• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

The sex-determining gene of amphibians having the XY-type sex-determining system differs from that of ones having the ZW-type sex-determining system

Research Project

Project/Area Number 17570053
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionWaseda University

Principal Investigator

NAKAMURA Masahisa  Waseda University, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Assistant, 教育・総合科学学術院, 教授 (40130025)

Co-Investigator(Kenkyū-buntansha) KOBAYASI Masaki  Waseda University, Department of Biology, Faculty of Education and Integrated Arts and Sciences, Reserch Associate, 教育・総合科学学術院, 助手 (80373041)
MATSUI Kumi  Azabu University, Faculty of Veterinary, Department of Physiology, Assistant Professor., 獣医学部, 講師 (70367019)
Project Period (FY) 2005 – 2006
KeywordsSex-determining gene / Karyotype / Sex differentiation / FISH / RT-PCR / cDNA / Amphibian / Rana rugosa
Research Abstract

The aim of this research was to isolate the sex-determining gene in the frog Rana rugosa having two sex-determining systems (the XY and ZW types). We thought that the Dmrt1 gene was the best candidate for the sex-determining gene in R. rugosa having the ZW-type sex-determining system, since this gene was considered to be the sex-determining gene in chickens. However, FISH analysis revealed that Dmrt1 was an autosomal gene. The sex of the ZW type R. rugosa can be reversed from male to female by estrogens, whereas that of the XY type R. rugosa can be changed from female to male by androgens. However, the mechanism of the sex-reversal is largely unknown. To clarify the mechanism of the sex-reversal, we cloned and characterized 3 genes encoding sex steroid-synthesizing enzymes, i.e., CYP11A1, CYP17 and 3βHSD from the frog R. rugosa. RT-PCR analysis showed that the CYP17 expression was higher in male gonads during sex determination in R. rugosa, whereas CYP11A1 and 313-HSD showed no sexually dimorphic expression. When the sex was changed from female to male by injection of testosterone, the level of CYP17 transcripts increased in the sex-reversed gonad, whereas there was no change in the levels of CYP11A1 and 3β-HSD transcripts. In addition, in situ hybridization analysis revealed that CYP17 was expressed in somatic cells of the indifferent male gonad and in those of the testis. Positive signals of CYP17 were also produced in somatic cells of a female to male sex-reversed gonad (testis), but not in the ovary. Taken together, the results suggest that CYP17 is closely involved in testicular differentiation of the gonad in R. rugosa. CYP17 is the key-gene for female-to-male sex-reversal in R. rugosa.

  • Research Products

    (14 results)

All 2007 2006

All Journal Article (14 results)

  • [Journal Article] Expression of DMRT genes in the gonads of Rana rugosa during sex determination.2007

    • Author(s)
      Y.Matsushita, Y.Oshima, M.Nakamura
    • Journal Title

      Zoological Science 24

      Pages: 95-99

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of Lhx9 isoforms in the developing gonads of Rana rugosa.2007

    • Author(s)
      Y.Oshima, K.Noguchi, M.Nakamura
    • Journal Title

      Zoological Science (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Elevated expression of P450c17 (CYP17) during testicular formation in the frog.2007

    • Author(s)
      R.Iwade, K.Maruo, G.Okada, M.Nakamura
    • Journal Title

      Gen. Comp. Endocrinol. (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Chromosomal localization of eight sexual differentiation genes in the population of Japanese wrinkled frog (Rana rugosa) ZZ/ZW sex chromosomal system.2007

    • Author(s)
      T.Murakami, C.Nishida-Umehara, K.Noguchi, Y.Matsuda, M.Nakamura
    • Journal Title

      Chromosome Research (Submitted)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stage-dependent expression of Zfp64 and Zfp112 in growing oocytes of the frog Rana rugosa.2007

    • Author(s)
      G.Okada, K.Maruo, S.Funada, M.Nakamura
    • Journal Title

      Gen. Comp. Endocrinol. (Submitted)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of DMRT genes in the gonads of Rana rugosa during sex determination.2007

    • Author(s)
      Y.Matsushita, Y.Oshima, M.Nakamura.
    • Journal Title

      Zool. Sci. 24

      Pages: 95-99

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] isoforms in the developing gonads of Rana rugosa.2007

    • Author(s)
      Y.Oshima, K.Noguchi, M.Nakamura.Expression of Lhx9
    • Journal Title

      Zool. Sci. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] expression of P450c17 (CYP17) during testicular formation in the frog.2007

    • Author(s)
      R.Iwade, K.Maruo, G.Okada, M.Nakamura.Elevated
    • Journal Title

      Gen. Comp. Endocrinol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] M. Nakamura. Chromosomal localization of eight sexual differentiation genes in the population of Japanese wrinkled frog (Rana rugosa) ZZ/ZW sex chromosomal system.2007

    • Author(s)
      T.Murakami, C.Nishida-Umehara, K.Noguchi, Y.Matsuda,
    • Journal Title

      Chromosome Res.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Stage-dependent expression of Zfp64 and Zfp112 in growing oocytes of the frog Rana rugosa.2007

    • Author(s)
      G.Okada, K.Maruo, S.Funada, M.Nakamura.
    • Journal Title

      Gen. Comp. Endocrinol.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (P450arom) gene.2006

    • Author(s)
      Y.Oshima, T.Kato, D.Wang, T.Murakami, Y.Matsuda, Y.Nagahama, M.Nakamura, Y.Nagahama, M.Nakamura
    • Journal Title

      Zoological Science 23

      Pages: 79-85

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pituitaryadenylate cyclase-activating polypeptide-induced differentiation of embryonic neural stem cells into astrocytes is mediated via the beta isoform of protein kinase C.2006

    • Author(s)
      J.Watanabe, M.Ohba, F.Ohno, S.Kikuyama, M.Nakamura et al.
    • Journal Title

      Journal of Neuroscience Research 84

      Pages: 1645-1655

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Promoter activity and chromosomal location of the Rana rugosa P450 aromatase (P450arom) gene.2006

    • Author(s)
      Y.Oshima, T.Kato, D.Wang, T.Murakami, Y.Matsuda, Y.Nagahama, M.Nakamura.
    • Journal Title

      Zool. Sci. 23

      Pages: 79-85

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Pituitary adenylate cyclase-activating polypeptide-induced differentiation of embryonic neural stem cells into astrocytes is mediated via the beta isoform of protein kinase C.2006

    • Author(s)
      J.Watanabe, M.Ohba, F.Ohno, S.Kikuyama, M.Nakamura, K.Nakaya, A.Arimura, S.Shioda, S.Nakajo.
    • Journal Title

      J. Neurosci. Res. 84

      Pages: 1645-1655

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2008-05-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi