Elucidation of antigen recognition mechanism by antibody and conformational change of antibody upon antigen binding in correlation with protein fluctuation in solution

2008 Fiscal Year Final Research Report

• Project/Area Number: 17570096
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto Prefectural University
• Principal Investigator: ODA Masayuki Kyoto Prefectural University, 大学院・生命環境科学研究科, 准教授 (20318231)
• Project Period (FY): 2005 – 2008
• Keywords: 生体分子 / 蛋白質 / 分析化学 / 免疫学

Research Abstract

いくつかの抗原抗体間相互作用系を対象として、溶液中での蛋白質の動的挙動が反映される熱力学量や速度論量を主な指標として、静的な構造情報を補完する一般側の導出や方法論有効性の検証を目指した。抗体の抗原認識は多種多様であり、その解析から分子認識の一般側が得られ、さらに複数の抗原結合部位の可動性を解析することで、抗原上の比較的小さな構造変化から大きな構造変化にいたる動的挙動に関する結果が得られた。

Research Products

• [Journal Article] Exploring the energy landscape of antibody-antigen complexes : Protein dynamics, flexibility, and molecular recognition (2008)
  • Author(s): Thielges, M.C., Zimmermann, J., Yu, W., Oda, M., and Romesberg, F.E.
  • Journal Title: Biochemistry 47 Pages: 7237-7247
  • Peer Reviewed
• [Journal Article] Regional and segmental flexibility of antibodies in the interaction with antigens of different size (2006)
  • Author(s): Oda, M., Uchiyama, S., Robinson, C.V., Fukui, K., Kobayashi, Y., and Azuma, T.
  • Journal Title: FEBS J 273 Pages: 1476-1487
  • Peer Reviewed
• [Journal Article] Contribution of the trifluoroacetyl group in the thermodynamics of antigen-antibody binding
  • Author(s): Oda, M., Saito, M., Tsumuraya, T, and Fujii, I.
  • Journal Title: J. Mol. Recognit (in press)
  • Peer Reviewed
• [Presentation] 触媒抗体7C8と6D9の基質認識の比較 (2008)
  • Author(s): 織田昌幸
  • Organizer: 第44回熱測定討論会
  • Place of Presentation: つくば
  • Year and Date: 2008-10-17
• [Presentation] Direct observation of antigen-antibody complex : Effects of antigen size, valence, and binding affinity of antibody (2007)
  • Author(s): Maiko Koga
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-22
• [Presentation] Molecular basis of antigen-antibody interaction critical for B-cell activation (2007)
  • Author(s): Aki Kitai
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: 東京
  • Year and Date: 2007-11-20
• [Presentation] Interactions of antibodies with antigens of different sizes and valences (2006)
  • Author(s): Maiko Koga
  • Organizer: 第36回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2006-12-12
• [Presentation] 抗リゾチーム抗体の抗原認識能とエピトープ解析 (2006)
  • Author(s): 北井麻希
  • Organizer: 日本生物高分子学会2006年度大会
  • Place of Presentation: 京都
  • Year and Date: 2006-09-22