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2006 Fiscal Year Final Research Report Summary

Formation of isolated DNA bases pairs and their water clusters

Research Project

Project/Area Number 17550017
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical chemistry
Research InstitutionGraduate School, Yokohama City University

Principal Investigator

SAIGUSA Hiroyuki  Yokohama City University, Graduate School of Arts and Sciences, Professor, 国際総合科学研究科, 教授 (90162180)

Co-Investigator(Kenkyū-buntansha) TACHIKAWA Masanori  Yokonarna city Vnversity, Graduate School of Artsand Scoences, Associate Professor, 国際総合科学研究科, 教授 (00267410)
Project Period (FY) 2005 – 2006
KeywordsLaser Desoption / DNA bases / Hydrated clusters / Nucleosides / Tautomerization
Research Abstract

We have developed a novel laser desorption method in combination with the supersonic-jet technique for producing cold isolated clusters of the DNA bases and their hydrated clusters. This method is found to be especially effective in producing hydrated clusters of the nonvolatile molecules and thus useful in the microscopic understanding of hydration structures of DNA. The results show that excited-state dynamics of the base pairs are strongly dependent on the tautomers of the respective bases. For example, the purine base guanine reveals a tautomers of the canonical form whose excited-state lifetime is on the order of subpicosecond while other tautomers of non-canonical forms have nanosecond lifetimes. Furthermore, excited-state dynamics of adenine are altered significantly upon hydration. This is explained by the ultrafast relaxation of the initially excited state to a low-lying excited state and subsequent dissociation. This is found to be consistent with the result for 2-aminopurine in which no ultrafast dynamics occurs and thus is known to be strongly fluorescent.
We have also succeeded in producing hydrated clusters of the DNA nucleoside guanosines with high efficiency by employing this desorption technique. This is the first report of the observation of cold hydrated clusters of the nucleosides under isolated conditions. The electronic spectra of guanosines are found to be significantly red-shifted upon hydration, suggesting that the excited state-dynamics are affected by the solvation.
This laser desorption technique is expected to be effective for vaporizing thermally fragile biomolecules

  • Research Products

    (5 results)

All 2007 2006

All Journal Article (5 results)

  • [Journal Article] Tautomerization behavior in guanine and methylated guanines, and its relation to the base pairing and hydration2007

    • Author(s)
      K.Miyazaki, N.Mizuno, T.Katayama, K.Takahashi, M.Tachikawa, H.Saigusa
    • Journal Title

      Physical Chemistry and Chemical Physics (submitted)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Excited-State Dynamics of Nucleic Acid Bases and Their Clusters2006

    • Author(s)
      H.Saigusa
    • Journal Title

      Journal of Photochemistry and Photobiology C : Photochemistry Reviews 7

      Pages: 197-210

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A matrix-free laser desorption method for production of nucleobase clusters and their hydrates2006

    • Author(s)
      H.Saigusa, A.Tomioka, T.Katayama, E.Iwase
    • Journal Title

      Chemical Physics Letters 418・1

      Pages: 119-125

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Excited-state dynamics of nucleic acid base and their clusters2006

    • Author(s)
      H.Saigusa
    • Journal Title

      Journal of Photochemistry and Photobiology C : Photochemistry Reviews 7

      Pages: 197-210

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A matrix-free laser desorption method for production of nucleobase clusters and their hydrates2006

    • Author(s)
      H.Saigusa, A.Tomioka, T.Katayama, E.Iwase
    • Journal Title

      Chemical Physics Letters 418(1)

      Pages: 119-125

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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