2006 Fiscal Year Final Research Report Summary
High accuracy function analysis of mitochondria with organelle adsorption method
| Project/Area Number |
17570129
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
OHTA Yoshihiro Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Associate Professor, 大学院共生科学技術研究院, 助教授 (10223843)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Hitoshi Chubu University, College of Life and Health Sciences, Professor, 生命健康科学部, 教授 (20342967)
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| Project Period (FY) |
2005 – 2006
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| Keywords | mitochondria / biophysics / bioimaging / reactive oxygen species / uncoupling protein 2 / cell death |
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| Research Abstract |
Purpose:
Developments of the method for measuring organelles' functions are not enough, although their functions are very important. Organelle adsorption method is a newly developed method which enables us to measure the activity of organelles adsorbed on the base. The characteristics are the measurements of individual organelles and the quick separation of the metabolites from the organelle. In this study, we measured mitochondrial behaviors with organelle adsorption method. Results and Discussions:
We measured the effects of 1) the uncoupling protein 2 (UCP2), 2) cyclophilin D,3) ganglioside GD3, 4) Ca2+, 5) respiration substrates on mitochondrial behaviors, and published 4 original papers in international journal and one review in domestic journal. The results are 1) UCP2 and cyclophilin D protect mitochondria from oxidative stress-induced damage, 2) GD3 promotes the generation of reactive oxygen species from mitochondria and induces the gradual depolarization, 3) mitochondrial membrane potential are stabilized in the cell by the presence of ADP, ATP and Mg2+, 4) Ca2+ induced the swelling of depolarized mitochondria not but polarized mitochondria. These results provide us the important information on mitochondrial behaviors.
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[Journal Article] Enhancement of nitric oxide production by association of nitric oxide synthase with N-methyl-d-aspartate receptors via postsynaptic density 95 in genetically engineered Chinese hamster ovary cells : real-time fluorescence imaging using nitric oxide sensitive dye2006
Author(s)
Ishii, H., Shibuya, K., Ohta, Y., Mukai, H., Uchino, S., Takata, N., Rose, J.A., Kawato, S.
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Journal Title
Journal of Neurochemistry 96
Pages: 1531-1539
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Enhancementof nitric oxide production by association of nitric oxidesynthase with N-methyl-d-aspartate receptors viapostsynaptic density 95 in genetically engineeredChinese hamster ovary cells : real-time fluorescenceimaging using nitric oxide sensitive dye2006
Author(s)
Ishii, H., Shibuya, K., Ohta, Y., Mukai, H., Uchino, S., Takata, N., Rose, J.A., Kawato, S.
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Journal Title
Journal of Neurochemistry 96
Pages: 1531-1539
Description
「研究成果報告書概要(欧文)」より
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