2006 Fiscal Year Final Research Report Summary
A genetic analysis on a novel regulatory mechanism to induce cell death in response to perturbation of dynamic instability of microtubules
| Project/Area Number |
17570006
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Genetics/Genome dynamics
|
|---|
| Research Institution | Kyoto Institute of Technology |
|---|
Principal Investigator |
INOUE Yoshihiro Kyoto Institute of Technology, Drosophila Genetic Resource Center, Lecturer, ショウジョウバエ遺伝資源センター, 講師 (90201938)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Masamitsu Kyoto Institute of Technology, Graduate School of Science and Technology, Professor, 工芸科学研究科, 教授 (00182460)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Keywords | microtubules / Drosophila / apoptosis / Orbit / dynamic instability |
|---|
| Research Abstract |
Microtubules are indeed involved in many cellular phenomena. It has been shown that dynamic instability of microtubules is especially important for regulation of some cellular events. The Orbit/CLASP, a conserved microtubule-associated protein enriched at plus-ends of microtubules plays an essential role in regulation of the dynamic instability. Loss-of-function mutations of the orbit in Drosophila increase instability of microtubules. Conversely, over-expression of the gene in imaginal discs using GAL4-UAS system causes excessive stabilization of microtubules and consequently a formation of microtubule bundles that are never observed in normal cells at interphase. The over-expression of the orbit in eye disc cells after morphogenic furrows accumulates metaphase cells, suggesting it activates the microtubule assembly checkpoint in mitosis. We found that the over-expression eventually induces apoptosis dependent on Caspase 3 at post-mitotic stage in the eye discs. Knockdown of the gene also causes induction of the Caspase 3 in wing discs. This type of apoptosis cannot be observed in non-dividing cells such as larval salivary gland cells with over-expression of the orbit. The induction of apoptosis is required for a proper function of mitotic checkpoint genes and p53. Our data should suggest that cells with abnormal microtubules deviated from proper range of the dynamic instability could be recognized during mitosis and could be eliminated by the apoptosis from proliferating tissues.
|
Research Products
(24 results)
-
[Journal Article] Transcriptional regulation of the Drosophila rfc1 gene by the DRE-DREF pathway.2007
Author(s)
Tsuchiya, A., Inoue, Y.H., Ida, H., Kawase, Y., Okudaira, K., Ohno, K., Yoshida, H., Yamaguchi, M.
-
Journal Title
FEBS J. 274
Pages: 1818-1832
Description
「研究成果報告書概要(和文)」より
-
[Journal Article] Complex interference in the eye developmental pathway by Drosophila NF-YA.2007
Author(s)
Yoshioka, Y., Suyari, O., Yamada M., Ohno, K., Hayashi, Y., Yamaguchi, M.
-
Journal Title
Description
「研究成果報告書概要(和文)」より
-
[Journal Article] Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system.2007
Author(s)
Kim, Y.S, Shin, M.J., Yang, D.J., Yamaguchi, M., Park, S.Y., Yoo, M.A.
-
Journal Title
Genes to Cells. (in press)
Description
「研究成果報告書概要(和文)」より
-
[Journal Article] Prevalence of full-size P and KP elements in North America populations of Drosophila melanogaster.2007
Author(s)
Itoh, M., Takeuchi, N., Yamaguchi, M., Yamamoto, M., Boussy, I., A.
-
Journal Title
Description
「研究成果報告書概要(和文)」より
-
[Journal Article] Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a.2007
Author(s)
Nishida, M., Kato, M., Kato, Y., Sasai, N., Ueda, J., Tachibana, M., Shinkai, Y., Yamaguchi, M.
-
Journal Title
Genes to Cells. (in press)
Description
「研究成果報告書概要(和文)」より
-
[Journal Article] Transcriptional regulation of the Drosophila rfcl gene by the DRE-DREF pathway2007
Author(s)
Tsuchiya, A., Inoue, Y.H., Ida, H., Kawase, Y., Okudaira, K., Ohno, K., Yoshida, H, Yamaguchi, M.
-
Journal Title
FEBS J. 274
Pages: 1818-1832
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Complex interference in the eye developmental pathway by Drosophila NF-YA.2007
Author(s)
Yoshioka, Y, Suyari, O., Yamada, M., Ohno, K., Hayashi, Y., Yamaguchi, M.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Drosophila Damaged DNA Binding Protein 1 contributes to genome stability in somatic cells.2006
Author(s)
Shimanouchi, K., Takata, K., Yamaguchi, M., Murakami, S., Ishikawa, G., Takeuchi, R., Kanai, Y., Ruike, T., Nakamura, R., Abe, Y., Sakaguchi, K.
-
Journal Title
J. Biochem. 139
Pages: 51-58
Description
「研究成果報告書概要(和文)」より
-
-
-
-
-
-
[Journal Article] Drosophila Damaged DNA Binding Protein 1 contributes to genome stability in somatic cells.2006
Author(s)
Shimanouchi, K., Takata, K., Yamaguchi, M., Murakami, S., Ishikawa, G, Takeuchi, R., Kanai, Y, Ruike, T, Nakamura, R Abe, Y.Sakaguchi, K.
-
Journal Title
J.Biochem. 139
Pages: 51-58
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
[Journal Article] Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system.
Author(s)
Kim, Y.S, Shin, M.J., Yang, D.J., Yamaguchi, M., Park, S.Y., Yoo, M.A.
-
Journal Title
Genes to Cells (in press)
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Prevalence of full-size P and KP elements in North America populations of Drosophila melanogaster.
Author(s)
Itoh, M., Takeuchi, N., Yamaguchi, M., Yamamoto, M., Boussy, I., A.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Identification of ZNF200 as a novel binding partner of histone H3 methyltransferase G9a.
Author(s)
Nishida, M., Kato, M., Kato, Y., Sasai, N., Ueda, J., Tachibana, M., Shinkai, Y., Yamaguchi, M.
-
Journal Title
Genes to Cells (in press)
Description
「研究成果報告書概要(欧文)」より
-
