2007 Fiscal Year Final Research Report Summary
Thermodynamic Analysis of Separation Behavior in Reversed-Phase Liquid Chromatography
| Project/Area Number |
17550075
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Toyama |
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Principal Investigator |
MIYABE Kanji University of Toyama, Graduate School of Science and Engineering for Research, Professor (10281015)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Reversed-phase liquid chromatography / Retention equilibrium / Surface diffusion / Enthalpy - entropy compensation / Linear free energy relationship / Thermodynamic properties / Mass transfer |
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| Research Abstract |
Surface diffusion coefficients ware measured in reversed-phase liquid chromatography systems using surface modified silica gels and methanol/water mixtures by applying the pulse response-moment analysis method and the peak parking method. The values of surface diffusion coefficient ware analyzed from the viewpoints of mass transfer kinetics, thermodynamics, and extra-thermodynamics. A model was developed by analyzing the experimental data of surface diffusion coefficients thus measured. A method based on the model was proposed for estimating the value of surface diffusion coefficient Surface diffusion coefficient can be estimated from the information about the retention equilibrium with a relative error less than 30%.
Various types of separation media having different structural characteristics have been used. The moment analysis theory can provide some items of quantitative information about the retention equilibrium and the mass transfer kinetics in the chromatographic systems using t
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he different packing materials. In this study, a series of new moment equations were systematically derived for the chromatographic systems using the various types of separation media by considering their shapes (spherical particle, cylindrical fiber, and flat plate) and porous structures (full-porous, partially-porous (pellicular), and non-porous). It was tried to demonstrate the usefulness of the new moment equations for evaluating the chromatographic performance of the various packing materials having the different structural characteristics as the stationary phase for the fast HPLC with a high efficiency We can use the new moment equations for the quantitative prediction of the chromatographic behavior of the various packing materials under different conditions of chromatography and evaluate their performance from the various points of view. The new moment equations are effective not only for the detailed analyses of chromatographic behavior but also for the preliminary evaluation of new types of separation media for HPLC. Less
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