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2007 Fiscal Year Final Research Report Summary

Analyses of visual pigments and G proteins coupled with them in light-sensitive chromatophores of teleost fishes

Research Project

Project/Area Number 17570068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionToho University

Principal Investigator

OSHIMA Noriko  Toho University, Faculty of Science, Professor (70057735)

Co-Investigator(Kenkyū-buntansha) IWAMURO Shouichi  Toho University, Faculty of Science, Associate Professor (70221794)
Project Period (FY) 2005 – 2007
Keywordslight-sensitive chromatophores / visual pigment / Nile tilapia erythrophores / neon tetra iridophores / cyclic AMP / G protein / Gi / opsin
Research Abstract

1. In Nile tilapia erythrophores, pigment aggregation is caused by light at 400 and 600 nm and dispersion occurred in response to light at 500 nm. We found the expression of mRNAs of cone opsins, red, green and blue, in the chromatophores. Further neon tetra iridophores present in the lateral stripe skin are also light-sensitive, and light near 500 nm is most effective. RT-PCR showed the expression of mRNAs encoding rhodopsin and two kinds of cone opsins (Pi-green 1 and Pi green 2) in neon tetra skin where the iridophores exist. These mRNAs are also expressed in the lateral eyes. The cone opsin genes, Pi-green 1 and Pi-green 2, show high similarity with the g101 and g102 genes of unique green cone opsins (belonging to the MWS/LWS group) of the blind Mexican cavefish The identity of amino acid sequences between Pi-green 1 and Pi-green 2 is considered to be more than 90% The red opsin gene was not expressed in neon tetra skin.
2. Using anti-opsin (MWS/LWS) and anti-rhodopsin antibodies, western blotting analysis also demonstrated the presence of opsins in the skin where iridophores exist, and the localization of the opsins in iridophores was confirmed by immunohistochemical analysis.
3. The involvement of cyclic AMP in the phototransduction was indicated using various inhibitors and activators of enzymes participating in the transduction. That is, light stimulation was accompanied by the decrease in cAMP level in the light-sensitive chromatophores. The treatment of Nile tilapia etythrophores and neon tetra iridophores with bacteria toxins also suggested the important role of Gi in the phototransduction.
4. We also confirmed the expression of Gi mRNA and Gi protein in neon tetra iridophores by RT-PCR and western blotting respectively. Although partial expression of Gt mRNA was confirmed, Gt protein was not detected in the iridophores of neon tetra.

  • Research Products

    (20 results)

All 2007 2006 2005

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (10 results) Book (1 results)

  • [Journal Article] Expression of the estrogen receptor gene in the anal fin of Japanese medaka,Oryzias latipes2007

    • Author(s)
      H. Hayashi, A. Nishimoto, N. Oshima, S. Iwamuro
    • Journal Title

      Journal of Toxicological Science 32

      Pages: 91-96

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression of the estrogen receptor gene in the anal fin of Japanese medaka, Oryzias latipes2007

    • Author(s)
      H., Hayashi, A., Nishimoto, N., Oshima, S., Iwamuro
    • Journal Title

      Journal of Toxicological Science 32

      Pages: 91-96

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Light-sensitive motile iridophores and visual pigment in the neon tetra,Paracheirodon innesi2006

    • Author(s)
      A. Kasai, N. Oshima
    • Journal Title

      Zoologocal Science 23

      Pages: 815-819

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Color and distribution of pigment cells in the skin of the zebrafish2006

    • Author(s)
      K. Miyaji, M. Kimura, N. Oshima
    • Journal Title

      Journal of SJWS 6

      Pages: 22-28

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Light-sensitive motile iridophores and visual pigments in the neon tetra, Paracheirodon innesi2006

    • Author(s)
      A., Kasai, N., Oshima
    • Journal Title

      Zoological Science 23

      Pages: 815-819

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Color and distribution of pigment cells in the skin of the zebrafish2006

    • Author(s)
      K., Miyaji, M., Kimura, N., Oshima
    • Journal Title

      Journal of SJWS 6

      Pages: 22-28

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The signaling pathway in photoresponses that may be mediated by visual pigments in erythrophores of Nile tilapia2005

    • Author(s)
      E. Ban, A. Kasai, M. Sato, A. Yokozeki, O. Hisatomi, N. Oshima
    • Journal Title

      Pigment Cell Research 18

      Pages: 360-369

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The signaling pathway in photoresponses that may be mediated by visual pigments in erythrophores of Nile tilapia2005

    • Author(s)
      E., Ban, A., Kasai, M., Sato, A., Yokozeki, O., Hisatomi, N., Oshima
    • Journal Title

      Pigment Cell Research 18

      Pages: 360-369

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Chapter V : Light reflection in motile iridophores of fish2005

    • Author(s)
      N., Oshima
    • Journal Title

      In : "Structural Colors in Biological Systems-Principles and Applications" (S. Kinoshita and S. Yoshioka eds.), Osaka University Press

      Pages: 211-229

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ネオンテトラ運動性虹色素胞の光応答反応と視物質遺伝子2007

    • Author(s)
      河西 亜希子・大島 範子
    • Organizer
      日本動物学会第78回大会
    • Place of Presentation
      弘前
    • Year and Date
      20070900
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Photoresponses and visual pigments in motile iridohpores of the neon tetra2007

    • Author(s)
      A., Kasai, N., Oshima
    • Organizer
      78th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Hirosaki
    • Year and Date
      20070900
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ネオンテトラ運動性虹色素胞における光の直接受容とそのシグナル伝達について2006

    • Author(s)
      河西 亜希子・大島 範子
    • Organizer
      第20回日本色素細胞学会年次学術大会
    • Place of Presentation
      松本
    • Year and Date
      20061100
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Study on the direct receptiOn of light and the phototransduction in motile iridohpores of the neon tetra2006

    • Author(s)
      A., Kasai, N., Oshima
    • Organizer
      20th Annual Meeting of the Japanese Society for Pigment Cell Research
    • Place of Presentation
      Matsumoto
    • Year and Date
      20061100
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ネオンテトラ運動性虹色素胞における光情報伝達系に関する研究2006

    • Author(s)
      河西 亜希子・大島 範子
    • Organizer
      日本動物学会第77回大会
    • Place of Presentation
      島根
    • Year and Date
      20060900
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Study on the phototransduction in motile iridohpores of the neon tetra2006

    • Author(s)
      A., Kasai, N., Oshima
    • Organizer
      77th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Shimane
    • Year and Date
      20060900
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ネオンテトラ真皮運動性虹色素胞の光受容機構に関する研究2005

    • Author(s)
      河西 亜希子・大島 範子
    • Organizer
      日本動物学会第76回大会
    • Place of Presentation
      筑波
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Study on the mechanism of light-reception in motile iridohpores in dermis of the neon tetra2005

    • Author(s)
      A., Kasai, N., Oshima
    • Organizer
      76th Annual Meeting of the Zoological Society of Japan
    • Place of Presentation
      Tsukuba
    • Year and Date
      20051000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Study on expression of visual pigment genes in light-sensitive iridophores in the dermic of neon tetra2005

    • Author(s)
      A. Kasai, N. Oshima
    • Organizer
      19th International Pigment Cell Conference
    • Place of Presentation
      Reston,Virginia,USA
    • Year and Date
      20050900
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Study on expression of visual pigment genes in light-sensitive iridophores in the dermis of neon tetra2005

    • Author(s)
      A., Kasai, N., Oshima
    • Organizer
      19th International Pigment Cell Conference, September
    • Place of Presentation
      Reston, Virginia, USA
    • Year and Date
      20050900
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Light reflection in motie iridophores of fish.In:"Structural Colors in Biological Systems-Principles and Applications"(S.Kinoshita and S.Yoshioka eds.)2005

    • Author(s)
      N. Oshima
    • Total Pages
      19(全体は351)
    • Publisher
      Osaka University Press
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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