for elguatnxins (2005)
  • Author(s): T. Tsumuraya, H. Oguii, H. Uehara, Y. Nagumo, M. Mai uyama, Y. Shindo, S. Sasaki, M. Inoue, M. Hirama, I. Fujii
  • Organizer: 3rd ymposium of Organic Chemistry, the Next Generation
  • Place of Presentation: Tokyo
  • Year and Date: 2005-05-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] SYNTHESIS-BASED APPROACH TOWARD DIRECT SANDWICH IMM-UNOASSAY FOR CIGUATOXINS (2005)
  • Author(s): Tsumuraya, T, Oguri, H, Satoh, T, Oshiro, N, Sasaki, T, Inoue, M, Fujii, I, Hirama, M
  • Organizer: Marine and Fresh water Toxins Analysis First Joint Symposium and AOAC Task Force Meeting
  • Place of Presentation: Baiona,Spain
  • Year and Date: 2005-04-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] SYNTHESIS-BASED APPROACH TOWARD DIRECT SANDWICH IMMUNOASSAY FOR CIGUATOXINS (2005)
  • Author(s): T. Tsumuraya, H. Oguri, T. Satoh, N. Oshiro, T. Sasaki, M. Inoue, I. Fujii, M. Hirama
  • Organizer: Marine and Fieshwater Toxins Analysis First Joint Symposium and AOAC Task Force Meeting
  • Place of Presentation: Baiona, Spain
  • Year and Date: 2005-04-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.b.s.osakafu-u.ac.jp/~fujii/