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2007 Fiscal Year Final Research Report Summary

Thermodynamic Analysis of Separation Behavior in Reversed-Phase Liquid Chromatography

Research Project

Project/Area Number 17550075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionUniversity of Toyama

Principal Investigator

MIYABE Kanji  University of Toyama, Graduate School of Science and Engineering for Research, Professor (10281015)

Project Period (FY) 2005 – 2007
KeywordsReversed-phase liquid chromatography / Retention equilibrium / Surface diffusion / Enthalpy - entropy compensation / Linear free energy relationship / Thermodynamic properties / Mass transfer
Research Abstract

Surface diffusion coefficients ware measured in reversed-phase liquid chromatography systems using surface modified silica gels and methanol/water mixtures by applying the pulse response-moment analysis method and the peak parking method. The values of surface diffusion coefficient ware analyzed from the viewpoints of mass transfer kinetics, thermodynamics, and extra-thermodynamics. A model was developed by analyzing the experimental data of surface diffusion coefficients thus measured. A method based on the model was proposed for estimating the value of surface diffusion coefficient Surface diffusion coefficient can be estimated from the information about the retention equilibrium with a relative error less than 30%.
Various types of separation media having different structural characteristics have been used. The moment analysis theory can provide some items of quantitative information about the retention equilibrium and the mass transfer kinetics in the chromatographic systems using t … More he different packing materials. In this study, a series of new moment equations were systematically derived for the chromatographic systems using the various types of separation media by considering their shapes (spherical particle, cylindrical fiber, and flat plate) and porous structures (full-porous, partially-porous (pellicular), and non-porous). It was tried to demonstrate the usefulness of the new moment equations for evaluating the chromatographic performance of the various packing materials having the different structural characteristics as the stationary phase for the fast HPLC with a high efficiency We can use the new moment equations for the quantitative prediction of the chromatographic behavior of the various packing materials under different conditions of chromatography and evaluate their performance from the various points of view. The new moment equations are effective not only for the detailed analyses of chromatographic behavior but also for the preliminary evaluation of new types of separation media for HPLC. Less

  • Research Products

    (11 results)

All 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (5 results)

  • [Journal Article] Evaluation of Chromatographic Performance of Various Packing Materials Having Different Stuctural Characteristics as Stationary Phase for Fast High Performance Liquid Chromatogr aphy by New Moment Equations.2008

    • Author(s)
      Miyabe, K.
    • Journal Title

      J. Chromatogr. A 1183

      Pages: 49-64

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Evaluation of Chromatographic Performance of Various Packing Materials Having Different Structural Characteristics as Stationary Phase for Fast High Performance Liquid Chromatography by New Moment Equations2008

    • Author(s)
      Miyabe, K
    • Journal Title

      J. Chromatogr. A 1183

      Pages: 49-64

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Surface Diffusion in Reversed-Phase Liquid Chromatography Using Silica Gel Stationary Phaces of Different C_1 and C_<18> Ligand Densities.2007

    • Author(s)
      Miyabe, K.
    • Journal Title

      J. Chromatogr. A 1167

      Pages: 161-170

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] New Moment Equations for Chromatography Using Various Stationary Phases of Different Structural Characteristics.2007

    • Author(s)
      Miyabe, K.
    • Journal Title

      Anal. Chem. 79

      Pages: 7457-7472

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Surface Diffusion in Reversed-Phase Liquid Chromatography Using Silica Gel Stationary Phases of Different C_1 and C_18 Ligand Densities2007

    • Author(s)
      Miyabe, K
    • Journal Title

      J. Chromatogr. A 1167

      Pages: 161-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] New Moment Equations for Chromatography Using Various Stationary Phases of Different Structural Characteristics2007

    • Author(s)
      Miyabe, K
    • Journal Title

      Anal. Chem 79

      Pages: 7457-7472

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] New Moment Equations for Chromatography Using Various Separation media having Different Structural Characteristics of Shapes and Porosities2007

    • Author(s)
      Miyabe, K
    • Organizer
      18th Annual meeting of The Society for Chromatographic Sciences
    • Place of Presentation
      Hakodate
    • Year and Date
      2007-11-09
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Evaluation of Chromatographic Performance of Various Packing Materials Having Different Structural Characteristics as Stationary Phase for Fast High Performance Liquid Chromatography by New Moment Equations2007

    • Author(s)
      Miyabe, K
    • Organizer
      18th Annual meeting of The Society for Chromatographic Sciences
    • Place of Presentation
      Hakodate
    • Year and Date
      2007-11-09
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 様々な構造的特徴を有する固定相を用いるクロマトグラフィー系に対する新規モーメント解析式の開発2007

    • Author(s)
      宮部寛志
    • Organizer
      日本分析化学会第56年会
    • Place of Presentation
      徳島大学
    • Year and Date
      2007-09-19
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Development of New Moment Equations for Chromatography Using Various Stationary Phases of Different Structural Characteristics2007

    • Author(s)
      Miyabe, K
    • Organizer
      56th Annual meeting of Japan Society for Analytical Chemistry
    • Place of Presentation
      Tokushima
    • Year and Date
      2007-09-19
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Thermodynamic Study on Surface Diffusion in Reversed-Phase Liquid Chromatography2007

    • Author(s)
      Miyabe, K
    • Organizer
      56th Annual meeting of Japan Society for Analytical Chemistry
    • Place of Presentation
      Tokushima
    • Year and Date
      2007-09-19
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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