2006 Fiscal Year Final Research Report Summary
Molecular analysis of B cell receptor activation by Fab-bridge
| Project/Area Number |
17570091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
YOKOYAMA Miki Tokyo Medical and Dental University, Biochemistry, Associate Professor, 大学院医歯学総合研究科, 助教授 (70191533)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Tomoko Tokyo Medical and Dental University, Biochemistry, Technical Staff, 大学院医歯学総合研究科, 技能補佐員 (90376731)
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| Project Period (FY) |
2005 – 2006
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| Keywords | B cell receptor / Crosslinking / Antibody |
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| Research Abstract |
Aim of this study is to set up an experimental system in which we can able to regulate condition of crosslinking of B cell receptor. For this purpose we tried to create "Fab-bridge". Basic concept of Fab-bridge is to connect two Fab portions of anti-mouse IgM monoclonal antibody using linker with different length. Firstly, we planned to raise monoclonal antibody to Fc-portion of myeloma IgM. Although it is considered to be difficult to prepare Fc portion of IgM by papain digestion, Dombrink-Kurzman and Voss reported the Fc preparation by low-temperature papain digestion (Molecular Immunology, 25, 1309-1320(1988)). However, we could not get Fc of IgM by papain-digestion from our IgM. During generation of the Fab portion, Fc portion was completely degraded. So we raised monoclonal antibody against whole IgM. We succeeded in obtaining four monoclonal antibodies. Currently, we are evaluating the ability of these antibody to stimulate mouse splenic B cells.
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