2007 Fiscal Year Final Research Report Summary
Study on the reproductive isolation and speciation of the Fejervarya limnocharis complex from Asia
| Project/Area Number |
17570082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Hiroshima University |
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Principal Investigator |
SUMIDA Masayuki Hiroshima University, Graduate School of Science, Professor (10163057)
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| Co-Investigator(Kenkyū-buntansha) |
KURABAYASHI Atsushi Hiroshima University, Graduate School of Science, Assistant Professor (00327701)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Fejervarya limnocharis complex / Asia / Reproductive isolation / Mitochondrial DNA / Molecular phylogeny / Speciation / Genetic divergence / Allozyme |
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| Research Abstract |
The rice frog (Fejervarya limnocharis) is one of the most widely distributed species among Asian frogs. While there is little morphological difference within the species, there are significant differences in mating calls and genetic traits between and within populations of this species. Therefore Fejervarya limnocharis (henceforth referred to as the F. limnocharis complex) is considered to be a composite of many different species. The purpose of this study is to determine (1) which species is exactly referred to as the scientific name of Fejervarya limnocharis, (2) how much genetic divergence occurs in this complex from the entire Asia, and what geographical distribution patterns it has, (3) what correlation the genetic divergence of this complex has with the occurrence and mechanism of reproductive isolation, and (4) whether it is valid to separate this species complex from the genus Rana and include it in the genus Fejervarya. In this study, first, samples of the E limnocharis comple
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x were collected from a wide range of distribution areas in Asia. Using them, extensive hybridization experiments were performed. Based on the results, the occurrence and mechanism of reproductive isolation as well as its levels were identified. Consequently, the species diversity of the complex was directly demonstrated based on the biological species concept. The diversity of the species could be uniquely demonstrated in terms of reproductive isolation mechanism only in the Institute for Amphibian Biology, because our Institute has a complete set of breeding equipment and hybridization systems. Also in this study, DNA and allozyme analyses and morphometric measurements were performed to estimate the degree of genetic divergence within the complex. Comparing these. estimations with the results of the above hybridization experiments, the diversity of the complex was demonstrated from multiple viewpoints and a more objective and reliable system was established for classification of the complex that had not been clearly defined. The present results can greatly contribute to the studies on diversity and classification in the species complex of many animals. Less
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[Journal Article] Genetic divergence and evolutionary relationships in six species of genera Hoplobatrachus and Euphlyctis(Amphibia: Anura)from Bangladesh and other Asian countries revealed by mitochondrial gene sequences2008
Author(s)
Alam, M.S., Igawa, T., Khan, M.M.R., Islam, M.M., Kuramoto, M., Matsui, M., Kurabayashi, A. and Sumida, M.
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Journal Title
Molecular Phylognetics and Evolution 47(In press)
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Genetic divergences and phylogenetic relationships of the Fejervatya Limnocharis complex in Thailand and neighboring countries revealed by mitochondrial and nuclear genes2008
Author(s)
Kotaki, M., Kurabayashi, A., Matsui, M., Khonsue, W., Djong, T.H., Tandon, M., Sumida, M
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Journal Title
Zoological Science 25
Pages: 381-390
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic divergence and evolutionary relationships in six species of genera Hoplobatrachus and Euphlyctis (Amphibia : Anura) from Bangladesh and other Asian countries revealed by mitochondrial gene sequences2008
Author(s)
Alam, M.S., Igawa, T., Khan, M.M.R., Islam, M.M., Kuramoto, M., Matsui, M., Kurabayashi, A., Sumida, M
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Journal Title
Molecular Phylognetics and Evolution 47 (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic relationships and reproductive isolation mechanisms among the Fejervarya limnocharis complex from Indonesia(Java)and other Asian countries2007
Author(s)
Djong, H.T., M.M. Islam, M. Nishioka, M. Matsui, H. Ota, M. Kuramoto, M.M.R. Khan, M.S. Alam, A. De Silva, W. Khonsue and M. Sumida
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Journal Title
Zoological Science 24(3)
Pages: 360-375
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Evolutionary relationships and reproductive isolating mechanisms in the rice frog Fejervarya limnocharis complex from Sri Lanka, Thailand, Taiwan and Japan inferred from mtDNA gene sequence analysis, allozyme analysis and crossing experiments2007
Author(s)
Sumida, M., M. Kotaki, M.M. Islam, H.T. Djong, T. Igawa, Y. Kondo, M. Matsui, A. De Silva, W. Khonsue and M. Nishioka
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Journal Title
Zoological Science 24(5)
Pages: 547-562
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Genetic relationships and reproductive isolation mechanisms among the Fejervarya limnocharis complex from Indonesia (Java) and other Asian countries2007
Author(s)
Djong, T.H., Islam, M.M., Nishioka, M., Matsui, M., Ota, H., Kuramoto, M., Khan, M.M.R., Alam, M.S., Anslem, D.S., Khonsue, W., Sumida, M
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Journal Title
Zoological Science 24
Pages: 360-375
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evolutionary relationships and Reproductive isolating mechanisms in the rice frog Fejervarya limnocharis complex From Sri Lanka, Thailand, Taiwan and Japan inferred from mtDNA gene sequence analysis, allozyme analysis and crossing experiments2007
Author(s)
Sumida, M., Kotaki, M., Islam, M.M., Djong, T.H., Igawa, T., Kondo, Y., Matsui, M., Anslem, D.S., Khonsue, W., Nishioka, M
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Journal Title
Zoological Science 24
Pages: 547-562
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Morphological divergence, reproductive isolating mechanism and molecular phylogenetic relationship among Indonesia, Malaysia and Japan populations of the Fejervarya limnocharis complex (Anura, Ranidae)2007
Author(s)
Djong, T.H., Matsui, M., Kuramoto, M., Belabut, D.M., Yong, H.S., Nishioka, M., Sumida, M
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Journal Title
Zoological Science 24
Pages: 1197-1212
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Complete nucleotide sequence of the mitochondrial genome of a Malagasy poison frog Mantella madagascariensis: Evolutionary implications on mitochondrial genomes of higher anuran groups2006
Author(s)
Kurabayashi, A., Usuki, C., Mikami, N., Fujii, T., Yonekawa, H., Sumida, M. and Hasegawa M.
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Journal Title
Molecular Phylognetics and Evolution 39
Pages: 223-236
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Complete nucleotide sequence of the mitochondrial genome of a Malagasy poison frog Mantella madagascariensis : Evolutionary implications on mitochondrial genomes of higher anuran groups2006
Author(s)
Kurabayashi, A., Usuki, C., Mikami, N., Fujii, T., Yonekawa, H., Sumida, M., Hasegawa, M
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Journal Title
Molecular Phylognetics and Evolution 39
Pages: 223-236
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Complete mitochondrial genomes of three neobatrachian anurans : A case study of divergence time estimation using different data and calibration settings2007
Author(s)
Igawa, T., Kurabayashi, A., Usuki, C., Fujii, T., Sumida, M
Organizer
Asia-Africa Evolutionary Meeting
Place of Presentation
Chiba, Japan
Year and Date
2007-12-05
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic divergences and phylogenetic relationships of Fejervarya limnocharis complex and related genera from Thailand and neighboring countries revealed by nuclear and mitochondrial genes2007
Author(s)
Kotaki, M., Kurabayashi, A., Matsui, M., Khonsue, W., Djong, T.H., Manuj, Tandon, M., Sumida, M
Organizer
The 46th Anual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Intraspecific differentiation of the bell-ring frog Buergeria buergeri based on crossing experiments, morphological comparison and mtDNA analyses2007
Author(s)
Nishizawa, T., Sano, N., Kunihara, T., Fujii, T., Kurabayashi, A., Sumida, M
Organizer
The 46th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Intraspecific differentiation in an endangered species Rana ishikawae elucidated by morphological observations, crossing experiments, allozyme and mtDNA analyses2007
Author(s)
Sumida, M., Sato, N., Kurabayashi, A., Oumi, S., Ota, H., Katsuren, Sugawara, Y., Fujii, T
Organizer
The 46th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-18
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic divergences and evolutionary relationships in six species of the genera, Hoplobatrachus and Euphlyctis, from Bangladesh and other Asian countries revealed by mitochondrial gene sequences and crossing experiments2007
Author(s)
Alam, M. S., T. Igawa, Md. M. R. Khan, M. M. Islam, M. Kuramoto, M. Matsui, A. Kurabayashi, and M. Sumida
Organizer
日本爬虫両生類学会第46回大会
Place of Presentation
沖縄
Year and Date
2007-11-17
Description
「研究成果報告書概要(和文)」より
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[Presentation] Genetic divergences and speciation in Fejervarya cancrivora from Indonesia and other Asian countries inferred from morphological observations, crossing experiments and molecular techniques2007
Author(s)
Nia, K., D. M. Belabut, Yong, H. S., M. M. Islam, R. Wanichanon, Md. M. R. Khan, T. H. Djong D. T. Iskandar, M. Nishioka and M. Sumida
Organizer
日本爬虫両生類学会第46回大会
Place of Presentation
沖縄
Year and Date
2007-11-17
Description
「研究成果報告書概要(和文)」より
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[Presentation] Morphological divergences, reproductive isolating mechanisms and molecular relationships among the Indonesia, Thailand, and Bangladesh populations of the Fejervarya iskandari group2007
Author(s)
Djong, T. H., M. M. Islam, M. Kotaki, M. Matsui, M. Kuramoto, M. Nishioka, M. Sumida
Organizer
日本爬虫両生類学会第46回大会
Place of Presentation
沖縄
Year and Date
2007-11-17
Description
「研究成果報告書概要(和文)」より
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[Presentation] Antimicrobial peptides in the skin of an endangered amphibian species, Rana ishikawae2007
Author(s)
Soga, M., Okimoto, A., Fujii, T., Sugawara, Y., Ukena, K., Sato, N., Sumida, M
Organizer
The 46th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic divergences and evolutionary relationships in six species of the genera, Hoplobatrachus and Euphlyctis, from Bangladesh and other Asian countries revealed by mitochondrial gene sequences and crossing experiments2007
Author(s)
Alam, M.S., Igawa, T., Khan, M.M.R., Islam, M.M., Kuramoto, M., Matsui, M., Kurabayashi, A., Sumida, M
Organizer
The 46th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic divergences and speciation in Fejervarya cancrivora from Indonesia and other Asian countries inferred from morphological observations, crossing experiments and molecular techniques2007
Author(s)
Kumiawan, N., Belabut, D.M., Yong, H.S., Islam, M.M., Wanichanon, R., Khan, M.M.R., Djong, T.H., Iskandar, D.T., Nishioka, M., Sumida, M
Organizer
The 46th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Differentiation of the genus Fejervarya from Bangladesh inferred from morphometric data, molecular techniques and crossing experiments, with special reference to the description of a new species2007
Author(s)
Islam, M.M., Kurose, N., Alam, M.S., Khan, M.M.R., Hasan, M., Kuramoto, M., Sumida, M
Organizer
The 46th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Morphological divergences, reproductive isolating mechanisms and molecular relationships among the Indonesia, Thailand, and Bangladesh populations of the Fejervarya iskandari group2007
Author(s)
Djong, T.H., Islam, M.M., Kotaki, M., Matsui, M., Kuramoto, M., Nishioka, M., Sumida, M
Organizer
The 46th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Okinawa
Year and Date
2007-11-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Intraspecific differentiation in an endangered species Rana ishikawae2007
Author(s)
Sato, N., Oumi, S., Ota, H., Katsuren, S., Islam, M.M., Fujii, T., Kurabayashi, A., Sumida, M
Organizer
The 78th Annual Meeting of the Zoological Society of Japan
Place of Presentation
Hirosaki
Year and Date
2007-09-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of antimicrobial peptides from an endangered amphibian species, Rana ishikawae2007
Author(s)
Ukena, K., Okimoto, A., Fujii, T., Satou, N., Sumida, M
Organizer
The 78th Annual Meeting of the Zoological Society of Japan
Place of Presentation
Hirosaki
Year and Date
2007-09-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic diveregnces and phylogenetic relationships of Fejervarya limnocharis complex from Bangladesh and some other Asian countries inferred from mtDNA sequence and allozyme analyses2007
Author(s)
Islam, M.M., Kurose, N., Khan, M.M.R., Alam, M.S., Sumida, M
Organizer
Conference on Promotion of Biotechnology in Bangladesh : National and International Perspectives
Place of Presentation
Dhaka, Bangladesh
Year and Date
2007-04-07
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Molecular phylogenetic relationships among the Indian bullfrog Hoplobatrachus tigerinus and related species from Bangladesh and other Asian countries, inferred from mtDNA gene sequences2006
Author(s)
Alam, M.S., Igawa, T., Islam, M.M., Kurabayashi, A., Sumida, M
Organizer
The 45th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Higashihiroshima
Year and Date
2006-10-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Intraspecific differentiation in an endangered species Rana ishikawae2006
Author(s)
Sato, N., Oumi, S., Ota, H., Islam, M.M., Kurabayashi, A., Sumida, M
Organizer
The 45th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Higashihiroshima
Year and Date
2006-10-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Intraspecific differentiation of the bell-ring frog Buergeria buergeri based on crossing experiments, morphological observations, and mtDNA analyses2006
Author(s)
Nishizawa, T., Kunihara, T., Fujii, T., Kurabayashi, A., Sumida, M
Organizer
The 45th Annual Meeting of the Herpetologicai Society of Japan
Place of Presentation
Higashihiroshima
Year and Date
2006-10-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Evolution of the mitochondrial genomic structures in rhacophorids2006
Author(s)
Sano, N., Kurabayashi, A., Igawa, T., Fujii, T., Yonekawa, H., Sumida, M
Organizer
The 78th Annual Meeting of the Genetics Society of Japan
Place of Presentation
Tsukuba
Year and Date
2006-09-25
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Reproductive isolation mechanisms among Fejervarya limnocharis from Bangladesh and other Asian countries revealed by crossing experiments and observing meiotic chromosomes2006
Author(s)
Islam, M.M., Djong, T.H., Alam, M.S., Khan, M.M.R., Kuramoto, M. and Sumida, M.
Organizer
日本動物学会中国四国支部大会
Place of Presentation
愛媛
Year and Date
2006-05-21
Description
「研究成果報告書概要(和文)」より
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[Presentation] Reproductive isolation mechanisms among Fejervarya limnocharis from Bangladesh and other Asian countries revealed by crossing experiments and observing meiotic chromosomes2006
Author(s)
Islam, M.M., Djong, T.H., Alam, M.S., Khan, M.M.R., Kuramoto, M., Sumida, M
Organizer
The 58th Annual Meeting of the Chugoku-Shikoku Branches of the Zoological Society of Japan
Place of Presentation
Ehime
Year and Date
2006-05-21
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Genetic differentiation and speciation in the Fejervarya limnocharis complex from Bangladesh and some other Asian countries elucidated by morphological characteristics, allozyme analysis, crossing experiments and cytological observation2005
Author(s)
Islam, M.M., Alam, M.S., Djong, T.H., Sumida, M., Khan, M.M.R. and Hasanat, M.A.
Organizer
日本爬虫両棲類学会第44回大会
Place of Presentation
仙台
Year and Date
2005-11-13
Description
「研究成果報告書概要(和文)」より
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[Presentation] Genetic differentiation and speciation in the Fejervarya limnocharis complex from Bangladesh and some other Asian countries elucidated by morphological characteristics, allozyme analysis, crossing experiments and cytological observation2005
Author(s)
Islam, M.M., Alam, M.S., Djong, T.H., Sumida, M., Khan, M.M.R., Hasanat, M.A
Organizer
The 44th Annual Meeting of the Herpetological Society of Japan
Place of Presentation
Sendai
Year and Date
2005-11-13
Description
「研究成果報告書概要(欧文)」より
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[Presentation] An attempt at the artificial reproduction of Rena Ishikawae (of Amami Oshima Island), an endangered species, and the analysis of its mitochondrial DNA2005
Author(s)
Yoshikawa, N., Hayashi, Y., Kurabayashi, A., Sumida, M., Oumi, S., Fukuhara, E
Organizer
The 76th Annual Meeting of the Zoological Society of Japan
Place of Presentation
Tsukuba
Year and Date
2005-10-06
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Reproductive isolating mechanisms and genetic divergence in the rice frog Fejervarya limnocharis complex from Asia elucidated by crossing experiments and mitochondrial gene sequence analyses2005
Author(s)
Sumida, M., Islam, M.M., Kurabayashi, A., Machiyama, F., Kurose, N., Nishioka, M., Khan, M.M.R., Alam, M.S., Matsui, M., Ota, H., Kuramoto, M
Organizer
The 77th Annual Meeting of the Genetics Society of Japan
Place of Presentation
Tokyo
Year and Date
2005-09-28
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Complete nucleotide sequence of the mitochondrial DNA of Rhacophorus schlegelii (family Rhacophoridae) and structural features of rhacophorid mtDNAs2005
Author(s)
Sano, N., Kurabayashi, A., Mikami, N., Fujii, T., Yonekawa, H. and Sumida, M.
Organizer
5th World Congress of Herpetology
Place of Presentation
Stellenbosch, South Africa
Year and Date
2005-06-24
Description
「研究成果報告書概要(和文)」より
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[Presentation] Complete nucleotide sequence of the mitochondrial DNA of Rhacophorus schlegelii(family Rhacophoridae) and structural features of rhacophorid mtDNAs2005
Author(s)
Sano, N., Kurabayashi, A., Mikami, N., Fujii, T., Yonekawa, H., Sumida, M
Organizer
The 5th World Congress of Herpetology
Place of Presentation
Stellenbosch, South Africa
Year and Date
2005-06-24
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Evolution of mantellid frog mitochondrial genomes, large-scale genomic reconstruction originated within the genus Mantella2005
Author(s)
Kurabayashi, A., Usuki, C., Mikami, N., Sano, N., Fujii, T. and Sumida, M.
Organizer
5th World Congress of Herpetology
Place of Presentation
Stellenbosch, South Africa
Year and Date
2005-06-23
Description
「研究成果報告書概要(和文)」より
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[Presentation] Reproductive isolating mechanisms and genetic divergence in the rice frog Fejevarya limnocharis complex from Asia elucidated by crossing experiments and mitochondrial gene sequence analysis2005
Author(s)
Sumida, M., Machiyama, F., Kurabayashi, A., Kurose, N., Nishioka, M., Khan, M.M.R., Islam, M.M, Alam, M.S., Matsui, M., Ota, H. and Kuramoto, M.
Organizer
5th World Congress of Herpetology
Place of Presentation
Stellenbosch, South Africa
Year and Date
2005-06-23
Description
「研究成果報告書概要(和文)」より
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[Presentation] Evolution of mantellid frog mitochondrial genomes, large-scale genomic reconstruction originated within the genus Mantella2005
Author(s)
Kurabayashi, A., Usuki, C., Mikami, N., Sano, N., Fujii, T., Sumida, M
Organizer
The 5th World Congress of Herpetology
Place of Presentation
Stellenbosch. South Africa
Year and Date
2005-06-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Reproductive isolating mechanisms and genetic divergence in the rice frog Fejevarya limnocharis complex from Asia elucidated by crossing experiments and mitochondrial gene sequence analysis2005
Author(s)
Sumida, M., Machiyama, F., Kurabayashi, A., Kurose, N., Nishioka, M., Khan, M.M.R., Islam, M.M., Alam, M.S., Matsui, M., Ota, H., Kuramoto, M
Organizer
The 5th World Congress of Herpetology
Place of Presentation
Stellenbosch, South Africa
Year and Date
2005-06-23
Description
「研究成果報告書概要(欧文)」より
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